Q03112 (EVI1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MDS1 and EVI1 complex locus protein EVI1

Alternative name(s):
Ecotropic virus integration site 1 protein homolog
Short name=EVI-1

Gene names

Name:	MECOM
Synonyms:	EVI1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1051 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis. Ref.9 Ref.10 Ref.11 Ref.12 Ref.14 Ref.16
Subunit structure	Homooligomer. Interacts with SUV39H1 (via SET domain); enhances MECOM transcriptional repression activity By similarity. Interacts with CTBP1. Interacts with SMAD3 (via MH2 domain); the interaction is direct. Interacts with SMAD4; through interaction with SMAD3. Interacts with CREBBP, KAT2B and histone deacetylases. Interacts with MAPK8 and MAPK9; inhibits JNK signaling. Ref.9 Ref.10 Ref.11 Ref.12
Subcellular location	Nucleus. Nucleus speckle Ref.9 Ref.11 Ref.12.
Domain	Both zinc finger regions are required for the transcriptional activation of PBX1.
Post-translational modification	Phosphorylated By similarity. May be acetylated by CREBBP and KAT2B.
Involvement in disease	A chromosomal aberration involving EVI1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with RUNX1/AML1.
Sequence similarities	Contains 10 C2H2-type zinc fingers.
Sequence caution	The sequence AAB29907.1 differs from that shown. Reason: Erroneous initiation. The sequence AAB37456.1 differs from that shown. Reason: Probable cloning artifact. The sequence AAI30521.1 differs from that shown. Reason: Erroneous initiation. The sequence BAH14103.1 differs from that shown. Reason: Aberrant splicing.

Ontologies

Keywords
Biological process	Apoptosis Differentiation Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative promoter usage Alternative splicing Chromosomal rearrangement Polymorphism
Disease	Proto-oncogene
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW embryonic forelimb morphogenesis Inferred from electronic annotation. Source: Compara embryonic hindlimb morphogenesis Inferred from electronic annotation. Source: Compara forebrain development Inferred from electronic annotation. Source: Compara hematopoietic stem cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB in utero embryonic development Inferred from electronic annotation. Source: Compara inflammatory response Inferred from electronic annotation. Source: Compara negative regulation of JNK cascade Inferred from mutant phenotype Ref.10. Source: UniProtKB negative regulation of programmed cell death Inferred from mutant phenotype Ref.10. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.10Ref.11. Source: UniProtKB neutrophil homeostasis Inferred from electronic annotation. Source: Compara pericardium development Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from direct assay Ref.11Ref.16. Source: UniProtKB post-embryonic development Inferred from electronic annotation. Source: Compara regulation of cell cycle Inferred from direct assay Ref.11. Source: UniProtKB regulation of cell proliferation Inferred from electronic annotation. Source: Compara renal system development Inferred from electronic annotation. Source: Compara response to bacterium Inferred from electronic annotation. Source: Compara response to external stimulus Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nuclear speck Inferred from direct assay Ref.11. Source: UniProtKB
Molecular_function	DNA binding Inferred from sequence or structural similarity. Source: UniProtKB protein homodimerization activity Inferred from direct assay Ref.12. Source: UniProtKB sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.16. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ctbp2	P56546	3	EBI-1384862,EBI-1384883	From a different organism.
SIRT1	Q96EB6	2	EBI-1384862,EBI-1802965
UXT	Q9UBK9	5	EBI-1384862,EBI-357355

Alternative products

This entry describes 6 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q03112-1)

Also known as: Long; Evi-1a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q03112-3)

Also known as: Evi-1c; Mds1/Evi1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRSKGRARKL...APGEELLLFM

Note: Produced by alternative promoter usage. Contains an additional SET domain at positions 79-194. Unable to form homooligomers, to interact with CTBP1 and SMAD3 and to repress TGF-beta signaling.

Isoform 3 (identifier: Q13465-1)

Also known as: Mds1;

The sequence of this isoform can be found in the external entry Q13465.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative promoter usage.

Isoform 4 (identifier: Q03112-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MILDEFYNVKFCIDASQPDVGSWLKYIRFAGCYDQHNLVACQINDQIFYRVVADIAPGEELLLFM
     138-138: K → KQ

Isoform 5 (identifier: Q03112-5)

The sequence of this isoform differs from the canonical sequence as follows:
     672-680: Missing.

Isoform 6 (identifier: Q03112-6)

The sequence of this isoform differs from the canonical sequence as follows:
     138-138: K → KQ
     672-680: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1051	1051	MDS1 and EVI1 complex locus protein EVI1		PRO_0000047273
Regions
	Zinc finger	21 – 44	24	C2H2-type 1
	Zinc finger	75 – 97	23	C2H2-type 2
	Zinc finger	103 – 125	23	C2H2-type 3
	Zinc finger	131 – 154	24	C2H2-type 4
	Zinc finger	160 – 182	23	C2H2-type 5
	Zinc finger	188 – 210	23	C2H2-type 6
	Zinc finger	217 – 239	23	C2H2-type 7
	Zinc finger	733 – 755	23	C2H2-type 8
	Zinc finger	761 – 784	24	C2H2-type 9
	Zinc finger	790 – 812	23	C2H2-type 10
	Region	1 – 252	252	Interaction with MAPK9, SMAD3 and probably SUV39H1
	Motif	421 – 434	14	Nuclear localization signal Potential
	Motif	553 – 557	5	CTBP-binding motif 1 By similarity
	Motif	584 – 588	5	CTBP-binding motif 2 By similarity
	Compositional bias	886 – 937	52	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	854	1	Phosphoserine By similarity
	Modified residue	858	1	Phosphoserine By similarity
	Modified residue	860	1	Phosphoserine Ref.17
Natural variations
	Alternative sequence	1	1	M → MRSKGRARKLATNNECVYGN YPEIPLEEMPDADGVASTPS LNIQEPCSPATSSEAFTPKE GSPYKAPIYIPDDIPIPAEF ELRESNMPGAGLGIWTKRKI EVGEKFGPYVGEQRSNLKDP SYGWEILDEFYNVKFCIDAS QPDVGSWLKYIRFAGCYDQH NLVACQINDQIFYRVVADIA PGEELLLFM in isoform 2.		VSP_038733
	Alternative sequence	1	1	M → MILDEFYNVKFCIDASQPDV GSWLKYIRFAGCYDQHNLVA CQINDQIFYRVVADIAPGEE LLLFM in isoform 4.		VSP_038734
	Alternative sequence	138	1	K → KQ in isoform 4 and isoform 6.		VSP_038735
	Alternative sequence	672 – 680	9	Missing in isoform 5 and isoform 6.		VSP_038736
	Natural variant	107	1	Q → R. Corresponds to variant rs34896995 [ dbSNP | Ensembl ].		VAR_061928
Experimental info
	Mutagenesis	555 – 556	2	DL → AS: Partial loss of interaction with CTBP1. Loss of interaction with CTBP1; when associated with 586-A-S-587. Ref.11
	Mutagenesis	586 – 587	2	DL → AS: Partial loss of interaction with CTBP1. Loss of interaction with CTBP1; when associated with 555-A-S-556. Ref.11
	Sequence conflict	20	1	Q → R in CAE45952. Ref.4
	Sequence conflict	175	1	L → P in CAE45952. Ref.4
	Sequence conflict	301	1	F → S in BAF85554. Ref.3
	Sequence conflict	303	1	F → V in CAA38735. Ref.1
	Sequence conflict	443	1	S → P in CAI46086. Ref.4
	Sequence conflict	543	1	K → R in CAE45952. Ref.4
	Sequence conflict	730	1	K → R in BAF85554. Ref.3
	Sequence conflict	741	1	I → V in CAI46086. Ref.4
	Sequence conflict	796	1	D → Y in CAA38735. Ref.1
	Sequence conflict	875	1	D → E in CAA38735. Ref.1
	Sequence conflict	881	1	T → P in CAA38735. Ref.1
	Sequence conflict	906	1	N → Y in CAA38735. Ref.1
	Sequence conflict	992	1	V → A in BAF85554. Ref.3
	Sequence conflict	1013	1	L → P in CAE45952. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) (Evi-1a) [UniParc]. Last modified April 17, 2007. Version 2. Checksum: BD132C53EA08D263	FASTA	1,051	118,276
	10 20 30 40 50 60 MKSEDYPHET MAPDIHEERQ YRCEDCDQLF ESKAELADHQ KFPCSTPHSA FSMVEEDFQQ 70 80 90 100 110 120 KLESENDLQE IHTIQECKEC DQVFPDLQSL EKHMLSHTEE REYKCDQCPK AFNWKSNLIR 130 140 150 160 170 180 HQMSHDSGKH YECENCAKVF TDPSNLQRHI RSQHVGARAH ACPECGKTFA TSSGLKQHKH 190 200 210 220 230 240 IHSSVKPFIC EVCHKSYTQF SNLCRHKRMH ADCRTQIKCK DCGQMFSTTS SLNKHRRFCE 250 260 270 280 290 300 GKNHFAAGGF FGQGISLPGT PAMDKTSMVN MSHANPGLAD YFGANRHPAG LTFPTAPGFS 310 320 330 340 350 360 FSFPGLFPSG LYHRPPLIPA SSPVKGLSST EQTNKSQSPL MTHPQILPAT QDILKALSKH 370 380 390 400 410 420 PSVGDNKPVE LQPERSSEER PFEKISDQSE SSDLDDVSTP SGSDLETTSG SDLESDIESD 430 440 450 460 470 480 KEKFKENGKM FKDKVSPLQN LASINNKKEY SNHSIFSPSL EEQTAVSGAV NDSIKAIASI 490 500 510 520 530 540 AEKYFGSTGL VGLQDKKVGA LPYPSMFPLP FFPAFSQSMY PFPDRDLRSL PLKMEPQSPG 550 560 570 580 590 600 EVKKLQKGSS ESPFDLTTKR KDEKPLTPVP SKPPVTPATS QDQPLDLSMG SRSRASGTKL 610 620 630 640 650 660 TEPRKNHVFG GKKGSNVESR PASDGSLQHA RPTPFFMDPI YRVEKRKLTD PLEALKEKYL 670 680 690 700 710 720 RPSPGFLFHP QFQLPDQRTW MSAIENMAEK LESFSALKPE ASELLQSVPS MFNFRAPPNA 730 740 750 760 770 780 LPENLLRKGK ERYTCRYCGK IFPRSANLTR HLRTHTGEQP YRCKYCDRSF SISSNLQRHV 790 800 810 820 830 840 RNIHNKEKPF KCHLCDRCFG QQTNLDRHLK KHENGNMSGT ATSSPHSELE STGAILDDKE 850 860 870 880 890 900 DAYFTEIRNF IGNSNHGSQS PRNVEERMNG SHFKDEKALV TSQNSDLLDD EEVEDEVLLD 910 920 930 940 950 960 EEDEDNDITG KTGKEPVTSN LHEGNPEDDY EETSALEMSC KTSPVRYKEE EYKSGLSALD 970 980 990 1000 1010 1020 HIRHFTDSLK MRKMEDNQYS EAELSSFSTS HVPEELKQPL HRKSKSQAYA MMLSLSDKES 1030 1040 1050 LHSTSHSSSN VWHSMARAAA ESSAIQSISH V
	Isoform 2 (Evi-1c) (Mds1/Evi1) [UniParc]. Checksum: E8A5EAD310102D53	FASTA	1,239	139,308
	10 20 30 40 50 60 MRSKGRARKL ATNNECVYGN YPEIPLEEMP DADGVASTPS LNIQEPCSPA TSSEAFTPKE 70 80 90 100 110 120 GSPYKAPIYI PDDIPIPAEF ELRESNMPGA GLGIWTKRKI EVGEKFGPYV GEQRSNLKDP 130 140 150 160 170 180 SYGWEILDEF YNVKFCIDAS QPDVGSWLKY IRFAGCYDQH NLVACQINDQ IFYRVVADIA 190 200 210 220 230 240 PGEELLLFMK SEDYPHETMA PDIHEERQYR CEDCDQLFES KAELADHQKF PCSTPHSAFS 250 260 270 280 290 300 MVEEDFQQKL ESENDLQEIH TIQECKECDQ VFPDLQSLEK HMLSHTEERE YKCDQCPKAF 310 320 330 340 350 360 NWKSNLIRHQ MSHDSGKHYE CENCAKVFTD PSNLQRHIRS QHVGARAHAC PECGKTFATS 370 380 390 400 410 420 SGLKQHKHIH SSVKPFICEV CHKSYTQFSN LCRHKRMHAD CRTQIKCKDC GQMFSTTSSL 430 440 450 460 470 480 NKHRRFCEGK NHFAAGGFFG QGISLPGTPA MDKTSMVNMS HANPGLADYF GANRHPAGLT 490 500 510 520 530 540 FPTAPGFSFS FPGLFPSGLY HRPPLIPASS PVKGLSSTEQ TNKSQSPLMT HPQILPATQD 550 560 570 580 590 600 ILKALSKHPS VGDNKPVELQ PERSSEERPF EKISDQSESS DLDDVSTPSG SDLETTSGSD 610 620 630 640 650 660 LESDIESDKE KFKENGKMFK DKVSPLQNLA SINNKKEYSN HSIFSPSLEE QTAVSGAVND 670 680 690 700 710 720 SIKAIASIAE KYFGSTGLVG LQDKKVGALP YPSMFPLPFF PAFSQSMYPF PDRDLRSLPL 730 740 750 760 770 780 KMEPQSPGEV KKLQKGSSES PFDLTTKRKD EKPLTPVPSK PPVTPATSQD QPLDLSMGSR 790 800 810 820 830 840 SRASGTKLTE PRKNHVFGGK KGSNVESRPA SDGSLQHARP TPFFMDPIYR VEKRKLTDPL 850 860 870 880 890 900 EALKEKYLRP SPGFLFHPQF QLPDQRTWMS AIENMAEKLE SFSALKPEAS ELLQSVPSMF 910 920 930 940 950 960 NFRAPPNALP ENLLRKGKER YTCRYCGKIF PRSANLTRHL RTHTGEQPYR CKYCDRSFSI 970 980 990 1000 1010 1020 SSNLQRHVRN IHNKEKPFKC HLCDRCFGQQ TNLDRHLKKH ENGNMSGTAT SSPHSELEST 1030 1040 1050 1060 1070 1080 GAILDDKEDA YFTEIRNFIG NSNHGSQSPR NVEERMNGSH FKDEKALVTS QNSDLLDDEE 1090 1100 1110 1120 1130 1140 VEDEVLLDEE DEDNDITGKT GKEPVTSNLH EGNPEDDYEE TSALEMSCKT SPVRYKEEEY 1150 1160 1170 1180 1190 1200 KSGLSALDHI RHFTDSLKMR KMEDNQYSEA ELSSFSTSHV PEELKQPLHR KSKSQAYAMM 1210 1220 1230 LSLSDKESLH STSHSSSNVW HSMARAAAES SAIQSISHV
	Isoform 3 (Mds1) [UniParc]. See Q13465.		–	–
	Isoform 4 [UniParc]. Checksum: 772D3F793270B394	FASTA	1,116	125,807
	10 20 30 40 50 60 MILDEFYNVK FCIDASQPDV GSWLKYIRFA GCYDQHNLVA CQINDQIFYR VVADIAPGEE 70 80 90 100 110 120 LLLFMKSEDY PHETMAPDIH EERQYRCEDC DQLFESKAEL ADHQKFPCST PHSAFSMVEE 130 140 150 160 170 180 DFQQKLESEN DLQEIHTIQE CKECDQVFPD LQSLEKHMLS HTEEREYKCD QCPKAFNWKS 190 200 210 220 230 240 NLIRHQMSHD SGKHYECENC AKQVFTDPSN LQRHIRSQHV GARAHACPEC GKTFATSSGL 250 260 270 280 290 300 KQHKHIHSSV KPFICEVCHK SYTQFSNLCR HKRMHADCRT QIKCKDCGQM FSTTSSLNKH 310 320 330 340 350 360 RRFCEGKNHF AAGGFFGQGI SLPGTPAMDK TSMVNMSHAN PGLADYFGAN RHPAGLTFPT 370 380 390 400 410 420 APGFSFSFPG LFPSGLYHRP PLIPASSPVK GLSSTEQTNK SQSPLMTHPQ ILPATQDILK 430 440 450 460 470 480 ALSKHPSVGD NKPVELQPER SSEERPFEKI SDQSESSDLD DVSTPSGSDL ETTSGSDLES 490 500 510 520 530 540 DIESDKEKFK ENGKMFKDKV SPLQNLASIN NKKEYSNHSI FSPSLEEQTA VSGAVNDSIK 550 560 570 580 590 600 AIASIAEKYF GSTGLVGLQD KKVGALPYPS MFPLPFFPAF SQSMYPFPDR DLRSLPLKME 610 620 630 640 650 660 PQSPGEVKKL QKGSSESPFD LTTKRKDEKP LTPVPSKPPV TPATSQDQPL DLSMGSRSRA 670 680 690 700 710 720 SGTKLTEPRK NHVFGGKKGS NVESRPASDG SLQHARPTPF FMDPIYRVEK RKLTDPLEAL 730 740 750 760 770 780 KEKYLRPSPG FLFHPQFQLP DQRTWMSAIE NMAEKLESFS ALKPEASELL QSVPSMFNFR 790 800 810 820 830 840 APPNALPENL LRKGKERYTC RYCGKIFPRS ANLTRHLRTH TGEQPYRCKY CDRSFSISSN 850 860 870 880 890 900 LQRHVRNIHN KEKPFKCHLC DRCFGQQTNL DRHLKKHENG NMSGTATSSP HSELESTGAI 910 920 930 940 950 960 LDDKEDAYFT EIRNFIGNSN HGSQSPRNVE ERMNGSHFKD EKALVTSQNS DLLDDEEVED 970 980 990 1000 1010 1020 EVLLDEEDED NDITGKTGKE PVTSNLHEGN PEDDYEETSA LEMSCKTSPV RYKEEEYKSG 1030 1040 1050 1060 1070 1080 LSALDHIRHF TDSLKMRKME DNQYSEAELS SFSTSHVPEE LKQPLHRKSK SQAYAMMLSL 1090 1100 1110 SDKESLHSTS HSSSNVWHSM ARAAAESSAI QSISHV
	Isoform 5 [UniParc]. Checksum: 97476C1D04DB2007	FASTA	1,042	117,104
	10 20 30 40 50 60 MKSEDYPHET MAPDIHEERQ YRCEDCDQLF ESKAELADHQ KFPCSTPHSA FSMVEEDFQQ 70 80 90 100 110 120 KLESENDLQE IHTIQECKEC DQVFPDLQSL EKHMLSHTEE REYKCDQCPK AFNWKSNLIR 130 140 150 160 170 180 HQMSHDSGKH YECENCAKVF TDPSNLQRHI RSQHVGARAH ACPECGKTFA TSSGLKQHKH 190 200 210 220 230 240 IHSSVKPFIC EVCHKSYTQF SNLCRHKRMH ADCRTQIKCK DCGQMFSTTS SLNKHRRFCE 250 260 270 280 290 300 GKNHFAAGGF FGQGISLPGT PAMDKTSMVN MSHANPGLAD YFGANRHPAG LTFPTAPGFS 310 320 330 340 350 360 FSFPGLFPSG LYHRPPLIPA SSPVKGLSST EQTNKSQSPL MTHPQILPAT QDILKALSKH 370 380 390 400 410 420 PSVGDNKPVE LQPERSSEER PFEKISDQSE SSDLDDVSTP SGSDLETTSG SDLESDIESD 430 440 450 460 470 480 KEKFKENGKM FKDKVSPLQN LASINNKKEY SNHSIFSPSL EEQTAVSGAV NDSIKAIASI 490 500 510 520 530 540 AEKYFGSTGL VGLQDKKVGA LPYPSMFPLP FFPAFSQSMY PFPDRDLRSL PLKMEPQSPG 550 560 570 580 590 600 EVKKLQKGSS ESPFDLTTKR KDEKPLTPVP SKPPVTPATS QDQPLDLSMG SRSRASGTKL 610 620 630 640 650 660 TEPRKNHVFG GKKGSNVESR PASDGSLQHA RPTPFFMDPI YRVEKRKLTD PLEALKEKYL 670 680 690 700 710 720 RPSPGFLFHP QMSAIENMAE KLESFSALKP EASELLQSVP SMFNFRAPPN ALPENLLRKG 730 740 750 760 770 780 KERYTCRYCG KIFPRSANLT RHLRTHTGEQ PYRCKYCDRS FSISSNLQRH VRNIHNKEKP 790 800 810 820 830 840 FKCHLCDRCF GQQTNLDRHL KKHENGNMSG TATSSPHSEL ESTGAILDDK EDAYFTEIRN 850 860 870 880 890 900 FIGNSNHGSQ SPRNVEERMN GSHFKDEKAL VTSQNSDLLD DEEVEDEVLL DEEDEDNDIT 910 920 930 940 950 960 GKTGKEPVTS NLHEGNPEDD YEETSALEMS CKTSPVRYKE EEYKSGLSAL DHIRHFTDSL 970 980 990 1000 1010 1020 KMRKMEDNQY SEAELSSFST SHVPEELKQP LHRKSKSQAY AMMLSLSDKE SLHSTSHSSS 1030 1040 NVWHSMARAA AESSAIQSIS HV
	Isoform 6 [UniParc]. Checksum: B6A0605E499FF208	FASTA	1,043	117,232
	10 20 30 40 50 60 MKSEDYPHET MAPDIHEERQ YRCEDCDQLF ESKAELADHQ KFPCSTPHSA FSMVEEDFQQ 70 80 90 100 110 120 KLESENDLQE IHTIQECKEC DQVFPDLQSL EKHMLSHTEE REYKCDQCPK AFNWKSNLIR 130 140 150 160 170 180 HQMSHDSGKH YECENCAKQV FTDPSNLQRH IRSQHVGARA HACPECGKTF ATSSGLKQHK 190 200 210 220 230 240 HIHSSVKPFI CEVCHKSYTQ FSNLCRHKRM HADCRTQIKC KDCGQMFSTT SSLNKHRRFC 250 260 270 280 290 300 EGKNHFAAGG FFGQGISLPG TPAMDKTSMV NMSHANPGLA DYFGANRHPA GLTFPTAPGF 310 320 330 340 350 360 SFSFPGLFPS GLYHRPPLIP ASSPVKGLSS TEQTNKSQSP LMTHPQILPA TQDILKALSK 370 380 390 400 410 420 HPSVGDNKPV ELQPERSSEE RPFEKISDQS ESSDLDDVST PSGSDLETTS GSDLESDIES 430 440 450 460 470 480 DKEKFKENGK MFKDKVSPLQ NLASINNKKE YSNHSIFSPS LEEQTAVSGA VNDSIKAIAS 490 500 510 520 530 540 IAEKYFGSTG LVGLQDKKVG ALPYPSMFPL PFFPAFSQSM YPFPDRDLRS LPLKMEPQSP 550 560 570 580 590 600 GEVKKLQKGS SESPFDLTTK RKDEKPLTPV PSKPPVTPAT SQDQPLDLSM GSRSRASGTK 610 620 630 640 650 660 LTEPRKNHVF GGKKGSNVES RPASDGSLQH ARPTPFFMDP IYRVEKRKLT DPLEALKEKY 670 680 690 700 710 720 LRPSPGFLFH PQMSAIENMA EKLESFSALK PEASELLQSV PSMFNFRAPP NALPENLLRK 730 740 750 760 770 780 GKERYTCRYC GKIFPRSANL TRHLRTHTGE QPYRCKYCDR SFSISSNLQR HVRNIHNKEK 790 800 810 820 830 840 PFKCHLCDRC FGQQTNLDRH LKKHENGNMS GTATSSPHSE LESTGAILDD KEDAYFTEIR 850 860 870 880 890 900 NFIGNSNHGS QSPRNVEERM NGSHFKDEKA LVTSQNSDLL DDEEVEDEVL LDEEDEDNDI 910 920 930 940 950 960 TGKTGKEPVT SNLHEGNPED DYEETSALEM SCKTSPVRYK EEEYKSGLSA LDHIRHFTDS 970 980 990 1000 1010 1020 LKMRKMEDNQ YSEAELSSFS TSHVPEELKQ PLHRKSKSQA YAMMLSLSDK ESLHSTSHSS 1030 1040 SNVWHSMARA AAESSAIQSI SHV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Unique expression of the human Evi-1 gene in an endometrial carcinoma cell line: sequence of cDNAs and structure of alternatively spliced transcripts." Morishita K., Parganas E., Douglass E.C., Ihle J.N. Oncogene 5:963-971(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING.
[2]	"Generation of the AML1-EVI-1 fusion gene in the t(3;21)(q26;q22) causes blastic crisis in chronic myelocytic leukemia." Mitani K., Ogawa S., Tanaka T., Miyoshi H., Kurokawa M., Mano H., Yazaki Y., Ohki M., Hirai H. EMBO J. 13:504-510(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), CHROMOSOMAL TRANSLOCATION WITH RUNX1 IN CHRONIC MYELOCYTIC LEUKEMIA.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-759 (ISOFORM 2). Tissue: Trachea.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Tissue: Adipose tissue and Fetal kidney.
[5]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 4 AND 6), NUCLEOTIDE SEQUENCE [MRNA] OF 516-1051 (ISOFORM 5). Tissue: Lung.
[8]	"Structurally altered Evi-1 protein generated in the 3q21q26 syndrome." Ogawa S., Kurokawa M., Tanaka T., Mitani K., Inazawa J., Hangaishi A., Tanaka K., Matsuo Y., Minowada J., Tsubota T., Yazaki Y., Hirai H. Oncogene 13:183-191(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 970-1007 (ISOFORM 1).
[9]	"The oncoprotein Evi-1 represses TGF-beta signalling by inhibiting Smad3." Kurokawa M., Mitani K., Irie K., Matsuyama T., Takahashi T., Chiba S., Yazaki Y., Matsumoto K., Hirai H. Nature 394:92-96(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN TGF-BETA SIGNALING, INTERACTION WITH SMAD3 AND SMAD4, SUBCELLULAR LOCATION.
[10]	"The evi-1 oncoprotein inhibits c-Jun N-terminal kinase and prevents stress-induced cell death." Kurokawa M., Mitani K., Yamagata T., Takahashi T., Izutsu K., Ogawa S., Moriguchi T., Nishida E., Yazaki Y., Hirai H. EMBO J. 19:2958-2968(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MAPK8 AND MAPK9.
[11]	"Interaction of EVI1 with cAMP-responsive element-binding protein-binding protein (CBP) and p300/CBP-associated factor (P/CAF) results in reversible acetylation of EVI1 and in co-localization in nuclear speckles." Chakraborty S., Senyuk V., Sitailo S., Chi Y., Nucifora G. J. Biol. Chem. 276:44936-44943(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ACETYLATION, INTERACTION WITH CREBBP; CTBP1; KAT2B AND HISTONE DEACETYLASES, SUBCELLULAR LOCATION, MUTAGENESIS OF 555-ASP-LEU-556 AND 586-ASP-LEU-587.
[12]	"Oligomerization of Evi-1 regulated by the PR domain contributes to recruitment of corepressor CtBP." Nitta E., Izutsu K., Yamaguchi Y., Imai Y., Ogawa S., Chiba S., Kurokawa M., Hirai H. Oncogene 24:6165-6173(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ALTERNATIVE SPLICING (ISOFORMS 1 AND 2), HOMOOLIGOMERIZATION, INTERACTION WITH CTBP1 AND SMAD3, SUBCELLULAR LOCATION.
[13]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[14]	"Evi1 is a survival factor which conveys resistance to both TGFbeta- and taxol-mediated cell death via PI3K/AKT." Liu Y., Chen L., Ko T.C., Fields A.P., Thompson E.A. Oncogene 25:3565-3575(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN APOPTOSIS.
[15]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[16]	"Pbx1 is a downstream target of Evi-1 in hematopoietic stem/progenitors and leukemic cells." Shimabe M., Goyama S., Watanabe-Okochi N., Yoshimi A., Ichikawa M., Imai Y., Kurokawa M. Oncogene 28:4364-4374(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[17]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-860, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54989 mRNA. Translation: CAA38735.1. S69002 mRNA. Translation: AAB29907.1. Different initiation. AK292865 mRNA. Translation: BAF85554.1. AK304098 mRNA. Translation: BAH14103.1. Sequence problems. BX640908 mRNA. Translation: CAE45952.1. BX647613 mRNA. Translation: CAI46086.1. AC007849 Genomic DNA. No translation available. AC024099 Genomic DNA. No translation available. AC069220 Genomic DNA. No translation available. AC074033 Genomic DNA. No translation available. AC078985 Genomic DNA. No translation available. CH471052 Genomic DNA. Translation: EAW78553.1. CH471052 Genomic DNA. Translation: EAW78556.1. CH471052 Genomic DNA. Translation: EAW78557.1. BC031019 mRNA. Translation: AAH31019.1. BC130520 mRNA. Translation: AAI30521.1. Different initiation. BC143951 mRNA. Translation: AAI43952.1. BC143952 mRNA. Translation: AAI43953.1. S82592 mRNA. Translation: AAB37456.1. Sequence problems.
IPI	IPI00446866. IPI00790776. IPI00921346. IPI00943898. IPI00943944.
PIR	A60191. S41705.
RefSeq	NP_001098547.3. NM_001105077.3. NP_001098548.2. NM_001105078.3. NP_001157471.1. NM_001163999.1. NP_001157472.1. NM_001164000.1. NP_001192123.1. NM_001205194.1. NP_004982.2. NM_004991.3. NP_005232.2. NM_005241.3.
UniGene	Hs.659873.
3D structure databases
HSSP	HSSP built from PDB template 1SP2 based on UniProtKB P08047.
ProteinModelPortal	Q03112.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q03112. 3 interactions.
STRING	9606.ENSP00000264674.
PTM databases
PhosphoSite	Q03112.
Polymorphism databases
DMDM	145559472.
Proteomic databases
PaxDb	Q03112.
PRIDE	Q03112.
Protocols and materials databases
DNASU	2122.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000264674; ENSP00000264674; ENSG00000085276. ENST00000392736; ENSP00000376493; ENSG00000085276. ENST00000433243; ENSP00000394302; ENSG00000085276. ENST00000464456; ENSP00000419770; ENSG00000085276. ENST00000468789; ENSP00000419995; ENSG00000085276. ENST00000472280; ENSP00000420048; ENSG00000085276.
GeneID	2122.
KEGG	hsa:2122.
UCSC	uc003ffi.3. human. uc003ffj.3. human. uc003ffk.2. human. uc011bpi.1. human. uc011bpj.1. human.
Organism-specific databases
CTD	2122.
GeneCards	GC03M168802.
H-InvDB	HIX0003836.
HGNC	HGNC:3498. MECOM.
HPA	HPA046537.
MIM	165215. gene.
neXtProt	NX_Q03112.
Orphanet	52688. Myelodysplastic syndromes.
PharmGKB	PA27912.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOVERGEN	HBG005619.
InParanoid	Q03112.
KO	K04462.
PhylomeDB	Q03112.
Gene expression databases
ArrayExpress	Q03112.
Bgee	Q03112.
CleanEx	HS_EVI1.
Genevestigator	Q03112.
GermOnline	ENSG00000085276. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 8 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00096. zf-C2H2. 10 hits. [Graphical view]
SMART	SM00355. ZnF_C2H2. 10 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 8 hits. PS50157. ZINC_FINGER_C2H2_2. 10 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MECOM. human.
GenomeRNAi	2122.
NextBio	8579.
SOURCE	Search...

Entry information

Entry name

EVI1_HUMAN

Accession

Primary (citable) accession number: Q03112
Secondary accession number(s): A1L4F3 , A8KA00, B7Z8W7, B7ZLQ3, B7ZLQ4, C9JAK0, D3DNP7, Q16122, Q5HYI1, Q6MZS6, Q8NEI5, Q99917

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	April 17, 2007
Last modified:	May 1, 2013
This is version 130 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.