Reviewed, UniProtKB/Swiss-Prot Q03112 (EVI1_HUMAN)

Names and origin

Protein names

Recommended name:
    Ecotropic virus integration site 1 protein homolog
      Short name=EVI-1

Gene names

Name:

EVI1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1051 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	May interact with CTBP1 By similarity.
Subcellular location	Nucleus.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.4 Ref.5
Involvement in disease	A chromosomal aberration involving EVI1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with RUNX1/AML1.
Sequence similarities	Contains 10 C2H2-type zinc fingers.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Chromosomal rearrangement
Disease	Proto-oncogene
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Non-traceable author statement. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ctbp2	P56546	3	EBI-1384862,EBI-1384883	From a different organism.
UXT	Q9UBK9	4	EBI-1384862,EBI-357355

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]

Isoform 1 (identifier: Q03112-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q03112-2)

Also known as: Short;

The sequence of this isoform is not available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1051	1051	Ecotropic virus integration site 1 protein homolog		PRO_0000047273
Regions
	Zinc finger	21 – 44	24	C2H2-type 1
	Zinc finger	75 – 97	23	C2H2-type 2
	Zinc finger	103 – 125	23	C2H2-type 3
	Zinc finger	131 – 154	24	C2H2-type 4
	Zinc finger	160 – 182	23	C2H2-type 5
	Zinc finger	188 – 210	23	C2H2-type 6
	Zinc finger	217 – 239	23	C2H2-type 7
	Zinc finger	733 – 755	23	C2H2-type 8
	Zinc finger	761 – 784	24	C2H2-type 9
	Zinc finger	790 – 812	23	C2H2-type 10
	Motif	421 – 434	14	Nuclear localization signal Potential
	Motif	553 – 557	5	CTBP-binding motif 1 By similarity
	Motif	584 – 588	5	CTBP-binding motif 2 By similarity
	Compositional bias	886 – 937	52	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	854	1	Phosphoserine By similarity
	Modified residue	858	1	Phosphoserine Ref.4
	Modified residue	860	1	Phosphoserine Ref.4 Ref.5
Experimental info
	Sequence conflict	303	1	F → V in CAA38735. Ref.1
	Sequence conflict	672 – 680	9	Missing in AAB29907. Ref.2
	Sequence conflict	796	1	D → Y in CAA38735. Ref.1
	Sequence conflict	875	1	D → E in CAA38735. Ref.1
	Sequence conflict	881	1	T → P in CAA38735. Ref.1
	Sequence conflict	906	1	N → Y in CAA38735. Ref.1
	Sequence conflict	1008 – 1012	5	AYAMM → VQIFP in AAB37456. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) [UniParc]. Last modified April 17, 2007. Version 2. Checksum: BD132C53EA08D263	FASTA	1,051	118,276
	10 20 30 40 50 60 MKSEDYPHET MAPDIHEERQ YRCEDCDQLF ESKAELADHQ KFPCSTPHSA FSMVEEDFQQ 70 80 90 100 110 120 KLESENDLQE IHTIQECKEC DQVFPDLQSL EKHMLSHTEE REYKCDQCPK AFNWKSNLIR 130 140 150 160 170 180 HQMSHDSGKH YECENCAKVF TDPSNLQRHI RSQHVGARAH ACPECGKTFA TSSGLKQHKH 190 200 210 220 230 240 IHSSVKPFIC EVCHKSYTQF SNLCRHKRMH ADCRTQIKCK DCGQMFSTTS SLNKHRRFCE 250 260 270 280 290 300 GKNHFAAGGF FGQGISLPGT PAMDKTSMVN MSHANPGLAD YFGANRHPAG LTFPTAPGFS 310 320 330 340 350 360 FSFPGLFPSG LYHRPPLIPA SSPVKGLSST EQTNKSQSPL MTHPQILPAT QDILKALSKH 370 380 390 400 410 420 PSVGDNKPVE LQPERSSEER PFEKISDQSE SSDLDDVSTP SGSDLETTSG SDLESDIESD 430 440 450 460 470 480 KEKFKENGKM FKDKVSPLQN LASINNKKEY SNHSIFSPSL EEQTAVSGAV NDSIKAIASI 490 500 510 520 530 540 AEKYFGSTGL VGLQDKKVGA LPYPSMFPLP FFPAFSQSMY PFPDRDLRSL PLKMEPQSPG 550 560 570 580 590 600 EVKKLQKGSS ESPFDLTTKR KDEKPLTPVP SKPPVTPATS QDQPLDLSMG SRSRASGTKL 610 620 630 640 650 660 TEPRKNHVFG GKKGSNVESR PASDGSLQHA RPTPFFMDPI YRVEKRKLTD PLEALKEKYL 670 680 690 700 710 720 RPSPGFLFHP QFQLPDQRTW MSAIENMAEK LESFSALKPE ASELLQSVPS MFNFRAPPNA 730 740 750 760 770 780 LPENLLRKGK ERYTCRYCGK IFPRSANLTR HLRTHTGEQP YRCKYCDRSF SISSNLQRHV 790 800 810 820 830 840 RNIHNKEKPF KCHLCDRCFG QQTNLDRHLK KHENGNMSGT ATSSPHSELE STGAILDDKE 850 860 870 880 890 900 DAYFTEIRNF IGNSNHGSQS PRNVEERMNG SHFKDEKALV TSQNSDLLDD EEVEDEVLLD 910 920 930 940 950 960 EEDEDNDITG KTGKEPVTSN LHEGNPEDDY EETSALEMSC KTSPVRYKEE EYKSGLSALD 970 980 990 1000 1010 1020 HIRHFTDSLK MRKMEDNQYS EAELSSFSTS HVPEELKQPL HRKSKSQAYA MMLSLSDKES 1030 1040 1050 LHSTSHSSSN VWHSMARAAA ESSAIQSISH V
	Isoform 2 (Short) (Sequence not available).	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Unique expression of the human Evi-1 gene in an endometrial carcinoma cell line: sequence of cDNAs and structure of alternatively spliced transcripts." Morishita K., Parganas E., Douglass E.C., Ihle J.N. Oncogene 5:963-971(1990) [PubMed: 2115646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[2]	"Generation of the AML1-EVI-1 fusion gene in the t(3;21)(q26;q22) causes blastic crisis in chronic myelocytic leukemia." Mitani K., Ogawa S., Tanaka T., Miyoshi H., Kurokawa M., Mano H., Yazaki Y., Ohki M., Hirai H. EMBO J. 13:504-510(1994) [PubMed: 8313895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structurally altered Evi-1 protein generated in the 3q21q26 syndrome." Ogawa S., Kurokawa M., Tanaka T., Mitani K., Inazawa J., Hangaishi A., Tanaka K., Matsuo Y., Minowada J., Tsubota T., Yazaki Y., Hirai H. Oncogene 13:183-191(1996) [PubMed: 8700545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 970-1012.
[4]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-858 AND SER-860, MASS SPECTROMETRY.
[5]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-860, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54989 mRNA. Translation: CAA38735.1. S69002 mRNA. Translation: AAB29907.1. Sequence problems. S82592 mRNA. Translation: AAB37456.1.
IPI	IPI00446866.
PIR	A60191. S41705.
RefSeq	NP_001098548.2. NP_005232.2.
UniGene	Hs.714785
3D structure databases
HSSP	HSSP built from PDB template 1SP2 based on UniProtKB P08047.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q03112. 2 interactions.
PTM databases
PhosphoSite	Q03112.
Proteomic databases
PRIDE	Q03112.
Genome annotation databases
Ensembl	ENSG00000085276. Homo sapiens. [Contig view]
GeneID	2122.
NMPDR	fig|9606.3.peg.23473.
Organism-specific databases
GeneCards	GC03M170283.
H-InvDB	HIX0003836.
HGNC	HGNC:3498. EVI1.
MIM	165215. gene.
Orphanet	52688. Myelodysplastic syndromes.
PharmGKB	PA27912.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q03112.
HOVERGEN	Q03112.
OMA	Q03112. PAMDKTS.
Gene expression databases
ArrayExpress	Q03112.
Bgee	Q03112.
CleanEx	HS_EVI1.
GermOnline	ENSG00000085276. Homo sapiens.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 3 hits.
Pfam	PF00096. zf-C2H2. 10 hits. [Graphical view]
ProDom	PD000003. Znf_C2H2. 4 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00355. ZnF_C2H2. 10 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 8 hits. PS50157. ZINC_FINGER_C2H2_2. 10 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	8579.
SOURCE	Search...

Entry information

Entry name

EVI1_HUMAN

Accession

Primary (citable) accession number: Q03112
Secondary accession number(s): Q16122, Q99917

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	April 17, 2007
Last modified:	June 16, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)