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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lactococcus lactis subsp. cremoris (strain SK11)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei612 – 6121ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLLAC272622:GJUG-875-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:LACR_0875
OrganismiLactococcus lactis subsp. cremoris (strain SK11)
Taxonomic identifieri272622 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000000240: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 829829Leucine--tRNA ligasePRO_1000009360Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272622.LACR_0875.

Structurei

3D structure databases

ProteinModelPortaliQ030E7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi609 – 6135"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q030E7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEYNHQEIEA KWQKFWADNQ TFRTGTDKTK PKFYALDMFP YPSGAGLHVG
60 70 80 90 100
HPEGYTATDI LSRYKRAQGF NVLHPMGWDA FGLPAEQYAM DTGNDPAEFT
110 120 130 140 150
AENIANFKRQ INSLGFSYDW EREVNTTDPN FYKWTQWIFT KLYEKGLAYE
160 170 180 190 200
AEVAVNWVEE LGTAIANEEV LPDGTSERGG YPVVRKPMRQ WMLKITAYAE
210 220 230 240 250
RLLEDLEEVD WPESIKEMQR NWIGKSVGAD VTFKVEGTDK SFEVFTTRPD
260 270 280 290 300
TLFGATYAVL APEHDLVDAI TTPEQVQAVA DYRRQASLKS DLARTDLAKE
310 320 330 340 350
KTGAFTGAYA INPINGRKMP IWVADYVLAS YGHGAVMAVP AHDERDWEFA
360 370 380 390 400
KVYGLEILPV VEGGNVEEAV YTEDGPHINS EFLNGLDKAQ AIEKAIEFLE
410 420 430 440 450
EKKIGKKKIT YRLRDWLFSR QRYWGEPIPI IHWEDGTSTA LPENELPLVL
460 470 480 490 500
PVTSDIKPSG TGESPLANLT DWLEVTREDG VKGRRETNTM PQWAGSSWYY
510 520 530 540 550
LRYIDPHNTE ALADPELLKE WLPVDIYVGG AEHAVLHLLY ARFWHKVLYD
560 570 580 590 600
LGVVPTKEPF QKLFNQGMIL GTSYRDHRGA LVATDKVEKR DGGFYHMETG
610 620 630 640 650
EELEQAPAKM SKSLKNVVNP DDVVEHYGAD TLRVYEMFMG PLDASIPWSE
660 670 680 690 700
EGLEGARKFL DRVVRMIDNS EISDKNNGEL DKVYNETVKN VSERLDLMYF
710 720 730 740 750
NTAISQLMIF VNATNKAKIL PLEYANGFVQ LLAPFAPHIA EELWVKLGNE
760 770 780 790 800
AGISYVAWPT FDESKLVESE VEIVVQINGK LKAKIKIAKD LGRDELEKIG
810 820
REAVAEALEG KNVVKVIAVP NKLVNIVVK
Length:829
Mass (Da):93,895
Last modified:November 14, 2006 - v1
Checksum:i1C1600BBA51267E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000425 Genomic DNA. Translation: ABJ72425.1.
RefSeqiYP_808847.1. NC_008527.1.

Genome annotation databases

EnsemblBacteriaiABJ72425; ABJ72425; LACR_0875.
GeneIDi4433308.
KEGGillc:LACR_0875.
PATRICi22288559. VBILacLac38071_1030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000425 Genomic DNA. Translation: ABJ72425.1.
RefSeqiYP_808847.1. NC_008527.1.

3D structure databases

ProteinModelPortaliQ030E7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272622.LACR_0875.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ72425; ABJ72425; LACR_0875.
GeneIDi4433308.
KEGGillc:LACR_0875.
PATRICi22288559. VBILacLac38071_1030.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciLLAC272622:GJUG-875-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SK11.

Entry informationi

Entry nameiSYL_LACLS
AccessioniPrimary (citable) accession number: Q030E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: January 7, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.