Q03099 (YMN3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Y' element ATP-dependent helicase YML133C
EC=3.6.4.12

Gene names

Ordered Locus Names:	YML133C
ORF Names:	YM4987.01C, YM4987.02C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1374 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes DNA unwinding and is involved in telomerase-independent telomere maintenance. Ref.3
Catalytic activity	ATP + H2O = ADP + phosphate.
Induction	Induced in absence of telomerase TLC1. Ref.3
Sequence similarities	Belongs to the helicase family. Yeast subtelomeric Y' repeat subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence CAA90549.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA90550.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1374	1374	Y' element ATP-dependent helicase YML133C		PRO_0000102209
Regions
	Domain	375 – 552	178	Helicase ATP-binding
	Domain	609 – 758	150	Helicase C-terminal
	Nucleotide binding	388 – 395	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q03099 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 9867CAC68D2C497E	FASTA	1,374	155,553
	10 20 30 40 50 60 MKVSDRRKFE KANFDEFESA LNNKNDLVHC PSITLFESIP TEVRSFYEDE KSGLIKVVKF 70 80 90 100 110 120 RTGAMDRKRS FEKIVVSVMV GKNVQKFLTF VEDEPDFQGG PIPSKYLIPK KINLMVYTLF 130 140 150 160 170 180 QVHTLKFNRK DYDTLSLFYL NRGYYNELSF RVLERCHEIA SARPNDSSTM RTFTDFVSGA 190 200 210 220 230 240 PIVRSLQKST IRRYGYNLAP HMFLLLHVDE LSIFSAYQAS LPGEKKVDTE RLKRDLCPRK 250 260 270 280 290 300 PIEIKYFSQI CNDMMNKKDR LGDVLATAQR IRRRYNKNSS SEPRLKTLDG LTSERWIQWL 310 320 330 340 350 360 GLESDYHCSF SSTRNAEDVV AGEAASSDHH QKISRVTRKR PREPKSTNDI LVAGQKLFGS 370 380 390 400 410 420 SFEFRDLHQL RLCHEIYMAD TPSVAVQAPP GYGKTELFHL PLIALASKGD VKYVSFLFVP 430 440 450 460 470 480 YTVLLANCMI RLSRCGCLNV APVRNFIEEG CDGVTDLYVG IYDDLASTNF TDRIAAWENI 490 500 510 520 530 540 VECTFRTNNV KLGYLIVDEF HNFETEVYRQ SQFGGITNLD FDAFEKAIFL SGTAPEAVAD 550 560 570 580 590 600 AALQRIGLTG LAKKSMDINE LKRSEDLSRG LSSYPTRMFN LIKEKSEVPL GHVHKIWKKV 610 620 630 640 650 660 ESQPEEALKL LLALFEIEPE SKAIVVASTT NEVEELACSW RKYFRVVWIH GKLGAAEKVS 670 680 690 700 710 720 RTKEFVTDGS MRVLIGTKLV TEGIDIKQLM MVIMLDNRLN IIELIQGVGR LRDGGLCYLL 730 740 750 760 770 780 SRKNSWAARN RKGELPPIKE GCITEQVREF YGLESKKGKK GQHVGCCGSR TDLSADTVEL 790 800 810 820 830 840 IERMDRLAEK QATASMSIVA LPSSFQESNS SDRCRKYCSS DEDSDTCIHG SANASTNATT 850 860 870 880 890 900 NSSTNATTTA STNVRTSATT TASINVRTSA TTTESTNSST NATTTASTNV RTSATTTASI 910 920 930 940 950 960 NVRTSATTTE STNSNTSATT TESTDSNTSA TTTESTDSNT SATTTASTNS STNATTTAST 970 980 990 1000 1010 1020 NSSTNATTTE STNASAKEDA NKDGNAEDNR FHPVTDINKE SYKRKGSQMV LLERKKLKAQ 1030 1040 1050 1060 1070 1080 FPNTSENMNV LQFLGFRSDE IKHLFLYGID IYFCPEGVFT QYGLCKGCQK MFELCVCWAG 1090 1100 1110 1120 1130 1140 QKVSYRRMAW EALAVERMLR NDEEYKEYLE DIEPYHGDPV GYLKFFSVKR GEIYSQIQRN 1150 1160 1170 1180 1190 1200 YAWYLAITRR RETISVLDST RGKQGSQVFR MSGRQIKELY YKVWSNLRES KTEVLQYFLN 1210 1220 1230 1240 1250 1260 WDEKKCREEW EAKDDTVFVE ALEKVGVFQR LRSMTSAGLQ GPQYVKLQFS RHHRQLRSRY 1270 1280 1290 1300 1310 1320 ELSLGMHLRD QLALGVTPSK VPHWTAFLSM LIGLFYNKTF RQKLEYLLEQ ISEVWLLPHW 1330 1340 1350 1360 1370 VDLANVEVLA ADNTRVPLYM LMVAVHKELD SDDVPDGRFD IILLCRDSSR EVGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Y'-Help1, a DNA helicase encoded by the yeast subtelomeric Y' element, is induced in survivors defective for telomerase." Yamada M., Hayatsu N., Matsuura A., Ishikawa F. J. Biol. Chem. 273:33360-33366(1998) [PubMed: 9837911] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z50178 Genomic DNA. Translation: CAA90549.1. Sequence problems. Z50178 Genomic DNA. Translation: CAA90550.1. Sequence problems. BK006946 Genomic DNA. Translation: DAA09766.1.
PIR	S69875.
RefSeq	NP_013573.1. NM_001182496.1.
3D structure databases
ProteinModelPortal	Q03099.
SMR	Q03099. Positions 619-723.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7582N.
MINT	MINT-545653.
STRING	Q03099.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML133C; YML133C; YML133C.
GeneID	854906.
KEGG	sce:YML133C.
Organism-specific databases
CYGD	YML133c.
SGD	S000004602. YML133C.
Phylogenomic databases
eggNOG	fuNOG09714.
HOGENOM	HBG204022.
OrthoDB	EOG4V9Z0T.
Gene expression databases
Genevestigator	Q03099.
GermOnline	YML133C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR001650. Helicase_C. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977893.

Entry information

Entry name

YMN3_YEAST

Accession

Primary (citable) accession number: Q03099
Secondary accession number(s): D6W0F2, Q12054

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program