Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cbb3-type cytochrome c oxidase subunit FixP

Gene

fixP

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

C-type cytochrome. Part of the cbb3-type cytochrome c oxidase complex. FixP subunit is required for transferring electrons from donor cytochrome c via its heme groups to FixO subunit. From there, electrons are shuttled to the catalytic binuclear center of FixN subunit where oxygen reduction takes place. The complex also functions as a proton pump.By similarity6 Publications

Cofactori

heme cBy similarity3 PublicationsNote: Binds 2 heme C groups per subunit.By similarity3 Publications

Pathwayi: oxidative phosphorylation

This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei122Heme C 1 (covalent)By similarity1
Binding sitei125Heme C 1 (covalent)By similarity1
Metal bindingi126Iron (heme C 1 axial ligand)By similarity1
Metal bindingi173Iron (heme C 2 axial ligand)By similarity1
Binding sitei219Heme C 2 (covalent)By similarity1
Binding sitei222Heme C 2 (covalent)By similarity1
Metal bindingi223Iron (heme C 2 axial ligand)By similarity1
Metal bindingi264Iron (heme C 1 axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Hydrogen ion transport, Ion transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00705.

Names & Taxonomyi

Protein namesi
Recommended name:
Cbb3-type cytochrome c oxidase subunit FixPBy similarity
Short name:
Cbb3-Cox subunit FixPBy similarity
Alternative name(s):
C-type cytochrome FixPBy similarity
Short name:
Cyt c(FixP)By similarity
Cytochrome c oxidase subunit IIIImported
Gene namesi
Name:fixPImported
Ordered Locus Names:blr2766
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32CytoplasmicSequence analysisBy similarityAdd BLAST32
Transmembranei33 – 53HelicalSequence analysisAdd BLAST21
Topological domaini54 – 290PeriplasmicSequence analysisBy similarityAdd BLAST237

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Microaerobically grown cells show 38% decrease in cytochrome c oxadase complex activity. Between 0-5% nitrogen fixing activity of the nitrogenase in root nodules when in symbiosis with soybean.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi122C → S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-125 or with S-125, S-219 and S-222. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex. 1 Publication1
Mutagenesisi125C → S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-122 or with S-122, S-219 and S-222. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex. 1 Publication1
Mutagenesisi219C → S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-222 or with S-222, S-122 and S-125. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex. 1 Publication1
Mutagenesisi222C → S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-219 or with S-219, S-122 and S-125. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004122961 – 290Cbb3-type cytochrome c oxidase subunit FixPAdd BLAST290

Interactioni

Subunit structurei

Component of the cbb3-type cytochrome c oxidase at least composed of FixN, FixO, FixQ and FixP.By similarity3 Publications

Protein-protein interaction databases

STRINGi224911.blr2766.

Structurei

3D structure databases

ProteinModelPortaliQ03075.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 198Cytochrome c 1PROSITE-ProRule annotationAdd BLAST90
Domaini206 – 287Cytochrome c 2PROSITE-ProRule annotationAdd BLAST82

Sequence similaritiesi

Belongs to the CcoP / FixP family.Curated
Contains 2 cytochrome c domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D6P. Bacteria.
COG2010. LUCA.
HOGENOMiHOG000277941.
InParanoidiQ03075.
KOiK00406.
OMAiPIFKAYA.
OrthoDBiPOG091H05TP.
PhylomeDBiQ03075.

Family and domain databases

Gene3Di1.10.760.10. 3 hits.
InterProiIPR032858. CcoP_N.
IPR009056. Cyt_c-like_dom.
IPR008168. Cyt_C_IC.
IPR004678. Cyt_c_oxidase_cbb3_su3.
[Graphical view]
PfamiPF13442. Cytochrome_CBB3. 2 hits.
PF14715. FixP_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000006. Cbb3-Cox_fixP. 1 hit.
PRINTSiPR00605. CYTCHROMECIC.
SUPFAMiSSF46626. SSF46626. 3 hits.
TIGRFAMsiTIGR00782. ccoP. 1 hit.
PROSITEiPS51007. CYTC. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03075-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDHSEFDSV SGKTTTGHEW DGIKELNTPL PRWWVICFYL TIVWAIGYWI
60 70 80 90 100
VYPAWPLISS NTTGLFGYSS RADVAVELAN LEKIRGDKMA ALGAASLADV
110 120 130 140 150
EKDPALLALA RAKGKTVFGD NCAPCHGSGG AGAKGFPNLN DDDWLWGGTL
160 170 180 190 200
DQIMQTIQFG ARSGHAKTHE GQMLAFGKDG VLKGDEIVTV ANYVRSLSGL
210 220 230 240 250
PTRKGYDAAK GEKIFVENCV ACHGDGGKGN QEMGAPNLTD KIWLYGSDEA
260 270 280 290
ALIETISQGR AGVMPAWEGR LDPSTIKAMA VYVHSLGGGK
Length:290
Mass (Da):31,024
Last modified:November 1, 1996 - v1
Checksum:i983F1B606D507BAC
GO

Mass spectrometryi

Molecular mass is 32130 Da from positions 1 - 290. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07487 Genomic DNA. Translation: AAA26206.1.
AJ005001 Genomic DNA. Translation: CAA06282.1.
BA000040 Genomic DNA. Translation: BAC48031.1.
PIRiD47468.
RefSeqiNP_769406.1. NC_004463.1.
WP_011085551.1. NZ_CP011360.1.

Genome annotation databases

EnsemblBacteriaiBAC48031; BAC48031; BAC48031.
GeneIDi1054100.
KEGGibja:blr2766.
PATRICi21188980. VBIBraJap65052_2748.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07487 Genomic DNA. Translation: AAA26206.1.
AJ005001 Genomic DNA. Translation: CAA06282.1.
BA000040 Genomic DNA. Translation: BAC48031.1.
PIRiD47468.
RefSeqiNP_769406.1. NC_004463.1.
WP_011085551.1. NZ_CP011360.1.

3D structure databases

ProteinModelPortaliQ03075.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.blr2766.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC48031; BAC48031; BAC48031.
GeneIDi1054100.
KEGGibja:blr2766.
PATRICi21188980. VBIBraJap65052_2748.

Phylogenomic databases

eggNOGiENOG4105D6P. Bacteria.
COG2010. LUCA.
HOGENOMiHOG000277941.
InParanoidiQ03075.
KOiK00406.
OMAiPIFKAYA.
OrthoDBiPOG091H05TP.
PhylomeDBiQ03075.

Enzyme and pathway databases

UniPathwayiUPA00705.

Family and domain databases

Gene3Di1.10.760.10. 3 hits.
InterProiIPR032858. CcoP_N.
IPR009056. Cyt_c-like_dom.
IPR008168. Cyt_C_IC.
IPR004678. Cyt_c_oxidase_cbb3_su3.
[Graphical view]
PfamiPF13442. Cytochrome_CBB3. 2 hits.
PF14715. FixP_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000006. Cbb3-Cox_fixP. 1 hit.
PRINTSiPR00605. CYTCHROMECIC.
SUPFAMiSSF46626. SSF46626. 3 hits.
TIGRFAMsiTIGR00782. ccoP. 1 hit.
PROSITEiPS51007. CYTC. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFIXP_BRADU
AccessioniPrimary (citable) accession number: Q03075
Secondary accession number(s): D4AER5
, E7FH98, Q79UA4, Q89RK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.