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Protein

Cbb3-type cytochrome c oxidase subunit FixP

Gene

fixP

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

C-type cytochrome. Part of the cbb3-type cytochrome c oxidase complex. FixP subunit is required for transferring electrons from donor cytochrome c via its heme groups to FixO subunit. From there, electrons are shuttled to the catalytic binuclear center of FixN subunit where oxygen reduction takes place. The complex also functions as a proton pump.By similarity6 Publications

Cofactori

heme cBy similarity3 PublicationsNote: Binds 2 heme C groups per subunit.By similarity3 Publications

Pathwayi: oxidative phosphorylation

This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei122Heme C 1 (covalent)By similarity1
Binding sitei125Heme C 1 (covalent)By similarity1
Metal bindingi126Iron (heme C 1 axial ligand)By similarity1
Metal bindingi173Iron (heme C 2 axial ligand)By similarity1
Binding sitei219Heme C 2 (covalent)By similarity1
Binding sitei222Heme C 2 (covalent)By similarity1
Metal bindingi223Iron (heme C 2 axial ligand)By similarity1
Metal bindingi264Iron (heme C 1 axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Hydrogen ion transport, Ion transport, Respiratory chain, Transport
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBDIA224911:G1G3J-2790-MONOMER
UniPathwayiUPA00705

Names & Taxonomyi

Protein namesi
Recommended name:
Cbb3-type cytochrome c oxidase subunit FixPBy similarity
Short name:
Cbb3-Cox subunit FixPBy similarity
Alternative name(s):
C-type cytochrome FixPBy similarity
Short name:
Cyt c(FixP)By similarity
Cytochrome c oxidase subunit IIIImported
Gene namesi
Name:fixPImported
Ordered Locus Names:blr2766
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32CytoplasmicSequence analysisBy similarityAdd BLAST32
Transmembranei33 – 53HelicalSequence analysisAdd BLAST21
Topological domaini54 – 290PeriplasmicSequence analysisBy similarityAdd BLAST237

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Microaerobically grown cells show 38% decrease in cytochrome c oxadase complex activity. Between 0-5% nitrogen fixing activity of the nitrogenase in root nodules when in symbiosis with soybean.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi122C → S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-125 or with S-125, S-219 and S-222. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex. 1 Publication1
Mutagenesisi125C → S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-122 or with S-122, S-219 and S-222. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex. 1 Publication1
Mutagenesisi219C → S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-222 or with S-222, S-122 and S-125. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex. 1 Publication1
Mutagenesisi222C → S: 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex; when associated with S-219 or with S-219, S-122 and S-125. 85% decrease in cytochrome c oxidase complex activity and assembly defects of the complex. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004122961 – 290Cbb3-type cytochrome c oxidase subunit FixPAdd BLAST290

Interactioni

Subunit structurei

Component of the cbb3-type cytochrome c oxidase at least composed of FixN, FixO, FixQ and FixP.By similarity3 Publications

Protein-protein interaction databases

STRINGi224911.blr2766

Structurei

3D structure databases

ProteinModelPortaliQ03075
SMRiQ03075
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 198Cytochrome c 1PROSITE-ProRule annotationAdd BLAST90
Domaini206 – 287Cytochrome c 2PROSITE-ProRule annotationAdd BLAST82

Sequence similaritiesi

Belongs to the CcoP / FixP family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D6P Bacteria
COG2010 LUCA
HOGENOMiHOG000277941
InParanoidiQ03075
KOiK00406
OMAiTDNDWLY
OrthoDBiPOG091H05TP
PhylomeDBiQ03075

Family and domain databases

Gene3Di1.10.760.10, 3 hits
1.20.58.1490, 1 hit
InterProiView protein in InterPro
IPR032858 CcoP_N
IPR038414 CcoP_N_sf
IPR009056 Cyt_c-like_dom
IPR036909 Cyt_c-like_dom_sf
IPR008168 Cyt_C_IC
IPR004678 Cyt_c_oxidase_cbb3_su3
PfamiView protein in Pfam
PF13442 Cytochrome_CBB3, 2 hits
PF14715 FixP_N, 1 hit
PIRSFiPIRSF000006 Cbb3-Cox_fixP, 1 hit
PRINTSiPR00605 CYTCHROMECIC
SUPFAMiSSF46626 SSF46626, 3 hits
TIGRFAMsiTIGR00782 ccoP, 1 hit
PROSITEiView protein in PROSITE
PS51007 CYTC, 2 hits

Sequencei

Sequence statusi: Complete.

Q03075-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDHSEFDSV SGKTTTGHEW DGIKELNTPL PRWWVICFYL TIVWAIGYWI
60 70 80 90 100
VYPAWPLISS NTTGLFGYSS RADVAVELAN LEKIRGDKMA ALGAASLADV
110 120 130 140 150
EKDPALLALA RAKGKTVFGD NCAPCHGSGG AGAKGFPNLN DDDWLWGGTL
160 170 180 190 200
DQIMQTIQFG ARSGHAKTHE GQMLAFGKDG VLKGDEIVTV ANYVRSLSGL
210 220 230 240 250
PTRKGYDAAK GEKIFVENCV ACHGDGGKGN QEMGAPNLTD KIWLYGSDEA
260 270 280 290
ALIETISQGR AGVMPAWEGR LDPSTIKAMA VYVHSLGGGK
Length:290
Mass (Da):31,024
Last modified:November 1, 1996 - v1
Checksum:i983F1B606D507BAC
GO

Mass spectrometryi

Molecular mass is 32130 Da from positions 1 - 290. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07487 Genomic DNA Translation: AAA26206.1
AJ005001 Genomic DNA Translation: CAA06282.1
BA000040 Genomic DNA Translation: BAC48031.1
PIRiD47468
RefSeqiNP_769406.1, NC_004463.1
WP_011085551.1, NZ_CP011360.1

Genome annotation databases

EnsemblBacteriaiBAC48031; BAC48031; BAC48031
GeneIDi1054100
KEGGibja:blr2766
PATRICifig|224911.44.peg.2387

Similar proteinsi

Entry informationi

Entry nameiFIXP_BRADU
AccessioniPrimary (citable) accession number: Q03075
Secondary accession number(s): D4AER5
, E7FH98, Q79UA4, Q89RK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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