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Q03073 (FIXN_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome c oxidase subunit 1 homolog, bacteroid

EC=1.9.3.1
Alternative name(s):
Cytochrome CBB3 subunit 1
Cytochrome c oxidase polypeptide I homolog
Heme B/copper cytochrome c oxidase subunit
Gene names
Name:fixN
Ordered Locus Names:blr2763
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length549 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Co I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c or a quinol are transferred to the bimetallic center formed by a high-spin heme and copper B.

Catalytic activity

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathway

Energy metabolism; oxidative phosphorylation.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Developmental stage

Bacteroid (nitrogen-fixing endosymbiont).

Induction

By low oxygen levels.

Miscellaneous

This cytochrome oxidase is probably a cbb3-type.

Sequence similarities

Belongs to the heme-copper respiratory oxidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 549549Cytochrome c oxidase subunit 1 homolog, bacteroid
PRO_0000183469

Regions

Transmembrane12 – 3221Helical; Potential
Transmembrane39 – 5921Helical; Potential
Transmembrane87 – 10721Helical; Potential
Transmembrane132 – 15221Helical; Potential
Transmembrane168 – 18821Helical; Potential
Transmembrane201 – 22121Helical; Potential
Transmembrane228 – 24821Helical; Potential
Transmembrane279 – 29921Helical; Potential
Transmembrane312 – 33221Helical; Potential
Transmembrane344 – 36421Helical; Potential
Transmembrane382 – 40221Helical; Potential
Transmembrane423 – 44321Helical; Potential
Transmembrane458 – 47821Helical; Potential
Transmembrane512 – 53221Helical; Potential

Sites

Metal binding1311Iron (heme B axial ligand) Probable
Metal binding2801Copper B Probable
Metal binding3301Copper B Probable
Metal binding3311Copper B Probable
Metal binding4181Iron (high-spin heme B axial ligand) Probable
Metal binding4201Iron (heme B axial ligand) Probable

Sequences

Sequence LengthMass (Da)Tools
Q03073 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: EEFBB48F18C364E3

FASTA54961,273
        10         20         30         40         50         60 
MSQPSISKSM TIGESGLAVV FAATAFLCVI AAAKALDAPF AFHAALSAAA SVAAVFCIVN 

        70         80         90        100        110        120 
RYFERPAALP PAEINGRPNY NMGPIKFSSF MAMFWGIAGF LVGLIIASQL AWPALNFDLP 

       130        140        150        160        170        180 
WISFGRLRPL HTSAVIFAFG GNVLIATSFY VVQKSCRVRL AGDLAPWFVV VGYNFFILVA 

       190        200        210        220        230        240 
GTGYLLGVTQ SKEYAEPEWY ADLWLTIVWV VYLLVFLATI IKRKEPHIFV ANWFYLAFIV 

       250        260        270        280        290        300 
TIAVLHLGNN PALPVSAFGS KSYVAWGGIQ DAMFQWWYGH NAVGFFLTAG FLAIMYYFIP 

       310        320        330        340        350        360 
KRAERPIYSY RLSIIHFWAL IFLYIWAGPH HLHYTALPDW TQTLGMTFSI MLWMPSWGGM 

       370        380        390        400        410        420 
INGLMTLSGA WDKLRTDPVL RMLVVSVAFY GMSTFEGPMM SIKVVNSLSH YTDWTIGHVH 

       430        440        450        460        470        480 
SGALGWVGFV SFGALYCLVP WAWNRKGLYS LKLVNWHFWV ATLGIVLYIS AMWVSGILQG 

       490        500        510        520        530        540 
LMWRAYTSLG FLEYSFIETV EAMHPFYIIR AAGGGLFLIG ALIMAYNLWM TVRVGEAEVQ 


MPVALQPAE 

« Hide

References

« Hide 'large scale' references
[1]"Genes for a microaerobically induced oxidase complex in Bradyrhizobium japonicum are essential for a nitrogen-fixing endosymbiosis."
Preisig O., Anthamatten D., Hennecke H.
Proc. Natl. Acad. Sci. U.S.A. 90:3309-3313(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: USDA 110spc4.
[2]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L07487 Genomic DNA. Translation: AAA26203.1.
AJ005001 Genomic DNA. Translation: CAA06279.1.
BA000040 Genomic DNA. Translation: BAC48028.1.
PIRA47468.
RefSeqNP_769403.1. NC_004463.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.blr2763.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC48028; BAC48028; BAC48028.
GeneID1054103.
KEGGbja:blr2763.
PATRIC21188974. VBIBraJap65052_2745.

Phylogenomic databases

eggNOGCOG3278.
HOGENOMHOG000277908.
KOK00404.
OrthoDBEOG6G7QZX.
PhylomeDBQ03073.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-2787-MONOMER.
UniPathwayUPA00705.

Family and domain databases

Gene3D1.20.210.10. 1 hit.
InterProIPR000883. Cyt_c_Oxase_su1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
IPR004677. Cyt_c_oxidase_cbb3_su1.
[Graphical view]
PANTHERPTHR10422. PTHR10422. 1 hit.
PfamPF00115. COX1. 1 hit.
[Graphical view]
SUPFAMSSF81442. SSF81442. 1 hit.
TIGRFAMsTIGR00780. ccoN. 1 hit.
PROSITEPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFIXN_BRADU
AccessionPrimary (citable) accession number: Q03073
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways