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Protein

Cytochrome c oxidase subunit 1 homolog, bacteroid

Gene

fixN

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Co I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c or a quinol are transferred to the bimetallic center formed by a high-spin heme and copper B.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi131 – 1311Iron (heme B axial ligand)Curated
Metal bindingi280 – 2801Copper BCurated
Metal bindingi330 – 3301Copper BCurated
Metal bindingi331 – 3311Copper BCurated
Metal bindingi418 – 4181Iron (high-spin heme B axial ligand)Curated
Metal bindingi420 – 4201Iron (heme B axial ligand)Curated

GO - Molecular functioni

  1. cytochrome-c oxidase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. aerobic respiration Source: InterPro
  2. oxidative phosphorylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-2787-MONOMER.
UniPathwayiUPA00705.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 1 homolog, bacteroid (EC:1.9.3.1)
Alternative name(s):
Cytochrome CBB3 subunit 1
Cytochrome c oxidase polypeptide I homolog
Heme B/copper cytochrome c oxidase subunit
Gene namesi
Name:fixN
Ordered Locus Names:blr2763
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221HelicalSequence AnalysisAdd
BLAST
Transmembranei39 – 5921HelicalSequence AnalysisAdd
BLAST
Transmembranei87 – 10721HelicalSequence AnalysisAdd
BLAST
Transmembranei132 – 15221HelicalSequence AnalysisAdd
BLAST
Transmembranei168 – 18821HelicalSequence AnalysisAdd
BLAST
Transmembranei201 – 22121HelicalSequence AnalysisAdd
BLAST
Transmembranei228 – 24821HelicalSequence AnalysisAdd
BLAST
Transmembranei279 – 29921HelicalSequence AnalysisAdd
BLAST
Transmembranei312 – 33221HelicalSequence AnalysisAdd
BLAST
Transmembranei344 – 36421HelicalSequence AnalysisAdd
BLAST
Transmembranei382 – 40221HelicalSequence AnalysisAdd
BLAST
Transmembranei423 – 44321HelicalSequence AnalysisAdd
BLAST
Transmembranei458 – 47821HelicalSequence AnalysisAdd
BLAST
Transmembranei512 – 53221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane respiratory chain complex IV Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Cytochrome c oxidase subunit 1 homolog, bacteroidPRO_0000183469Add
BLAST

Expressioni

Developmental stagei

Bacteroid (nitrogen-fixing endosymbiont).

Inductioni

By low oxygen levels.

Interactioni

Protein-protein interaction databases

STRINGi224911.blr2763.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3278.
HOGENOMiHOG000277908.
InParanoidiQ03073.
KOiK00404.
OrthoDBiEOG6G7QZX.
PhylomeDBiQ03073.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
IPR004677. Cyt_c_oxidase_cbb3_su1.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
SUPFAMiSSF81442. SSF81442. 1 hit.
TIGRFAMsiTIGR00780. ccoN. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPSISKSM TIGESGLAVV FAATAFLCVI AAAKALDAPF AFHAALSAAA
60 70 80 90 100
SVAAVFCIVN RYFERPAALP PAEINGRPNY NMGPIKFSSF MAMFWGIAGF
110 120 130 140 150
LVGLIIASQL AWPALNFDLP WISFGRLRPL HTSAVIFAFG GNVLIATSFY
160 170 180 190 200
VVQKSCRVRL AGDLAPWFVV VGYNFFILVA GTGYLLGVTQ SKEYAEPEWY
210 220 230 240 250
ADLWLTIVWV VYLLVFLATI IKRKEPHIFV ANWFYLAFIV TIAVLHLGNN
260 270 280 290 300
PALPVSAFGS KSYVAWGGIQ DAMFQWWYGH NAVGFFLTAG FLAIMYYFIP
310 320 330 340 350
KRAERPIYSY RLSIIHFWAL IFLYIWAGPH HLHYTALPDW TQTLGMTFSI
360 370 380 390 400
MLWMPSWGGM INGLMTLSGA WDKLRTDPVL RMLVVSVAFY GMSTFEGPMM
410 420 430 440 450
SIKVVNSLSH YTDWTIGHVH SGALGWVGFV SFGALYCLVP WAWNRKGLYS
460 470 480 490 500
LKLVNWHFWV ATLGIVLYIS AMWVSGILQG LMWRAYTSLG FLEYSFIETV
510 520 530 540
EAMHPFYIIR AAGGGLFLIG ALIMAYNLWM TVRVGEAEVQ MPVALQPAE
Length:549
Mass (Da):61,273
Last modified:February 1, 1996 - v1
Checksum:iEEFBB48F18C364E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07487 Genomic DNA. Translation: AAA26203.1.
AJ005001 Genomic DNA. Translation: CAA06279.1.
BA000040 Genomic DNA. Translation: BAC48028.1.
PIRiA47468.
RefSeqiNP_769403.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC48028; BAC48028; BAC48028.
GeneIDi1054103.
KEGGibja:blr2763.
PATRICi21188974. VBIBraJap65052_2745.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07487 Genomic DNA. Translation: AAA26203.1.
AJ005001 Genomic DNA. Translation: CAA06279.1.
BA000040 Genomic DNA. Translation: BAC48028.1.
PIRiA47468.
RefSeqiNP_769403.1. NC_004463.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.blr2763.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC48028; BAC48028; BAC48028.
GeneIDi1054103.
KEGGibja:blr2763.
PATRICi21188974. VBIBraJap65052_2745.

Phylogenomic databases

eggNOGiCOG3278.
HOGENOMiHOG000277908.
InParanoidiQ03073.
KOiK00404.
OrthoDBiEOG6G7QZX.
PhylomeDBiQ03073.

Enzyme and pathway databases

UniPathwayiUPA00705.
BioCyciBJAP224911:GJEJ-2787-MONOMER.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
IPR004677. Cyt_c_oxidase_cbb3_su1.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
SUPFAMiSSF81442. SSF81442. 1 hit.
TIGRFAMsiTIGR00780. ccoN. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genes for a microaerobically induced oxidase complex in Bradyrhizobium japonicum are essential for a nitrogen-fixing endosymbiosis."
    Preisig O., Anthamatten D., Hennecke H.
    Proc. Natl. Acad. Sci. U.S.A. 90:3309-3313(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: USDA 110spc4.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiFIXN_BRADU
AccessioniPrimary (citable) accession number: Q03073
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: January 7, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This cytochrome oxidase is probably a cbb3-type.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.