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Protein

Beta-chimaerin

Gene

Chn2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

GTPase-activating protein for p21-rac.

Enzyme regulationi

In the inactive state, the N terminus protrudes into the active site of the Rho-GAP domain, sterically blocking Rac binding. Phospholipid binding to the Phorbol-ester/DAG-type zinc-finger/C1 domain triggers the cooperative dissociation of these interactions, allowing the N-terminus to move out of the active site and thereby activating the enzyme (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri41 – 91Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

  • GTPase activator activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • phorbol ester receptor activity Source: RGD

GO - Biological processi

  • acrosome assembly Source: RGD
  • intracellular signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-chimaerin
Alternative name(s):
Beta-chimerin
Rho GTPase-activating protein 3
Gene namesi
Name:Chn2
Synonyms:Arhgap3, Bch
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620140. Chn2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000566991 – 295Beta-chimaerinAdd BLAST295

Proteomic databases

PaxDbiQ03070.

Expressioni

Tissue specificityi

Found in cerebellum and testis.

Developmental stagei

Expressed specifically in late stage spermatocytes. In the cerebellum, emergence of beta-2 isoform coincides with granule cells maturation and exhibits postnatal developmental increases. Expression is specifically reduced in weaver mutant.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012655.

Structurei

3D structure databases

ProteinModelPortaliQ03070.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 295Rho-GAPPROSITE-ProRule annotationAdd BLAST192

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri41 – 91Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

SH2 domain, Zinc-finger

Phylogenomic databases

eggNOGiKOG1453. Eukaryota.
ENOG410YM3I. LUCA.
HOGENOMiHOG000231926.
HOVERGENiHBG080489.
InParanoidiQ03070.
KOiK20630.

Family and domain databases

CDDicd00029. C1. 1 hit.
Gene3Di1.10.555.10. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR002219. PE/DAG-bd.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Beta-1 (identifier: Q03070-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLCTSPVNCL DSVCCTSINI SSLVRRAALT HNDNHFNYEK THNFKVHTFR
60 70 80 90 100
GPHWCEYCAN FMWGLIAQGV RCSDCGLNVH KQCSKHVPND CQPDLKRIKK
110 120 130 140 150
VYCCDLTTLV KAHNTQRPMV VDICIREIEA RGLKSEGLYR VSGFTEHIED
160 170 180 190 200
VKMAFDRDGE KADISANIYP DINIITGALK LYFRDLPIPI ITYDTYTKFI
210 220 230 240 250
EAAKISNADE RLEAVHEVLM LLPPAHYETL RYLMIHLKKV TMNEKDNLMN
260 270 280 290
AENLGIVFGP TLMRPPEDST LTTLHDMRYQ KLIVQILIEN EDVLF
Length:295
Mass (Da):33,837
Last modified:November 1, 1995 - v1
Checksum:iD7692D957B4816BD
GO
Isoform Beta-2 (identifier: Q03070-2)
Sequence is not available
Length:
Mass (Da):

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69489 mRNA. Translation: CAA49244.1.
L07494 mRNA. Translation: AAA40809.1.
PIRiA45485. S29956.
RefSeqiNP_114473.1. NM_032084.1. [Q03070-1]
UniGeneiRn.10521.

Genome annotation databases

GeneIDi84031.
KEGGirno:84031.
UCSCiRGD:620140. rat. [Q03070-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69489 mRNA. Translation: CAA49244.1.
L07494 mRNA. Translation: AAA40809.1.
PIRiA45485. S29956.
RefSeqiNP_114473.1. NM_032084.1. [Q03070-1]
UniGeneiRn.10521.

3D structure databases

ProteinModelPortaliQ03070.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012655.

Proteomic databases

PaxDbiQ03070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84031.
KEGGirno:84031.
UCSCiRGD:620140. rat. [Q03070-1]

Organism-specific databases

CTDi1124.
RGDi620140. Chn2.

Phylogenomic databases

eggNOGiKOG1453. Eukaryota.
ENOG410YM3I. LUCA.
HOGENOMiHOG000231926.
HOVERGENiHBG080489.
InParanoidiQ03070.
KOiK20630.

Miscellaneous databases

PROiQ03070.

Family and domain databases

CDDicd00029. C1. 1 hit.
Gene3Di1.10.555.10. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR002219. PE/DAG-bd.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHIO_RAT
AccessioniPrimary (citable) accession number: Q03070
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.