Reviewed, UniProtKB/Swiss-Prot Q03069 (DEGM_BACB2)

Names and origin

Protein names

Recommended name:
    Sensor protein degM
    EC=2.7.13.3

Gene names

Name:

degM

Organism

Bacillus sp. (strain B21-2)

Taxonomic identifier

69001 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in a sensory transduction pathway that enhances the production of minor proteases.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Sensor protein degM		PRO_0000074754
Regions
	Transmembrane	27 – 47	21	Potential
	Transmembrane	57 – 77	21	Potential
	Transmembrane	91 – 111	21	Potential
	Transmembrane	122 – 142	21	Potential
	Domain	170 – 370	201	Histidine kinase
	Compositional bias	306 – 315	10	Gly-rich
	Compositional bias	330 – 340	11	Gly-rich
Amino acid modifications
	Modified residue	173	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03069-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6494279E0879294B	FASTA	373	41,614
	10 20 30 40 50 60 MSGAVLLCLF FSINVGNIQW DLRYIPILLA FLYGGKRAGW GVAAIAVVGR IGQGGDLFLL 70 80 90 100 110 120 GVVLIVLTAL FYALCVNRFY MLPPRWSRIR YASLLVILPA TIQTFGTLYL INYENHLSES 130 140 150 160 170 180 WVAGWLYIVF LVVTVVLVTY LFETLLEKER IVAELVATEK DFTKGELAAS IAHEVRNPLT 190 200 210 220 230 240 VVKGFVQLLS EDKQHAEYHK LILSELDRAE SIIYEFLNTT RPQTNATFHL NETAREVVAL 250 260 270 280 290 300 LTPYAQERSI QLTIGTCEQA IVNGNENKVK QALMNFVKNG IEASNEGDTV TIQLDRLKDR 310 320 330 340 350 360 AQIEINDNGV GMSRQQLKQL GTAYFTTKES GNGIGTMVSI RIVEMMNGMV TFKSKPGKGT 370 KVVLSLPIEK ENE

References

[1]	"Cloning and nucleotide sequence of degM, the regulatory gene, degM, for minor protease in Bacillus subtilis." Masui A., Fujiwara N., Takagi M., Imanaka T. J. Ferment. Bioeng. 74:230-233(1992) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10690 Genomic DNA. Translation: BAA01532.1.
PIR	I39833.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Pfam	PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DEGM_BACB2

Accession

Primary (citable) accession number: Q03069

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)