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Protein

Choline O-acetyltransferase

Gene

Chat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei335Proton acceptorBy similarity1
Binding sitei451Coenzyme ABy similarity1
Binding sitei552Coenzyme ABy similarity1

GO - Molecular functioni

  • choline O-acetyltransferase activity Source: MGI

GO - Biological processi

  • adult walking behavior Source: MGI
  • chemical synaptic transmission Source: MGI
  • dendrite development Source: MGI
  • establishment of synaptic specificity at neuromuscular junction Source: MGI
  • muscle organ development Source: MGI
  • neuromuscular synaptic transmission Source: MGI
  • neuron differentiation Source: MGI
  • neurotransmitter biosynthetic process Source: UniProtKB-KW
  • rhythmic behavior Source: MGI
  • rhythmic excitation Source: MGI

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processNeurotransmitter biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase (EC:2.3.1.6)
Short name:
CHOACTase
Short name:
ChAT
Short name:
Choline acetylase
Gene namesi
Name:Chat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88392 Chat

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5900
GuidetoPHARMACOLOGYi2480

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101551 – 641Choline O-acetyltransferaseAdd BLAST641

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineBy similarity1
Modified residuei366PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ03059
PRIDEiQ03059

PTM databases

iPTMnetiQ03059
PhosphoSitePlusiQ03059

Expressioni

Gene expression databases

CleanExiMM_CHAT

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070865

Structurei

3D structure databases

ProteinModelPortaliQ03059
SMRiQ03059
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni413 – 425Coenzyme A bindingBy similarityAdd BLAST13

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3717 Eukaryota
ENOG410XNZ9 LUCA
HOVERGENiHBG107717
InParanoidiQ03059
PhylomeDBiQ03059

Family and domain databases

InterProiView protein in InterPro
IPR000542 Carn_acyl_trans
PANTHERiPTHR22589 PTHR22589, 1 hit
PfamiView protein in Pfam
PF00755 Carn_acyltransf, 1 hit
PROSITEiView protein in PROSITE
PS00439 ACYLTRANSF_C_1, 1 hit
PS00440 ACYLTRANSF_C_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q03059-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPILEKVPPK MPVQASSCEE VLDLPKLPVP PLQQTLATYL QCMQHLVPEE
60 70 80 90 100
QFRKSQAIVK RFGAPGGLGE TLQEKLLERQ EKTANWVSEY WLNDMYLNNR
110 120 130 140 150
LALPVNSSPA VIFARQHFQD TNDQLRFAAS LISGVLSYKA LLDSQSIPTD
160 170 180 190 200
WAKGQLSGQP LCMKQYYRLF SSYRLPGHTQ DTLVAQKSSI MPEPEHVIVA
210 220 230 240 250
CCNQFFVLDV VINFRRLSEG DLFTQLRKIV KMASNEDERL PPIGLLTSDG
260 270 280 290 300
RSEWAKARTV LLKDSTNRDS LDMIERCICL VCLDGPGTGD LSDTHRALQL
310 320 330 340 350
LHGGGCSLNG ANRWYDKSLQ FVVGRDGTCG VVCEHSPFDG IVLVQCTEHL
360 370 380 390 400
LKHMMTGNKK LVRVDSVSEL PAPRRLRWKC SPETQGHLAS SAEKLQRIVK
410 420 430 440 450
NLDFIVYKFD NYGKTFIKKQ KCSPDGFIQV ALQLAYYRLY QRLVPTYESA
460 470 480 490 500
SIRRFQEGRV DNIRSATPEA LAFVQAMTDH KAAVLASEKL QLLQRAIQAQ
510 520 530 540 550
TEYTVMAITG MAIDNHLLAL RELARDLCKE PPEMFMDETY LMSNRFILST
560 570 580 590 600
SQVPTTMEMF CCYGPVVPNG YGACYNPHAE AITFCISSFH GCKETSSVEF
610 620 630 640
AEAVGASLVD MRDLCSSRQP ADSKPPTAKE RARGPSQAKQ S
Length:641
Mass (Da):71,853
Last modified:January 23, 2007 - v2
Checksum:i9BE0010779C8AE6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12487 mRNA Translation: BAA02056.1
D12486 Genomic DNA Translation: BAA02055.1
D12488 mRNA Translation: BAA20976.1
D12489 mRNA Translation: BAA20977.1
D12490 mRNA Translation: BAA02057.1
D12491 mRNA Translation: BAA20978.1
D12492 mRNA Translation: BAA20979.1
D12493 mRNA Translation: BAA20980.1
PIRiB43777
UniGeneiMm.442817

Similar proteinsi

Entry informationi

Entry nameiCLAT_MOUSE
AccessioniPrimary (citable) accession number: Q03059
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health