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Q03059 (CLAT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Choline O-acetyltransferase

Short name=CHOACTase
Short name=ChAT
Short name=Choline acetylase
EC=2.3.1.6
Gene names
Name:Chat
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length641 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Catalytic activity

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sequence similarities

Belongs to the carnitine/choline acetyltransferase family.

Ontologies

Keywords
   Biological processNeurotransmitter biosynthesis
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadult walking behavior

Inferred from mutant phenotype PubMed 15820692. Source: MGI

dendrite development

Inferred from mutant phenotype PubMed 12441053. Source: MGI

establishment of synaptic specificity at neuromuscular junction

Inferred from mutant phenotype PubMed 12533614. Source: MGI

muscle organ development

Inferred from mutant phenotype PubMed 12441053. Source: MGI

neuromuscular synaptic transmission

Inferred from mutant phenotype PubMed 12441053. Source: MGI

neuron differentiation

Inferred from mutant phenotype PubMed 12441053. Source: MGI

neurotransmitter biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

rhythmic behavior

Inferred from mutant phenotype PubMed 15820692. Source: MGI

rhythmic excitation

Inferred from mutant phenotype PubMed 15820692. Source: MGI

synaptic transmission

Inferred from mutant phenotype PubMed 12533614. Source: MGI

   Cellular_componentaxon

Inferred from direct assay PubMed 19906978PubMed 20176735. Source: MGI

mitochondrion

Inferred from direct assay PubMed 18614015. Source: MGI

neuron projection

Inferred from direct assay PubMed 12115678. Source: MGI

neuronal cell body

Inferred from direct assay PubMed 9853118. Source: MGI

   Molecular_functioncholine O-acetyltransferase activity

Inferred from mutant phenotype PubMed 12441053PubMed 12533614. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 641640Choline O-acetyltransferase
PRO_0000210155

Regions

Region413 – 42513Coenzyme A binding By similarity

Sites

Active site3351Proton acceptor By similarity
Binding site4511Coenzyme A By similarity
Binding site5521Coenzyme A By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03059 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 9BE0010779C8AE6D

FASTA64171,853
        10         20         30         40         50         60 
MPILEKVPPK MPVQASSCEE VLDLPKLPVP PLQQTLATYL QCMQHLVPEE QFRKSQAIVK 

        70         80         90        100        110        120 
RFGAPGGLGE TLQEKLLERQ EKTANWVSEY WLNDMYLNNR LALPVNSSPA VIFARQHFQD 

       130        140        150        160        170        180 
TNDQLRFAAS LISGVLSYKA LLDSQSIPTD WAKGQLSGQP LCMKQYYRLF SSYRLPGHTQ 

       190        200        210        220        230        240 
DTLVAQKSSI MPEPEHVIVA CCNQFFVLDV VINFRRLSEG DLFTQLRKIV KMASNEDERL 

       250        260        270        280        290        300 
PPIGLLTSDG RSEWAKARTV LLKDSTNRDS LDMIERCICL VCLDGPGTGD LSDTHRALQL 

       310        320        330        340        350        360 
LHGGGCSLNG ANRWYDKSLQ FVVGRDGTCG VVCEHSPFDG IVLVQCTEHL LKHMMTGNKK 

       370        380        390        400        410        420 
LVRVDSVSEL PAPRRLRWKC SPETQGHLAS SAEKLQRIVK NLDFIVYKFD NYGKTFIKKQ 

       430        440        450        460        470        480 
KCSPDGFIQV ALQLAYYRLY QRLVPTYESA SIRRFQEGRV DNIRSATPEA LAFVQAMTDH 

       490        500        510        520        530        540 
KAAVLASEKL QLLQRAIQAQ TEYTVMAITG MAIDNHLLAL RELARDLCKE PPEMFMDETY 

       550        560        570        580        590        600 
LMSNRFILST SQVPTTMEMF CCYGPVVPNG YGACYNPHAE AITFCISSFH GCKETSSVEF 

       610        620        630        640 
AEAVGASLVD MRDLCSSRQP ADSKPPTAKE RARGPSQAKQ S 

« Hide

References

[1]"Complementary DNAs for choline acetyltransferase from spinal cords of rat and mouse: nucleotide sequences, expression in mammalian cells, and in situ hybridization."
Ishii K., Oda Y., Ichikawa T., Deguchi T.
Brain Res. Mol. Brain Res. 7:151-159(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Spinal cord.
[2]"Gene expression of mouse choline acetyltransferase. Alternative splicing and identification of a highly active promoter region."
Misawa H., Ishii K., Deguchi T.
J. Biol. Chem. 267:20392-20399(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 1-219.
Tissue: Spinal cord.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D12487 mRNA. Translation: BAA02056.1.
D12486 Genomic DNA. Translation: BAA02055.1.
D12488 mRNA. Translation: BAA20976.1.
D12489 mRNA. Translation: BAA20977.1.
D12490 mRNA. Translation: BAA02057.1.
D12491 mRNA. Translation: BAA20978.1.
D12492 mRNA. Translation: BAA20979.1.
D12493 mRNA. Translation: BAA20980.1.
PIRB43777.
UniGeneMm.442817.

3D structure databases

ProteinModelPortalQ03059.
SMRQ03059. Positions 22-617.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL5900.

PTM databases

PhosphoSiteQ03059.

Proteomic databases

PaxDbQ03059.
PRIDEQ03059.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:88392. Chat.

Phylogenomic databases

eggNOGNOG70127.
HOVERGENHBG107717.
InParanoidQ03059.
PhylomeDBQ03059.

Gene expression databases

CleanExMM_CHAT.
GenevestigatorQ03059.

Family and domain databases

InterProIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERPTHR22589. PTHR22589. 1 hit.
PfamPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ03059.
SOURCESearch...

Entry information

Entry nameCLAT_MOUSE
AccessionPrimary (citable) accession number: Q03059
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot