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Q03059

- CLAT_MOUSE

UniProt

Q03059 - CLAT_MOUSE

Protein

Choline O-acetyltransferase

Gene

Chat

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

    Catalytic activityi

    Acetyl-CoA + choline = CoA + O-acetylcholine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei335 – 3351Proton acceptorBy similarity
    Binding sitei451 – 4511Coenzyme ABy similarity
    Binding sitei552 – 5521Coenzyme ABy similarity

    GO - Molecular functioni

    1. choline O-acetyltransferase activity Source: MGI

    GO - Biological processi

    1. adult walking behavior Source: MGI
    2. dendrite development Source: MGI
    3. establishment of synaptic specificity at neuromuscular junction Source: MGI
    4. muscle organ development Source: MGI
    5. neuromuscular synaptic transmission Source: MGI
    6. neuron differentiation Source: MGI
    7. neurotransmitter biosynthetic process Source: UniProtKB-KW
    8. rhythmic behavior Source: MGI
    9. rhythmic excitation Source: MGI
    10. synaptic transmission Source: MGI

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Neurotransmitter biosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Choline O-acetyltransferase (EC:2.3.1.6)
    Short name:
    CHOACTase
    Short name:
    ChAT
    Short name:
    Choline acetylase
    Gene namesi
    Name:Chat
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:88392. Chat.

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: MGI
    2. mitochondrion Source: MGI
    3. neuronal cell body Source: MGI
    4. neuron projection Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 641640Choline O-acetyltransferasePRO_0000210155Add
    BLAST

    Proteomic databases

    PaxDbiQ03059.
    PRIDEiQ03059.

    PTM databases

    PhosphoSiteiQ03059.

    Expressioni

    Gene expression databases

    CleanExiMM_CHAT.
    GenevestigatoriQ03059.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03059.
    SMRiQ03059. Positions 22-617.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni413 – 42513Coenzyme A bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG70127.
    HOVERGENiHBG107717.
    InParanoidiQ03059.
    PhylomeDBiQ03059.

    Family and domain databases

    InterProiIPR000542. Carn_acyl_trans.
    [Graphical view]
    PANTHERiPTHR22589. PTHR22589. 1 hit.
    PfamiPF00755. Carn_acyltransf. 1 hit.
    [Graphical view]
    PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
    PS00440. ACYLTRANSF_C_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q03059-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPILEKVPPK MPVQASSCEE VLDLPKLPVP PLQQTLATYL QCMQHLVPEE    50
    QFRKSQAIVK RFGAPGGLGE TLQEKLLERQ EKTANWVSEY WLNDMYLNNR 100
    LALPVNSSPA VIFARQHFQD TNDQLRFAAS LISGVLSYKA LLDSQSIPTD 150
    WAKGQLSGQP LCMKQYYRLF SSYRLPGHTQ DTLVAQKSSI MPEPEHVIVA 200
    CCNQFFVLDV VINFRRLSEG DLFTQLRKIV KMASNEDERL PPIGLLTSDG 250
    RSEWAKARTV LLKDSTNRDS LDMIERCICL VCLDGPGTGD LSDTHRALQL 300
    LHGGGCSLNG ANRWYDKSLQ FVVGRDGTCG VVCEHSPFDG IVLVQCTEHL 350
    LKHMMTGNKK LVRVDSVSEL PAPRRLRWKC SPETQGHLAS SAEKLQRIVK 400
    NLDFIVYKFD NYGKTFIKKQ KCSPDGFIQV ALQLAYYRLY QRLVPTYESA 450
    SIRRFQEGRV DNIRSATPEA LAFVQAMTDH KAAVLASEKL QLLQRAIQAQ 500
    TEYTVMAITG MAIDNHLLAL RELARDLCKE PPEMFMDETY LMSNRFILST 550
    SQVPTTMEMF CCYGPVVPNG YGACYNPHAE AITFCISSFH GCKETSSVEF 600
    AEAVGASLVD MRDLCSSRQP ADSKPPTAKE RARGPSQAKQ S 641
    Length:641
    Mass (Da):71,853
    Last modified:January 23, 2007 - v2
    Checksum:i9BE0010779C8AE6D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D12487 mRNA. Translation: BAA02056.1.
    D12486 Genomic DNA. Translation: BAA02055.1.
    D12488 mRNA. Translation: BAA20976.1.
    D12489 mRNA. Translation: BAA20977.1.
    D12490 mRNA. Translation: BAA02057.1.
    D12491 mRNA. Translation: BAA20978.1.
    D12492 mRNA. Translation: BAA20979.1.
    D12493 mRNA. Translation: BAA20980.1.
    PIRiB43777.
    UniGeneiMm.442817.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D12487 mRNA. Translation: BAA02056.1 .
    D12486 Genomic DNA. Translation: BAA02055.1 .
    D12488 mRNA. Translation: BAA20976.1 .
    D12489 mRNA. Translation: BAA20977.1 .
    D12490 mRNA. Translation: BAA02057.1 .
    D12491 mRNA. Translation: BAA20978.1 .
    D12492 mRNA. Translation: BAA20979.1 .
    D12493 mRNA. Translation: BAA20980.1 .
    PIRi B43777.
    UniGenei Mm.442817.

    3D structure databases

    ProteinModelPortali Q03059.
    SMRi Q03059. Positions 22-617.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL5900.

    PTM databases

    PhosphoSitei Q03059.

    Proteomic databases

    PaxDbi Q03059.
    PRIDEi Q03059.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:88392. Chat.

    Phylogenomic databases

    eggNOGi NOG70127.
    HOVERGENi HBG107717.
    InParanoidi Q03059.
    PhylomeDBi Q03059.

    Miscellaneous databases

    PROi Q03059.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_CHAT.
    Genevestigatori Q03059.

    Family and domain databases

    InterProi IPR000542. Carn_acyl_trans.
    [Graphical view ]
    PANTHERi PTHR22589. PTHR22589. 1 hit.
    Pfami PF00755. Carn_acyltransf. 1 hit.
    [Graphical view ]
    PROSITEi PS00439. ACYLTRANSF_C_1. 1 hit.
    PS00440. ACYLTRANSF_C_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complementary DNAs for choline acetyltransferase from spinal cords of rat and mouse: nucleotide sequences, expression in mammalian cells, and in situ hybridization."
      Ishii K., Oda Y., Ichikawa T., Deguchi T.
      Brain Res. Mol. Brain Res. 7:151-159(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: Spinal cord.
    2. "Gene expression of mouse choline acetyltransferase. Alternative splicing and identification of a highly active promoter region."
      Misawa H., Ishii K., Deguchi T.
      J. Biol. Chem. 267:20392-20399(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 1-219.
      Tissue: Spinal cord.

    Entry informationi

    Entry nameiCLAT_MOUSE
    AccessioniPrimary (citable) accession number: Q03059
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3