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Protein

Choline O-acetyltransferase

Gene

Chat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Catalytic activityi

Acetyl-CoA + choline = CoA + O-acetylcholine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei335 – 3351Proton acceptorBy similarity
Binding sitei451 – 4511Coenzyme ABy similarity
Binding sitei552 – 5521Coenzyme ABy similarity

GO - Molecular functioni

  • choline O-acetyltransferase activity Source: MGI

GO - Biological processi

  • adult walking behavior Source: MGI
  • dendrite development Source: MGI
  • establishment of synaptic specificity at neuromuscular junction Source: MGI
  • muscle organ development Source: MGI
  • neuromuscular synaptic transmission Source: MGI
  • neuron differentiation Source: MGI
  • neurotransmitter biosynthetic process Source: UniProtKB-KW
  • rhythmic behavior Source: MGI
  • rhythmic excitation Source: MGI
  • synaptic transmission Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Neurotransmitter biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Choline O-acetyltransferase (EC:2.3.1.6)
Short name:
CHOACTase
Short name:
ChAT
Short name:
Choline acetylase
Gene namesi
Name:Chat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88392. Chat.

Subcellular locationi

GO - Cellular componenti

  • axon Source: MGI
  • cytoplasm Source: MGI
  • mitochondrion Source: MGI
  • neuronal cell body Source: MGI
  • neuron projection Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 641640Choline O-acetyltransferasePRO_0000210155Add
BLAST

Proteomic databases

PaxDbiQ03059.
PRIDEiQ03059.

PTM databases

PhosphoSiteiQ03059.

Expressioni

Gene expression databases

CleanExiMM_CHAT.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070865.

Structurei

3D structure databases

ProteinModelPortaliQ03059.
SMRiQ03059. Positions 22-617.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni413 – 42513Coenzyme A bindingBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG70127.
HOVERGENiHBG107717.
InParanoidiQ03059.
PhylomeDBiQ03059.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03059-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPILEKVPPK MPVQASSCEE VLDLPKLPVP PLQQTLATYL QCMQHLVPEE
60 70 80 90 100
QFRKSQAIVK RFGAPGGLGE TLQEKLLERQ EKTANWVSEY WLNDMYLNNR
110 120 130 140 150
LALPVNSSPA VIFARQHFQD TNDQLRFAAS LISGVLSYKA LLDSQSIPTD
160 170 180 190 200
WAKGQLSGQP LCMKQYYRLF SSYRLPGHTQ DTLVAQKSSI MPEPEHVIVA
210 220 230 240 250
CCNQFFVLDV VINFRRLSEG DLFTQLRKIV KMASNEDERL PPIGLLTSDG
260 270 280 290 300
RSEWAKARTV LLKDSTNRDS LDMIERCICL VCLDGPGTGD LSDTHRALQL
310 320 330 340 350
LHGGGCSLNG ANRWYDKSLQ FVVGRDGTCG VVCEHSPFDG IVLVQCTEHL
360 370 380 390 400
LKHMMTGNKK LVRVDSVSEL PAPRRLRWKC SPETQGHLAS SAEKLQRIVK
410 420 430 440 450
NLDFIVYKFD NYGKTFIKKQ KCSPDGFIQV ALQLAYYRLY QRLVPTYESA
460 470 480 490 500
SIRRFQEGRV DNIRSATPEA LAFVQAMTDH KAAVLASEKL QLLQRAIQAQ
510 520 530 540 550
TEYTVMAITG MAIDNHLLAL RELARDLCKE PPEMFMDETY LMSNRFILST
560 570 580 590 600
SQVPTTMEMF CCYGPVVPNG YGACYNPHAE AITFCISSFH GCKETSSVEF
610 620 630 640
AEAVGASLVD MRDLCSSRQP ADSKPPTAKE RARGPSQAKQ S
Length:641
Mass (Da):71,853
Last modified:January 23, 2007 - v2
Checksum:i9BE0010779C8AE6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12487 mRNA. Translation: BAA02056.1.
D12486 Genomic DNA. Translation: BAA02055.1.
D12488 mRNA. Translation: BAA20976.1.
D12489 mRNA. Translation: BAA20977.1.
D12490 mRNA. Translation: BAA02057.1.
D12491 mRNA. Translation: BAA20978.1.
D12492 mRNA. Translation: BAA20979.1.
D12493 mRNA. Translation: BAA20980.1.
PIRiB43777.
UniGeneiMm.442817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12487 mRNA. Translation: BAA02056.1.
D12486 Genomic DNA. Translation: BAA02055.1.
D12488 mRNA. Translation: BAA20976.1.
D12489 mRNA. Translation: BAA20977.1.
D12490 mRNA. Translation: BAA02057.1.
D12491 mRNA. Translation: BAA20978.1.
D12492 mRNA. Translation: BAA20979.1.
D12493 mRNA. Translation: BAA20980.1.
PIRiB43777.
UniGeneiMm.442817.

3D structure databases

ProteinModelPortaliQ03059.
SMRiQ03059. Positions 22-617.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070865.

Chemistry

ChEMBLiCHEMBL5900.

PTM databases

PhosphoSiteiQ03059.

Proteomic databases

PaxDbiQ03059.
PRIDEiQ03059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:88392. Chat.

Phylogenomic databases

eggNOGiNOG70127.
HOVERGENiHBG107717.
InParanoidiQ03059.
PhylomeDBiQ03059.

Miscellaneous databases

PROiQ03059.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CHAT.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complementary DNAs for choline acetyltransferase from spinal cords of rat and mouse: nucleotide sequences, expression in mammalian cells, and in situ hybridization."
    Ishii K., Oda Y., Ichikawa T., Deguchi T.
    Brain Res. Mol. Brain Res. 7:151-159(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Spinal cord.
  2. "Gene expression of mouse choline acetyltransferase. Alternative splicing and identification of a highly active promoter region."
    Misawa H., Ishii K., Deguchi T.
    J. Biol. Chem. 267:20392-20399(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 1-219.
    Tissue: Spinal cord.

Entry informationi

Entry nameiCLAT_MOUSE
AccessioniPrimary (citable) accession number: Q03059
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.