Q03058 (CAT_STRAG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloramphenicol acetyltransferase
Short name=CAT
EC=2.3.1.28

Gene names

Name:

cat

Encoded on

Plasmid pIP501

Organism

Streptococcus agalactiae

Taxonomic identifier

1311 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is an effector of chloramphenicol resistance in bacteria.
Catalytic activity	Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate.
Subunit structure	Homotrimer.
Sequence similarities	Belongs to the chloramphenicol acetyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Acyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chloramphenicol O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	Chloramphenicol acetyltransferase		PRO_0000165879
Sites
	Active site	189	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03058 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 7FD30D9B612BB7B9	FASTA	215	25,789
	10 20 30 40 50 60 MTFNIIELEN WDRKEYFEHY FNQQTTYSIT KEIDITLFKD MIKKKGYEIY PSLIYAIMEV 70 80 90 100 110 120 VNKNKVFRTG INSENKLGYW DKLNPLYTVF NKQTEKFTNI WTESDKNFIS FYNNYKNDLL 130 140 150 160 170 180 EYKDKEEMFP KKPIPENTIP ISMIPWIDFS SFNLNIGNNS SFLLPIITIG KFYSENNKIY 190 200 210 IPVALQLHHS VCDGYHASLF MNEFQDIIHR VDDWI

References

[1]	"Nucleotide sequence of the chloramphenicol resistance determinant of the streptococcal plasmid pIP501." Trieu-Cuot P., de Cespedes G., Horaud T. Plasmid 28:272-276(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X65462 Genomic DNA. Translation: CAA46455.1.
PIR	JQ1951.
RefSeq	NP_044466.1. NC_001797.1.
3D structure databases
ProteinModelPortal	Q03058.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1113365.
Phylogenomic databases
ProtClustDB	CLSK862213.
Family and domain databases
Gene3D	3.30.559.10. 1 hit.
InterPro	IPR023213. CAT-like_dom. IPR018372. Chloramphenicol_AcTrfase_AS. IPR001707. Cmp_AcTrfase. [Graphical view]
Pfam	PF00302. CAT. 1 hit. [Graphical view]
PIRSF	PIRSF000440. CAT. 1 hit.
ProDom	PD002660. Cmp_AcTrfase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM01059. CAT. 1 hit. [Graphical view]
PROSITE	PS00100. CAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAT_STRAG

Accession

Primary (citable) accession number: Q03058

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program