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Q03055

- MTV1_VIBS3

UniProt

Q03055 - MTV1_VIBS3

Protein

Modification methylase VspI

Gene

vspIM

Organism
Vibrio sp. (strain 343)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence ATTAAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the VspI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3525. M.VspI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase VspI (EC:2.1.1.72)
    Short name:
    M.VspI
    Alternative name(s):
    Adenine-specific methyltransferase VspI
    Gene namesi
    Name:vspIM
    OrganismiVibrio sp. (strain 343)
    Taxonomic identifieri29496 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 408408Modification methylase VspIPRO_0000087982Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ03055.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02384. N6_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03055-1 [UniParc]FASTAAdd to Basket

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    MKQSALFEAD DVEAISIDDV AFSLNVSSAS VRNWIKTGYL HKATKNSVTA    50
    ESFVAFKDEI LGTEKLNQRA NKSLKDQHDH SGLEEMIHNI IRSNEVHPEG 100
    LSDIYEESLS ESYKNKEGVF YTPKEIAADF FDYLPKDCSE LTFCDPCCGT 150
    GNFLIEAVKR GFKPCNIYGY DIDEVALEIS RSRLKELCGV AESNIEKRDF 200
    LSASYQIEQK YDVIFTNPPW GKKLPKKDKD SLADSLATGN SKDTSAIFFF 250
    ASMKILNSSG YLGFLLQDAF FNIASYESVR KAALANQIVA LIDFGKPFKG 300
    LLTKAKGIIL RKQCPDDQHA TICVSGNTKN EVSQRVFEKN PKSIFNFTCS 350
    ELDLEVVEHI LSIPHKTLRG SARWGLGIVT GNNKKFCLPE ARGGYIPVYK 400
    GSDITRKG 408
    Length:408
    Mass (Da):45,507
    Last modified:July 1, 1993 - v1
    Checksum:i31283121D15299A7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68658 Genomic DNA. Translation: CAA48625.1.
    PIRiS33683.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68658 Genomic DNA. Translation: CAA48625.1 .
    PIRi S33683.

    3D structure databases

    ProteinModelPortali Q03055.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3525. M.VspI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02384. N6_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vspl methylase belongs to m6A-gamma class of adenine methylases."
      Degtyarev S.K., Prikhod'Ko E.A., Prikhod'Ko G.G., Krasnykh V.N.
      Nucleic Acids Res. 21:2015-2015(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiMTV1_VIBS3
    AccessioniPrimary (citable) accession number: Q03055
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3