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Q03055 (MTV1_VIBS3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase VspI

Short name=M.VspI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase VspI
Gene names
Name:vspIM
OrganismVibrio sp. (strain 343)
Taxonomic identifier29496 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence ATTAAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the VspI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 408408Modification methylase VspI
PRO_0000087982

Sequences

Sequence LengthMass (Da)Tools
Q03055 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 31283121D15299A7

FASTA40845,507
        10         20         30         40         50         60 
MKQSALFEAD DVEAISIDDV AFSLNVSSAS VRNWIKTGYL HKATKNSVTA ESFVAFKDEI 

        70         80         90        100        110        120 
LGTEKLNQRA NKSLKDQHDH SGLEEMIHNI IRSNEVHPEG LSDIYEESLS ESYKNKEGVF 

       130        140        150        160        170        180 
YTPKEIAADF FDYLPKDCSE LTFCDPCCGT GNFLIEAVKR GFKPCNIYGY DIDEVALEIS 

       190        200        210        220        230        240 
RSRLKELCGV AESNIEKRDF LSASYQIEQK YDVIFTNPPW GKKLPKKDKD SLADSLATGN 

       250        260        270        280        290        300 
SKDTSAIFFF ASMKILNSSG YLGFLLQDAF FNIASYESVR KAALANQIVA LIDFGKPFKG 

       310        320        330        340        350        360 
LLTKAKGIIL RKQCPDDQHA TICVSGNTKN EVSQRVFEKN PKSIFNFTCS ELDLEVVEHI 

       370        380        390        400 
LSIPHKTLRG SARWGLGIVT GNNKKFCLPE ARGGYIPVYK GSDITRKG 

« Hide

References

[1]"Vspl methylase belongs to m6A-gamma class of adenine methylases."
Degtyarev S.K., Prikhod'Ko E.A., Prikhod'Ko G.G., Krasnykh V.N.
Nucleic Acids Res. 21:2015-2015(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Biochemical characterization of VspI methyltransferase."
Degtyarev S.K., Prikhod'Ko E.A., Rechkunova N.I., Prikhod'Ko G.G., Krasnykh V.N.
Gene 157:65-66(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68658 Genomic DNA. Translation: CAA48625.1.
PIRS33683.

3D structure databases

ProteinModelPortalQ03055.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3525. M.VspI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
[Graphical view]
PfamPF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTV1_VIBS3
AccessionPrimary (citable) accession number: Q03055
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries