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Protein

Modification methylase VspI

Gene

vspIM

Organism
Vibrio sp. (strain 343)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence ATTAAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the VspI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3525 M.VspI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase VspI (EC:2.1.1.72)
Short name:
M.VspI
Alternative name(s):
Adenine-specific methyltransferase VspI
Gene namesi
Name:vspIM
OrganismiVibrio sp. (strain 343)
Taxonomic identifieri29496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879821 – 408Modification methylase VspIAdd BLAST408

Proteomic databases

PRIDEiQ03055

Structurei

3D structure databases

ProteinModelPortaliQ03055
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR003356 DNA_methylase_A-5
IPR002052 DNA_methylase_N6_adenine_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF02384 N6_Mtase, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS00092 N6_MTASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q03055-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQSALFEAD DVEAISIDDV AFSLNVSSAS VRNWIKTGYL HKATKNSVTA
60 70 80 90 100
ESFVAFKDEI LGTEKLNQRA NKSLKDQHDH SGLEEMIHNI IRSNEVHPEG
110 120 130 140 150
LSDIYEESLS ESYKNKEGVF YTPKEIAADF FDYLPKDCSE LTFCDPCCGT
160 170 180 190 200
GNFLIEAVKR GFKPCNIYGY DIDEVALEIS RSRLKELCGV AESNIEKRDF
210 220 230 240 250
LSASYQIEQK YDVIFTNPPW GKKLPKKDKD SLADSLATGN SKDTSAIFFF
260 270 280 290 300
ASMKILNSSG YLGFLLQDAF FNIASYESVR KAALANQIVA LIDFGKPFKG
310 320 330 340 350
LLTKAKGIIL RKQCPDDQHA TICVSGNTKN EVSQRVFEKN PKSIFNFTCS
360 370 380 390 400
ELDLEVVEHI LSIPHKTLRG SARWGLGIVT GNNKKFCLPE ARGGYIPVYK

GSDITRKG
Length:408
Mass (Da):45,507
Last modified:July 1, 1993 - v1
Checksum:i31283121D15299A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68658 Genomic DNA Translation: CAA48625.1
PIRiS33683

Entry informationi

Entry nameiMTV1_VIBS3
AccessioniPrimary (citable) accession number: Q03055
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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