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Protein

Modification methylase VspI

Gene

vspIM

Organism
Vibrio sp. (strain 343)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence ATTAAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the VspI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3525. M.VspI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase VspI (EC:2.1.1.72)
Short name:
M.VspI
Alternative name(s):
Adenine-specific methyltransferase VspI
Gene namesi
Name:vspIM
OrganismiVibrio sp. (strain 343)
Taxonomic identifieri29496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408Modification methylase VspIPRO_0000087982Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03055-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQSALFEAD DVEAISIDDV AFSLNVSSAS VRNWIKTGYL HKATKNSVTA
60 70 80 90 100
ESFVAFKDEI LGTEKLNQRA NKSLKDQHDH SGLEEMIHNI IRSNEVHPEG
110 120 130 140 150
LSDIYEESLS ESYKNKEGVF YTPKEIAADF FDYLPKDCSE LTFCDPCCGT
160 170 180 190 200
GNFLIEAVKR GFKPCNIYGY DIDEVALEIS RSRLKELCGV AESNIEKRDF
210 220 230 240 250
LSASYQIEQK YDVIFTNPPW GKKLPKKDKD SLADSLATGN SKDTSAIFFF
260 270 280 290 300
ASMKILNSSG YLGFLLQDAF FNIASYESVR KAALANQIVA LIDFGKPFKG
310 320 330 340 350
LLTKAKGIIL RKQCPDDQHA TICVSGNTKN EVSQRVFEKN PKSIFNFTCS
360 370 380 390 400
ELDLEVVEHI LSIPHKTLRG SARWGLGIVT GNNKKFCLPE ARGGYIPVYK

GSDITRKG
Length:408
Mass (Da):45,507
Last modified:July 1, 1993 - v1
Checksum:i31283121D15299A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68658 Genomic DNA. Translation: CAA48625.1.
PIRiS33683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68658 Genomic DNA. Translation: CAA48625.1.
PIRiS33683.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3525. M.VspI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Vspl methylase belongs to m6A-gamma class of adenine methylases."
    Degtyarev S.K., Prikhod'Ko E.A., Prikhod'Ko G.G., Krasnykh V.N.
    Nucleic Acids Res. 21:2015-2015(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiMTV1_VIBS3
AccessioniPrimary (citable) accession number: Q03055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 1, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.