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Q03055

- MTV1_VIBS3

UniProt

Q03055 - MTV1_VIBS3

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Protein

Modification methylase VspI

Gene
vspIM
Organism
Vibrio sp. (strain 343)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence ATTAAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the VspI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3525. M.VspI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase VspI (EC:2.1.1.72)
Short name:
M.VspI
Alternative name(s):
Adenine-specific methyltransferase VspI
Gene namesi
Name:vspIM
OrganismiVibrio sp. (strain 343)
Taxonomic identifieri29496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408Modification methylase VspIPRO_0000087982Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ03055.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03055-1 [UniParc]FASTAAdd to Basket

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MKQSALFEAD DVEAISIDDV AFSLNVSSAS VRNWIKTGYL HKATKNSVTA    50
ESFVAFKDEI LGTEKLNQRA NKSLKDQHDH SGLEEMIHNI IRSNEVHPEG 100
LSDIYEESLS ESYKNKEGVF YTPKEIAADF FDYLPKDCSE LTFCDPCCGT 150
GNFLIEAVKR GFKPCNIYGY DIDEVALEIS RSRLKELCGV AESNIEKRDF 200
LSASYQIEQK YDVIFTNPPW GKKLPKKDKD SLADSLATGN SKDTSAIFFF 250
ASMKILNSSG YLGFLLQDAF FNIASYESVR KAALANQIVA LIDFGKPFKG 300
LLTKAKGIIL RKQCPDDQHA TICVSGNTKN EVSQRVFEKN PKSIFNFTCS 350
ELDLEVVEHI LSIPHKTLRG SARWGLGIVT GNNKKFCLPE ARGGYIPVYK 400
GSDITRKG 408
Length:408
Mass (Da):45,507
Last modified:July 1, 1993 - v1
Checksum:i31283121D15299A7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68658 Genomic DNA. Translation: CAA48625.1.
PIRiS33683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68658 Genomic DNA. Translation: CAA48625.1 .
PIRi S33683.

3D structure databases

ProteinModelPortali Q03055.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3525. M.VspI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02384. N6_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Vspl methylase belongs to m6A-gamma class of adenine methylases."
    Degtyarev S.K., Prikhod'Ko E.A., Prikhod'Ko G.G., Krasnykh V.N.
    Nucleic Acids Res. 21:2015-2015(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiMTV1_VIBS3
AccessioniPrimary (citable) accession number: Q03055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3