Q03052 (PO3F1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
POU domain, class 3, transcription factor 1

Alternative name(s):
Octamer-binding protein 6
Short name=Oct-6
Octamer-binding transcription factor 6
Short name=OTF-6
POU domain transcription factor SCIP

Gene names

Name:	POU3F1
Synonyms:	OCT6, OTF6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis.
Subcellular location	Nucleus.
Tissue specificity	Expressed in embryonal stem cells and in the developing brain.
Sequence similarities	Belongs to the POU transcription factor family. Class-3 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon ensheathment Traceable author statement PubMed 8662541. Source: ProtInc forebrain development Inferred from electronic annotation. Source: Compara keratinocyte differentiation Inferred from electronic annotation. Source: Compara myelination in peripheral nervous system Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB transcription factor complex Inferred from electronic annotation. Source: Compara
Molecular_function	RNA polymerase II transcription coactivator activity Inferred from electronic annotation. Source: Compara sequence-specific DNA binding Inferred from direct assay PubMed 9242494. Source: MGI sequence-specific DNA binding transcription factor activity Traceable author statement Ref.4PubMed 8662541. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	POU domain, class 3, transcription factor 1		PRO_0000100720
Regions
	Domain	247 – 321	75	POU-specific
	DNA binding	339 – 398	60	Homeobox
	Compositional bias	27 – 45	19	Ala-rich
	Compositional bias	59 – 242	184	Gly-rich
	Compositional bias	225 – 228	4	Poly-Ala
	Compositional bias	418 – 423	6	Poly-Gly
	Compositional bias	430 – 435	6	Poly-Pro
	Compositional bias	439 – 444	6	Poly-His
Experimental info
	Sequence conflict	27 – 28	2	Missing in AAA59965. Ref.1
	Sequence conflict	87 – 88	2	GP → A in AAA59965. Ref.1
	Sequence conflict	87 – 88	2	GP → A in CAA79158. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q03052 [UniParc]. Last modified December 16, 2008. Version 3. Checksum: 563217BD6A3D00CD	FASTA	451	45,496
	10 20 30 40 50 60 MATTAQYLPR GPGGGAGGTG PLMHPDAAAA AAAAAAAERL HAGAAYREVQ KLMHHEWLGA 70 80 90 100 110 120 GAGHPVGLAH PQWLPTGGGG GGDWAGGPHL EHGKAGGGGT GRADDGGGGG GFHARLVHQG 130 140 150 160 170 180 AAHAGAAWAQ GSTAHHLGPA MSPSPGASGG HQPQPLGLYA QAAYPGGGGG GLAGMLAAGG 190 200 210 220 230 240 GGAGPGLHHA LHEDGHEAQL EPSPPPHLGA HGHAHGHAHA GGLHAAAAHL HPGAGGGGSS 250 260 270 280 290 300 VGEHSDEDAP SSDDLEQFAK QFKQRRIKLG FTQADVGLAL GTLYGNVFSQ TTICRFEALQ 310 320 330 340 350 360 LSFKNMCKLK PLLNKWLEET DSSSGSPTNL DKIAAQGRKR KKRTSIEVGV KGALESHFLK 370 380 390 400 410 420 CPKPSAHEIT GLADSLQLEK EVVRVWFCNR RQKEKRMTPA AGAGHPPMDD VYAPGELGPG 430 440 450 GGGASPPSAP PPPPPAALHH HHHHTLPGSV Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Oct-6: a regulator of keratinocyte gene expression in stratified squamous epithelia." Faus I., Hsu H.J., Fuchs E. Mol. Cell. Biol. 14:3263-3275(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The human Oct-6 POU transcription factor lacks the first 50 amino acids of its murine counterpart." Tobler A., Schreiber E., Fontana A. Nucleic Acids Res. 21:1043-1043(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-451.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L26494 mRNA. Translation: AAA59965.1. AL139158 Genomic DNA. Translation: CAI23136.1. CH471059 Genomic DNA. Translation: EAX07298.1. Z18284 mRNA. Translation: CAA79158.1.
IPI	IPI00008840.
PIR	A56018.
RefSeq	NP_002690.3. NM_002699.3.
UniGene	Hs.1837.
3D structure databases
ProteinModelPortal	Q03052.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-277N.
STRING	9606.ENSP00000362103.
Polymorphism databases
DMDM	218512119.
Proteomic databases
PaxDb	Q03052.
PRIDE	Q03052.
Protocols and materials databases
DNASU	5453.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000373012; ENSP00000362103; ENSG00000185668.
GeneID	5453.
KEGG	hsa:5453.
UCSC	uc001ccp.1. human.
Organism-specific databases
CTD	5453.
GeneCards	GC01M038509.
H-InvDB	HIX0028895.
HGNC	HGNC:9214. POU3F1.
MIM	602479. gene.
neXtProt	NX_Q03052.
PharmGKB	PA33538.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG261729.
HOGENOM	HOG000116303.
HOVERGEN	HBG053120.
InParanoid	Q03052.
KO	K09365.
OMA	MHHALHE.
OrthoDB	EOG4SQWXT.
PhylomeDB	Q03052.
Gene expression databases
Bgee	Q03052.
CleanEx	HS_POU3F1.
Genevestigator	Q03052.
GermOnline	ENSG00000185668. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.60. 1 hit. 1.10.260.40. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR010982. Lambda_DNA-bd_dom. IPR013847. POU. IPR000327. POU_specific. IPR016362. Transcription_factor_POU. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit. [Graphical view]
PIRSF	PIRSF002629. Transcription_factor_POU. 1 hit.
PRINTS	PR00028. POUDOMAIN.
SMART	SM00389. HOX. 1 hit. SM00352. POU. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit. SSF47413. Lambda_like_DNA. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	5453.
NextBio	21105.
SOURCE	Search...

Entry information

Entry name

PO3F1_HUMAN

Accession

Primary (citable) accession number: Q03052
Secondary accession number(s): Q5TAG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	December 16, 2008
Last modified:	May 1, 2013
This is version 130 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.