Reviewed, UniProtKB/Swiss-Prot Q03047 (CEFE_NOCLA)

Names and origin

Protein names

Recommended name:
    Deacetoxycephalosporin C synthetase
      Short name=DAOCS
    EC=1.14.20.1
Alternative name(s):
    Expandase

Gene names

Name:

cefE

Organism

Nocardia lactamdurans

Taxonomic identifier

1913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.
Catalytic activity	Penicillin N + 2-oxoglutarate + O2 = deacetoxycephalosporin C + succinate + CO2 + H2O.
Cofactor	Iron. Ascorbate.
Pathway	Antibiotic biosynthesis; cephalosporin C biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Iron Vitamin C
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW deacetoxycephalosporin-C synthase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Deacetoxycephalosporin C synthetase		PRO_0000219510

Sequences

	Sequence		Length	Mass (Da)
	Q03047-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: D007AD3DE67A1BDF	FASTA	314	34,530
	10 20 30 40 50 60 MTDATVPTFD LAELREGLHQ EEFRHCLREK GVFYLKGTGL PAEADHASGR EIAVDFFDHG 70 80 90 100 110 120 TEAEKKAVMT PIPTIRRGYA GLESESTAQI TNTGKYTDYS MSYSMGTADN LFPSAEFEKA 130 140 150 160 170 180 WEDYFARMYR ASQDVARQVL TSVGAEPEVG MDAFLDCEPL LRLRYFPEVP EDRVAEEQPL 190 200 210 220 230 240 RMAPHYDLSI VTLIHQTPCA NGFVSLQVEV DGSYVDIPAQ PGAVLVFCGA VATLVADGAI 250 260 270 280 290 300 KAPKHHVAAP GADKRVGSSR TSSVFFLRPN GDFRFSVPRA RECGFDVSIP AETATFDDWI 310 GGNYINIRKT AAAR

References

[1]

"Characterization and expression in Streptomyces lividans of cefD and cefE genes from Nocardia lactamdurans: the organization of the cephamycin gene cluster differs from that in Streptomyces clavuligerus." Coque J.J.R., Martin J.F., Liras P. Mol. Gen. Genet. 236:453-458(1993) [PubMed: 8437592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z13974 Genomic DNA. Translation: CAA78376.1.
PIR	S30900.
3D structure databases
HSSP	HSSP built from PDB template 1E5I based on UniProtKB P18548.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.20.1. 261149.
Family and domain databases
InterPro	IPR002057. Isopenicillin-N_synth_CS. IPR005123. Oxoglutarate/Fe-dep_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PROSITE	PS00185. IPNS_1. False negative. PS00186. IPNS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CEFE_NOCLA

Accession

Primary (citable) accession number: Q03047

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 5, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)