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Q03045

- AMYG_AMORE

UniProt

Q03045 - AMYG_AMORE

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Protein
Glucoamylase P
Gene
GAMP
Organism
Amorphotheca resinae (Creosote fungus) (Hormoconis resinae)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei149 – 1491Substrate By similarity
Active sitei205 – 2051Proton acceptor By similarity
Active sitei208 – 2081Proton donor By similarity

GO - Molecular functioni

  1. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC
  2. starch binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH15. Glycoside Hydrolase Family 15.
mycoCLAPiGLA15P_HORRE.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase P (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:GAMP
OrganismiAmorphotheca resinae (Creosote fungus) (Hormoconis resinae)
Taxonomic identifieri5101 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesLeotiomycetes incertae sedisMyxotrichaceaeAmorphotheca

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929
Add
BLAST
Chaini30 – 616587Glucoamylase P
PRO_0000001471Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi200 – 2001N-linked (GlcNAc...) Reviewed prediction
Glycosylationi427 – 4271N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ03045.
SMRiQ03045. Positions 30-503.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini501 – 608108CBM20
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR000165. Glucoamylase.
IPR008291. Glucoamylase_SBD.
IPR011613. Glyco_hydro_15.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00686. CBM_20. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PIRSFiPIRSF001031. Glu-a-glcsd_SBD. 1 hit.
PRINTSiPR00736. GLHYDRLASE15.
SMARTiSM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49452. SSF49452. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03045-1 [UniParc]FASTAAdd to Basket

« Hide

MRLLPSSCAG ALSLLCSLAI AAPTELKARD LSSFIASERA IALQGALNNI    50
GPDGSAVPGA GAGFVVASPS KANPDYFYTW SRDSALTLKM IIDEFILGNT 100
TLQTIIEQYI HAQAVLQTVS NPSGTFLPDG VGLGEPKFMV DGTRFNGPWG 150
RPQRDGPALR AIALMTYSNW LIKNGQFAEA KTKIWPIIAN DLSYVGQYWN 200
QSGFDLWEET YASSFFTIQN QHRALVEGAQ LAHDLGVTCT GCDQAPEVLC 250
FLQSFWNGKY IVSNINVNNG RTGLDGNSIL GAISTFDIDA YCDSPTLQPC 300
HSQSLANFKV LTDTFRNLYT INAGIPEGQG VAVGRYAEDV YMGGNPWYLI 350
TTAAAEFLYD AVAQWKARHV LTVDETSLAF FKDIYPEVTV REYKSGNANS 400
PFAQIMDAVT AYADSYVAIA EKYIPSNGSL SEQFNRDTGT PLSAIDLTWS 450
YAAFITMSQR RAGQYPSSWG SRNALPPPTT CSASSTPGIY TPATAAGAPN 500
VTSSCQVSIT FNINATTYYG ENLYVIGNSS DLGAWNIADA YPLSASAYTQ 550
DRPLWSAAIP LNAGEVISYQ YVRQEDCDQP YIYETVNRTL TVPACGGAAV 600
TTDDAWMGPV GSSGNC 616
Length:616
Mass (Da):66,432
Last modified:February 1, 1994 - v1
Checksum:iB5F4DC8EEBB152FB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68143 Genomic DNA. Translation: CAA48243.1.
X67708 mRNA. Translation: CAA47945.1.
PIRiS33908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68143 Genomic DNA. Translation: CAA48243.1 .
X67708 mRNA. Translation: CAA47945.1 .
PIRi S33908.

3D structure databases

ProteinModelPortali Q03045.
SMRi Q03045. Positions 30-503.
ModBasei Search...

Protein family/group databases

CAZyi CBM20. Carbohydrate-Binding Module Family 20.
GH15. Glycoside Hydrolase Family 15.
mycoCLAPi GLA15P_HORRE.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR000165. Glucoamylase.
IPR008291. Glucoamylase_SBD.
IPR011613. Glyco_hydro_15.
IPR013783. Ig-like_fold.
[Graphical view ]
Pfami PF00686. CBM_20. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view ]
PIRSFi PIRSF001031. Glu-a-glcsd_SBD. 1 hit.
PRINTSi PR00736. GLHYDRLASE15.
SMARTi SM01065. CBM_2. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
SSF49452. SSF49452. 1 hit.
PROSITEi PS51166. CBM20. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Glucoamylase P gene of Hormoconis resinae: molecular cloning, sequencing and introduction into Trichoderma reesei."
    Joutsjoki V.V., Torkkeli T.K.
    FEMS Microbiol. Lett. 78:237-243(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 20495 / CBS 174.61 / NRRL 6437.
  2. "Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae."
    Vainio A.E.I., Torkkeli H.T., Tuusa T., Aho S.A., Fagerstroem B.R., Korhola M.P.
    Curr. Genet. 24:38-44(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ATCC 20495 / CBS 174.61 / NRRL 6437.
  3. "Comparison of two glucoamylases from Hormoconis resinae."
    Fagerstroem R., Vainio A.E.I., Suoranta K., Pakula T., Kalkkinen N., Torkkeli H.T.
    J. Gen. Microbiol. 136:913-920(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 72-76, CHARACTERIZATION.

Entry informationi

Entry nameiAMYG_AMORE
AccessioniPrimary (citable) accession number: Q03045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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