Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q03045

- AMYG_AMORE

UniProt

Q03045 - AMYG_AMORE

Protein

Glucoamylase P

Gene

GAMP

Organism
Amorphotheca resinae (Creosote fungus) (Hormoconis resinae)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei149 – 1491SubstrateBy similarity
    Active sitei205 – 2051Proton acceptorBy similarity
    Active sitei208 – 2081Proton donorBy similarity

    GO - Molecular functioni

    1. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC
    2. starch binding Source: InterPro

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Protein family/group databases

    CAZyiCBM20. Carbohydrate-Binding Module Family 20.
    GH15. Glycoside Hydrolase Family 15.
    mycoCLAPiGLA15P_HORRE.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucoamylase P (EC:3.2.1.3)
    Alternative name(s):
    1,4-alpha-D-glucan glucohydrolase
    Glucan 1,4-alpha-glucosidase
    Gene namesi
    Name:GAMP
    OrganismiAmorphotheca resinae (Creosote fungus) (Hormoconis resinae)
    Taxonomic identifieri5101 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesLeotiomycetes incertae sedisMyxotrichaceaeAmorphotheca

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Add
    BLAST
    Chaini30 – 616587Glucoamylase PPRO_0000001471Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi427 – 4271N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ03045.
    SMRiQ03045. Positions 30-503.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini501 – 608108CBM20PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 15 family.Curated
    Contains 1 CBM20 (carbohydrate binding type-20) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    2.60.40.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR013784. Carb-bd-like_fold.
    IPR002044. CBM_fam20.
    IPR000165. Glucoamylase.
    IPR008291. Glucoamylase_SBD.
    IPR011613. Glyco_hydro_15.
    IPR013783. Ig-like_fold.
    [Graphical view]
    PfamiPF00686. CBM_20. 1 hit.
    PF00723. Glyco_hydro_15. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001031. Glu-a-glcsd_SBD. 1 hit.
    PRINTSiPR00736. GLHYDRLASE15.
    SMARTiSM01065. CBM_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF49452. SSF49452. 1 hit.
    PROSITEiPS51166. CBM20. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q03045-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLLPSSCAG ALSLLCSLAI AAPTELKARD LSSFIASERA IALQGALNNI    50
    GPDGSAVPGA GAGFVVASPS KANPDYFYTW SRDSALTLKM IIDEFILGNT 100
    TLQTIIEQYI HAQAVLQTVS NPSGTFLPDG VGLGEPKFMV DGTRFNGPWG 150
    RPQRDGPALR AIALMTYSNW LIKNGQFAEA KTKIWPIIAN DLSYVGQYWN 200
    QSGFDLWEET YASSFFTIQN QHRALVEGAQ LAHDLGVTCT GCDQAPEVLC 250
    FLQSFWNGKY IVSNINVNNG RTGLDGNSIL GAISTFDIDA YCDSPTLQPC 300
    HSQSLANFKV LTDTFRNLYT INAGIPEGQG VAVGRYAEDV YMGGNPWYLI 350
    TTAAAEFLYD AVAQWKARHV LTVDETSLAF FKDIYPEVTV REYKSGNANS 400
    PFAQIMDAVT AYADSYVAIA EKYIPSNGSL SEQFNRDTGT PLSAIDLTWS 450
    YAAFITMSQR RAGQYPSSWG SRNALPPPTT CSASSTPGIY TPATAAGAPN 500
    VTSSCQVSIT FNINATTYYG ENLYVIGNSS DLGAWNIADA YPLSASAYTQ 550
    DRPLWSAAIP LNAGEVISYQ YVRQEDCDQP YIYETVNRTL TVPACGGAAV 600
    TTDDAWMGPV GSSGNC 616
    Length:616
    Mass (Da):66,432
    Last modified:February 1, 1994 - v1
    Checksum:iB5F4DC8EEBB152FB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68143 Genomic DNA. Translation: CAA48243.1.
    X67708 mRNA. Translation: CAA47945.1.
    PIRiS33908.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68143 Genomic DNA. Translation: CAA48243.1 .
    X67708 mRNA. Translation: CAA47945.1 .
    PIRi S33908.

    3D structure databases

    ProteinModelPortali Q03045.
    SMRi Q03045. Positions 30-503.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM20. Carbohydrate-Binding Module Family 20.
    GH15. Glycoside Hydrolase Family 15.
    mycoCLAPi GLA15P_HORRE.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    2.60.40.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR013784. Carb-bd-like_fold.
    IPR002044. CBM_fam20.
    IPR000165. Glucoamylase.
    IPR008291. Glucoamylase_SBD.
    IPR011613. Glyco_hydro_15.
    IPR013783. Ig-like_fold.
    [Graphical view ]
    Pfami PF00686. CBM_20. 1 hit.
    PF00723. Glyco_hydro_15. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001031. Glu-a-glcsd_SBD. 1 hit.
    PRINTSi PR00736. GLHYDRLASE15.
    SMARTi SM01065. CBM_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF49452. SSF49452. 1 hit.
    PROSITEi PS51166. CBM20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Glucoamylase P gene of Hormoconis resinae: molecular cloning, sequencing and introduction into Trichoderma reesei."
      Joutsjoki V.V., Torkkeli T.K.
      FEMS Microbiol. Lett. 78:237-243(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 20495 / CBS 174.61 / NRRL 6437.
    2. "Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae."
      Vainio A.E.I., Torkkeli H.T., Tuusa T., Aho S.A., Fagerstroem B.R., Korhola M.P.
      Curr. Genet. 24:38-44(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ATCC 20495 / CBS 174.61 / NRRL 6437.
    3. "Comparison of two glucoamylases from Hormoconis resinae."
      Fagerstroem R., Vainio A.E.I., Suoranta K., Pakula T., Kalkkinen N., Torkkeli H.T.
      J. Gen. Microbiol. 136:913-920(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 72-76, CHARACTERIZATION.

    Entry informationi

    Entry nameiAMYG_AMORE
    AccessioniPrimary (citable) accession number: Q03045
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3