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Q03045

- AMYG_AMORE

UniProt

Q03045 - AMYG_AMORE

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Protein

Glucoamylase P

Gene

GAMP

Organism
Amorphotheca resinae (Creosote fungus) (Hormoconis resinae)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei149 – 1491SubstrateBy similarity
Active sitei205 – 2051Proton acceptorBy similarity
Active sitei208 – 2081Proton donorBy similarity

GO - Molecular functioni

  1. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC
  2. starch binding Source: InterPro

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH15. Glycoside Hydrolase Family 15.
mycoCLAPiGLA15P_HORRE.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase P (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:GAMP
OrganismiAmorphotheca resinae (Creosote fungus) (Hormoconis resinae)
Taxonomic identifieri5101 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesLeotiomycetes incertae sedisMyxotrichaceaeAmorphotheca

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Add
BLAST
Chaini30 – 616587Glucoamylase PPRO_0000001471Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi427 – 4271N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ03045.
SMRiQ03045. Positions 30-503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini501 – 608108CBM20PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated
Contains 1 CBM20 (carbohydrate binding type-20) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR000165. Glucoamylase.
IPR008291. Glucoamylase_SBD.
IPR011613. Glyco_hydro_15.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00686. CBM_20. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PIRSFiPIRSF001031. Glu-a-glcsd_SBD. 1 hit.
PRINTSiPR00736. GLHYDRLASE15.
SMARTiSM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49452. SSF49452. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03045-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLLPSSCAG ALSLLCSLAI AAPTELKARD LSSFIASERA IALQGALNNI
60 70 80 90 100
GPDGSAVPGA GAGFVVASPS KANPDYFYTW SRDSALTLKM IIDEFILGNT
110 120 130 140 150
TLQTIIEQYI HAQAVLQTVS NPSGTFLPDG VGLGEPKFMV DGTRFNGPWG
160 170 180 190 200
RPQRDGPALR AIALMTYSNW LIKNGQFAEA KTKIWPIIAN DLSYVGQYWN
210 220 230 240 250
QSGFDLWEET YASSFFTIQN QHRALVEGAQ LAHDLGVTCT GCDQAPEVLC
260 270 280 290 300
FLQSFWNGKY IVSNINVNNG RTGLDGNSIL GAISTFDIDA YCDSPTLQPC
310 320 330 340 350
HSQSLANFKV LTDTFRNLYT INAGIPEGQG VAVGRYAEDV YMGGNPWYLI
360 370 380 390 400
TTAAAEFLYD AVAQWKARHV LTVDETSLAF FKDIYPEVTV REYKSGNANS
410 420 430 440 450
PFAQIMDAVT AYADSYVAIA EKYIPSNGSL SEQFNRDTGT PLSAIDLTWS
460 470 480 490 500
YAAFITMSQR RAGQYPSSWG SRNALPPPTT CSASSTPGIY TPATAAGAPN
510 520 530 540 550
VTSSCQVSIT FNINATTYYG ENLYVIGNSS DLGAWNIADA YPLSASAYTQ
560 570 580 590 600
DRPLWSAAIP LNAGEVISYQ YVRQEDCDQP YIYETVNRTL TVPACGGAAV
610
TTDDAWMGPV GSSGNC
Length:616
Mass (Da):66,432
Last modified:February 1, 1994 - v1
Checksum:iB5F4DC8EEBB152FB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68143 Genomic DNA. Translation: CAA48243.1.
X67708 mRNA. Translation: CAA47945.1.
PIRiS33908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68143 Genomic DNA. Translation: CAA48243.1 .
X67708 mRNA. Translation: CAA47945.1 .
PIRi S33908.

3D structure databases

ProteinModelPortali Q03045.
SMRi Q03045. Positions 30-503.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM20. Carbohydrate-Binding Module Family 20.
GH15. Glycoside Hydrolase Family 15.
mycoCLAPi GLA15P_HORRE.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR000165. Glucoamylase.
IPR008291. Glucoamylase_SBD.
IPR011613. Glyco_hydro_15.
IPR013783. Ig-like_fold.
[Graphical view ]
Pfami PF00686. CBM_20. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view ]
PIRSFi PIRSF001031. Glu-a-glcsd_SBD. 1 hit.
PRINTSi PR00736. GLHYDRLASE15.
SMARTi SM01065. CBM_2. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
SSF49452. SSF49452. 1 hit.
PROSITEi PS51166. CBM20. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Glucoamylase P gene of Hormoconis resinae: molecular cloning, sequencing and introduction into Trichoderma reesei."
    Joutsjoki V.V., Torkkeli T.K.
    FEMS Microbiol. Lett. 78:237-243(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 20495 / CBS 174.61 / NRRL 6437.
  2. "Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae."
    Vainio A.E.I., Torkkeli H.T., Tuusa T., Aho S.A., Fagerstroem B.R., Korhola M.P.
    Curr. Genet. 24:38-44(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ATCC 20495 / CBS 174.61 / NRRL 6437.
  3. "Comparison of two glucoamylases from Hormoconis resinae."
    Fagerstroem R., Vainio A.E.I., Suoranta K., Pakula T., Kalkkinen N., Torkkeli H.T.
    J. Gen. Microbiol. 136:913-920(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 72-76, CHARACTERIZATION.

Entry informationi

Entry nameiAMYG_AMORE
AccessioniPrimary (citable) accession number: Q03045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3