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Protein

Glucoamylase P

Gene

GAMP

Organism
Amorphotheca resinae (Creosote fungus) (Hormoconis resinae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149SubstrateBy similarity1
Active sitei205Proton acceptorBy similarity1
Active sitei208Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH15. Glycoside Hydrolase Family 15.
mycoCLAPiGLA15P_AMORE.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase P (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:GAMP
OrganismiAmorphotheca resinae (Creosote fungus) (Hormoconis resinae)
Taxonomic identifieri5101 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesLeotiomycetes incertae sedisMyxotrichaceaeAmorphotheca

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Add BLAST29
ChainiPRO_000000147130 – 616Glucoamylase PAdd BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi200N-linked (GlcNAc...)Sequence analysis1
Glycosylationi427N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ03045.

Structurei

3D structure databases

ProteinModelPortaliQ03045.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini501 – 608CBM20PROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated
Contains 1 CBM20 (carbohydrate binding type-20) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR000165. Glucoamylase.
IPR008291. Glucoamylase_SBD.
IPR011613. Glyco_hydro_15/PHK.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00686. CBM_20. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PIRSFiPIRSF001031. Glu-a-glcsd_SBD. 1 hit.
PRINTSiPR00736. GLHYDRLASE15.
SMARTiSM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49452. SSF49452. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLPSSCAG ALSLLCSLAI AAPTELKARD LSSFIASERA IALQGALNNI
60 70 80 90 100
GPDGSAVPGA GAGFVVASPS KANPDYFYTW SRDSALTLKM IIDEFILGNT
110 120 130 140 150
TLQTIIEQYI HAQAVLQTVS NPSGTFLPDG VGLGEPKFMV DGTRFNGPWG
160 170 180 190 200
RPQRDGPALR AIALMTYSNW LIKNGQFAEA KTKIWPIIAN DLSYVGQYWN
210 220 230 240 250
QSGFDLWEET YASSFFTIQN QHRALVEGAQ LAHDLGVTCT GCDQAPEVLC
260 270 280 290 300
FLQSFWNGKY IVSNINVNNG RTGLDGNSIL GAISTFDIDA YCDSPTLQPC
310 320 330 340 350
HSQSLANFKV LTDTFRNLYT INAGIPEGQG VAVGRYAEDV YMGGNPWYLI
360 370 380 390 400
TTAAAEFLYD AVAQWKARHV LTVDETSLAF FKDIYPEVTV REYKSGNANS
410 420 430 440 450
PFAQIMDAVT AYADSYVAIA EKYIPSNGSL SEQFNRDTGT PLSAIDLTWS
460 470 480 490 500
YAAFITMSQR RAGQYPSSWG SRNALPPPTT CSASSTPGIY TPATAAGAPN
510 520 530 540 550
VTSSCQVSIT FNINATTYYG ENLYVIGNSS DLGAWNIADA YPLSASAYTQ
560 570 580 590 600
DRPLWSAAIP LNAGEVISYQ YVRQEDCDQP YIYETVNRTL TVPACGGAAV
610
TTDDAWMGPV GSSGNC
Length:616
Mass (Da):66,432
Last modified:February 1, 1994 - v1
Checksum:iB5F4DC8EEBB152FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68143 Genomic DNA. Translation: CAA48243.1.
X67708 mRNA. Translation: CAA47945.1.
PIRiS33908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68143 Genomic DNA. Translation: CAA48243.1.
X67708 mRNA. Translation: CAA47945.1.
PIRiS33908.

3D structure databases

ProteinModelPortaliQ03045.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH15. Glycoside Hydrolase Family 15.
mycoCLAPiGLA15P_AMORE.

Proteomic databases

PRIDEiQ03045.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR000165. Glucoamylase.
IPR008291. Glucoamylase_SBD.
IPR011613. Glyco_hydro_15/PHK.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00686. CBM_20. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PIRSFiPIRSF001031. Glu-a-glcsd_SBD. 1 hit.
PRINTSiPR00736. GLHYDRLASE15.
SMARTiSM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49452. SSF49452. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMYG_AMORE
AccessioniPrimary (citable) accession number: Q03045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 5, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.