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Q03043

- KGP24_DROME

UniProt

Q03043 - KGP24_DROME

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Protein

cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B

Gene

for

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei807 – 8071ATPPROSITE-ProRule annotation
Active sitei901 – 9011Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi529 – 645117cGMP 1By similarityAdd
BLAST
Nucleotide bindingi646 – 761116cGMP 2By similarityAdd
BLAST
Nucleotide bindingi783 – 7919ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cGMP binding Source: UniProtKB-KW
  3. cGMP-dependent protein kinase activity Source: FlyBase
  4. cyclic nucleotide-dependent protein kinase activity Source: FlyBase
  5. protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  1. feeding behavior Source: FlyBase
  2. habituation Source: FlyBase
  3. larval feeding behavior Source: UniProtKB
  4. larval locomotory behavior Source: FlyBase
  5. long-term memory Source: FlyBase
  6. protein phosphorylation Source: UniProtKB
  7. regulation of heart contraction Source: FlyBase
  8. regulation of response to food Source: FlyBase
  9. response to sucrose Source: FlyBase
  10. short-term memory Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, cGMP, cGMP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.12. 1994.
ReactomeiREACT_184321. Ca2+ pathway.
SignaLinkiQ03043.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B (EC:2.7.11.12)
Short name:
cGK
Alternative name(s):
Foraging protein
Gene namesi
Name:for
Synonyms:DG2, PGK2, Pkg24A
ORF Names:CG10033
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000721. for.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10881088cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3BPRO_0000086121Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei934 – 9341Phosphothreonine1 Publication
Modified residuei938 – 9381Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ03043.

Expressioni

Developmental stagei

Expressed throughout development, high during embryonic and adult stages. Isoform T1 is predominantly expressed during embryo and adult stages.1 Publication

Gene expression databases

BgeeiQ03043.
ExpressionAtlasiQ03043. differential.

Interactioni

Protein-protein interaction databases

BioGridi69303. 9 interactions.
MINTiMINT-795797.

Structurei

3D structure databases

ProteinModelPortaliQ03043.
SMRiQ03043. Positions 475-1072.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini777 – 1036260Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini1037 – 108852AGC-kinase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6969DimerizationBy similarityAdd
BLAST
Regioni70 – 528459RegulatoryBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074358.
InParanoidiQ03043.
KOiK07376.
OMAiQMQGLRI.
OrthoDBiEOG75B84Q.
PhylomeDBiQ03043.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR000961. AGC-kinase_C.
IPR002374. cGMP_dep_kinase.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00104. CGMPKINASE.
SMARTiSM00100. cNMP. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform T1 (identifier: Q03043-1) [UniParc]FASTAAdd to Basket

Also known as: A, H

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRFCFDRLCF ATKRPAQNSN SNAPHSSTTV DAPPRPADVD VATVPVATPA
60 70 80 90 100
PPPQQPVSNL FYADYQKLQP AIIDRDWERD RDTDTDTRSE AKPPDIVEHI
110 120 130 140 150
EPVEEQRQIH TQIQSPAEIQ IQIPPTPPAP SIQIQIQQRY RRHSSAEDRN
160 170 180 190 200
LNTRRNDSNI TEALRKAASM QQEPNANYQF PTDLGLVSIV NNNNNTNTHP
210 220 230 240 250
SGSNSGSNNN SNINNNLVGG IVTLPAAGGL IGLEHTASGL RLIPAPPTHS
260 270 280 290 300
DVLTHTLIYG TPPSGAQQLN QDPRSLLHQQ ELQLQQRYQQ LQQLQAQTQG
310 320 330 340 350
LYTSQGSPVL YHQPSPGSSQ PVAIPGATCH SPTQLQPPNT LNLQQQMQSL
360 370 380 390 400
RISGCTPSGT GGSATPSPVG LVDPNFIVSN YVAASPQEER FIQIIQAKEL
410 420 430 440 450
KIQEMQRALQ FKDNEIAELK SHLDKFQSVF PFSRGSAAGC AGTGGASGSG
460 470 480 490 500
AGGSGGSGPG TATGATRKSG QNFQRQRALG ISAEPQSESS LLLEHVSFPK
510 520 530 540 550
YDKDERSREL IKAAILDNDF MKNLDLTQIR EIVDCMYPVK YPAKNLIIKE
560 570 580 590 600
GDVGSIVYVM EDGRVEVSRE GKYLSTLSGA KVLGELAILY NCQRTATITA
610 620 630 640 650
ITECNLWAIE RQCFQTIMMR TGLIRQAEYS DFLKSVPIFK DLAEDTLIKI
660 670 680 690 700
SDVLEETHYQ RGDYIVRQGA RGDTFFIISK GKVRVTIKQQ DTQEEKFIRM
710 720 730 740 750
LGKGDFFGEK ALQGDDLRTA NIICESADGV SCLVIDRETF NQLISNLDEI
760 770 780 790 800
KHRYDDEGAM ERRKINEEFR DINLTDLRVI ATLGVGGFGR VELVQTNGDS
810 820 830 840 850
SRSFALKQMK KSQIVETRQQ QHIMSEKEIM GEANCQFIVK LFKTFKDKKY
860 870 880 890 900
LYMLMESCLG GELWTILRDK GNFDDSTTRF YTACVVEAFD YLHSRNIIYR
910 920 930 940 950
DLKPENLLLN ERGYVKLVDF GFAKKLQTGR KTWTFCGTPE YVAPEVILNR
960 970 980 990 1000
GHDISADYWS LGVLMFELLT GTPPFTGSDP MRTYNIILKG IDAIEFPRNI
1010 1020 1030 1040 1050
TRNASNLIKK LCRDNPAERL GYQRGGISEI QKHKWFDGFY WWGLQNCTLE
1060 1070 1080
PPIKPAVKSV VDTTNFDDYP PDPEGPPPDD VTGWDKDF
Length:1,088
Mass (Da):121,341
Last modified:February 1, 2005 - v3
Checksum:iA9450E31FF7AB8BD
GO
Isoform cD4 (identifier: Q03043-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-520: Missing.

Show »
Length:568
Mass (Da):65,073
Checksum:iA5A275D0B5A50397
GO
Isoform T3A (identifier: Q03043-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.

Show »
Length:919
Mass (Da):102,235
Checksum:iFD7DDDFC3391F3AD
GO
Isoform T3B (identifier: Q03043-3) [UniParc]FASTAAdd to Basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-346: Missing.

Show »
Length:742
Mass (Da):83,349
Checksum:i8354AAA1630DCE09
GO
Isoform cD5 (identifier: P32023-1) [UniParc]FASTAAdd to Basket

Also known as: E, J

The sequence of this isoform can be found in the external entry P32023.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:934
Mass (Da):105,907
GO
Isoform T2 (identifier: P32023-2) [UniParc]FASTAAdd to Basket

Also known as: C, D, F, G, T2a, T2b

The sequence of this isoform can be found in the external entry P32023.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:894
Mass (Da):101,058
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491P → T in AAA28455. (PubMed:2732245)Curated
Sequence conflicti207 – 2071S → T in AAA28455. (PubMed:2732245)Curated
Sequence conflicti664 – 6641Y → H in AAA28455. (PubMed:2732245)Curated
Sequence conflicti664 – 6641Y → H in AAA28459. (PubMed:2732245)Curated
Sequence conflicti692 – 6921T → R in AAA28455. (PubMed:2732245)Curated
Sequence conflicti692 – 6921T → R in AAA28459. (PubMed:2732245)Curated
Sequence conflicti915 – 9151V → G in AAA28455. (PubMed:2732245)Curated
Sequence conflicti915 – 9151V → G in AAA28459. (PubMed:2732245)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 520520Missing in isoform cD4. CuratedVSP_004763Add
BLAST
Alternative sequencei1 – 346346Missing in isoform T3B. 1 PublicationVSP_004762Add
BLAST
Alternative sequencei1 – 169169Missing in isoform T3A. CuratedVSP_004761Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27120
, M27117, M27118, M27119 Genomic DNA. Translation: AAA28455.1.
M30413 mRNA. Translation: AAA28459.1.
AE014134 Genomic DNA. Translation: AAF51082.2.
AE014134 Genomic DNA. Translation: AAG22251.1.
AE014134 Genomic DNA. Translation: AAS64613.1.
AE014134 Genomic DNA. Translation: AAS64614.1.
AY061514 mRNA. Translation: AAL29062.1.
PIRiB34106.
RefSeqiNP_477487.1. NM_058139.4. [Q03043-1]
NP_995626.1. NM_205904.2. [Q03043-1]
NP_995628.1. NM_205906.2. [Q03043-1]
UniGeneiDm.6584.

Genome annotation databases

EnsemblMetazoaiFBtr0089304; FBpp0088350; FBgn0000721. [Q03043-1]
FBtr0089310; FBpp0088922; FBgn0000721. [Q03043-1]
FBtr0089312; FBpp0088923; FBgn0000721. [Q03043-1]
GeneIDi44817.
KEGGidme:Dmel_CG10033.
UCSCiCG10033-RA. d. melanogaster. [Q03043-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27120
, M27117 , M27118 , M27119 Genomic DNA. Translation: AAA28455.1 .
M30413 mRNA. Translation: AAA28459.1 .
AE014134 Genomic DNA. Translation: AAF51082.2 .
AE014134 Genomic DNA. Translation: AAG22251.1 .
AE014134 Genomic DNA. Translation: AAS64613.1 .
AE014134 Genomic DNA. Translation: AAS64614.1 .
AY061514 mRNA. Translation: AAL29062.1 .
PIRi B34106.
RefSeqi NP_477487.1. NM_058139.4. [Q03043-1 ]
NP_995626.1. NM_205904.2. [Q03043-1 ]
NP_995628.1. NM_205906.2. [Q03043-1 ]
UniGenei Dm.6584.

3D structure databases

ProteinModelPortali Q03043.
SMRi Q03043. Positions 475-1072.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 69303. 9 interactions.
MINTi MINT-795797.

Proteomic databases

PaxDbi Q03043.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0089304 ; FBpp0088350 ; FBgn0000721 . [Q03043-1 ]
FBtr0089310 ; FBpp0088922 ; FBgn0000721 . [Q03043-1 ]
FBtr0089312 ; FBpp0088923 ; FBgn0000721 . [Q03043-1 ]
GeneIDi 44817.
KEGGi dme:Dmel_CG10033.
UCSCi CG10033-RA. d. melanogaster. [Q03043-1 ]

Organism-specific databases

CTDi 44817.
FlyBasei FBgn0000721. for.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074358.
InParanoidi Q03043.
KOi K07376.
OMAi QMQGLRI.
OrthoDBi EOG75B84Q.
PhylomeDBi Q03043.

Enzyme and pathway databases

BRENDAi 2.7.11.12. 1994.
Reactomei REACT_184321. Ca2+ pathway.
SignaLinki Q03043.

Miscellaneous databases

GenomeRNAii 44817.
NextBioi 837662.
PROi Q03043.

Gene expression databases

Bgeei Q03043.
ExpressionAtlasi Q03043. differential.

Family and domain databases

Gene3Di 2.60.120.10. 2 hits.
InterProi IPR000961. AGC-kinase_C.
IPR002374. cGMP_dep_kinase.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR00104. CGMPKINASE.
SMARTi SM00100. cNMP. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cGMP-dependent protein kinase genes in Drosophila."
    Kalderon D., Rubin G.M.
    J. Biol. Chem. 264:10738-10748(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS T1 AND T3B), DEVELOPMENTAL STAGE.
    Strain: Canton-S.
    Tissue: Imaginal disk.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM T1).
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-934 AND THR-938, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiKGP24_DROME
AccessioniPrimary (citable) accession number: Q03043
Secondary accession number(s): A4V042
, Q24304, Q9I7Q1, Q9VQT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 2005
Last modified: October 29, 2014
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3