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Protein

cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B

Gene

for

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei699cGMP 2By similarity1
Binding sitei754cAMP or cGMP 2By similarity1
Binding sitei807ATPPROSITE-ProRule annotation1
Active sitei901Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi584 – 587cAMP or cGMP 1By similarity4
Nucleotide bindingi594 – 595cAMP or cGMP 1By similarity2
Nucleotide bindingi708 – 711cAMP or cGMP 2By similarity4
Nucleotide bindingi718 – 719cAMP or cGMP 2By similarity2
Nucleotide bindingi783 – 791ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cGMP binding Source: UniProtKB-KW
  • cGMP-dependent protein kinase activity Source: FlyBase
  • cyclic nucleotide-dependent protein kinase activity Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  • feeding behavior Source: FlyBase
  • habituation Source: FlyBase
  • larval feeding behavior Source: UniProtKB
  • larval locomotory behavior Source: FlyBase
  • long-term memory Source: FlyBase
  • motor neuron axon guidance Source: FlyBase
  • protein phosphorylation Source: UniProtKB
  • regulation of BMP signaling pathway Source: FlyBase
  • regulation of heart contraction Source: FlyBase
  • regulation of response to food Source: FlyBase
  • response to sucrose Source: FlyBase
  • short-term memory Source: FlyBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, cGMP, cGMP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.12 1994
ReactomeiR-DME-392517 Rap1 signalling
SignaLinkiQ03043

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B (EC:2.7.11.12)
Short name:
cGK
Alternative name(s):
Foraging protein
Gene namesi
Name:for
Synonyms:DG2, PGK2, Pkg24A
ORF Names:CG10033
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000721 for

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861211 – 1088cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3BAdd BLAST1088

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei934Phosphothreonine1 Publication1
Modified residuei938Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ03043
PRIDEiQ03043

PTM databases

iPTMnetiQ03043

Expressioni

Developmental stagei

Expressed throughout development, high during embryonic and adult stages. Isoform T1 is predominantly expressed during embryo and adult stages.1 Publication

Gene expression databases

BgeeiFBgn0000721
ExpressionAtlasiQ03043 differential
GenevisibleiQ03043 DM

Interactioni

Protein-protein interaction databases

BioGridi6930312 interactors.
IntActiQ03043 9 interactors.
STRINGi7227.FBpp0088350

Structurei

3D structure databases

ProteinModelPortaliQ03043
SMRiQ03043
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini777 – 1036Protein kinasePROSITE-ProRule annotationAdd BLAST260
Domaini1037 – 1088AGC-kinase C-terminalAdd BLAST52

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 69DimerizationBy similarityAdd BLAST69
Regioni70 – 528RegulatoryBy similarityAdd BLAST459

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0616 Eukaryota
ENOG410XPQQ LUCA
GeneTreeiENSGT00810000125385
InParanoidiQ03043
OrthoDBiEOG091G0S9R
PhylomeDBiQ03043

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
cd05572 STKc_cGK, 1 hit
Gene3Di2.60.120.102 hits
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR002374 cGMP_dep_kinase
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR011009 Kinase-like_dom_sf
IPR031831 PKcGMP_CC
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR014710 RmlC-like_jellyroll
IPR008271 Ser/Thr_kinase_AS
IPR035014 STKc_cGK
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF16808 PKcGMP_CC, 1 hit
PF00069 Pkinase, 1 hit
PRINTSiPR00104 CGMPKINASE
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform T1 (identifier: Q03043-1) [UniParc]FASTAAdd to basket
Also known as: A, H

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRFCFDRLCF ATKRPAQNSN SNAPHSSTTV DAPPRPADVD VATVPVATPA
60 70 80 90 100
PPPQQPVSNL FYADYQKLQP AIIDRDWERD RDTDTDTRSE AKPPDIVEHI
110 120 130 140 150
EPVEEQRQIH TQIQSPAEIQ IQIPPTPPAP SIQIQIQQRY RRHSSAEDRN
160 170 180 190 200
LNTRRNDSNI TEALRKAASM QQEPNANYQF PTDLGLVSIV NNNNNTNTHP
210 220 230 240 250
SGSNSGSNNN SNINNNLVGG IVTLPAAGGL IGLEHTASGL RLIPAPPTHS
260 270 280 290 300
DVLTHTLIYG TPPSGAQQLN QDPRSLLHQQ ELQLQQRYQQ LQQLQAQTQG
310 320 330 340 350
LYTSQGSPVL YHQPSPGSSQ PVAIPGATCH SPTQLQPPNT LNLQQQMQSL
360 370 380 390 400
RISGCTPSGT GGSATPSPVG LVDPNFIVSN YVAASPQEER FIQIIQAKEL
410 420 430 440 450
KIQEMQRALQ FKDNEIAELK SHLDKFQSVF PFSRGSAAGC AGTGGASGSG
460 470 480 490 500
AGGSGGSGPG TATGATRKSG QNFQRQRALG ISAEPQSESS LLLEHVSFPK
510 520 530 540 550
YDKDERSREL IKAAILDNDF MKNLDLTQIR EIVDCMYPVK YPAKNLIIKE
560 570 580 590 600
GDVGSIVYVM EDGRVEVSRE GKYLSTLSGA KVLGELAILY NCQRTATITA
610 620 630 640 650
ITECNLWAIE RQCFQTIMMR TGLIRQAEYS DFLKSVPIFK DLAEDTLIKI
660 670 680 690 700
SDVLEETHYQ RGDYIVRQGA RGDTFFIISK GKVRVTIKQQ DTQEEKFIRM
710 720 730 740 750
LGKGDFFGEK ALQGDDLRTA NIICESADGV SCLVIDRETF NQLISNLDEI
760 770 780 790 800
KHRYDDEGAM ERRKINEEFR DINLTDLRVI ATLGVGGFGR VELVQTNGDS
810 820 830 840 850
SRSFALKQMK KSQIVETRQQ QHIMSEKEIM GEANCQFIVK LFKTFKDKKY
860 870 880 890 900
LYMLMESCLG GELWTILRDK GNFDDSTTRF YTACVVEAFD YLHSRNIIYR
910 920 930 940 950
DLKPENLLLN ERGYVKLVDF GFAKKLQTGR KTWTFCGTPE YVAPEVILNR
960 970 980 990 1000
GHDISADYWS LGVLMFELLT GTPPFTGSDP MRTYNIILKG IDAIEFPRNI
1010 1020 1030 1040 1050
TRNASNLIKK LCRDNPAERL GYQRGGISEI QKHKWFDGFY WWGLQNCTLE
1060 1070 1080
PPIKPAVKSV VDTTNFDDYP PDPEGPPPDD VTGWDKDF
Length:1,088
Mass (Da):121,341
Last modified:February 1, 2005 - v3
Checksum:iA9450E31FF7AB8BD
GO
Isoform cD4 (identifier: Q03043-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-520: Missing.

Show »
Length:568
Mass (Da):65,073
Checksum:iA5A275D0B5A50397
GO
Isoform T3A (identifier: Q03043-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.

Show »
Length:919
Mass (Da):102,235
Checksum:iFD7DDDFC3391F3AD
GO
Isoform T3B (identifier: Q03043-3) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-346: Missing.

Show »
Length:742
Mass (Da):83,349
Checksum:i8354AAA1630DCE09
GO
Isoform cD5 (identifier: P32023-1) [UniParc]FASTAAdd to basket
Also known as: E, J
The sequence of this isoform can be found in the external entry P32023.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:934
Mass (Da):105,907
GO
Isoform T2 (identifier: P32023-2) [UniParc]FASTAAdd to basket
Also known as: C, D, G, T2a, T2b
The sequence of this isoform can be found in the external entry P32023.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:894
Mass (Da):101,058
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49P → T in AAA28455 (PubMed:2732245).Curated1
Sequence conflicti207S → T in AAA28455 (PubMed:2732245).Curated1
Sequence conflicti664Y → H in AAA28455 (PubMed:2732245).Curated1
Sequence conflicti664Y → H in AAA28459 (PubMed:2732245).Curated1
Sequence conflicti692T → R in AAA28455 (PubMed:2732245).Curated1
Sequence conflicti692T → R in AAA28459 (PubMed:2732245).Curated1
Sequence conflicti915V → G in AAA28455 (PubMed:2732245).Curated1
Sequence conflicti915V → G in AAA28459 (PubMed:2732245).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0047631 – 520Missing in isoform cD4. CuratedAdd BLAST520
Alternative sequenceiVSP_0047621 – 346Missing in isoform T3B. 1 PublicationAdd BLAST346
Alternative sequenceiVSP_0047611 – 169Missing in isoform T3A. CuratedAdd BLAST169

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27120
, M27117, M27118, M27119 Genomic DNA Translation: AAA28455.1
M30413 mRNA Translation: AAA28459.1
AE014134 Genomic DNA Translation: AAF51082.2
AE014134 Genomic DNA Translation: AAS64613.1
AE014134 Genomic DNA Translation: AAS64614.1
AY061514 mRNA Translation: AAL29062.1
PIRiB34106
RefSeqiNP_477487.1, NM_058139.4 [Q03043-1]
NP_995626.1, NM_205904.2 [Q03043-1]
NP_995628.1, NM_205906.2 [Q03043-1]
UniGeneiDm.6584

Genome annotation databases

EnsemblMetazoaiFBtr0089304; FBpp0088350; FBgn0000721 [Q03043-1]
FBtr0089310; FBpp0088922; FBgn0000721 [Q03043-1]
FBtr0089312; FBpp0088923; FBgn0000721 [Q03043-1]
FBtr0446099; FBpp0402801; FBgn0000721 [Q03043-3]
GeneIDi44817
UCSCiCG10033-RA d. melanogaster [Q03043-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKGP24_DROME
AccessioniPrimary (citable) accession number: Q03043
Secondary accession number(s): A4V042
, Q24304, Q9I7Q1, Q9VQT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 2005
Last modified: March 28, 2018
This is version 173 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome