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Q03043

- KGP24_DROME

UniProt

Q03043 - KGP24_DROME

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Protein

cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B

Gene
for, DG2, PGK2, Pkg24A, CG10033
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei807 – 8071ATP By similarity
Active sitei901 – 9011Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi529 – 645117cGMP 1 By similarityAdd
BLAST
Nucleotide bindingi646 – 761116cGMP 2 By similarityAdd
BLAST
Nucleotide bindingi783 – 7919ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cGMP binding Source: UniProtKB-KW
  3. cGMP-dependent protein kinase activity Source: UniProtKB
  4. cyclic nucleotide-dependent protein kinase activity Source: FlyBase
  5. protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  1. feeding behavior Source: FlyBase
  2. habituation Source: FlyBase
  3. larval feeding behavior Source: UniProtKB
  4. larval locomotory behavior Source: FlyBase
  5. long-term memory Source: FlyBase
  6. protein phosphorylation Source: UniProtKB
  7. regulation of heart contraction Source: FlyBase
  8. regulation of response to food Source: FlyBase
  9. response to sucrose Source: FlyBase
  10. short-term memory Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, cGMP, cGMP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.12. 1994.
ReactomeiREACT_184321. Ca2+ pathway.
SignaLinkiQ03043.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B (EC:2.7.11.12)
Short name:
cGK
Alternative name(s):
Foraging protein
Gene namesi
Name:for
Synonyms:DG2, PGK2, Pkg24A
ORF Names:CG10033
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000721. for.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10881088cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3BPRO_0000086121Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei934 – 9341Phosphothreonine1 Publication
Modified residuei938 – 9381Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ03043.

Expressioni

Developmental stagei

Expressed throughout development, high during embryonic and adult stages. Isoform T1 is predominantly expressed during embryo and adult stages.1 Publication

Gene expression databases

BgeeiQ03043.

Interactioni

Protein-protein interaction databases

BioGridi69303. 9 interactions.
MINTiMINT-795797.

Structurei

3D structure databases

ProteinModelPortaliQ03043.
SMRiQ03043. Positions 389-430, 475-1072.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini777 – 1036260Protein kinaseAdd
BLAST
Domaini1037 – 108852AGC-kinase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6969Dimerization By similarityAdd
BLAST
Regioni70 – 528459Regulatory By similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074358.
InParanoidiQ03043.
KOiK07376.
OMAiQMQGLRI.
OrthoDBiEOG75B84Q.
PhylomeDBiQ03043.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR000961. AGC-kinase_C.
IPR002374. cGMP_dep_kinase.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00104. CGMPKINASE.
SMARTiSM00100. cNMP. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform T1 (identifier: Q03043-1) [UniParc]FASTAAdd to Basket

Also known as: A, H

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRFCFDRLCF ATKRPAQNSN SNAPHSSTTV DAPPRPADVD VATVPVATPA     50
PPPQQPVSNL FYADYQKLQP AIIDRDWERD RDTDTDTRSE AKPPDIVEHI 100
EPVEEQRQIH TQIQSPAEIQ IQIPPTPPAP SIQIQIQQRY RRHSSAEDRN 150
LNTRRNDSNI TEALRKAASM QQEPNANYQF PTDLGLVSIV NNNNNTNTHP 200
SGSNSGSNNN SNINNNLVGG IVTLPAAGGL IGLEHTASGL RLIPAPPTHS 250
DVLTHTLIYG TPPSGAQQLN QDPRSLLHQQ ELQLQQRYQQ LQQLQAQTQG 300
LYTSQGSPVL YHQPSPGSSQ PVAIPGATCH SPTQLQPPNT LNLQQQMQSL 350
RISGCTPSGT GGSATPSPVG LVDPNFIVSN YVAASPQEER FIQIIQAKEL 400
KIQEMQRALQ FKDNEIAELK SHLDKFQSVF PFSRGSAAGC AGTGGASGSG 450
AGGSGGSGPG TATGATRKSG QNFQRQRALG ISAEPQSESS LLLEHVSFPK 500
YDKDERSREL IKAAILDNDF MKNLDLTQIR EIVDCMYPVK YPAKNLIIKE 550
GDVGSIVYVM EDGRVEVSRE GKYLSTLSGA KVLGELAILY NCQRTATITA 600
ITECNLWAIE RQCFQTIMMR TGLIRQAEYS DFLKSVPIFK DLAEDTLIKI 650
SDVLEETHYQ RGDYIVRQGA RGDTFFIISK GKVRVTIKQQ DTQEEKFIRM 700
LGKGDFFGEK ALQGDDLRTA NIICESADGV SCLVIDRETF NQLISNLDEI 750
KHRYDDEGAM ERRKINEEFR DINLTDLRVI ATLGVGGFGR VELVQTNGDS 800
SRSFALKQMK KSQIVETRQQ QHIMSEKEIM GEANCQFIVK LFKTFKDKKY 850
LYMLMESCLG GELWTILRDK GNFDDSTTRF YTACVVEAFD YLHSRNIIYR 900
DLKPENLLLN ERGYVKLVDF GFAKKLQTGR KTWTFCGTPE YVAPEVILNR 950
GHDISADYWS LGVLMFELLT GTPPFTGSDP MRTYNIILKG IDAIEFPRNI 1000
TRNASNLIKK LCRDNPAERL GYQRGGISEI QKHKWFDGFY WWGLQNCTLE 1050
PPIKPAVKSV VDTTNFDDYP PDPEGPPPDD VTGWDKDF 1088
Length:1,088
Mass (Da):121,341
Last modified:February 1, 2005 - v3
Checksum:iA9450E31FF7AB8BD
GO
Isoform cD4 (identifier: Q03043-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-520: Missing.

Show »
Length:568
Mass (Da):65,073
Checksum:iA5A275D0B5A50397
GO
Isoform T3A (identifier: Q03043-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.

Show »
Length:919
Mass (Da):102,235
Checksum:iFD7DDDFC3391F3AD
GO
Isoform T3B (identifier: Q03043-3) [UniParc]FASTAAdd to Basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-346: Missing.

Show »
Length:742
Mass (Da):83,349
Checksum:i8354AAA1630DCE09
GO
Isoform cD5 (identifier: P32023-1) [UniParc]FASTAAdd to Basket

Also known as: E, J

The sequence of this isoform can be found in the external entry P32023.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:934
Mass (Da):105,907
GO
Isoform T2 (identifier: P32023-2) [UniParc]FASTAAdd to Basket

Also known as: C, D, F, G, T2a, T2b

The sequence of this isoform can be found in the external entry P32023.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:894
Mass (Da):101,058
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 520520Missing in isoform cD4. VSP_004763Add
BLAST
Alternative sequencei1 – 346346Missing in isoform T3B. VSP_004762Add
BLAST
Alternative sequencei1 – 169169Missing in isoform T3A. VSP_004761Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491P → T in AAA28455. 1 Publication
Sequence conflicti207 – 2071S → T in AAA28455. 1 Publication
Sequence conflicti664 – 6641Y → H in AAA28455. 1 Publication
Sequence conflicti664 – 6641Y → H in AAA28459. 1 Publication
Sequence conflicti692 – 6921T → R in AAA28455. 1 Publication
Sequence conflicti692 – 6921T → R in AAA28459. 1 Publication
Sequence conflicti915 – 9151V → G in AAA28455. 1 Publication
Sequence conflicti915 – 9151V → G in AAA28459. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27120
, M27117, M27118, M27119 Genomic DNA. Translation: AAA28455.1.
M30413 mRNA. Translation: AAA28459.1.
AE014134 Genomic DNA. Translation: AAF51082.2.
AE014134 Genomic DNA. Translation: AAG22251.1.
AE014134 Genomic DNA. Translation: AAS64613.1.
AE014134 Genomic DNA. Translation: AAS64614.1.
AY061514 mRNA. Translation: AAL29062.1.
PIRiB34106.
RefSeqiNP_477487.1. NM_058139.3. [Q03043-1]
NP_477488.1. NM_058140.3. [Q03043-3]
NP_995626.1. NM_205904.1. [Q03043-1]
NP_995628.1. NM_205906.1. [Q03043-1]
UniGeneiDm.6584.

Genome annotation databases

EnsemblMetazoaiFBtr0089304; FBpp0088350; FBgn0000721. [Q03043-1]
FBtr0089310; FBpp0088922; FBgn0000721. [Q03043-1]
FBtr0089312; FBpp0088923; FBgn0000721. [Q03043-1]
GeneIDi44817.
KEGGidme:Dmel_CG10033.
UCSCiCG10033-RA. d. melanogaster. [Q03043-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27120
, M27117 , M27118 , M27119 Genomic DNA. Translation: AAA28455.1 .
M30413 mRNA. Translation: AAA28459.1 .
AE014134 Genomic DNA. Translation: AAF51082.2 .
AE014134 Genomic DNA. Translation: AAG22251.1 .
AE014134 Genomic DNA. Translation: AAS64613.1 .
AE014134 Genomic DNA. Translation: AAS64614.1 .
AY061514 mRNA. Translation: AAL29062.1 .
PIRi B34106.
RefSeqi NP_477487.1. NM_058139.3. [Q03043-1 ]
NP_477488.1. NM_058140.3. [Q03043-3 ]
NP_995626.1. NM_205904.1. [Q03043-1 ]
NP_995628.1. NM_205906.1. [Q03043-1 ]
UniGenei Dm.6584.

3D structure databases

ProteinModelPortali Q03043.
SMRi Q03043. Positions 389-430, 475-1072.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 69303. 9 interactions.
MINTi MINT-795797.

Proteomic databases

PaxDbi Q03043.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0089304 ; FBpp0088350 ; FBgn0000721 . [Q03043-1 ]
FBtr0089310 ; FBpp0088922 ; FBgn0000721 . [Q03043-1 ]
FBtr0089312 ; FBpp0088923 ; FBgn0000721 . [Q03043-1 ]
GeneIDi 44817.
KEGGi dme:Dmel_CG10033.
UCSCi CG10033-RA. d. melanogaster. [Q03043-1 ]

Organism-specific databases

CTDi 44817.
FlyBasei FBgn0000721. for.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074358.
InParanoidi Q03043.
KOi K07376.
OMAi QMQGLRI.
OrthoDBi EOG75B84Q.
PhylomeDBi Q03043.

Enzyme and pathway databases

BRENDAi 2.7.11.12. 1994.
Reactomei REACT_184321. Ca2+ pathway.
SignaLinki Q03043.

Miscellaneous databases

GenomeRNAii 44817.
NextBioi 837662.
PROi Q03043.

Gene expression databases

Bgeei Q03043.

Family and domain databases

Gene3Di 2.60.120.10. 2 hits.
InterProi IPR000961. AGC-kinase_C.
IPR002374. cGMP_dep_kinase.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR00104. CGMPKINASE.
SMARTi SM00100. cNMP. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cGMP-dependent protein kinase genes in Drosophila."
    Kalderon D., Rubin G.M.
    J. Biol. Chem. 264:10738-10748(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS T1 AND T3B), DEVELOPMENTAL STAGE.
    Strain: Canton-S.
    Tissue: Imaginal disk.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM T1).
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-934 AND THR-938, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiKGP24_DROME
AccessioniPrimary (citable) accession number: Q03043
Secondary accession number(s): A4V042
, Q24304, Q9I7Q1, Q9VQT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 2005
Last modified: September 3, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi