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Q03043 (KGP24_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B

Short name=cGK
EC=2.7.11.12
Alternative name(s):
Foraging protein
Gene names
Name:for
Synonyms:DG2, PGK2, Pkg24A
ORF Names:CG10033
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1088 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Developmental stage

Expressed throughout development, high during embryonic and adult stages. Isoform T1 is predominantly expressed during embryo and adult stages. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 2 cyclic nucleotide-binding domains.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandATP-binding
cGMP
cGMP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfeeding behavior

Traceable author statement PubMed 11715043. Source: FlyBase

habituation

Inferred from mutant phenotype PubMed 11040266. Source: FlyBase

larval feeding behavior

Inferred from mutant phenotype PubMed 89278147. Source: UniProtKB

larval locomotory behavior

Inferred from mutant phenotype PubMed 8506349. Source: FlyBase

long-term memory

Inferred from mutant phenotype PubMed 17640898. Source: FlyBase

protein phosphorylation

Inferred from direct assay PubMed 89278147. Source: UniProtKB

regulation of heart contraction

Inferred from mutant phenotype PubMed 11919704. Source: FlyBase

regulation of response to food

Inferred from mutant phenotype PubMed 19696884. Source: FlyBase

response to sucrose

Inferred from direct assay PubMed 17701979. Source: FlyBase

short-term memory

Inferred from mutant phenotype PubMed 17640898. Source: FlyBase

   Cellular_componentplasma membrane

Inferred from direct assay PubMed 15218025. Source: FlyBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cGMP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cGMP-dependent protein kinase activity

Inferred from direct assay PubMed 89278147. Source: UniProtKB

cyclic nucleotide-dependent protein kinase activity

Inferred from sequence or structural similarity PubMed 3215511. Source: FlyBase

protein serine/threonine kinase activity

Non-traceable author statement PubMed 10908587. Source: FlyBase

Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform T1 (identifier: Q03043-1)

Also known as: A; H;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform cD4 (identifier: Q03043-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-520: Missing.
Isoform T3A (identifier: Q03043-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.
Isoform T3B (identifier: Q03043-3)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-346: Missing.
Isoform cD5 (identifier: P32023-1)

Also known as: E; J;

The sequence of this isoform can be found in the external entry P32023.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform T2 (identifier: P32023-2)

Also known as: C; D; F; G; T2a; T2b;

The sequence of this isoform can be found in the external entry P32023.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10881088cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B
PRO_0000086121

Regions

Domain777 – 1036260Protein kinase
Domain1037 – 108852AGC-kinase C-terminal
Nucleotide binding529 – 645117cGMP 1 By similarity
Nucleotide binding646 – 761116cGMP 2 By similarity
Nucleotide binding783 – 7919ATP By similarity
Region1 – 6969Dimerization By similarity
Region70 – 528459Regulatory By similarity

Sites

Active site9011Proton acceptor By similarity
Binding site8071ATP By similarity

Amino acid modifications

Modified residue9341Phosphothreonine Ref.5
Modified residue9381Phosphothreonine Ref.5

Natural variations

Alternative sequence1 – 520520Missing in isoform cD4.
VSP_004763
Alternative sequence1 – 346346Missing in isoform T3B.
VSP_004762
Alternative sequence1 – 169169Missing in isoform T3A.
VSP_004761

Experimental info

Sequence conflict491P → T in AAA28455. Ref.1
Sequence conflict2071S → T in AAA28455. Ref.1
Sequence conflict6641Y → H in AAA28455. Ref.1
Sequence conflict6641Y → H in AAA28459. Ref.1
Sequence conflict6921T → R in AAA28455. Ref.1
Sequence conflict6921T → R in AAA28459. Ref.1
Sequence conflict9151V → G in AAA28455. Ref.1
Sequence conflict9151V → G in AAA28459. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform T1 (A) (H) [UniParc].

Last modified February 1, 2005. Version 3.
Checksum: A9450E31FF7AB8BD

FASTA1,088121,341
        10         20         30         40         50         60 
MRFCFDRLCF ATKRPAQNSN SNAPHSSTTV DAPPRPADVD VATVPVATPA PPPQQPVSNL 

        70         80         90        100        110        120 
FYADYQKLQP AIIDRDWERD RDTDTDTRSE AKPPDIVEHI EPVEEQRQIH TQIQSPAEIQ 

       130        140        150        160        170        180 
IQIPPTPPAP SIQIQIQQRY RRHSSAEDRN LNTRRNDSNI TEALRKAASM QQEPNANYQF 

       190        200        210        220        230        240 
PTDLGLVSIV NNNNNTNTHP SGSNSGSNNN SNINNNLVGG IVTLPAAGGL IGLEHTASGL 

       250        260        270        280        290        300 
RLIPAPPTHS DVLTHTLIYG TPPSGAQQLN QDPRSLLHQQ ELQLQQRYQQ LQQLQAQTQG 

       310        320        330        340        350        360 
LYTSQGSPVL YHQPSPGSSQ PVAIPGATCH SPTQLQPPNT LNLQQQMQSL RISGCTPSGT 

       370        380        390        400        410        420 
GGSATPSPVG LVDPNFIVSN YVAASPQEER FIQIIQAKEL KIQEMQRALQ FKDNEIAELK 

       430        440        450        460        470        480 
SHLDKFQSVF PFSRGSAAGC AGTGGASGSG AGGSGGSGPG TATGATRKSG QNFQRQRALG 

       490        500        510        520        530        540 
ISAEPQSESS LLLEHVSFPK YDKDERSREL IKAAILDNDF MKNLDLTQIR EIVDCMYPVK 

       550        560        570        580        590        600 
YPAKNLIIKE GDVGSIVYVM EDGRVEVSRE GKYLSTLSGA KVLGELAILY NCQRTATITA 

       610        620        630        640        650        660 
ITECNLWAIE RQCFQTIMMR TGLIRQAEYS DFLKSVPIFK DLAEDTLIKI SDVLEETHYQ 

       670        680        690        700        710        720 
RGDYIVRQGA RGDTFFIISK GKVRVTIKQQ DTQEEKFIRM LGKGDFFGEK ALQGDDLRTA 

       730        740        750        760        770        780 
NIICESADGV SCLVIDRETF NQLISNLDEI KHRYDDEGAM ERRKINEEFR DINLTDLRVI 

       790        800        810        820        830        840 
ATLGVGGFGR VELVQTNGDS SRSFALKQMK KSQIVETRQQ QHIMSEKEIM GEANCQFIVK 

       850        860        870        880        890        900 
LFKTFKDKKY LYMLMESCLG GELWTILRDK GNFDDSTTRF YTACVVEAFD YLHSRNIIYR 

       910        920        930        940        950        960 
DLKPENLLLN ERGYVKLVDF GFAKKLQTGR KTWTFCGTPE YVAPEVILNR GHDISADYWS 

       970        980        990       1000       1010       1020 
LGVLMFELLT GTPPFTGSDP MRTYNIILKG IDAIEFPRNI TRNASNLIKK LCRDNPAERL 

      1030       1040       1050       1060       1070       1080 
GYQRGGISEI QKHKWFDGFY WWGLQNCTLE PPIKPAVKSV VDTTNFDDYP PDPEGPPPDD 


VTGWDKDF 

« Hide

Isoform cD4 [UniParc].

Checksum: A5A275D0B5A50397
Show »

FASTA56865,073
Isoform T3A [UniParc].

Checksum: FD7DDDFC3391F3AD
Show »

FASTA919102,235
Isoform T3B (B) [UniParc].

Checksum: 8354AAA1630DCE09
Show »

FASTA74283,349
Isoform cD5 (E) (J) [UniParc].

See P32023.

Isoform T2 (C) (D) (F) (G) (T2a) (T2b) [UniParc].

See P32023.

References

« Hide 'large scale' references
[1]"cGMP-dependent protein kinase genes in Drosophila."
Kalderon D., Rubin G.M.
J. Biol. Chem. 264:10738-10748(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS T1 AND T3B), DEVELOPMENTAL STAGE.
Strain: Canton-S.
Tissue: Imaginal disk.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM T1).
Strain: Berkeley.
Tissue: Embryo.
[5]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-934 AND THR-938, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M27120 expand/collapse EMBL AC list , M27117, M27118, M27119 Genomic DNA. Translation: AAA28455.1.
M30413 mRNA. Translation: AAA28459.1.
AE014134 Genomic DNA. Translation: AAF51082.2.
AE014134 Genomic DNA. Translation: AAG22251.1.
AE014134 Genomic DNA. Translation: AAS64613.1.
AE014134 Genomic DNA. Translation: AAS64614.1.
AY061514 mRNA. Translation: AAL29062.1.
PIRB34106.
RefSeqNP_477487.1. NM_058139.3.
NP_477488.1. NM_058140.3.
NP_995626.1. NM_205904.1.
NP_995628.1. NM_205906.1.
UniGeneDm.6584.

3D structure databases

ProteinModelPortalQ03043.
SMRQ03043. Positions 475-1074.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid69303. 9 interactions.
MINTMINT-795797.

Proteomic databases

PaxDbQ03043.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0089304; FBpp0088350; FBgn0000721. [Q03043-1]
FBtr0089310; FBpp0088922; FBgn0000721. [Q03043-1]
FBtr0089312; FBpp0088923; FBgn0000721. [Q03043-1]
GeneID44817.
KEGGdme:Dmel_CG10033.
UCSCCG10033-RA. d. melanogaster. [Q03043-1]

Organism-specific databases

CTD44817.
FlyBaseFBgn0000721. for.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000074358.
InParanoidQ03043.
KOK07376.
OMAQMQGLRI.
OrthoDBEOG75B84Q.
PhylomeDBQ03043.

Enzyme and pathway databases

BRENDA2.7.11.12. 1994.
SignaLinkQ03043.

Gene expression databases

BgeeQ03043.

Family and domain databases

Gene3D2.60.120.10. 2 hits.
InterProIPR000961. AGC-kinase_C.
IPR002374. cGMP_dep_kinase.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR00104. CGMPKINASE.
SMARTSM00100. cNMP. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi44817.
NextBio837662.
PROQ03043.

Entry information

Entry nameKGP24_DROME
AccessionPrimary (citable) accession number: Q03043
Secondary accession number(s): A4V042 expand/collapse secondary AC list , Q24304, Q9I7Q1, Q9VQT2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 2005
Last modified: April 16, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase