Q03042 (KGP1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cGMP-dependent protein kinase, isozyme 1
Short name=cGK
EC=2.7.11.12

Gene names

Name:	Pkg21D
Synonyms:	DG1
ORF Names:	CG3324

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	768 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium.
Enzyme regulation	Binding of cGMP results in enzyme activation.
Subunit structure	Homodimer. Ref.2
Tissue specificity	In embryo stage 13, expression is seen in a few large, irregular cells having the appearance of hemocytes or macrophages. In adults, expression is seen in optic lamina and weakly in testis. Ref.2
Developmental stage	Highest expression is in embryos, low level expression is seen through rest of development. Ref.1 Ref.2
Post-translational modification	Autophosphorylated. Ref.2
Miscellaneous	cGMP-dependent protein kinase 1 consists of 3 types of domains: the regulatory domain, two cGMP-binding regions and the catalytic domain By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 cyclic nucleotide-binding domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding cGMP cGMP-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP-dependent protein kinase activity Inferred from direct assay Ref.2. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 768	768	cGMP-dependent protein kinase, isozyme 1		PRO_0000086120
Regions
	Domain	457 – 717	261	Protein kinase
	Domain	718 – 768	51	AGC-kinase C-terminal
	Nucleotide binding	185 – 301	117	cGMP 1
	Nucleotide binding	304 – 427	124	cGMP 2
	Nucleotide binding	463 – 471	9	ATP By similarity
	Region	1 – 192	192	Regulatory By similarity
Sites
	Active site	582	1	Proton acceptor By similarity
	Binding site	488	1	ATP By similarity
Experimental info
	Sequence conflict	235	1	Q → H in AAB03405. Ref.2
	Sequence conflict	434	1	Q → R in AAB03405. Ref.2
	Sequence conflict	438	1	R → Q in AAB03405. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q03042 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: 36A97452319BE63C	FASTA	768	86,759
	10 20 30 40 50 60 MAAGMLTDRE REAIVSNLTK DVQALREMVR SRESELVKLH REIHKLKSVL QQTTNNLNVT 70 80 90 100 110 120 RNEKAKKKLY SLPEQCGEQE SRNQNPHLCS SCGMVLPTSP EFALEALSLG PLSPLASTSS 130 140 150 160 170 180 ASPSGRTSAD EVRPKAMPAA IKKQGVSAES CVQSMQQSYS IPIPKYEKDF SDKQQIKDAI 190 200 210 220 230 240 MDNDFLKNID ASQVRELVDS MYSKSIAAGE FVIREGEVGA HLYVSAAGEF AVMQQGKVLD 250 260 270 280 290 300 KMGAGKAFGE LAILYNCTRT ASIRVLSEAA RVWVLDRRVF QQIMMCTGLQ RIENSVNFLR 310 320 330 340 350 360 SVPLLMNLSE ELLAKIADVL ELEFYAAGTY IIRQGTAGDS FFLISQGNVR VTQKLTPTSP 370 380 390 400 410 420 EETELRTLSR GDYFGEQALI NEDKRTANII ALSPGVECLT LDRDSFKRLI GDLCELKEKD 430 440 450 460 470 480 YGDESRKLAM KQAQESCRDE PKEQLQQEFP DLKLTDLEVV STLGIGGFGR VELVKAHHQD 490 500 510 520 530 540 RVDIFALKCL KKRHIVDTKQ EEHIFSERHI MLSSRSPFIC RLYRTFRDEK YVYMLLEACM 550 560 570 580 590 600 GGEIWTMLRD RGSFEDNAAQ FIIGCVLQAF EYLHARGIIY RDLKPENLML DERGYVKIVD 610 620 630 640 650 660 FGFAKQIGTS SKTWTFCGTP EYVAPEIILN KGHDRAVDYW ALGILIHELL NGTPPFSAPD 670 680 690 700 710 720 PMQTYNLILK GIDMIAFPKH ISRWAVQLIK RLCRDVPSER LGYQTGGIQD IKKHKWFLGF 730 740 750 760 DWDGLASQLL IPPFVRPIAH PTDVRYFDRF PCDLNEPPDE LSGWDADF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cGMP-dependent protein kinase genes in Drosophila." Kalderon D., Rubin G.M. J. Biol. Chem. 264:10738-10748(1989) [PubMed: 2732245] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE. Tissue: Larva.
[2]	"Biochemical properties and cellular localization of the Drosophila DG1 cGMP-dependent protein kinase." Foster J.L., Higgins G.C., Jackson F.R. J. Biol. Chem. 271:23322-23328(1996) [PubMed: 8798533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, AUTOPHOSPHORYLATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[6]	"Cloning, sequence, and expression of the Drosophila cAMP-dependent protein kinase catalytic subunit gene." Foster J.L., Higgins G.C., Jackson R.F. J. Biol. Chem. 263:1676-1681(1988) [PubMed: 2828348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 172-644.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27114, M27113 Genomic DNA. Translation: AAA28453.1. U59901 mRNA. Translation: AAB03405.1. AE014134 Genomic DNA. Translation: AAF51459.1. AY058288 mRNA. Translation: AAL13517.1.
PIR	A34106.
RefSeq	NP_477213.1. NM_057865.3.
UniGene	Dm.4323.
3D structure databases
ProteinModelPortal	Q03042.
SMR	Q03042. Positions 162-430, 450-768.
ModBase	Search...
Protein-protein interaction databases
STRING	Q03042.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0078042; FBpp0077706; FBgn0000442.
GeneID	33253.
KEGG	dme:Dmel_CG3324.
NMPDR	fig|7227.3.peg.142.
Organism-specific databases
CTD	33253.
FlyBase	FBgn0000442. Pkg21D.
Phylogenomic databases
eggNOG	inNOG06017.
GeneTree	EMGT00050000000291.
InParanoid	Q03042.
OMA	RWAVQLI.
OrthoDB	EOG4D254S.
PhylomeDB	Q03042.
Enzyme and pathway databases
BRENDA	2.7.11.12. 1994.
Gene expression databases
ArrayExpress	Q03042.
Bgee	Q03042.
GermOnline	CG3324. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR016232. cGMP-dependent_protein_kinase. IPR002374. cGMP_dep_kinase. IPR018490. cNMP-bd-like. IPR018488. cNMP-bd_CS. IPR000595. cNMP-bd_dom. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR014710. RmlC-like_jellyroll. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
KO	K07376.
Pfam	PF00027. cNMP_binding. 2 hits. PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF000559. cGMP-dep_kinase. 1 hit.
PRINTS	PR00104. CGMPKINASE.
SMART	SM00100. cNMP. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF51206. cNMP_binding. 2 hits. SSF56112. Kinase_like. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00888. CNMP_BINDING_1. 1 hit. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	782672.

Entry information

Entry name

KGP1_DROME

Accession

Primary (citable) accession number: Q03042
Secondary accession number(s): Q24566, Q9V403

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 2, 2001
Last modified:	January 25, 2012
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project