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Protein

Cyclic nucleotide-gated olfactory channel

Gene

CNGA2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Odorant signal transduction is probably mediated by a G-protein coupled cascade using cAMP as second messenger. The olfactory channel can be shown to be activated by cyclic nucleotides which leads to a depolarization of olfactory sensory neurons.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei521 – 5211cAMPBy similarity
Binding sitei536 – 5361cAMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi462 – 584123cAMPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel

Keywords - Biological processi

Ion transport, Olfaction, Sensory transduction, Transport

Keywords - Ligandi

Calmodulin-binding, cAMP, cAMP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic nucleotide-gated olfactory channel
Alternative name(s):
Cyclic nucleotide-gated cation channel 2
Cyclic nucleotide-gated channel alpha-2
Short name:
CNG channel alpha-2
Short name:
CNG-2
Short name:
CNG2
Gene namesi
Name:CNGA2
Synonyms:CNCG2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 140140CytoplasmicSequence analysisAdd
BLAST
Transmembranei141 – 16020Helical; Name=H1Sequence analysisAdd
BLAST
Topological domaini161 – 17313ExtracellularSequence analysisAdd
BLAST
Transmembranei174 – 19219Helical; Name=H2Sequence analysisAdd
BLAST
Topological domaini193 – 21624CytoplasmicSequence analysisAdd
BLAST
Transmembranei217 – 23620Helical; Name=H3Sequence analysisAdd
BLAST
Topological domaini237 – 27438ExtracellularSequence analysisAdd
BLAST
Transmembranei275 – 29723Helical; Name=H4Sequence analysisAdd
BLAST
Topological domaini298 – 34952CytoplasmicSequence analysisAdd
BLAST
Transmembranei350 – 36920Helical; Name=H5Sequence analysisAdd
BLAST
Topological domaini370 – 45384ExtracellularSequence analysisAdd
BLAST
Transmembranei454 – 47421Helical; Name=H6Sequence analysisAdd
BLAST
Topological domaini475 – 663189CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 663663Cyclic nucleotide-gated olfactory channelPRO_0000219311Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi379 – 3791N-linked (GlcNAc...)Curated

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ03041.
PRIDEiQ03041.

Expressioni

Tissue specificityi

Olfactory neurons.

Interactioni

Subunit structurei

Heterotetramer composed of two subunits of CNGA2, one of CNGA4 and one of CNGB1b. The complex forms the cyclic nucleotide-gated (CNG) channel of olfactory sensory neurons (By similarity).By similarity

Protein-protein interaction databases

IntActiQ03041. 1 interaction.
MINTiMINT-7032993.
STRINGi9913.ENSBTAP00000027618.

Structurei

Secondary structure

1
663
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi66 – 8116Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SY9NMR-B60-85[»]
ProteinModelPortaliQ03041.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03041.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili597 – 64044By similarityAdd
BLAST

Domaini

The C-terminal coiled-coil domain mediates trimerization of CNGA subunits.By similarity

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0500. Eukaryota.
ENOG410YWWI. LUCA.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiQ03041.
KOiK04949.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032406. CLZ_dom.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF16526. CLZ. 1 hit.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEKANGVKS SPANNHNHHA PPAIKASGKD DHRASSRPQS AAADDTSSEL
60 70 80 90 100
QQLAEMDAPQ QRRGGFRRIA RLVGVLREWA YRNFREEEPR PDSFLERFRG
110 120 130 140 150
PELHTVTTQQ GDGKGDKDGE GKGTKKKFEL FVLDPAGDWY YRWLFLIALP
160 170 180 190 200
VLYNWCLLVA RACFSDLQKG YYIVWLVLDY VSDVVYIADL FIRLRTGFLE
210 220 230 240 250
QGLLVKDTKK LRDNYIHTMQ FKLDVASIIP TDLIYFAVGI HNPEVRFNRL
260 270 280 290 300
LHFARMFEFF DRTETRTSYP NIFRISNLIL YILIIIHWNA CIYYAISKSI
310 320 330 340 350
GFGVDTWVYP NITDPEYGYL SREYIYCLYW STLTLTTIGE TPPPVKDEEY
360 370 380 390 400
LFVIFDFLIG VLIFATIVGN VGSMISNMNA TRAEFQAKID AVKHYMQFRK
410 420 430 440 450
VSKEMEAKVI RWFDYLWTNK KSVDEREVLK NLPAKLRAEI AINVHLSTLK
460 470 480 490 500
KVRIFQDCEA GLLVELVLKL RPQVFSPGDY ICRKGDIGKE MYIIKEGKLA
510 520 530 540 550
VVADDGVTQY ALLSAGSCFG EISILNIKGS KMGNRRTANI RSLGYSDLFC
560 570 580 590 600
LSKDDLMEAV TEYPDAKRVL EERGREILMK EGLLDENEVA ASMEVDVQEK
610 620 630 640 650
LEQLETNMDT LYTRFARLLA EYTGAQQKLK QRITVLETKM KQNNEDDSLS
660
DGMNSPEPPA EKP
Length:663
Mass (Da):76,014
Last modified:July 1, 1993 - v1
Checksum:iAB66D4F9203844EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55010 mRNA. Translation: CAA38754.1.
PIRiS11521.
RefSeqiNP_001001139.1. NM_001001139.2.
UniGeneiBt.28014.

Genome annotation databases

GeneIDi407172.
KEGGibta:407172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55010 mRNA. Translation: CAA38754.1.
PIRiS11521.
RefSeqiNP_001001139.1. NM_001001139.2.
UniGeneiBt.28014.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SY9NMR-B60-85[»]
ProteinModelPortaliQ03041.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ03041. 1 interaction.
MINTiMINT-7032993.
STRINGi9913.ENSBTAP00000027618.

Proteomic databases

PaxDbiQ03041.
PRIDEiQ03041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi407172.
KEGGibta:407172.

Organism-specific databases

CTDi1260.

Phylogenomic databases

eggNOGiKOG0500. Eukaryota.
ENOG410YWWI. LUCA.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiQ03041.
KOiK04949.

Miscellaneous databases

EvolutionaryTraceiQ03041.
NextBioi20818428.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032406. CLZ_dom.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF16526. CLZ. 1 hit.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of cAMP-gated channel from bovine olfactory epithelium."
    Ludwig J., Margalit T., Eismann E., Lancet D., Kaupp U.B.
    FEBS Lett. 270:24-29(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Olfactory epithelium.
  2. "Structure of calmodulin complexed with an olfactory CNG channel fragment and role of the central linker: residual dipolar couplings to evaluate calmodulin binding modes outside the kinase family."
    Contessa G.M., Orsale M., Melino S., Torre V., Paci M., Desideri A., Cicero D.O.
    J. Biomol. NMR 31:185-199(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 60-85 IN COMPLEX WITH CALMODULIN.

Entry informationi

Entry nameiCNGA2_BOVIN
AccessioniPrimary (citable) accession number: Q03041
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 11, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The olfactory channel is activated by both cAMP and cGMP at similar concentrations, whereas the cGMP-gated channel is much less sensitive to cAMP.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.