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Protein

Proteinase inhibitor

Gene

inh

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of the alkaline protease. It forms a non-covalent bond with the protease and may prevent its autocatalytic cleavage in the periplasm (By similarity).By similarity

GO - Molecular functioni

  1. metalloendopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor

Protein family/group databases

MEROPSiI38.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinase inhibitor
Alternative name(s):
Aprin
Gene namesi
Name:inh
Synonyms:aprI
Ordered Locus Names:PA1250
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1250.

Subcellular locationi

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626By similarityAdd
BLAST
Chaini27 – 131105Proteinase inhibitorPRO_0000026717Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi51 ↔ 74By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

MINTiMINT-204143.
STRINGi208964.PA1250.

Structurei

Secondary structure

1
131
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi33 – 364Combined sources
Beta strandi38 – 447Combined sources
Beta strandi50 – 6011Combined sources
Turni61 – 644Combined sources
Beta strandi65 – 717Combined sources
Helixi72 – 776Combined sources
Beta strandi79 – 813Combined sources
Beta strandi85 – 895Combined sources
Beta strandi92 – 965Combined sources
Beta strandi98 – 1003Combined sources
Beta strandi102 – 1109Combined sources
Beta strandi113 – 1175Combined sources
Beta strandi119 – 1213Combined sources
Beta strandi123 – 1286Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JIWX-ray1.74I26-131[»]
2RN4NMR-A26-131[»]
SMRiQ03026. Positions 26-131.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03026.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG47174.
HOGENOMiHOG000247112.
OMAiPAGWRPT.
OrthoDBiEOG69SKD8.

Family and domain databases

Gene3Di2.40.128.10. 1 hit.
InterProiIPR021140. Inh/Omp19.
IPR003086. MPTase_inhib_I38.
IPR022815. Prot_inh_I38.
IPR016085. Protease_inh_b-brl_dom.
[Graphical view]
PfamiPF02974. Inh. 1 hit.
[Graphical view]
PRINTSiPR01274. MPTASEINHBTR.
ProDomiPD015804. MPTase_inhib_I38. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50882. SSF50882. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASAKLSRM VCLLCGFFST GISMASSLIL LSASDLAGQW TLQQDEAPAI
60 70 80 90 100
CHLELRDSEV AEASGYDLGG DTACLTRWLP SEPRAWRPTP AGIALLERGG
110 120 130
LTLMLLGRQG EGDYRVQKGD GGQLVLRRAT P
Length:131
Mass (Da):13,980
Last modified:October 1, 1993 - v1
Checksum:i8CE4A64ED10AA8CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64558 Genomic DNA. Translation: CAA45859.1.
AE004091 Genomic DNA. Translation: AAG04639.1.
PIRiS26700.
RefSeqiNP_249941.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04639; AAG04639; PA1250.
GeneIDi881261.
KEGGipae:PA1250.
PATRICi19836824. VBIPseAer58763_1298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64558 Genomic DNA. Translation: CAA45859.1.
AE004091 Genomic DNA. Translation: AAG04639.1.
PIRiS26700.
RefSeqiNP_249941.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JIWX-ray1.74I26-131[»]
2RN4NMR-A26-131[»]
SMRiQ03026. Positions 26-131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-204143.
STRINGi208964.PA1250.

Protein family/group databases

MEROPSiI38.002.

Protocols and materials databases

DNASUi881261.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04639; AAG04639; PA1250.
GeneIDi881261.
KEGGipae:PA1250.
PATRICi19836824. VBIPseAer58763_1298.

Organism-specific databases

PseudoCAPiPA1250.

Phylogenomic databases

eggNOGiNOG47174.
HOGENOMiHOG000247112.
OMAiPAGWRPT.
OrthoDBiEOG69SKD8.

Miscellaneous databases

EvolutionaryTraceiQ03026.

Family and domain databases

Gene3Di2.40.128.10. 1 hit.
InterProiIPR021140. Inh/Omp19.
IPR003086. MPTase_inhib_I38.
IPR022815. Prot_inh_I38.
IPR016085. Protease_inh_b-brl_dom.
[Graphical view]
PfamiPF02974. Inh. 1 hit.
[Graphical view]
PRINTSiPR01274. MPTASEINHBTR.
ProDomiPD015804. MPTase_inhib_I38. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50882. SSF50882. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways."
    Duong F., Lazdunski A., Cami B., Murgier M.
    Gene 121:47-54(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. "Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond."
    Hege T., Feltzer R.E., Gray R.D., Baumann U.
    J. Biol. Chem. 276:35087-35092(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS) IN COMPLEX WITH PROTEASE.

Entry informationi

Entry nameiINH_PSEAE
AccessioniPrimary (citable) accession number: Q03026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 1, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.