Q03019 (TWINE_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cdc25-like protein phosphatase twine
EC=3.1.3.48

Gene names

Name:	twe
ORF Names:	CG4965

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required during meiosis. Regulates the transition from the extended G2 phase to the onset of the first meiotic division.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Tissue specificity	Expressed in developing male and female germ cells.
Sequence similarities	Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	M phase of mitotic cell cycle Inferred from electronic annotation. Source: InterPro cell division Inferred from electronic annotation. Source: UniProtKB-KW embryo development Inferred from mutant phenotype PubMed 2492966. Source: FlyBase male meiosis Inferred from mutant phenotype Ref.1. Source: FlyBase meiotic G2/MI transition Traceable author statement PubMed 9813190. Source: FlyBase meiotic nuclear envelope disassembly Inferred from mutant phenotype PubMed 18052611. Source: FlyBase pole cell formation Inferred from mutant phenotype PubMed 2492966. Source: FlyBase regulation of meiotic cell cycle Inferred from mutant phenotype PubMed 18927152. Source: FlyBase regulation of syncytial blastoderm mitotic cell cycle Inferred from mutant phenotype Ref.1. Source: FlyBase spermatogenesis Inferred from mutant phenotype PubMed 10559904. Source: FlyBase
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	protein tyrosine phosphatase activity Inferred from sequence or structural similarity Ref.2Ref.6Ref.1. Source: FlyBase protein tyrosine/serine/threonine phosphatase activity Non-traceable author statement PubMed 10908587. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
cdc2	P23572	4	EBI-138996,EBI-108689

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Cdc25-like protein phosphatase twine		PRO_0000198659
Regions
	Domain	265 – 371	107	Rhodanese
Sites
	Active site	318	1	By similarity
Experimental info
	Sequence conflict	48	1	L → M in CAA48783. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q03019 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 0F8626692B683D08	FASTA	426	48,320
	10 20 30 40 50 60 MASKRLMLDV EEEDDESGAC GQENFDPHDA DMEYQAKRRK SAVQETPLQW MLKRHIPAST 70 80 90 100 110 120 TVLSPITELS QNMNGARLDG TPKSTQRIPA NRTLNNFNSL SSRTLGSFSS SCSSYESGNS 130 140 150 160 170 180 LDDEYMDMFE MESAENHNLE LPDDLEVLLS GQLKSESNLE EMSNKKGSLR RCLSMYPSEQ 190 200 210 220 230 240 PEEAVQEPDQ ETNMPMKKMQ RKTLSMNDAE IMRALGDEPE LIGDLSKPCT LPCLATGIRH 250 260 270 280 290 300 RDLKTISSDT LARLIQGEFD EQLGSQGGYE IIDCRYPYEF LGGHIRGAKN LYTRGQIQEA 310 320 330 340 350 360 FPTLTSNQEN RRIYVFHCEF SSERGPKLLR YLRSNDRSQH THNYPALDYP ELYILHNGYK 370 380 390 400 410 420 EFFGLYSQLC QPSQYVPMLA PAHNDEFRYF RAKTKSWQCG EGGDSGIGGG GSRGLRKSRS RLLYAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Twine, a cdc25 homolog that functions in the male and female germline of Drosophila." Alphey L.S., Jimenez J., White-Cooper H., Dawson I., Nurse P., Glover D.M. Cell 69:977-988(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region." Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T., Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G., Harvey D.A., Hong L., Houston K.A., Hoskins R.A., Johnson G., Martin C., Moshrefi A.R. , Palazzolo M., Reese M.G., Spradling A.C., Tsang G., Wan K.H., Whitelaw K., Celniker S.E., Rubin G.M. Genetics 153:179-219(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"The Drosophila cdc25 homolog twine is required for meiosis." Courtot C., Fankhauser C., Simanis V., Lehner C.F. Development 116:405-416(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-426.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M94158 mRNA. Translation: AAA28413.1. AE014134 Genomic DNA. Translation: AAF53508.1. AY061266 mRNA. Translation: AAL28814.1. X69018 mRNA. Translation: CAA48783.1.
PIR	A41910.
RefSeq	NP_001260491.1. NM_001273562.1. NP_001260492.1. NM_001273563.1. NP_001260493.1. NM_001273564.1. NP_476633.1. NM_057285.4.
UniGene	Dm.3667.
3D structure databases
ProteinModelPortal	Q03019.
SMR	Q03019. Positions 221-387.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-23538N.
IntAct	Q03019. 2 interactions.
MINT	MINT-942016.
STRING	7227.FBpp0080412.
Proteomic databases
PaxDb	Q03019.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0080855; FBpp0080412; FBgn0002673. FBtr0331444; FBpp0303861; FBgn0002673. FBtr0331445; FBpp0303862; FBgn0002673. FBtr0331446; FBpp0303863; FBgn0002673.
GeneID	34954.
KEGG	dme:Dmel_CG4965.
UCSC	CG4965-RA. d. melanogaster.
Organism-specific databases
CTD	34954.
FlyBase	FBgn0002673. twe.
Phylogenomic databases
eggNOG	COG5105.
GeneTree	ENSGT00390000018747.
InParanoid	Q03019.
OMA	RAKTKSW.
OrthoDB	EOG4RBP0Z.
PhylomeDB	Q03019.
Gene expression databases
Bgee	Q03019.
GermOnline	CG4965. Drosophila melanogaster.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR000751. MPI_Phosphatase. IPR001763. Rhodanese-like_dom. [Graphical view]
PANTHER	PTHR10828. PTHR10828. 1 hit.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
PRINTS	PR00716. MPIPHPHTASE.
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	34954.
NextBio	791075.

Entry information

Entry name

TWINE_DROME

Accession

Primary (citable) accession number: Q03019
Secondary accession number(s): Q9V454

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project