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Q03019

- TWINE_DROME

UniProt

Q03019 - TWINE_DROME

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Protein

Cdc25-like protein phosphatase twine

Gene

twe

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required during meiosis. Regulates the transition from the extended G2 phase to the onset of the first meiotic division.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei318 – 3181By similarity

GO - Molecular functioni

  1. protein tyrosine/serine/threonine phosphatase activity Source: FlyBase
  2. protein tyrosine phosphatase activity Source: FlyBase

GO - Biological processi

  1. cellularization Source: FlyBase
  2. male meiosis Source: FlyBase
  3. meiotic G2/MI transition Source: FlyBase
  4. meiotic nuclear envelope disassembly Source: FlyBase
  5. peptidyl-tyrosine dephosphorylation Source: GOC
  6. pole cell formation Source: FlyBase
  7. protein dephosphorylation Source: FlyBase
  8. regulation of meiotic cell cycle Source: FlyBase
  9. regulation of syncytial blastoderm mitotic cell cycle Source: FlyBase
  10. spermatogenesis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Meiosis

Enzyme and pathway databases

ReactomeiREACT_180658. G0 and Early G1.
REACT_208929. Polo-like kinase mediated events.
REACT_218568. Activation of ATR in response to replication stress.
REACT_222733. E2F-enabled inhibition of pre-replication complex formation.
REACT_222970. p53-Independent DNA Damage Response.
REACT_225636. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_225883. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Names & Taxonomyi

Protein namesi
Recommended name:
Cdc25-like protein phosphatase twine (EC:3.1.3.48)
Gene namesi
Name:twe
ORF Names:CG4965
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0002673. twe.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426Cdc25-like protein phosphatase twinePRO_0000198659Add
BLAST

Proteomic databases

PaxDbiQ03019.

Expressioni

Tissue specificityi

Expressed in developing male and female germ cells.

Gene expression databases

BgeeiQ03019.
ExpressionAtlasiQ03019. differential.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
cdc2P235724EBI-138996,EBI-108689

Protein-protein interaction databases

BioGridi60966. 6 interactions.
DIPiDIP-23538N.
IntActiQ03019. 2 interactions.
MINTiMINT-942016.
STRINGi7227.FBpp0080412.

Structurei

3D structure databases

ProteinModelPortaliQ03019.
SMRiQ03019. Positions 221-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini265 – 371107RhodanesePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5105.
GeneTreeiENSGT00390000018747.
InParanoidiQ03019.
OMAiRAKTKSW.
OrthoDBiEOG757D02.
PhylomeDBiQ03019.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03019 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASKRLMLDV EEEDDESGAC GQENFDPHDA DMEYQAKRRK SAVQETPLQW
60 70 80 90 100
MLKRHIPAST TVLSPITELS QNMNGARLDG TPKSTQRIPA NRTLNNFNSL
110 120 130 140 150
SSRTLGSFSS SCSSYESGNS LDDEYMDMFE MESAENHNLE LPDDLEVLLS
160 170 180 190 200
GQLKSESNLE EMSNKKGSLR RCLSMYPSEQ PEEAVQEPDQ ETNMPMKKMQ
210 220 230 240 250
RKTLSMNDAE IMRALGDEPE LIGDLSKPCT LPCLATGIRH RDLKTISSDT
260 270 280 290 300
LARLIQGEFD EQLGSQGGYE IIDCRYPYEF LGGHIRGAKN LYTRGQIQEA
310 320 330 340 350
FPTLTSNQEN RRIYVFHCEF SSERGPKLLR YLRSNDRSQH THNYPALDYP
360 370 380 390 400
ELYILHNGYK EFFGLYSQLC QPSQYVPMLA PAHNDEFRYF RAKTKSWQCG
410 420
EGGDSGIGGG GSRGLRKSRS RLLYAE
Length:426
Mass (Da):48,320
Last modified:October 1, 1996 - v2
Checksum:i0F8626692B683D08
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481L → M in CAA48783. (PubMed:1286615)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M94158 mRNA. Translation: AAA28413.1.
AE014134 Genomic DNA. Translation: AAF53508.1.
AY061266 mRNA. Translation: AAL28814.1.
X69018 mRNA. Translation: CAA48783.1.
PIRiA41910.
RefSeqiNP_001260491.1. NM_001273562.2.
NP_001260492.1. NM_001273563.1.
NP_001260493.1. NM_001273564.2.
NP_476633.1. NM_057285.4.
UniGeneiDm.3667.

Genome annotation databases

EnsemblMetazoaiFBtr0080855; FBpp0080412; FBgn0002673.
FBtr0331444; FBpp0303861; FBgn0002673.
FBtr0331445; FBpp0303862; FBgn0002673.
FBtr0331446; FBpp0303863; FBgn0002673.
GeneIDi34954.
KEGGidme:Dmel_CG4965.
UCSCiCG4965-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M94158 mRNA. Translation: AAA28413.1 .
AE014134 Genomic DNA. Translation: AAF53508.1 .
AY061266 mRNA. Translation: AAL28814.1 .
X69018 mRNA. Translation: CAA48783.1 .
PIRi A41910.
RefSeqi NP_001260491.1. NM_001273562.2.
NP_001260492.1. NM_001273563.1.
NP_001260493.1. NM_001273564.2.
NP_476633.1. NM_057285.4.
UniGenei Dm.3667.

3D structure databases

ProteinModelPortali Q03019.
SMRi Q03019. Positions 221-387.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 60966. 6 interactions.
DIPi DIP-23538N.
IntActi Q03019. 2 interactions.
MINTi MINT-942016.
STRINGi 7227.FBpp0080412.

Proteomic databases

PaxDbi Q03019.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0080855 ; FBpp0080412 ; FBgn0002673 .
FBtr0331444 ; FBpp0303861 ; FBgn0002673 .
FBtr0331445 ; FBpp0303862 ; FBgn0002673 .
FBtr0331446 ; FBpp0303863 ; FBgn0002673 .
GeneIDi 34954.
KEGGi dme:Dmel_CG4965.
UCSCi CG4965-RA. d. melanogaster.

Organism-specific databases

CTDi 34954.
FlyBasei FBgn0002673. twe.

Phylogenomic databases

eggNOGi COG5105.
GeneTreei ENSGT00390000018747.
InParanoidi Q03019.
OMAi RAKTKSW.
OrthoDBi EOG757D02.
PhylomeDBi Q03019.

Enzyme and pathway databases

Reactomei REACT_180658. G0 and Early G1.
REACT_208929. Polo-like kinase mediated events.
REACT_218568. Activation of ATR in response to replication stress.
REACT_222733. E2F-enabled inhibition of pre-replication complex formation.
REACT_222970. p53-Independent DNA Damage Response.
REACT_225636. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_225883. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Miscellaneous databases

GenomeRNAii 34954.
NextBioi 791075.

Gene expression databases

Bgeei Q03019.
ExpressionAtlasi Q03019. differential.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view ]
Pfami PF00581. Rhodanese. 1 hit.
[Graphical view ]
PRINTSi PR00716. MPIPHPHTASE.
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Twine, a cdc25 homolog that functions in the male and female germline of Drosophila."
    Alphey L.S., Jimenez J., White-Cooper H., Dawson I., Nurse P., Glover D.M.
    Cell 69:977-988(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region."
    Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T., Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G., Harvey D.A., Hong L., Houston K.A., Hoskins R.A., Johnson G., Martin C., Moshrefi A.R.
    , Palazzolo M., Reese M.G., Spradling A.C., Tsang G., Wan K.H., Whitelaw K., Celniker S.E., Rubin G.M.
    Genetics 153:179-219(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  6. "The Drosophila cdc25 homolog twine is required for meiosis."
    Courtot C., Fankhauser C., Simanis V., Lehner C.F.
    Development 116:405-416(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-426.

Entry informationi

Entry nameiTWINE_DROME
AccessioniPrimary (citable) accession number: Q03019
Secondary accession number(s): Q9V454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3