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Q03019

- TWINE_DROME

UniProt

Q03019 - TWINE_DROME

Protein

Cdc25-like protein phosphatase twine

Gene

twe

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Required during meiosis. Regulates the transition from the extended G2 phase to the onset of the first meiotic division.

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei318 – 3181By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein tyrosine/serine/threonine phosphatase activity Source: FlyBase
    3. protein tyrosine phosphatase activity Source: FlyBase

    GO - Biological processi

    1. cellularization Source: FlyBase
    2. male meiosis Source: FlyBase
    3. meiotic G2/MI transition Source: FlyBase
    4. meiotic nuclear envelope disassembly Source: FlyBase
    5. peptidyl-tyrosine dephosphorylation Source: GOC
    6. pole cell formation Source: FlyBase
    7. protein dephosphorylation Source: FlyBase
    8. regulation of meiotic cell cycle Source: FlyBase
    9. regulation of syncytial blastoderm mitotic cell cycle Source: FlyBase
    10. spermatogenesis Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Meiosis

    Enzyme and pathway databases

    ReactomeiREACT_180658. G0 and Early G1.
    REACT_208929. Polo-like kinase mediated events.
    REACT_218568. Activation of ATR in response to replication stress.
    REACT_222733. E2F-enabled inhibition of pre-replication complex formation.
    REACT_222970. p53-Independent DNA Damage Response.
    REACT_225636. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_225883. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cdc25-like protein phosphatase twine (EC:3.1.3.48)
    Gene namesi
    Name:twe
    ORF Names:CG4965
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0002673. twe.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 426426Cdc25-like protein phosphatase twinePRO_0000198659Add
    BLAST

    Proteomic databases

    PaxDbiQ03019.

    Expressioni

    Tissue specificityi

    Expressed in developing male and female germ cells.

    Gene expression databases

    BgeeiQ03019.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    cdc2P235724EBI-138996,EBI-108689

    Protein-protein interaction databases

    BioGridi60966. 6 interactions.
    DIPiDIP-23538N.
    IntActiQ03019. 2 interactions.
    MINTiMINT-942016.
    STRINGi7227.FBpp0080412.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03019.
    SMRiQ03019. Positions 221-387.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini265 – 371107RhodanesePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MPI phosphatase family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5105.
    GeneTreeiENSGT00390000018747.
    InParanoidiQ03019.
    OMAiRAKTKSW.
    OrthoDBiEOG757D02.
    PhylomeDBiQ03019.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    [Graphical view]
    PRINTSiPR00716. MPIPHPHTASE.
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03019-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASKRLMLDV EEEDDESGAC GQENFDPHDA DMEYQAKRRK SAVQETPLQW    50
    MLKRHIPAST TVLSPITELS QNMNGARLDG TPKSTQRIPA NRTLNNFNSL 100
    SSRTLGSFSS SCSSYESGNS LDDEYMDMFE MESAENHNLE LPDDLEVLLS 150
    GQLKSESNLE EMSNKKGSLR RCLSMYPSEQ PEEAVQEPDQ ETNMPMKKMQ 200
    RKTLSMNDAE IMRALGDEPE LIGDLSKPCT LPCLATGIRH RDLKTISSDT 250
    LARLIQGEFD EQLGSQGGYE IIDCRYPYEF LGGHIRGAKN LYTRGQIQEA 300
    FPTLTSNQEN RRIYVFHCEF SSERGPKLLR YLRSNDRSQH THNYPALDYP 350
    ELYILHNGYK EFFGLYSQLC QPSQYVPMLA PAHNDEFRYF RAKTKSWQCG 400
    EGGDSGIGGG GSRGLRKSRS RLLYAE 426
    Length:426
    Mass (Da):48,320
    Last modified:October 1, 1996 - v2
    Checksum:i0F8626692B683D08
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti48 – 481L → M in CAA48783. (PubMed:1286615)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94158 mRNA. Translation: AAA28413.1.
    AE014134 Genomic DNA. Translation: AAF53508.1.
    AY061266 mRNA. Translation: AAL28814.1.
    X69018 mRNA. Translation: CAA48783.1.
    PIRiA41910.
    RefSeqiNP_001260491.1. NM_001273562.1.
    NP_001260492.1. NM_001273563.1.
    NP_001260493.1. NM_001273564.1.
    NP_476633.1. NM_057285.4.
    UniGeneiDm.3667.

    Genome annotation databases

    EnsemblMetazoaiFBtr0080855; FBpp0080412; FBgn0002673.
    FBtr0331444; FBpp0303861; FBgn0002673.
    FBtr0331445; FBpp0303862; FBgn0002673.
    FBtr0331446; FBpp0303863; FBgn0002673.
    GeneIDi34954.
    KEGGidme:Dmel_CG4965.
    UCSCiCG4965-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94158 mRNA. Translation: AAA28413.1 .
    AE014134 Genomic DNA. Translation: AAF53508.1 .
    AY061266 mRNA. Translation: AAL28814.1 .
    X69018 mRNA. Translation: CAA48783.1 .
    PIRi A41910.
    RefSeqi NP_001260491.1. NM_001273562.1.
    NP_001260492.1. NM_001273563.1.
    NP_001260493.1. NM_001273564.1.
    NP_476633.1. NM_057285.4.
    UniGenei Dm.3667.

    3D structure databases

    ProteinModelPortali Q03019.
    SMRi Q03019. Positions 221-387.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 60966. 6 interactions.
    DIPi DIP-23538N.
    IntActi Q03019. 2 interactions.
    MINTi MINT-942016.
    STRINGi 7227.FBpp0080412.

    Proteomic databases

    PaxDbi Q03019.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0080855 ; FBpp0080412 ; FBgn0002673 .
    FBtr0331444 ; FBpp0303861 ; FBgn0002673 .
    FBtr0331445 ; FBpp0303862 ; FBgn0002673 .
    FBtr0331446 ; FBpp0303863 ; FBgn0002673 .
    GeneIDi 34954.
    KEGGi dme:Dmel_CG4965.
    UCSCi CG4965-RA. d. melanogaster.

    Organism-specific databases

    CTDi 34954.
    FlyBasei FBgn0002673. twe.

    Phylogenomic databases

    eggNOGi COG5105.
    GeneTreei ENSGT00390000018747.
    InParanoidi Q03019.
    OMAi RAKTKSW.
    OrthoDBi EOG757D02.
    PhylomeDBi Q03019.

    Enzyme and pathway databases

    Reactomei REACT_180658. G0 and Early G1.
    REACT_208929. Polo-like kinase mediated events.
    REACT_218568. Activation of ATR in response to replication stress.
    REACT_222733. E2F-enabled inhibition of pre-replication complex formation.
    REACT_222970. p53-Independent DNA Damage Response.
    REACT_225636. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_225883. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

    Miscellaneous databases

    GenomeRNAii 34954.
    NextBioi 791075.

    Gene expression databases

    Bgeei Q03019.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view ]
    Pfami PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PRINTSi PR00716. MPIPHPHTASE.
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Twine, a cdc25 homolog that functions in the male and female germline of Drosophila."
      Alphey L.S., Jimenez J., White-Cooper H., Dawson I., Nurse P., Glover D.M.
      Cell 69:977-988(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster: the Adh region."
      Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T., Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G., Harvey D.A., Hong L., Houston K.A., Hoskins R.A., Johnson G., Martin C., Moshrefi A.R.
      , Palazzolo M., Reese M.G., Spradling A.C., Tsang G., Wan K.H., Whitelaw K., Celniker S.E., Rubin G.M.
      Genetics 153:179-219(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    6. "The Drosophila cdc25 homolog twine is required for meiosis."
      Courtot C., Fankhauser C., Simanis V., Lehner C.F.
      Development 116:405-416(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-426.

    Entry informationi

    Entry nameiTWINE_DROME
    AccessioniPrimary (citable) accession number: Q03019
    Secondary accession number(s): Q9V454
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3