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Protein

NF-kappa-B inhibitor cactus

Gene

cact

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the formation of the dorsoventral pattern. It inhibits nuclear translocation of the dorsal morphogen in the dorsal region of the embryo. Acts as a negative regulator of the NF-kappa-B (rel) signaling pathway. Cact is degraded by IKKbeta, this is essential for NF-kappa-B (rel) activation.4 Publications

GO - Molecular functioni

  • transcription factor binding Source: FlyBase

GO - Biological processi

  • antifungal humoral response Source: FlyBase
  • antimicrobial humoral response Source: FlyBase
  • defense response Source: FlyBase
  • dorsal/ventral axis specification Source: UniProtKB
  • dorsal appendage formation Source: FlyBase
  • hemopoiesis Source: FlyBase
  • innate immune response Source: FlyBase
  • nervous system development Source: FlyBase
  • oogenesis Source: FlyBase
  • positive regulation of antifungal peptide biosynthetic process Source: FlyBase
  • response to fungus Source: FlyBase
  • Toll signaling pathway Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein

Enzyme and pathway databases

ReactomeiR-DME-1169091 Activation of NF-kappaB in B cells
R-DME-202424 Downstream TCR signaling
R-DME-209406 Degradation of NF-kappa-B inhibitor, CACT
R-DME-209560 NF-kB is activated and signals survival
R-DME-214842 DL and DIF homodimers bind to TUB and phosphorylated PLL in the TL receptor 'signalling complex'
R-DME-214844 DL and DIF homodimers complexed with CACT are all phosphorylated in the TL receptor 'signalling complex'
R-DME-214869 Phosphorylated CACT, DL and DIF homodimers dissociate from the TL receptor 'signalling complex'
R-DME-2871837 FCERI mediated NF-kB activation
R-DME-5607764 CLEC7A (Dectin-1) signaling
R-DME-9020702 Interleukin-1 signaling
SignaLinkiQ03017

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B inhibitor cactus
Gene namesi
Name:cact
ORF Names:CG5848
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000250 cact

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670551 – 500NF-kappa-B inhibitor cactusAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45Phosphoserine; by PKCSequence analysis1
Modified residuei144Phosphoserine; by PKCSequence analysis1
Modified residuei183Phosphothreonine; by PKCSequence analysis1
Modified residuei293Phosphothreonine; by PKCSequence analysis1
Modified residuei319Phosphothreonine; by PKCSequence analysis1
Modified residuei395Phosphoserine; by PKCSequence analysis1

Post-translational modificationi

Activated IKKbeta phosphorylates cact.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ03017
PRIDEiQ03017

PTM databases

iPTMnetiQ03017

Expressioni

Tissue specificityi

Expressed in ovary (at protein level).

Developmental stagei

Expressed during embryogenesis (at protein level). Isoform A is expressed in ovaries and 0-1 hour embryos. After 2-3 hours unphosphorylated zygotic protein is expressed. Between 4-8 hours phosphorylated zygotic protein is expressed. After 12 hours the amount of unphosphorylated zygotic protein increases until, by the end of embryogenesis, it is the most abundant form of cactus.1 Publication

Gene expression databases

BgeeiFBgn0000250
ExpressionAtlasiQ03017 baseline and differential
GenevisibleiQ03017 DM

Interactioni

Subunit structurei

Phosphorylated isoform A binds to dorsal (dl); inhibits dl translocation to the nucleus and therefore from binding to DNA. In vitro, interacts with IKKbeta. Interacts with cactin and kappa-B-Ras.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi60980, 52 interactors
DIPiDIP-17846N
IntActiQ03017, 12 interactors
MINTiQ03017
STRINGi7227.FBpp0080402

Structurei

3D structure databases

ProteinModelPortaliQ03017
SMRiQ03017
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati229 – 261ANK 1Add BLAST33
Repeati265 – 294ANK 2Add BLAST30
Repeati298 – 327ANK 3Add BLAST30
Repeati361 – 390ANK 4Add BLAST30
Repeati395 – 424ANK 5Add BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi120 – 136Asp/Glu-rich (acidic)Add BLAST17
Compositional biasi469 – 475Asp/Glu-rich (acidic)7

Sequence similaritiesi

Belongs to the NF-kappa-B inhibitor family.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00550000074527
InParanoidiQ03017
KOiK04734
OMAiIPQWTVQ
OrthoDBiEOG091G0GOB

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 5 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 4 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q03017-1) [UniParc]FASTAAdd to basket
Also known as: B, D, Maternal/zygotic

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPSPTKAAEA ATKATATSDC SCSAASVEQR APSNAANPSS SLATSGKIGG
60 70 80 90 100
KTQDQTAAIN KQKEFAVPNE TSDSGFISGP QSSQIFSEEI VPDSEEQDKD
110 120 130 140 150
QQESAPQKEQ PVVLDSGIID EEEDQEEQEK EEEHQDTTTA TADSMRLKHS
160 170 180 190 200
ADTGIPQWTV ESHLVSRGEQ LNNLGQSSST QITGRSKVQS STASTANANP
210 220 230 240 250
SGSGATSSAP PSSINIMNAW EQFYQQNDDG DTPLHLACIS GSVDVVAALI
260 270 280 290 300
RMAPHPCLLN IQNDVAQTPL HLAALTAQPN IMRILLLAGA EPTVRDRHGN
310 320 330 340 350
TALHLSCIAG EKQCVRALTE KFGATEIHEA HRQYGHRSND KAVSSLSYAC
360 370 380 390 400
LPADLEIRNY DGERCVHLAA EAGHIDILRI LVSHGADINA REGKSGRTPL
410 420 430 440 450
HIAIEGCNED LANFLLDECE KLNLETATYA GLTAYQFACI MNKSRMQNIL
460 470 480 490 500
EKRGAETVTP PDSDYDSSDI EDLDDTKMYD RFGDPRYFVS YNGGNPMTVA
Length:500
Mass (Da):53,818
Last modified:November 2, 2001 - v2
Checksum:i9E9711B97CDB499F
GO
Isoform C (identifier: Q03017-2) [UniParc]FASTAAdd to basket
Also known as: Zygotic

The sequence of this isoform differs from the canonical sequence as follows:
     478-500: MYDRFGDPRYFVSYNGGNPMTVA → VSVTA

Show »
Length:482
Mass (Da):51,636
Checksum:i334A444730896055
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti196A → G in AAA85908 (PubMed:1423618).Curated1
Sequence conflicti196A → G in AAA28407 (PubMed:1423619).Curated1
Sequence conflicti222Q → R in AAA28407 (PubMed:1423619).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000286478 – 500MYDRF…PMTVA → VSVTA in isoform C. CuratedAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04964 mRNA Translation: AAA85908.1
L03367 Genomic DNA Translation: AAA28407.1
L03368 Genomic DNA Translation: AAA28408.1
AE014134 Genomic DNA Translation: AAN10936.1
AE014134 Genomic DNA Translation: AAN10937.1
AE014134 Genomic DNA Translation: AAS64714.1
AY069399 mRNA Translation: AAL39544.1
PIRiA44269
B44268
RefSeqiNP_001260496.1, NM_001273567.1 [Q03017-1]
NP_476942.1, NM_057594.4 [Q03017-1]
NP_476943.1, NM_057595.4 [Q03017-2]
NP_723960.1, NM_165152.2 [Q03017-1]
NP_995721.1, NM_205999.2 [Q03017-1]
UniGeneiDm.2150

Genome annotation databases

EnsemblMetazoaiFBtr0080844; FBpp0080402; FBgn0000250 [Q03017-1]
FBtr0080845; FBpp0080403; FBgn0000250 [Q03017-1]
FBtr0080846; FBpp0080404; FBgn0000250 [Q03017-2]
FBtr0080847; FBpp0089296; FBgn0000250 [Q03017-1]
FBtr0337081; FBpp0308004; FBgn0000250 [Q03017-1]
GeneIDi34969
KEGGidme:Dmel_CG5848
UCSCiCG5848-RA d. melanogaster [Q03017-1]
CG5848-RD d. melanogaster

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCACT_DROME
AccessioniPrimary (citable) accession number: Q03017
Secondary accession number(s): A4V0S3, Q0E8Q3, Q9V3M6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 2, 2001
Last modified: May 23, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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