Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hematopoietically-expressed homeobox protein HHEX

Gene

HHEX

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi137 – 196HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • chromatin binding Source: Ensembl
  • DNA binding, bending Source: BHF-UCL
  • eukaryotic initiation factor 4E binding Source: BHF-UCL
  • HMG box domain binding Source: Ensembl
  • protein homodimerization activity Source: BHF-UCL
  • repressing transcription factor binding Source: BHF-UCL
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: BHF-UCL
  • TBP-class protein binding Source: BHF-UCL
  • transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcription factor binding Source: BHF-UCL
  • transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processDifferentiation, Transcription, Transcription regulation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Hematopoietically-expressed homeobox protein HHEX
Short name:
Homeobox protein HEX
Alternative name(s):
Homeobox protein PRH
Gene namesi
Name:HHEX
Synonyms:HEX, PRH, PRHX
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000152804.10
HGNCiHGNC:4901 HHEX
MIMi604420 gene
neXtProtiNX_Q03014

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3087
OpenTargetsiENSG00000152804
PharmGKBiPA29274

Polymorphism and mutation databases

BioMutaiHHEX
DMDMi547658

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000490741 – 270Hematopoietically-expressed homeobox protein HHEXAdd BLAST270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ03014
MaxQBiQ03014
PaxDbiQ03014
PeptideAtlasiQ03014
PRIDEiQ03014

PTM databases

iPTMnetiQ03014
PhosphoSitePlusiQ03014

Expressioni

Tissue specificityi

Liver and promyelocytic leukemia cell line HL-60.3 Publications

Developmental stagei

Expressed during hematopoiesis.

Gene expression databases

BgeeiENSG00000152804
CleanExiHS_HHEX
ExpressionAtlasiQ03014 baseline and differential
GenevisibleiQ03014 HS

Organism-specific databases

HPAiHPA051894
HPA055460

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • eukaryotic initiation factor 4E binding Source: BHF-UCL
  • HMG box domain binding Source: Ensembl
  • protein homodimerization activity Source: BHF-UCL
  • repressing transcription factor binding Source: BHF-UCL
  • TBP-class protein binding Source: BHF-UCL
  • transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi109335, 10 interactors
IntActiQ03014, 25 interactors
STRINGi9606.ENSP00000282728

Structurei

Secondary structure

1270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi146 – 158Combined sources13
Helixi164 – 173Combined sources10
Helixi178 – 194Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E1ONMR-A138-194[»]
ProteinModelPortaliQ03014
SMRiQ03014
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03014

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 133Pro-richAdd BLAST133

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0483 Eukaryota
ENOG410Z2UE LUCA
GeneTreeiENSGT00910000143996
HOGENOMiHOG000049110
HOVERGENiHBG055460
InParanoidiQ03014
KOiK08024
OMAiDNSCDQR
OrthoDBiEOG091G0RNV
PhylomeDBiQ03014
TreeFamiTF325047

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR020479 Homeobox_metazoa
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PRINTSiPR00024 HOMEOBOX
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q03014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYPHPGPAA GAVGVPLYAP TPLLQPAHPT PFYIEDILGR GPAAPTPAPT
60 70 80 90 100
LPSPNSSFTS LVSPYRTPVY EPTPIHPAFS HHSAAALAAA YGPGGFGGPL
110 120 130 140 150
YPFPRTVNDY THALLRHDPL GKPLLWSPFL QRPLHKRKGG QVRFSNDQTI
160 170 180 190 200
ELEKKFETQK YLSPPERKRL AKMLQLSERQ VKTWFQNRRA KWRRLKQENP
210 220 230 240 250
QSNKKEELES LDSSCDQRQD LPSEQNKGAS LDSSQCSPSP ASQEDLESEI
260 270
SEDSDQEVDI EGDKSYFNAG
Length:270
Mass (Da):30,022
Last modified:June 1, 1994 - v1
Checksum:i9C16BB6D494475FC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115L → V in AAA02988 (PubMed:8103988).Curated1
Sequence conflicti171A → T in AAH14336 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67235 mRNA Translation: CAA47661.1
L16499 mRNA Translation: AAA02988.1
AK314891 mRNA Translation: BAG37405.1
AL590080 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW50087.1
CH471066 Genomic DNA Translation: EAW50088.1
BC014336 mRNA Translation: AAH14336.1
BC015110 mRNA Translation: AAH15110.1
BC050638 mRNA Translation: AAH50638.1
Z21533 mRNA Translation: CAA79730.1
CCDSiCCDS7423.1
PIRiJN0767
RefSeqiNP_002720.1, NM_002729.4
UniGeneiHs.118651

Genome annotation databases

EnsembliENST00000282728; ENSP00000282728; ENSG00000152804
GeneIDi3087
KEGGihsa:3087
UCSCiuc001kid.4 human

Similar proteinsi

Entry informationi

Entry nameiHHEX_HUMAN
AccessioniPrimary (citable) accession number: Q03014
Secondary accession number(s): B1AQ17, Q96CE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health