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Protein

Hematopoietically-expressed homeobox protein HHEX

Gene

HHEX

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi137 – 19660HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • chromatin binding Source: Ensembl
  • DNA binding, bending Source: BHF-UCL
  • eukaryotic initiation factor 4E binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • repressing transcription factor binding Source: BHF-UCL
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: BHF-UCL
  • TBP-class protein binding Source: BHF-UCL
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor binding Source: BHF-UCL
  • transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation, Wnt signaling pathway

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hematopoietically-expressed homeobox protein HHEX
Short name:
Homeobox protein HEX
Alternative name(s):
Homeobox protein PRH
Gene namesi
Name:HHEX
Synonyms:HEX, PRH, PRHX
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:4901. HHEX.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • nucleus Source: BHF-UCL
  • protein-DNA complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29274.

Polymorphism and mutation databases

BioMutaiHHEX.
DMDMi547658.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Hematopoietically-expressed homeobox protein HHEXPRO_0000049074Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ03014.
MaxQBiQ03014.
PaxDbiQ03014.
PeptideAtlasiQ03014.
PRIDEiQ03014.

PTM databases

iPTMnetiQ03014.
PhosphoSiteiQ03014.

Expressioni

Tissue specificityi

Liver and promyelocytic leukemia cell line HL-60.3 Publications

Developmental stagei

Expressed during hematopoiesis.

Gene expression databases

BgeeiENSG00000152804.
CleanExiHS_HHEX.
ExpressionAtlasiQ03014. baseline and differential.
GenevisibleiQ03014. HS.

Organism-specific databases

HPAiHPA051894.
HPA055460.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KRTAP10-5P603703EBI-747421,EBI-10172150
KRTAP10-7P604093EBI-747421,EBI-10172290
KRTAP10-8P604103EBI-747421,EBI-10171774

GO - Molecular functioni

  • eukaryotic initiation factor 4E binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • repressing transcription factor binding Source: BHF-UCL
  • TBP-class protein binding Source: BHF-UCL
  • transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi109335. 10 interactions.
IntActiQ03014. 7 interactions.
MINTiMINT-1439118.
STRINGi9606.ENSP00000282728.

Structurei

Secondary structure

1
270
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi146 – 15813Combined sources
Helixi164 – 17310Combined sources
Helixi178 – 19417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E1ONMR-A138-194[»]
ProteinModelPortaliQ03014.
SMRiQ03014. Positions 141-194.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03014.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 133133Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0483. Eukaryota.
ENOG410Z2UE. LUCA.
GeneTreeiENSGT00840000129678.
HOGENOMiHOG000049110.
HOVERGENiHBG055460.
InParanoidiQ03014.
KOiK08024.
OMAiAYGPSGF.
OrthoDBiEOG091G0RNV.
PhylomeDBiQ03014.
TreeFamiTF325047.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYPHPGPAA GAVGVPLYAP TPLLQPAHPT PFYIEDILGR GPAAPTPAPT
60 70 80 90 100
LPSPNSSFTS LVSPYRTPVY EPTPIHPAFS HHSAAALAAA YGPGGFGGPL
110 120 130 140 150
YPFPRTVNDY THALLRHDPL GKPLLWSPFL QRPLHKRKGG QVRFSNDQTI
160 170 180 190 200
ELEKKFETQK YLSPPERKRL AKMLQLSERQ VKTWFQNRRA KWRRLKQENP
210 220 230 240 250
QSNKKEELES LDSSCDQRQD LPSEQNKGAS LDSSQCSPSP ASQEDLESEI
260 270
SEDSDQEVDI EGDKSYFNAG
Length:270
Mass (Da):30,022
Last modified:June 1, 1994 - v1
Checksum:i9C16BB6D494475FC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151L → V in AAA02988 (PubMed:8103988).Curated
Sequence conflicti171 – 1711A → T in AAH14336 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67235 mRNA. Translation: CAA47661.1.
L16499 mRNA. Translation: AAA02988.1.
AK314891 mRNA. Translation: BAG37405.1.
AL590080 Genomic DNA. Translation: CAH74086.1.
CH471066 Genomic DNA. Translation: EAW50087.1.
CH471066 Genomic DNA. Translation: EAW50088.1.
BC014336 mRNA. Translation: AAH14336.1.
BC015110 mRNA. Translation: AAH15110.1.
BC050638 mRNA. Translation: AAH50638.1.
Z21533 mRNA. Translation: CAA79730.1.
CCDSiCCDS7423.1.
PIRiJN0767.
RefSeqiNP_002720.1. NM_002729.4.
UniGeneiHs.118651.

Genome annotation databases

EnsembliENST00000282728; ENSP00000282728; ENSG00000152804.
GeneIDi3087.
KEGGihsa:3087.
UCSCiuc001kid.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67235 mRNA. Translation: CAA47661.1.
L16499 mRNA. Translation: AAA02988.1.
AK314891 mRNA. Translation: BAG37405.1.
AL590080 Genomic DNA. Translation: CAH74086.1.
CH471066 Genomic DNA. Translation: EAW50087.1.
CH471066 Genomic DNA. Translation: EAW50088.1.
BC014336 mRNA. Translation: AAH14336.1.
BC015110 mRNA. Translation: AAH15110.1.
BC050638 mRNA. Translation: AAH50638.1.
Z21533 mRNA. Translation: CAA79730.1.
CCDSiCCDS7423.1.
PIRiJN0767.
RefSeqiNP_002720.1. NM_002729.4.
UniGeneiHs.118651.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E1ONMR-A138-194[»]
ProteinModelPortaliQ03014.
SMRiQ03014. Positions 141-194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109335. 10 interactions.
IntActiQ03014. 7 interactions.
MINTiMINT-1439118.
STRINGi9606.ENSP00000282728.

PTM databases

iPTMnetiQ03014.
PhosphoSiteiQ03014.

Polymorphism and mutation databases

BioMutaiHHEX.
DMDMi547658.

Proteomic databases

EPDiQ03014.
MaxQBiQ03014.
PaxDbiQ03014.
PeptideAtlasiQ03014.
PRIDEiQ03014.

Protocols and materials databases

DNASUi3087.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000282728; ENSP00000282728; ENSG00000152804.
GeneIDi3087.
KEGGihsa:3087.
UCSCiuc001kid.4. human.

Organism-specific databases

CTDi3087.
GeneCardsiHHEX.
HGNCiHGNC:4901. HHEX.
HPAiHPA051894.
HPA055460.
MIMi604420. gene.
neXtProtiNX_Q03014.
PharmGKBiPA29274.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0483. Eukaryota.
ENOG410Z2UE. LUCA.
GeneTreeiENSGT00840000129678.
HOGENOMiHOG000049110.
HOVERGENiHBG055460.
InParanoidiQ03014.
KOiK08024.
OMAiAYGPSGF.
OrthoDBiEOG091G0RNV.
PhylomeDBiQ03014.
TreeFamiTF325047.

Miscellaneous databases

EvolutionaryTraceiQ03014.
GeneWikiiHHEX.
GenomeRNAii3087.
PROiQ03014.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000152804.
CleanExiHS_HHEX.
ExpressionAtlasiQ03014. baseline and differential.
GenevisibleiQ03014. HS.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHHEX_HUMAN
AccessioniPrimary (citable) accession number: Q03014
Secondary accession number(s): B1AQ17, Q96CE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.