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Q03014

- HHEX_HUMAN

UniProt

Q03014 - HHEX_HUMAN

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Protein

Hematopoietically-expressed homeobox protein HHEX

Gene

HHEX

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi137 – 19660HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: Ensembl
  2. DNA binding, bending Source: BHF-UCL
  3. eukaryotic initiation factor 4E binding Source: BHF-UCL
  4. protein homodimerization activity Source: BHF-UCL
  5. repressing transcription factor binding Source: BHF-UCL
  6. sequence-specific DNA binding Source: BHF-UCL
  7. TBP-class protein binding Source: BHF-UCL
  8. transcription factor binding Source: BHF-UCL
  9. transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

  1. anterior/posterior pattern specification Source: UniProtKB
  2. B cell differentiation Source: BHF-UCL
  3. cell differentiation Source: BHF-UCL
  4. cell proliferation Source: BHF-UCL
  5. DNA conformation change Source: BHF-UCL
  6. embryonic heart tube development Source: Ensembl
  7. endoderm development Source: Ensembl
  8. forebrain morphogenesis Source: Ensembl
  9. gall bladder development Source: Ensembl
  10. hepatic duct development Source: Ensembl
  11. hepatoblast differentiation Source: Ensembl
  12. hepatocyte differentiation Source: Ensembl
  13. interkinetic nuclear migration Source: Ensembl
  14. in utero embryonic development Source: Ensembl
  15. mRNA export from nucleus Source: BHF-UCL
  16. multicellular organism growth Source: Ensembl
  17. myeloid leukocyte differentiation Source: Ensembl
  18. negative regulation of angiogenesis Source: BHF-UCL
  19. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
  20. negative regulation of transcription, DNA-templated Source: BHF-UCL
  21. negative regulation of transcription by competitive promoter binding Source: BHF-UCL
  22. negative regulation of transcription by transcription factor localization Source: BHF-UCL
  23. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  24. negative regulation of vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
  25. pancreas development Source: Ensembl
  26. poly(A)+ mRNA export from nucleus Source: BHF-UCL
  27. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  28. positive regulation of Wnt signaling pathway Source: UniProtKB
  29. primary lung bud formation Source: Ensembl
  30. primitive streak formation Source: Ensembl
  31. protein localization to nucleus Source: BHF-UCL
  32. regulation of cell proliferation Source: Ensembl
  33. response to peptide hormone Source: Ensembl
  34. response to wounding Source: Ensembl
  35. thyroid gland development Source: Ensembl
  36. transcription, DNA-templated Source: UniProtKB-KW
  37. vasculogenesis Source: Ensembl
  38. Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation, Wnt signaling pathway

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hematopoietically-expressed homeobox protein HHEX
Short name:
Homeobox protein HEX
Alternative name(s):
Homeobox protein PRH
Gene namesi
Name:HHEX
Synonyms:HEX, PRH, PRHX
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 10

Organism-specific databases

HGNCiHGNC:4901. HHEX.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. cytoplasm Source: BHF-UCL
  2. nucleus Source: BHF-UCL
  3. protein-DNA complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29274.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Hematopoietically-expressed homeobox protein HHEXPRO_0000049074Add
BLAST

Proteomic databases

MaxQBiQ03014.
PaxDbiQ03014.
PRIDEiQ03014.

PTM databases

PhosphoSiteiQ03014.

Expressioni

Tissue specificityi

Liver and promyelocytic leukemia cell line HL-60.3 Publications

Developmental stagei

Expressed during hematopoiesis.

Gene expression databases

BgeeiQ03014.
CleanExiHS_HHEX.
ExpressionAtlasiQ03014. baseline and differential.
GenevestigatoriQ03014.

Organism-specific databases

HPAiHPA051894.

Interactioni

Protein-protein interaction databases

BioGridi109335. 8 interactions.
IntActiQ03014. 4 interactions.
MINTiMINT-1439118.
STRINGi9606.ENSP00000282728.

Structurei

Secondary structure

1
270
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi146 – 15813
Helixi164 – 17310
Helixi178 – 19417

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E1ONMR-A138-194[»]
ProteinModelPortaliQ03014.
SMRiQ03014. Positions 141-194.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03014.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 133133Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG252905.
GeneTreeiENSGT00730000110534.
HOGENOMiHOG000049110.
HOVERGENiHBG055460.
InParanoidiQ03014.
KOiK08024.
OMAiDNSCDQR.
OrthoDBiEOG7M0NS8.
PhylomeDBiQ03014.
TreeFamiTF325047.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03014 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQYPHPGPAA GAVGVPLYAP TPLLQPAHPT PFYIEDILGR GPAAPTPAPT
60 70 80 90 100
LPSPNSSFTS LVSPYRTPVY EPTPIHPAFS HHSAAALAAA YGPGGFGGPL
110 120 130 140 150
YPFPRTVNDY THALLRHDPL GKPLLWSPFL QRPLHKRKGG QVRFSNDQTI
160 170 180 190 200
ELEKKFETQK YLSPPERKRL AKMLQLSERQ VKTWFQNRRA KWRRLKQENP
210 220 230 240 250
QSNKKEELES LDSSCDQRQD LPSEQNKGAS LDSSQCSPSP ASQEDLESEI
260 270
SEDSDQEVDI EGDKSYFNAG
Length:270
Mass (Da):30,022
Last modified:June 1, 1994 - v1
Checksum:i9C16BB6D494475FC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151L → V in AAA02988. (PubMed:8103988)Curated
Sequence conflicti171 – 1711A → T in AAH14336. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67235 mRNA. Translation: CAA47661.1.
L16499 mRNA. Translation: AAA02988.1.
AK314891 mRNA. Translation: BAG37405.1.
AL590080 Genomic DNA. Translation: CAH74086.1.
CH471066 Genomic DNA. Translation: EAW50087.1.
CH471066 Genomic DNA. Translation: EAW50088.1.
BC014336 mRNA. Translation: AAH14336.1.
BC015110 mRNA. Translation: AAH15110.1.
BC050638 mRNA. Translation: AAH50638.1.
Z21533 mRNA. Translation: CAA79730.1.
CCDSiCCDS7423.1.
PIRiJN0767.
RefSeqiNP_002720.1. NM_002729.4.
UniGeneiHs.118651.

Genome annotation databases

EnsembliENST00000282728; ENSP00000282728; ENSG00000152804.
GeneIDi3087.
KEGGihsa:3087.
UCSCiuc001kid.3. human.

Polymorphism databases

DMDMi547658.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67235 mRNA. Translation: CAA47661.1 .
L16499 mRNA. Translation: AAA02988.1 .
AK314891 mRNA. Translation: BAG37405.1 .
AL590080 Genomic DNA. Translation: CAH74086.1 .
CH471066 Genomic DNA. Translation: EAW50087.1 .
CH471066 Genomic DNA. Translation: EAW50088.1 .
BC014336 mRNA. Translation: AAH14336.1 .
BC015110 mRNA. Translation: AAH15110.1 .
BC050638 mRNA. Translation: AAH50638.1 .
Z21533 mRNA. Translation: CAA79730.1 .
CCDSi CCDS7423.1.
PIRi JN0767.
RefSeqi NP_002720.1. NM_002729.4.
UniGenei Hs.118651.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2E1O NMR - A 138-194 [» ]
ProteinModelPortali Q03014.
SMRi Q03014. Positions 141-194.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 109335. 8 interactions.
IntActi Q03014. 4 interactions.
MINTi MINT-1439118.
STRINGi 9606.ENSP00000282728.

PTM databases

PhosphoSitei Q03014.

Polymorphism databases

DMDMi 547658.

Proteomic databases

MaxQBi Q03014.
PaxDbi Q03014.
PRIDEi Q03014.

Protocols and materials databases

DNASUi 3087.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000282728 ; ENSP00000282728 ; ENSG00000152804 .
GeneIDi 3087.
KEGGi hsa:3087.
UCSCi uc001kid.3. human.

Organism-specific databases

CTDi 3087.
GeneCardsi GC10P094439.
HGNCi HGNC:4901. HHEX.
HPAi HPA051894.
MIMi 604420. gene.
neXtProti NX_Q03014.
PharmGKBi PA29274.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG252905.
GeneTreei ENSGT00730000110534.
HOGENOMi HOG000049110.
HOVERGENi HBG055460.
InParanoidi Q03014.
KOi K08024.
OMAi DNSCDQR.
OrthoDBi EOG7M0NS8.
PhylomeDBi Q03014.
TreeFami TF325047.

Miscellaneous databases

EvolutionaryTracei Q03014.
GeneWikii HHEX.
GenomeRNAii 3087.
NextBioi 12253.
PROi Q03014.
SOURCEi Search...

Gene expression databases

Bgeei Q03014.
CleanExi HS_HHEX.
ExpressionAtlasi Q03014. baseline and differential.
Genevestigatori Q03014.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
[Graphical view ]
PRINTSi PR00024. HOMEOBOX.
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel vertebrate homeobox gene expressed in haematopoietic cells."
    Crompton M.R., Bartlett T.J., Macgregor A.D., Manfioletti G., Buratti E., Giancotti V., Goodwin G.H.
    Nucleic Acids Res. 20:5661-5667(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "PCR cloning of an orphan homeobox gene (PRH) preferentially expressed in myeloid and liver cells."
    Hromas R.A., Collins S.J., Radich J.
    Biochem. Biophys. Res. Commun. 195:976-983(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Myeloid leukemia cell.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  4. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow and Uterus.
  7. "HEX: a novel homeobox gene expressed during haematopoiesis and conserved between mouse and human."
    Bedford F.K., Ashworth A., Enver T., Wiedemann L.M.
    Nucleic Acids Res. 21:1245-1249(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-270, TISSUE SPECIFICITY.
    Tissue: Leukemia and Peripheral blood monocyte.
  8. "A homology-based molecular model of the proline-rich homeodomain protein Prh, from haematopoietic cells."
    Neidle S., Goodwin G.H.
    FEBS Lett. 345:93-98(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 136-196.
  9. "Solution structure of RSGI RUH-028, a homeobox domain from human."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 138-194.

Entry informationi

Entry nameiHHEX_HUMAN
AccessioniPrimary (citable) accession number: Q03014
Secondary accession number(s): B1AQ17, Q96CE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3