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Q03014

- HHEX_HUMAN

UniProt

Q03014 - HHEX_HUMAN

Protein

Hematopoietically-expressed homeobox protein HHEX

Gene

HHEX

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi137 – 19660HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: Ensembl
    2. DNA binding, bending Source: BHF-UCL
    3. eukaryotic initiation factor 4E binding Source: BHF-UCL
    4. protein binding Source: UniProtKB
    5. protein homodimerization activity Source: BHF-UCL
    6. repressing transcription factor binding Source: BHF-UCL
    7. sequence-specific DNA binding Source: BHF-UCL
    8. sequence-specific DNA binding transcription factor activity Source: InterPro
    9. TBP-class protein binding Source: BHF-UCL
    10. transcription factor binding Source: BHF-UCL
    11. transcription regulatory region DNA binding Source: BHF-UCL

    GO - Biological processi

    1. anterior/posterior pattern specification Source: UniProtKB
    2. B cell differentiation Source: BHF-UCL
    3. cell differentiation Source: BHF-UCL
    4. cell proliferation Source: BHF-UCL
    5. common bile duct development Source: Ensembl
    6. DNA conformation change Source: BHF-UCL
    7. embryonic heart tube development Source: Ensembl
    8. endoderm development Source: Ensembl
    9. forebrain morphogenesis Source: Ensembl
    10. gall bladder development Source: Ensembl
    11. hepatic duct development Source: Ensembl
    12. hepatoblast differentiation Source: Ensembl
    13. hepatocyte differentiation Source: Ensembl
    14. interkinetic nuclear migration Source: Ensembl
    15. in utero embryonic development Source: Ensembl
    16. mRNA export from nucleus Source: BHF-UCL
    17. multicellular organism growth Source: Ensembl
    18. myeloid leukocyte differentiation Source: Ensembl
    19. negative regulation of angiogenesis Source: BHF-UCL
    20. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
    21. negative regulation of transcription, DNA-templated Source: BHF-UCL
    22. negative regulation of transcription by competitive promoter binding Source: BHF-UCL
    23. negative regulation of transcription by transcription factor localization Source: BHF-UCL
    24. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    25. negative regulation of vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
    26. pancreas development Source: Ensembl
    27. poly(A)+ mRNA export from nucleus Source: BHF-UCL
    28. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    29. positive regulation of Wnt signaling pathway Source: UniProtKB
    30. primary lung bud formation Source: Ensembl
    31. primitive streak formation Source: Ensembl
    32. protein localization to nucleus Source: BHF-UCL
    33. regulation of cell proliferation Source: Ensembl
    34. response to peptide hormone Source: Ensembl
    35. response to wounding Source: Ensembl
    36. thyroid gland development Source: Ensembl
    37. transcription, DNA-templated Source: UniProtKB-KW
    38. vasculogenesis Source: Ensembl
    39. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Differentiation, Transcription, Transcription regulation, Wnt signaling pathway

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hematopoietically-expressed homeobox protein HHEX
    Short name:
    Homeobox protein HEX
    Alternative name(s):
    Homeobox protein PRH
    Gene namesi
    Name:HHEX
    Synonyms:HEX, PRH, PRHX
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:4901. HHEX.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. cytoplasm Source: BHF-UCL
    2. nucleus Source: BHF-UCL
    3. protein-DNA complex Source: BHF-UCL

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29274.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 270270Hematopoietically-expressed homeobox protein HHEXPRO_0000049074Add
    BLAST

    Proteomic databases

    MaxQBiQ03014.
    PaxDbiQ03014.
    PRIDEiQ03014.

    PTM databases

    PhosphoSiteiQ03014.

    Expressioni

    Tissue specificityi

    Liver and promyelocytic leukemia cell line HL-60.3 Publications

    Developmental stagei

    Expressed during hematopoiesis.

    Gene expression databases

    ArrayExpressiQ03014.
    BgeeiQ03014.
    CleanExiHS_HHEX.
    GenevestigatoriQ03014.

    Organism-specific databases

    HPAiHPA051894.

    Interactioni

    Protein-protein interaction databases

    BioGridi109335. 8 interactions.
    IntActiQ03014. 4 interactions.
    MINTiMINT-1439118.
    STRINGi9606.ENSP00000282728.

    Structurei

    Secondary structure

    1
    270
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi146 – 15813
    Helixi164 – 17310
    Helixi178 – 19417

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2E1ONMR-A138-194[»]
    ProteinModelPortaliQ03014.
    SMRiQ03014. Positions 141-194.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ03014.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1 – 133133Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG252905.
    HOGENOMiHOG000049110.
    HOVERGENiHBG055460.
    InParanoidiQ03014.
    KOiK08024.
    OMAiDNSCDQR.
    OrthoDBiEOG7M0NS8.
    PhylomeDBiQ03014.
    TreeFamiTF325047.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    [Graphical view]
    PRINTSiPR00024. HOMEOBOX.
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03014-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQYPHPGPAA GAVGVPLYAP TPLLQPAHPT PFYIEDILGR GPAAPTPAPT    50
    LPSPNSSFTS LVSPYRTPVY EPTPIHPAFS HHSAAALAAA YGPGGFGGPL 100
    YPFPRTVNDY THALLRHDPL GKPLLWSPFL QRPLHKRKGG QVRFSNDQTI 150
    ELEKKFETQK YLSPPERKRL AKMLQLSERQ VKTWFQNRRA KWRRLKQENP 200
    QSNKKEELES LDSSCDQRQD LPSEQNKGAS LDSSQCSPSP ASQEDLESEI 250
    SEDSDQEVDI EGDKSYFNAG 270
    Length:270
    Mass (Da):30,022
    Last modified:June 1, 1994 - v1
    Checksum:i9C16BB6D494475FC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti115 – 1151L → V in AAA02988. (PubMed:8103988)Curated
    Sequence conflicti171 – 1711A → T in AAH14336. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67235 mRNA. Translation: CAA47661.1.
    L16499 mRNA. Translation: AAA02988.1.
    AK314891 mRNA. Translation: BAG37405.1.
    AL590080 Genomic DNA. Translation: CAH74086.1.
    CH471066 Genomic DNA. Translation: EAW50087.1.
    CH471066 Genomic DNA. Translation: EAW50088.1.
    BC014336 mRNA. Translation: AAH14336.1.
    BC015110 mRNA. Translation: AAH15110.1.
    BC050638 mRNA. Translation: AAH50638.1.
    Z21533 mRNA. Translation: CAA79730.1.
    CCDSiCCDS7423.1.
    PIRiJN0767.
    RefSeqiNP_002720.1. NM_002729.4.
    UniGeneiHs.118651.

    Genome annotation databases

    EnsembliENST00000282728; ENSP00000282728; ENSG00000152804.
    GeneIDi3087.
    KEGGihsa:3087.
    UCSCiuc001kid.3. human.

    Polymorphism databases

    DMDMi547658.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67235 mRNA. Translation: CAA47661.1 .
    L16499 mRNA. Translation: AAA02988.1 .
    AK314891 mRNA. Translation: BAG37405.1 .
    AL590080 Genomic DNA. Translation: CAH74086.1 .
    CH471066 Genomic DNA. Translation: EAW50087.1 .
    CH471066 Genomic DNA. Translation: EAW50088.1 .
    BC014336 mRNA. Translation: AAH14336.1 .
    BC015110 mRNA. Translation: AAH15110.1 .
    BC050638 mRNA. Translation: AAH50638.1 .
    Z21533 mRNA. Translation: CAA79730.1 .
    CCDSi CCDS7423.1.
    PIRi JN0767.
    RefSeqi NP_002720.1. NM_002729.4.
    UniGenei Hs.118651.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2E1O NMR - A 138-194 [» ]
    ProteinModelPortali Q03014.
    SMRi Q03014. Positions 141-194.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109335. 8 interactions.
    IntActi Q03014. 4 interactions.
    MINTi MINT-1439118.
    STRINGi 9606.ENSP00000282728.

    PTM databases

    PhosphoSitei Q03014.

    Polymorphism databases

    DMDMi 547658.

    Proteomic databases

    MaxQBi Q03014.
    PaxDbi Q03014.
    PRIDEi Q03014.

    Protocols and materials databases

    DNASUi 3087.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000282728 ; ENSP00000282728 ; ENSG00000152804 .
    GeneIDi 3087.
    KEGGi hsa:3087.
    UCSCi uc001kid.3. human.

    Organism-specific databases

    CTDi 3087.
    GeneCardsi GC10P094439.
    HGNCi HGNC:4901. HHEX.
    HPAi HPA051894.
    MIMi 604420. gene.
    neXtProti NX_Q03014.
    PharmGKBi PA29274.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG252905.
    HOGENOMi HOG000049110.
    HOVERGENi HBG055460.
    InParanoidi Q03014.
    KOi K08024.
    OMAi DNSCDQR.
    OrthoDBi EOG7M0NS8.
    PhylomeDBi Q03014.
    TreeFami TF325047.

    Miscellaneous databases

    EvolutionaryTracei Q03014.
    GeneWikii HHEX.
    GenomeRNAii 3087.
    NextBioi 12253.
    PROi Q03014.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q03014.
    Bgeei Q03014.
    CleanExi HS_HHEX.
    Genevestigatori Q03014.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    [Graphical view ]
    PRINTSi PR00024. HOMEOBOX.
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel vertebrate homeobox gene expressed in haematopoietic cells."
      Crompton M.R., Bartlett T.J., Macgregor A.D., Manfioletti G., Buratti E., Giancotti V., Goodwin G.H.
      Nucleic Acids Res. 20:5661-5667(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. "PCR cloning of an orphan homeobox gene (PRH) preferentially expressed in myeloid and liver cells."
      Hromas R.A., Collins S.J., Radich J.
      Biochem. Biophys. Res. Commun. 195:976-983(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Myeloid leukemia cell.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.
    4. "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
      , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
      Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone marrow and Uterus.
    7. "HEX: a novel homeobox gene expressed during haematopoiesis and conserved between mouse and human."
      Bedford F.K., Ashworth A., Enver T., Wiedemann L.M.
      Nucleic Acids Res. 21:1245-1249(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-270, TISSUE SPECIFICITY.
      Tissue: Leukemia and Peripheral blood monocyte.
    8. "A homology-based molecular model of the proline-rich homeodomain protein Prh, from haematopoietic cells."
      Neidle S., Goodwin G.H.
      FEBS Lett. 345:93-98(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 136-196.
    9. "Solution structure of RSGI RUH-028, a homeobox domain from human."
      RIKEN structural genomics initiative (RSGI)
      Submitted (NOV-2006) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 138-194.

    Entry informationi

    Entry nameiHHEX_HUMAN
    AccessioniPrimary (citable) accession number: Q03014
    Secondary accession number(s): B1AQ17, Q96CE9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 145 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3