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Protein

Hematopoietically-expressed homeobox protein HHEX

Gene

HHEX

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi137 – 196HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • chromatin binding Source: Ensembl
  • DNA binding, bending Source: BHF-UCL
  • eukaryotic initiation factor 4E binding Source: BHF-UCL
  • HMG box domain binding Source: Ensembl
  • protein homodimerization activity Source: BHF-UCL
  • repressing transcription factor binding Source: BHF-UCL
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: BHF-UCL
  • TBP-class protein binding Source: BHF-UCL
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor binding Source: BHF-UCL
  • transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processDifferentiation, Transcription, Transcription regulation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Hematopoietically-expressed homeobox protein HHEX
Short name:
Homeobox protein HEX
Alternative name(s):
Homeobox protein PRH
Gene namesi
Name:HHEX
Synonyms:HEX, PRH, PRHX
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000152804.10.
HGNCiHGNC:4901. HHEX.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3087.
OpenTargetsiENSG00000152804.
PharmGKBiPA29274.

Polymorphism and mutation databases

BioMutaiHHEX.
DMDMi547658.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000490741 – 270Hematopoietically-expressed homeobox protein HHEXAdd BLAST270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ03014.
MaxQBiQ03014.
PaxDbiQ03014.
PeptideAtlasiQ03014.
PRIDEiQ03014.

PTM databases

iPTMnetiQ03014.
PhosphoSitePlusiQ03014.

Expressioni

Tissue specificityi

Liver and promyelocytic leukemia cell line HL-60.3 Publications

Developmental stagei

Expressed during hematopoiesis.

Gene expression databases

BgeeiENSG00000152804.
CleanExiHS_HHEX.
ExpressionAtlasiQ03014. baseline and differential.
GenevisibleiQ03014. HS.

Organism-specific databases

HPAiHPA051894.
HPA055460.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • eukaryotic initiation factor 4E binding Source: BHF-UCL
  • HMG box domain binding Source: Ensembl
  • protein homodimerization activity Source: BHF-UCL
  • repressing transcription factor binding Source: BHF-UCL
  • TBP-class protein binding Source: BHF-UCL
  • transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi109335. 10 interactors.
IntActiQ03014. 25 interactors.
MINTiMINT-1439118.
STRINGi9606.ENSP00000282728.

Structurei

Secondary structure

1270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi146 – 158Combined sources13
Helixi164 – 173Combined sources10
Helixi178 – 194Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E1ONMR-A138-194[»]
ProteinModelPortaliQ03014.
SMRiQ03014.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03014.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 133Pro-richAdd BLAST133

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0483. Eukaryota.
ENOG410Z2UE. LUCA.
GeneTreeiENSGT00890000139333.
HOGENOMiHOG000049110.
HOVERGENiHBG055460.
InParanoidiQ03014.
KOiK08024.
OMAiDNSCDQR.
OrthoDBiEOG091G0RNV.
PhylomeDBiQ03014.
TreeFamiTF325047.

Family and domain databases

CDDicd00086. homeodomain. 1 hit.
InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
PRINTSiPR00024. HOMEOBOX.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYPHPGPAA GAVGVPLYAP TPLLQPAHPT PFYIEDILGR GPAAPTPAPT
60 70 80 90 100
LPSPNSSFTS LVSPYRTPVY EPTPIHPAFS HHSAAALAAA YGPGGFGGPL
110 120 130 140 150
YPFPRTVNDY THALLRHDPL GKPLLWSPFL QRPLHKRKGG QVRFSNDQTI
160 170 180 190 200
ELEKKFETQK YLSPPERKRL AKMLQLSERQ VKTWFQNRRA KWRRLKQENP
210 220 230 240 250
QSNKKEELES LDSSCDQRQD LPSEQNKGAS LDSSQCSPSP ASQEDLESEI
260 270
SEDSDQEVDI EGDKSYFNAG
Length:270
Mass (Da):30,022
Last modified:June 1, 1994 - v1
Checksum:i9C16BB6D494475FC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115L → V in AAA02988 (PubMed:8103988).Curated1
Sequence conflicti171A → T in AAH14336 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67235 mRNA. Translation: CAA47661.1.
L16499 mRNA. Translation: AAA02988.1.
AK314891 mRNA. Translation: BAG37405.1.
AL590080 Genomic DNA. Translation: CAH74086.1.
CH471066 Genomic DNA. Translation: EAW50087.1.
CH471066 Genomic DNA. Translation: EAW50088.1.
BC014336 mRNA. Translation: AAH14336.1.
BC015110 mRNA. Translation: AAH15110.1.
BC050638 mRNA. Translation: AAH50638.1.
Z21533 mRNA. Translation: CAA79730.1.
CCDSiCCDS7423.1.
PIRiJN0767.
RefSeqiNP_002720.1. NM_002729.4.
UniGeneiHs.118651.

Genome annotation databases

EnsembliENST00000282728; ENSP00000282728; ENSG00000152804.
GeneIDi3087.
KEGGihsa:3087.
UCSCiuc001kid.4. human.

Similar proteinsi

Entry informationi

Entry nameiHHEX_HUMAN
AccessioniPrimary (citable) accession number: Q03014
Secondary accession number(s): B1AQ17, Q96CE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 27, 2017
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references