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Protein

Hematopoietically-expressed homeobox protein HHEX

Gene

HHEX

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi137 – 19660HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • chromatin binding Source: Ensembl
  • DNA binding, bending Source: BHF-UCL
  • eukaryotic initiation factor 4E binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • repressing transcription factor binding Source: BHF-UCL
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
  • sequence-specific DNA binding Source: BHF-UCL
  • TBP-class protein binding Source: BHF-UCL
  • transcription factor binding Source: BHF-UCL
  • transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation, Wnt signaling pathway

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hematopoietically-expressed homeobox protein HHEX
Short name:
Homeobox protein HEX
Alternative name(s):
Homeobox protein PRH
Gene namesi
Name:HHEX
Synonyms:HEX, PRH, PRHX
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:4901. HHEX.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • nucleus Source: BHF-UCL
  • protein-DNA complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29274.

Polymorphism and mutation databases

BioMutaiHHEX.
DMDMi547658.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Hematopoietically-expressed homeobox protein HHEXPRO_0000049074Add
BLAST

Proteomic databases

MaxQBiQ03014.
PaxDbiQ03014.
PRIDEiQ03014.

PTM databases

PhosphoSiteiQ03014.

Expressioni

Tissue specificityi

Liver and promyelocytic leukemia cell line HL-60.3 Publications

Developmental stagei

Expressed during hematopoiesis.

Gene expression databases

BgeeiQ03014.
CleanExiHS_HHEX.
ExpressionAtlasiQ03014. baseline and differential.
GenevisibleiQ03014. HS.

Organism-specific databases

HPAiHPA051894.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KRTAP10-5P603703EBI-747421,EBI-10172150
KRTAP10-7P604093EBI-747421,EBI-10172290
KRTAP10-8P604103EBI-747421,EBI-10171774

Protein-protein interaction databases

BioGridi109335. 10 interactions.
IntActiQ03014. 7 interactions.
MINTiMINT-1439118.
STRINGi9606.ENSP00000282728.

Structurei

Secondary structure

1
270
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi146 – 15813Combined sources
Helixi164 – 17310Combined sources
Helixi178 – 19417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E1ONMR-A138-194[»]
ProteinModelPortaliQ03014.
SMRiQ03014. Positions 141-194.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03014.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 133133Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG252905.
GeneTreeiENSGT00790000122955.
HOGENOMiHOG000049110.
HOVERGENiHBG055460.
InParanoidiQ03014.
KOiK08024.
OMAiDNSCDQR.
OrthoDBiEOG7M0NS8.
PhylomeDBiQ03014.
TreeFamiTF325047.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYPHPGPAA GAVGVPLYAP TPLLQPAHPT PFYIEDILGR GPAAPTPAPT
60 70 80 90 100
LPSPNSSFTS LVSPYRTPVY EPTPIHPAFS HHSAAALAAA YGPGGFGGPL
110 120 130 140 150
YPFPRTVNDY THALLRHDPL GKPLLWSPFL QRPLHKRKGG QVRFSNDQTI
160 170 180 190 200
ELEKKFETQK YLSPPERKRL AKMLQLSERQ VKTWFQNRRA KWRRLKQENP
210 220 230 240 250
QSNKKEELES LDSSCDQRQD LPSEQNKGAS LDSSQCSPSP ASQEDLESEI
260 270
SEDSDQEVDI EGDKSYFNAG
Length:270
Mass (Da):30,022
Last modified:June 1, 1994 - v1
Checksum:i9C16BB6D494475FC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151L → V in AAA02988 (PubMed:8103988).Curated
Sequence conflicti171 – 1711A → T in AAH14336 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67235 mRNA. Translation: CAA47661.1.
L16499 mRNA. Translation: AAA02988.1.
AK314891 mRNA. Translation: BAG37405.1.
AL590080 Genomic DNA. Translation: CAH74086.1.
CH471066 Genomic DNA. Translation: EAW50087.1.
CH471066 Genomic DNA. Translation: EAW50088.1.
BC014336 mRNA. Translation: AAH14336.1.
BC015110 mRNA. Translation: AAH15110.1.
BC050638 mRNA. Translation: AAH50638.1.
Z21533 mRNA. Translation: CAA79730.1.
CCDSiCCDS7423.1.
PIRiJN0767.
RefSeqiNP_002720.1. NM_002729.4.
UniGeneiHs.118651.

Genome annotation databases

EnsembliENST00000282728; ENSP00000282728; ENSG00000152804.
GeneIDi3087.
KEGGihsa:3087.
UCSCiuc001kid.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67235 mRNA. Translation: CAA47661.1.
L16499 mRNA. Translation: AAA02988.1.
AK314891 mRNA. Translation: BAG37405.1.
AL590080 Genomic DNA. Translation: CAH74086.1.
CH471066 Genomic DNA. Translation: EAW50087.1.
CH471066 Genomic DNA. Translation: EAW50088.1.
BC014336 mRNA. Translation: AAH14336.1.
BC015110 mRNA. Translation: AAH15110.1.
BC050638 mRNA. Translation: AAH50638.1.
Z21533 mRNA. Translation: CAA79730.1.
CCDSiCCDS7423.1.
PIRiJN0767.
RefSeqiNP_002720.1. NM_002729.4.
UniGeneiHs.118651.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E1ONMR-A138-194[»]
ProteinModelPortaliQ03014.
SMRiQ03014. Positions 141-194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109335. 10 interactions.
IntActiQ03014. 7 interactions.
MINTiMINT-1439118.
STRINGi9606.ENSP00000282728.

PTM databases

PhosphoSiteiQ03014.

Polymorphism and mutation databases

BioMutaiHHEX.
DMDMi547658.

Proteomic databases

MaxQBiQ03014.
PaxDbiQ03014.
PRIDEiQ03014.

Protocols and materials databases

DNASUi3087.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000282728; ENSP00000282728; ENSG00000152804.
GeneIDi3087.
KEGGihsa:3087.
UCSCiuc001kid.3. human.

Organism-specific databases

CTDi3087.
GeneCardsiGC10P094439.
HGNCiHGNC:4901. HHEX.
HPAiHPA051894.
MIMi604420. gene.
neXtProtiNX_Q03014.
PharmGKBiPA29274.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG252905.
GeneTreeiENSGT00790000122955.
HOGENOMiHOG000049110.
HOVERGENiHBG055460.
InParanoidiQ03014.
KOiK08024.
OMAiDNSCDQR.
OrthoDBiEOG7M0NS8.
PhylomeDBiQ03014.
TreeFamiTF325047.

Miscellaneous databases

EvolutionaryTraceiQ03014.
GeneWikiiHHEX.
GenomeRNAii3087.
NextBioi12253.
PROiQ03014.
SOURCEiSearch...

Gene expression databases

BgeeiQ03014.
CleanExiHS_HHEX.
ExpressionAtlasiQ03014. baseline and differential.
GenevisibleiQ03014. HS.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel vertebrate homeobox gene expressed in haematopoietic cells."
    Crompton M.R., Bartlett T.J., Macgregor A.D., Manfioletti G., Buratti E., Giancotti V., Goodwin G.H.
    Nucleic Acids Res. 20:5661-5667(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "PCR cloning of an orphan homeobox gene (PRH) preferentially expressed in myeloid and liver cells."
    Hromas R.A., Collins S.J., Radich J.
    Biochem. Biophys. Res. Commun. 195:976-983(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Myeloid leukemia cell.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  4. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow and Uterus.
  7. "HEX: a novel homeobox gene expressed during haematopoiesis and conserved between mouse and human."
    Bedford F.K., Ashworth A., Enver T., Wiedemann L.M.
    Nucleic Acids Res. 21:1245-1249(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-270, TISSUE SPECIFICITY.
    Tissue: Leukemia and Peripheral blood monocyte.
  8. "A homology-based molecular model of the proline-rich homeodomain protein Prh, from haematopoietic cells."
    Neidle S., Goodwin G.H.
    FEBS Lett. 345:93-98(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 136-196.
  9. "Solution structure of RSGI RUH-028, a homeobox domain from human."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 138-194.

Entry informationi

Entry nameiHHEX_HUMAN
AccessioniPrimary (citable) accession number: Q03014
Secondary accession number(s): B1AQ17, Q96CE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 22, 2015
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.