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Protein

Glutathione S-transferase Mu 4

Gene

GSTM4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Active on 1-chloro-2,4-dinitrobenzene.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116SubstrateBy similarity1

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • glutathione binding Source: BHF-UCL
  • glutathione transferase activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL

GO - Biological processi

  • glutathione derivative biosynthetic process Source: Reactome
  • glutathione metabolic process Source: BHF-UCL
  • nitrobenzene metabolic process Source: Ensembl
  • xenobiotic catabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciZFISH:HS09817-MONOMER.
BRENDAi2.5.1.18. 2681.
ReactomeiR-HSA-156590. Glutathione conjugation.
SABIO-RKQ03013.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase Mu 4 (EC:2.5.1.18)
Alternative name(s):
GST class-mu 4
GST-Mu2
GSTM4-4
Gene namesi
Name:GSTM4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:4636. GSTM4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • cytosol Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi2948.
OpenTargetsiENSG00000168765.
PharmGKBiPA29026.

Chemistry databases

ChEMBLiCHEMBL2100.
DrugBankiDB00143. Glutathione.

Polymorphism and mutation databases

BioMutaiGSTM4.
DMDMi1170096.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001858242 – 218Glutathione S-transferase Mu 4Add BLAST217

Proteomic databases

EPDiQ03013.
MaxQBiQ03013.
PaxDbiQ03013.
PeptideAtlasiQ03013.
PRIDEiQ03013.

2D gel databases

REPRODUCTION-2DPAGEIPI00008770.

PTM databases

iPTMnetiQ03013.
PhosphoSitePlusiQ03013.

Expressioni

Tissue specificityi

Expressed in a wide variety of tissues.

Gene expression databases

BgeeiENSG00000168765.
CleanExiHS_GSTM4.
ExpressionAtlasiQ03013. baseline and differential.
GenevisibleiQ03013. HS.

Organism-specific databases

HPAiHPA055972.
HPA055973.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
GSTM3Q6FGJ93EBI-713363,EBI-10209603

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL

Protein-protein interaction databases

BioGridi109203. 14 interactors.
IntActiQ03013. 10 interactors.
MINTiMINT-1368381.
STRINGi9606.ENSP00000358851.

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Helixi14 – 23Combined sources10
Beta strandi28 – 33Combined sources6
Turni38 – 41Combined sources4
Helixi44 – 50Combined sources7
Helixi51 – 53Combined sources3
Beta strandi60 – 64Combined sources5
Beta strandi69 – 72Combined sources4
Helixi73 – 83Combined sources11
Helixi91 – 114Combined sources24
Helixi120 – 142Combined sources23
Beta strandi150 – 152Combined sources3
Helixi155 – 170Combined sources16
Helixi179 – 190Combined sources12
Helixi192 – 198Combined sources7
Turni214 – 216Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GTUX-ray3.30A/B/C/D/E/F/G/H2-218[»]
ProteinModelPortaliQ03013.
SMRiQ03013.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03013.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 88GST N-terminalAdd BLAST87
Domaini90 – 208GST C-terminalAdd BLAST119

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 8Glutathione bindingBy similarity2
Regioni46 – 50Glutathione bindingBy similarity5
Regioni59 – 60Glutathione bindingBy similarity2
Regioni72 – 73Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Mu family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiENOG410IN5J. Eukaryota.
ENOG4110YU0. LUCA.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115735.
HOVERGENiHBG106842.
InParanoidiQ03013.
KOiK00799.
OMAiAQPIRYL.
PhylomeDBiQ03013.
TreeFamiTF353040.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01267. GSTRNSFRASEM.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q03013-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSMTLGYWDI RGLAHAIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK
60 70 80 90 100
FKLGLDFPNL PYLIDGAHKI TQSNAILCYI ARKHNLCGET EEEKIRVDIL
110 120 130 140 150
ENQAMDVSNQ LARVCYSPDF EKLKPEYLEE LPTMMQHFSQ FLGKRPWFVG
160 170 180 190 200
DKITFVDFLA YDVLDLHRIF EPNCLDAFPN LKDFISRFEG LEKISAYMKS
210
SRFLPKPLYT RVAVWGNK
Length:218
Mass (Da):25,561
Last modified:January 23, 2007 - v3
Checksum:i35E96FA54D566B1E
GO
Isoform 2 (identifier: Q03013-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     190-195: GLEKIS → VSCGIM
     196-218: Missing.

Show »
Length:195
Mass (Da):22,829
Checksum:i32F3BEF15BB378D3
GO
Isoform 3 (identifier: Q03013-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-120: Missing.

Show »
Length:157
Mass (Da):18,627
Checksum:i095AFD32871D3B4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 4SMT → PMI in CAA48637 (PubMed:8471052).Curated3
Sequence conflicti17I → M in AAA57346 (PubMed:8349586).Curated1
Sequence conflicti37D → G in CAA48637 (PubMed:8471052).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0339792S → P.Corresponds to variant rs3211190dbSNPEnsembl.1
Natural variantiVAR_033980160A → V.Corresponds to variant rs17838158dbSNPEnsembl.1
Natural variantiVAR_049487208L → V.Corresponds to variant rs112611763dbSNPEnsembl.1
Natural variantiVAR_049488209Y → F.Corresponds to variant rs112330158dbSNPEnsembl.1
Natural variantiVAR_049489211R → K.Corresponds to variant rs200675176dbSNPEnsembl.1
Natural variantiVAR_049490212V → M.1 PublicationCorresponds to variant rs149370166dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04768860 – 120Missing in isoform 3. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_011773190 – 195GLEKIS → VSCGIM in isoform 2. 1 Publication6
Alternative sequenceiVSP_011774196 – 218Missing in isoform 2. 1 PublicationAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96234 mRNA. Translation: AAA57347.1.
M96233 Genomic DNA. Translation: AAA57346.1.
X68677 Genomic DNA. Translation: CAA48637.1.
M99422 mRNA. Translation: AAA58623.1.
DQ062813 mRNA. Translation: AAY98515.1.
CR541869 mRNA. Translation: CAG46667.1.
AK291880 mRNA. Translation: BAF84569.1.
AC000031 Genomic DNA. No translation available.
CH471122 Genomic DNA. Translation: EAW56405.1.
BC015513 mRNA. Translation: AAH15513.1.
BC108729 mRNA. Translation: AAI08730.1.
X56837 Genomic DNA. Translation: CAA40167.1.
CCDSiCCDS806.1. [Q03013-2]
CCDS807.1. [Q03013-1]
PIRiA47486.
S32425.
RefSeqiNP_000841.1. NM_000850.4. [Q03013-1]
NP_671489.1. NM_147148.2. [Q03013-2]
UniGeneiHs.348387.

Genome annotation databases

EnsembliENST00000326729; ENSP00000316471; ENSG00000168765. [Q03013-2]
ENST00000369836; ENSP00000358851; ENSG00000168765. [Q03013-1]
GeneIDi2948.
KEGGihsa:2948.
UCSCiuc001dyf.4. human. [Q03013-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96234 mRNA. Translation: AAA57347.1.
M96233 Genomic DNA. Translation: AAA57346.1.
X68677 Genomic DNA. Translation: CAA48637.1.
M99422 mRNA. Translation: AAA58623.1.
DQ062813 mRNA. Translation: AAY98515.1.
CR541869 mRNA. Translation: CAG46667.1.
AK291880 mRNA. Translation: BAF84569.1.
AC000031 Genomic DNA. No translation available.
CH471122 Genomic DNA. Translation: EAW56405.1.
BC015513 mRNA. Translation: AAH15513.1.
BC108729 mRNA. Translation: AAI08730.1.
X56837 Genomic DNA. Translation: CAA40167.1.
CCDSiCCDS806.1. [Q03013-2]
CCDS807.1. [Q03013-1]
PIRiA47486.
S32425.
RefSeqiNP_000841.1. NM_000850.4. [Q03013-1]
NP_671489.1. NM_147148.2. [Q03013-2]
UniGeneiHs.348387.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GTUX-ray3.30A/B/C/D/E/F/G/H2-218[»]
ProteinModelPortaliQ03013.
SMRiQ03013.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109203. 14 interactors.
IntActiQ03013. 10 interactors.
MINTiMINT-1368381.
STRINGi9606.ENSP00000358851.

Chemistry databases

ChEMBLiCHEMBL2100.
DrugBankiDB00143. Glutathione.

PTM databases

iPTMnetiQ03013.
PhosphoSitePlusiQ03013.

Polymorphism and mutation databases

BioMutaiGSTM4.
DMDMi1170096.

2D gel databases

REPRODUCTION-2DPAGEIPI00008770.

Proteomic databases

EPDiQ03013.
MaxQBiQ03013.
PaxDbiQ03013.
PeptideAtlasiQ03013.
PRIDEiQ03013.

Protocols and materials databases

DNASUi2948.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000326729; ENSP00000316471; ENSG00000168765. [Q03013-2]
ENST00000369836; ENSP00000358851; ENSG00000168765. [Q03013-1]
GeneIDi2948.
KEGGihsa:2948.
UCSCiuc001dyf.4. human. [Q03013-1]

Organism-specific databases

CTDi2948.
DisGeNETi2948.
GeneCardsiGSTM4.
HGNCiHGNC:4636. GSTM4.
HPAiHPA055972.
HPA055973.
MIMi138333. gene.
neXtProtiNX_Q03013.
OpenTargetsiENSG00000168765.
PharmGKBiPA29026.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IN5J. Eukaryota.
ENOG4110YU0. LUCA.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115735.
HOVERGENiHBG106842.
InParanoidiQ03013.
KOiK00799.
OMAiAQPIRYL.
PhylomeDBiQ03013.
TreeFamiTF353040.

Enzyme and pathway databases

BioCyciZFISH:HS09817-MONOMER.
BRENDAi2.5.1.18. 2681.
ReactomeiR-HSA-156590. Glutathione conjugation.
SABIO-RKQ03013.

Miscellaneous databases

ChiTaRSiGSTM4. human.
EvolutionaryTraceiQ03013.
GeneWikiiGSTM4.
GenomeRNAii2948.
PROiQ03013.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168765.
CleanExiHS_GSTM4.
ExpressionAtlasiQ03013. baseline and differential.
GenevisibleiQ03013. HS.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01267. GSTRNSFRASEM.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTM4_HUMAN
AccessioniPrimary (citable) accession number: Q03013
Secondary accession number(s): A8K765
, Q05465, Q32NC1, Q4JNT8, Q6FH87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 170 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.