Q02ZS7 (FTHS_LACLS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	LACR_1007

Organism

Lactococcus lactis subsp. cremoris (strain SK11) [Complete proteome] [HAMAP]

Taxonomic identifier

272622 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. cremoris

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000300526
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02ZS7 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 53885E0E511816D1	FASTA	555	59,489
	10 20 30 40 50 60 MKTDIEIAQA AEIQPITKIA EKIGLSFDDI ELYGKYKAKI PLEVLEKFDE QTDGKLVLVT 70 80 90 100 110 120 SINPTPAGEG KSTVTVGLAD AFARQDKNVM VALREPSLGP VMGIKGGAAG GGFAQVLPME 130 140 150 160 170 180 DINLHFTGDI HAITTANNAI SAFLDNSLHQ GNPLNIDPRR IIWKRVLDLN DRALRHVTIG 190 200 210 220 230 240 LGGPLNGVPR EDGFDITVAS EIMAVLCLAT SISDLKERLA RIVIAQNYDR KPVSVGDLGV 250 260 270 280 290 300 QGAIAMLLKD ALKPNLVQTI EGTPALIHGG PFANIAHGCN SVLATKTALK LADIVITEAG 310 320 330 340 350 360 FGADLGGEKF LDIKTRQLGK QPDAVVVVAT LRALKMHGGV DKKELTSENV EAVKKGFANL 370 380 390 400 410 420 ERHIKNMQSY GLPVIVAINQ FASDTESEIS TLKELTEALG VSVSLTQVFA KGGEGGLDLA 430 440 450 460 470 480 EKLSAMLQAK PDFRYLYELN QPLSVKLDKV VTEIYGGSKV NLSPKAKRQM REIEENGWNN 490 500 510 520 530 540 LPVCMAKTQY SFSDQANLLA APEGFEVTVR ELIPKIGAGF IVALLGDVMT MPGLPKNPAA 550 LKMDVTDDGK ISGLF

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SK11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000425 Genomic DNA. Translation: ABJ72545.1.
RefSeq	YP_808967.1. NC_008527.1.
3D structure databases
ProteinModelPortal	Q02ZS7.
SMR	Q02ZS7. Positions 4-553.
ModBase	Search...
Protein-protein interaction databases
STRING	272622.LACR_1007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ72545; ABJ72545; LACR_1007.
GeneID	4432775.
KEGG	llc:LACR_1007.
PATRIC	22288839. VBILacLac38071_1168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	EIMAVLC.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	LLAC272622:GJUG-1007-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_LACLS

Accession

Primary (citable) accession number: Q02ZS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	November 14, 2006
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program