Q02Z38 (GUAC_LACLS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP reductase
EC=1.7.1.7

Alternative name(s):
Guanosine 5'-monophosphate oxidoreductase
Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	LACR_1256

Organism

Lactococcus lactis subsp. cremoris (strain SK11) [Complete proteome] [HAMAP]

Taxonomic identifier

272622 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	GMP reductase HAMAP MF_01511		PRO_0000294276
Regions
	Nucleotide binding	207 – 230	24	NADP Potential
Sites
	Active site	178	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02Z38 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: D11BBDD5203C4633	FASTA	329	35,828
	10 20 30 40 50 60 MNNLNSVFDY EDIQLIPNKC VINSRSEADT SVKLGNYTFK LPVVPANMQT IIDDKIAEML 70 80 90 100 110 120 AKEGYFYIMH RFEAENRAAF IKKMHKDGLI ASISVGVKAD EHAFIREISA EALIPEFITI 130 140 150 160 170 180 DIAHGHADSV IKTIQLIKRL MPQTFVIAGN VGTPEAVREL ENAGADATKV GIGPGKVCIT 190 200 210 220 230 240 KVKTGFGTGG WQLAAVKWCA KAASKPVIAD GGIRTHGDIA KSIRMGATMV MVGSLFAAHE 250 260 270 280 290 300 ESPGQTVERD GQLFKEYFGS ASEYQKGEHK NVEGKKILLP HKGSLKDTLK EMEEDLQSSI 310 320 SYAGGRDLSA LTKVDYVVVK NSIWNGDAI

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SK11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000425 Genomic DNA. Translation: ABJ72784.1.
RefSeq	YP_809206.1. NC_008527.1.
3D structure databases
ProteinModelPortal	Q02Z38.
SMR	Q02Z38. Positions 4-324.
ModBase	Search...
Protein-protein interaction databases
STRING	Q02Z38.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4433255.
GenomeReviews	Gene locus LACR_1256 in contig CP000425_GR.
KEGG	llc:LACR_1256.
PATRIC	22289351. VBILacLac38071_1422.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0516.
HOGENOM	HBG298985.
OMA	PDYITID.
ProtClustDB	PRK05458.
Enzyme and pathway databases
BioCyc	LLAC272622:LACR_1256-MONOMER.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00364.
PANTHER	PTHR11911:SF30. PTHR11911:SF30. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_LACLS

Accession

Primary (citable) accession number: Q02Z38

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program