Q02Y14 (HEMH_LACLS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	LACR_1661

Organism

Lactococcus lactis subsp. cremoris (strain SK11) [Complete proteome] [HAMAP]

Taxonomic identifier

272622 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Ferrochelatase HAMAP MF_00323		PRO_1000019313
Sites
	Metal binding	186	1	Iron By similarity
	Metal binding	268	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02Y14 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 7631925F2D735FEE	FASTA	318	36,986
	10 20 30 40 50 60 MDKKKGILLV ALGTPRSCET EDVREYLKEF LGDPLVIQKP RWLWLPILNG IILKVRPQKS 70 80 90 100 110 120 AEMYKQIWTD EGSPLMSYTI AQTEQLQGLR EDFDVRFAMT YGEPRIDKVI REMKESGVED 130 140 150 160 170 180 ITVLPLYPQY SLTTVEPIIQ QVKKIDDKIN VIRDFHQIES YTDLLAESIR EKWQANHYDK 190 200 210 220 230 240 LILSYHGIPL SYVTKKKDAY EAQCIETTRL VVEKLGLKEE EYEHTYQSKF GPEKWLEPAT 250 260 270 280 290 300 IDRIAELPKE DTKKVLICSP AFVADCLETL FELEIENKEV FVENGGETFD FVHPFNDSLE 310 FTKVLSEVIE KNKVEVEV

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SK11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000425 Genomic DNA. Translation: ABJ73158.1.
RefSeq	YP_809580.1. NC_008527.1.
3D structure databases
ProteinModelPortal	Q02Y14.
ModBase	Search...
Protein-protein interaction databases
STRING	Q02Y14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4433178.
GenomeReviews	Gene locus LACR_1661 in contig CP000425_GR.
KEGG	llc:LACR_1661.
PATRIC	22290195. VBILacLac38071_1843.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0276.
HOGENOM	HBG697135.
OMA	TIEEIGM.
ProtClustDB	CLSK697807.
Enzyme and pathway databases
BioCyc	LLAC272622:LACR_1661-MONOMER.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_LACLS

Accession

Primary (citable) accession number: Q02Y14

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program