Q02XB7 (AROE_LACLS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	LACR_1921

Organism

Lactococcus lactis subsp. cremoris (strain SK11) [Complete proteome] [HAMAP]

Taxonomic identifier

272622 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000021293
Regions
	Nucleotide binding	131 – 135	5	NADP By similarity
Sites
	Active site	71	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q02XB7 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 780C15B5CBBD6ECA	FASTA	286	31,234
	10 20 30 40 50 60 MKIDGYTRMA AVVANPIKHS LSPFIHNLAF DLTDENGVYL AWEVESKKLA AIVENVRNLD 70 80 90 100 110 120 MYGLNISMPY KGEIIKFMDE LSPAAELIGA VNTVVNHSGK IIGHNTDGIG FFNSLKKYDF 130 140 150 160 170 180 KIENKQMLVL GGGGAAIALI AQAALSGAKK IVVAARKSAS YDPLNEKLAK LSAKTGVEIF 190 200 210 220 230 240 LTDLSGADRL QKELNQTDLL VNATSVGMDG ASFPLEKSLL LPDRLLVVDA IYKVRETPFL 250 260 270 280 RWAKEQGAQT ENGLGMLIGQ AAESFYLWTG KEMPVDKITL EMEREV

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SK11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000425 Genomic DNA. Translation: ABJ73405.1.
RefSeq	YP_811518.1. NC_008527.1.
3D structure databases
ProteinModelPortal	Q02XB7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q02XB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4433895.
GenomeReviews	Gene locus LACR_1921 in contig CP000425_GR.
KEGG	llc:LACR_1921.
PATRIC	22290709. VBILacLac38071_2097.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	AWEVESK.
ProtClustDB	PRK00258.
Enzyme and pathway databases
BioCyc	LLAC272622:LACR_1921-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. AroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_LACLS

Accession

Primary (citable) accession number: Q02XB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program