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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74S-adenosyl-L-methionineUniRule annotation1
Binding sitei99S-adenosyl-L-methionineUniRule annotation1
Binding sitei126S-adenosyl-L-methionineUniRule annotation1
Active sitei149By similarity1
Binding sitei149S-adenosyl-L-methionineUniRule annotation1
Binding sitei153SubstrateUniRule annotation1
Binding sitei185SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPAER208963:G1G74-416-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:PA14_05000
OrganismiPseudomonas aeruginosa (strain UCBPP-PA14)
Taxonomic identifieri208963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000653 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002882031 – 244tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST244

Structurei

3D structure databases

ProteinModelPortaliQ02U31
SMRiQ02U31
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni222 – 225Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073968
KOiK03439

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q02U31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTPQSPAQ GSLAEHDEAR PMRTVKSFVM RAGRMTEGQQ RGLDLGWPKF
60 70 80 90 100
GLELSDEVQD FDAIFGRQAP RTFEIGFGMG HSTLEMAAAA PDIDFIGVEV
110 120 130 140 150
HKPGVGALLN GLLTQGLGNV RVYSCDALEV LRHCVADASL DRLLLFFPDP
160 170 180 190 200
WHKKRHHKRR IVQPEFAELV RRKLKVGGVL HMATDWEPYA EHMLDVMSAA
210 220 230 240
PGYRNQAEDG RFVARPQERP VTKFERRGER LGHGVWDLKF ERID
Length:244
Mass (Da):27,525
Last modified:May 29, 2007 - v2
Checksum:iE7DFE8529BE6FF56
GO

Sequence cautioni

The sequence ABJ15348 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000438 Genomic DNA Translation: ABJ15348.1 Different initiation.
RefSeqiWP_015502217.1, NC_008463.1

Genome annotation databases

EnsemblBacteriaiABJ15348; ABJ15348; PA14_05000
KEGGipau:PA14_05000

Similar proteinsi

Entry informationi

Entry nameiTRMB_PSEAB
AccessioniPrimary (citable) accession number: Q02U31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 64 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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