UniProtKB - Q02TT1 (MASZ_PSEAB)
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Protein
Malate synthase G
Gene
glcB
Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Status
Functioni
Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation
Catalytic activityi
Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation
Cofactori
Mg2+UniRule annotation
: glyoxylate cycle Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Malate synthase G (glcB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 118 | Acetyl-CoA; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 276 | Acetyl-CoAUniRule annotation | 1 | |
Binding sitei | 313 | Acetyl-CoAUniRule annotation | 1 | |
Active sitei | 340 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 340 | GlyoxylateUniRule annotation | 1 | |
Metal bindingi | 432 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 432 | GlyoxylateUniRule annotation | 1 | |
Metal bindingi | 460 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 541 | Acetyl-CoA; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 631 | Proton donorUniRule annotation | 1 |
GO - Molecular functioni
- malate synthase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- glyoxylate cycle Source: UniProtKB-UniPathway
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00703; UER00720. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:glcBUniRule annotation Ordered Locus Names:PA14_06290 |
Organismi | Pseudomonas aeruginosa (strain UCBPP-PA14) |
Taxonomic identifieri | 208963 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000056918 | 1 – 725 | Malate synthase GAdd BLAST | 725 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 617 | Cysteine sulfenic acid (-SOH)UniRule annotation | 1 |
Keywords - PTMi
OxidationInteractioni
Subunit structurei
Monomer.UniRule annotation
Structurei
3D structure databases
ProteinModelPortali | Q02TT1. |
SMRi | Q02TT1. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 125 – 126 | Acetyl-CoA bindingUniRule annotation | 2 | |
Regioni | 457 – 460 | Glyoxylate bindingUniRule annotation | 4 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | HOG000220740. |
KOi | K01638. |
OMAi | TEPVLHA. |
Family and domain databases
CDDi | cd00728. malate_synt_G. 1 hit. |
Gene3Di | 2.170.170.11. 1 hit. |
HAMAPi | MF_00641. Malate_synth_G. 1 hit. |
InterProi | View protein in InterPro IPR011076. Malate_synth-like_sf. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. |
Pfami | View protein in Pfam PF01274. Malate_synthase. 1 hit. |
SUPFAMi | SSF51645. SSF51645. 1 hit. |
TIGRFAMsi | TIGR01345. malate_syn_G. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q02TT1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTERVQVGGL QVAKVLFDFV NNEAIPGTGV SADTFWTGAE AVINDLAPKN
60 70 80 90 100
KALLAKRDEL QAKIDGWHQA RAGQAHDAAA YKAFLEEIGY LLPEAEDFQA
110 120 130 140 150
GTQNVDDEIA RMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDVISE
160 170 180 190 200
EGGAEKGKGY NKVRGDKVIA FARAFLDEAA PLESGSHVDA TSYSVKNGAL
210 220 230 240 250
VVALKNGSET GLKNAGQFLA FQGDAAKPQA VLLKHNGLHF EIQIDPSSPV
260 270 280 290 300
GQTDAAGVKD VLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL
310 320 330 340 350
AEEVSKGGST FTRTMNPDRV YTRADGSELT LHGRSLLFVR NVGHLMTNDA
360 370 380 390 400
ILDKDGNEVP EGIQDGLFTS LIAIHDLNGN TSRKNSRTGS VYIVKPKMHG
410 420 430 440 450
PEEAAFTNEL FGRVEDVLGL PRNTLKVGIM DEERRTTVNL KACIKAAKDR
460 470 480 490 500
VVFINTGFLD RTGDEIHTSM EAGAVVRKGA MKSEKWIGAY ENNNVDVGLA
510 520 530 540 550
TGLQGKAQIG KGMWAMPDLM AAMLEQKIGH PLAGANTAWV PSPTAATLHA
560 570 580 590 600
LHYHKVDVFA RQAELAKRTP ASVDDILTIP LAPNTNWTAE EIKNEVDNNA
610 620 630 640 650
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ LLANWLRHGV
660 670 680 690 700
ISQEQVVESL KRMAVVVDRQ NASDPSYRPM APNFDDNVAF QAALELVVEG
710 720
TRQPNGYTEP VLHRRRREFK AKNGL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000438 Genomic DNA. Translation: ABJ15447.1. |
RefSeqi | WP_003105217.1. NC_008463.1. |
Genome annotation databases
EnsemblBacteriai | ABJ15447; ABJ15447; PA14_06290. |
KEGGi | pau:PA14_06290. |
Similar proteinsi
Entry informationi
Entry namei | MASZ_PSEAB | |
Accessioni | Q02TT1Primary (citable) accession number: Q02TT1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | November 14, 2006 | |
Last modified: | March 28, 2018 | |
This is version 75 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |