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Protein

Malate synthase G

Gene

glcB

Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei276Acetyl-CoAUniRule annotation1
Binding sitei313Acetyl-CoAUniRule annotation1
Active sitei340Proton acceptorUniRule annotation1
Binding sitei340GlyoxylateUniRule annotation1
Metal bindingi432MagnesiumUniRule annotation1
Binding sitei432GlyoxylateUniRule annotation1
Metal bindingi460MagnesiumUniRule annotation1
Binding sitei541Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei631Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:PA14_06290
OrganismiPseudomonas aeruginosa (strain UCBPP-PA14)
Taxonomic identifieri208963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000653 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000569181 – 725Malate synthase GAdd BLAST725

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei617Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ02TT1
SMRiQ02TT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni457 – 460Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000220740
KOiK01638
OMAiTEPVLHA

Family and domain databases

CDDicd00728 malate_synt_G, 1 hit
Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

Q02TT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERVQVGGL QVAKVLFDFV NNEAIPGTGV SADTFWTGAE AVINDLAPKN
60 70 80 90 100
KALLAKRDEL QAKIDGWHQA RAGQAHDAAA YKAFLEEIGY LLPEAEDFQA
110 120 130 140 150
GTQNVDDEIA RMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDVISE
160 170 180 190 200
EGGAEKGKGY NKVRGDKVIA FARAFLDEAA PLESGSHVDA TSYSVKNGAL
210 220 230 240 250
VVALKNGSET GLKNAGQFLA FQGDAAKPQA VLLKHNGLHF EIQIDPSSPV
260 270 280 290 300
GQTDAAGVKD VLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL
310 320 330 340 350
AEEVSKGGST FTRTMNPDRV YTRADGSELT LHGRSLLFVR NVGHLMTNDA
360 370 380 390 400
ILDKDGNEVP EGIQDGLFTS LIAIHDLNGN TSRKNSRTGS VYIVKPKMHG
410 420 430 440 450
PEEAAFTNEL FGRVEDVLGL PRNTLKVGIM DEERRTTVNL KACIKAAKDR
460 470 480 490 500
VVFINTGFLD RTGDEIHTSM EAGAVVRKGA MKSEKWIGAY ENNNVDVGLA
510 520 530 540 550
TGLQGKAQIG KGMWAMPDLM AAMLEQKIGH PLAGANTAWV PSPTAATLHA
560 570 580 590 600
LHYHKVDVFA RQAELAKRTP ASVDDILTIP LAPNTNWTAE EIKNEVDNNA
610 620 630 640 650
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ LLANWLRHGV
660 670 680 690 700
ISQEQVVESL KRMAVVVDRQ NASDPSYRPM APNFDDNVAF QAALELVVEG
710 720
TRQPNGYTEP VLHRRRREFK AKNGL
Length:725
Mass (Da):78,632
Last modified:November 14, 2006 - v1
Checksum:iCEC503219946E5CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000438 Genomic DNA Translation: ABJ15447.1
RefSeqiWP_003105217.1, NC_008463.1

Genome annotation databases

EnsemblBacteriaiABJ15447; ABJ15447; PA14_06290
KEGGipau:PA14_06290

Similar proteinsi

Entry informationi

Entry nameiMASZ_PSEAB
AccessioniPrimary (citable) accession number: Q02TT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: April 25, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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