glcB

Functionⁱ

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityⁱ

Acetyl-CoA + H₂O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactorⁱ

Mg²⁺UniRule annotation

Pathwayⁱ: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

no protein annotated in this organism
Malate synthase G (glcB)

This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	118	Acetyl-CoA; via carbonyl oxygenUniRule annotation	1
Binding siteⁱ	276	Acetyl-CoAUniRule annotation	1
Binding siteⁱ	313	Acetyl-CoAUniRule annotation	1
Active siteⁱ	340	Proton acceptorUniRule annotation	1
Binding siteⁱ	340	GlyoxylateUniRule annotation	1
Metal bindingⁱ	432	MagnesiumUniRule annotation	1
Binding siteⁱ	432	GlyoxylateUniRule annotation	1
Metal bindingⁱ	460	MagnesiumUniRule annotation	1
Binding siteⁱ	541	Acetyl-CoA; via carbonyl oxygenUniRule annotation	1
Active siteⁱ	631	Proton donorUniRule annotation	1

GO - Molecular functionⁱ

malate synthase activity Source: UniProtKB-EC
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

glyoxylate cycle Source: UniProtKB-UniPathway
tricarboxylic acid cycle Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Glyoxylate bypass, Tricarboxylic acid cycle
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00703; UER00720.

UniPathwayⁱ

UPA00703; UER00720.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesⁱ	Name:glcBUniRule annotation Ordered Locus Names:PA14_06290
Organismⁱ	Pseudomonas aeruginosa (strain UCBPP-PA14)
Taxonomic identifierⁱ	208963 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa
Proteomesⁱ	UP000000653 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00641

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_1000056918	1 – 725	Malate synthase GAdd BLAST		725

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	617	Cysteine sulfenic acid (-SOH)UniRule annotation		1

Keywords - PTMⁱ

Oxidation

Interactionⁱ

Subunit structureⁱ

Monomer.UniRule annotation

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q02TT1.
SMRⁱ	Q02TT1.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	125 – 126	Acetyl-CoA bindingUniRule annotation		2
Regionⁱ	457 – 460	Glyoxylate bindingUniRule annotation		4

Sequence similaritiesⁱ

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMⁱ	HOG000220740.
KEGG Orthology (KO) More...KOⁱ	K01638.
OMAⁱ	TEPVLHA.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00728. malate_synt_G. 1 hit.
Gene3Dⁱ	2.170.170.11. 1 hit.
HAMAPⁱ	MF_00641. Malate_synth_G. 1 hit.
InterProⁱ	View protein in InterPro IPR011076. Malate_synth-like_sf. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01274. Malate_synthase. 1 hit.
SUPFAMⁱ	SSF51645. SSF51645. 1 hit.
TIGRFAMsⁱ	TIGR01345. malate_syn_G. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q02TT1-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTERVQVGGL QVAKVLFDFV NNEAIPGTGV SADTFWTGAE AVINDLAPKN 
        60         70         80         90        100
KALLAKRDEL QAKIDGWHQA RAGQAHDAAA YKAFLEEIGY LLPEAEDFQA 
       110        120        130        140        150
GTQNVDDEIA RMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDVISE 
       160        170        180        190        200
EGGAEKGKGY NKVRGDKVIA FARAFLDEAA PLESGSHVDA TSYSVKNGAL 
       210        220        230        240        250
VVALKNGSET GLKNAGQFLA FQGDAAKPQA VLLKHNGLHF EIQIDPSSPV 
       260        270        280        290        300
GQTDAAGVKD VLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL 
       310        320        330        340        350
AEEVSKGGST FTRTMNPDRV YTRADGSELT LHGRSLLFVR NVGHLMTNDA 
       360        370        380        390        400
ILDKDGNEVP EGIQDGLFTS LIAIHDLNGN TSRKNSRTGS VYIVKPKMHG 
       410        420        430        440        450
PEEAAFTNEL FGRVEDVLGL PRNTLKVGIM DEERRTTVNL KACIKAAKDR 
       460        470        480        490        500
VVFINTGFLD RTGDEIHTSM EAGAVVRKGA MKSEKWIGAY ENNNVDVGLA 
       510        520        530        540        550
TGLQGKAQIG KGMWAMPDLM AAMLEQKIGH PLAGANTAWV PSPTAATLHA 
       560        570        580        590        600
LHYHKVDVFA RQAELAKRTP ASVDDILTIP LAPNTNWTAE EIKNEVDNNA 
       610        620        630        640        650
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ LLANWLRHGV 
       660        670        680        690        700
ISQEQVVESL KRMAVVVDRQ NASDPSYRPM APNFDDNVAF QAALELVVEG 
       710        720 
TRQPNGYTEP VLHRRRREFK AKNGL

Length:725

Mass (Da):78,632

Last modified:November 14, 2006 - v1

Checksum:ⁱCEC503219946E5CE

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000438 Genomic DNA. Translation: ABJ15447.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_003105217.1. NC_008463.1.

Genome annotation databases

EnsemblBacteriaⁱ	ABJ15447; ABJ15447; PA14_06290.
KEGGⁱ	pau:PA14_06290.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q02TT1	B7V467 A0A1E9BXM6 A0A157U7E7 A0A0C7D2Q3 A0A0H3QXW6 A0A1S1C1W1 A0A1F0IWE0 UPI00066A61D8 UPI00066C1A37	Pseudomonas aeruginosa (strain LESB58) Pseudomonas sp. HMSC066B11 Enterobacter cloacae Pseudomonas aeruginosa Pseudomonas aeruginosa str. Stone 130 Pseudomonas sp. HMSC059F05 Pseudomonas sp. HMSC065H01	725	UniRef100_B7V467	Cluster: Malate synthase G	10

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q02TT1	Q9I636 A0A2K0BGT6 UPI0009FB94D8 UPI00053D4407 UPI00066A3A77 UPI000BEE81E3 A0A1B8TBW7 A0A1A9K5H1 UPI000772814B UPI000937BD29 +127	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) Pseudomonas aeruginosa Pseudomonas sp. AU12215 Pseudomonas citronellolis Acinetobacter baumannii Pseudomonas sp. AAC Pseudomonas sp. HMSC75E02 Pseudomonas sp. AU11447 Pseudomonas sp. EGD-AKN5 Pseudomonas sp. (strain ADP) And more	725	UniRef90_Q9I636	Cluster: Malate synthase G	138

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q02TT1	P42450 A0A2H5IAV3 A0A172QV25 A0A160PR55 A0A1R4FXK5 UPI00058A5BC2 UPI00080749FF UPI0009450C97 A0A0U4WVM8 UPI0004F86503 +3661	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) Corynebacterium glutamicum (Brevibacterium saccharolyticum) Corynebacterium crudilactis Corynebacterium Corynebacterium glutamicum (strain R) Corynebacterium crenatum MT Corynebacterium glutamicum ATCC 14067 [Brevibacterium] flavum Corynebacterium deserti GIMN1.010 Paenibacillus ginsengihumi And more	739	UniRef50_P42450	Cluster: Malate synthase G	3,672

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000438 Genomic DNA. Translation: ABJ15447.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_003105217.1. NC_008463.1.

3D structure databases

ProteinModelPortalⁱ	Q02TT1.
SMRⁱ	Q02TT1.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ABJ15447; ABJ15447; PA14_06290.
KEGGⁱ	pau:PA14_06290.

Phylogenomic databases

HOGENOMⁱ	HOG000220740.
KEGG Orthology (KO) More...KOⁱ	K01638.
OMAⁱ	TEPVLHA.

Enzyme and pathway databases

UniPathwayⁱ	UPA00703; UER00720.

UniPathwayⁱ

UPA00703; UER00720.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00728. malate_synt_G. 1 hit.
Gene3Dⁱ	2.170.170.11. 1 hit.
HAMAPⁱ	MF_00641. Malate_synth_G. 1 hit.
InterProⁱ	View protein in InterPro IPR011076. Malate_synth-like_sf. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01274. Malate_synthase. 1 hit.
SUPFAMⁱ	SSF51645. SSF51645. 1 hit.
TIGRFAMsⁱ	TIGR01345. malate_syn_G. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MASZ_PSEAB
Accessionⁱ	Q02TT1Primary (citable) accession number: Q02TT1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
	Last sequence update:	November 14, 2006
	Last modified:	March 28, 2018
	This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q02TT1 (MASZ_PSEAB)

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Malate synthase G

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glyoxylate cycle

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: glyoxylate cycle

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ