Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q02T18 (Q02T18_PSEAB) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL ABJ13474.1
Synonyms:ddl HAMAP-Rule MF_00047
Ordered Locus Names:PA14_09600 EMBL ABJ13474.1
OrganismPseudomonas aeruginosa (strain UCBPP-PA14) [Complete proteome] [HAMAP] EMBL ABJ13474.1
Taxonomic identifier208963 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain138 – 332195ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding164 – 21754ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2861Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2991Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2991Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3011Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
Q02T18 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: A66A99C9B74958E7

FASTA34636,476
        10         20         30         40         50         60 
MGQDKLKVAV LFGGSSEERD VSIASGAQVI QALRSAGHQV LAVDTASGLL GAEEERRLLA 

        70         80         90        100        110        120 
SKVKEVPPDS DSLAIIRSGK QSLLSAGELA GVDVFFLALH GGTGEDGTLQ ALLDAGGFAY 

       130        140        150        160        170        180 
TGSGHLASAM AMDKDVAKRL FLAAGVETAS WLMAPASEEA VREQLGFPLV VKPNSQGSTV 

       190        200        210        220        230        240 
GLSIVHSQAE LQPAIELAGR YGDEVMLERF VAGREVTVGV LDDQALPVGE ILLGGQEVFD 

       250        260        270        280        290        300 
YEHKYQAGAV REVFPADLPP AIAAEAQRLA LKVHRALKLS GYSRTDFRLD EQGRLWCLEV 

       310        320        330        340 
NTLPGMTATS LLPQAAAAAG IGFAELCERI CRLGIERCKG ARKARS 

« Hide

References

[1]"Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial."
Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S., Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G., Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.
Genome Biol. 7:R90.1-R90.14(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: UCBPP-PA14 EMBL ABJ13474.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000438 Genomic DNA. Translation: ABJ13474.1.
RefSeqYP_788918.1. NC_008463.1.

3D structure databases

ProteinModelPortalQ02T18.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208963.PA14_09600.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ13474; ABJ13474; PA14_09600.
GeneID4382211.
KEGGpau:PA14_09600.
PATRIC19847618. VBIPseAer79785_0782.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADYTARYT.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycPAER208963:GI5K-775-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ02T18_PSEAB
AccessionPrimary (citable) accession number: Q02T18
Entry history
Integrated into UniProtKB/TrEMBL: November 14, 2006
Last sequence update: November 14, 2006
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)