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Protein

Glycerol-3-phosphate acyltransferase

Gene

plsB

Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.UniRule annotation

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase (plsB)
  2. no protein annotated in this organism
  3. Phosphatidate cytidylyltransferase (cdsA)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciPAER208963:GI5K-1355-MONOMER.
UniPathwayiUPA00557; UER00612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferaseUniRule annotation (EC:2.3.1.15UniRule annotation)
Short name:
GPATUniRule annotation
Gene namesi
Name:plsBUniRule annotation
Ordered Locus Names:PA14_16860
OrganismiPseudomonas aeruginosa (strain UCBPP-PA14)
Taxonomic identifieri208963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000653 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 834834Glycerol-3-phosphate acyltransferasePRO_1000049443Add
BLAST

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi309 – 3146HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.UniRule annotation

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans. 1 hit.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02RE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRYPFRRFG FGALRRLLYL WVRSETINQS AFTLKIDRSK PVLYVLQQPS
60 70 80 90 100
VSDLAVVDTE CRKAGLPRPV MPVAVGDAIE PAAFFYLTPE PDWLGRQDKR
110 120 130 140 150
GASPTLVRML AAVGQNGLDD AQIIPVSVFW GQSPDSESSP WKLLFADNWA
160 170 180 190 200
VTGRLRKLAR ILILGRKTRV QFSAPIHLRE LVEQGKGHER TLRMVNRILR
210 220 230 240 250
VHFRNLKTAV IGPDLSHRRN LVKGLLRAPL VRQAINEECE SERISQEKAE
260 270 280 290 300
GIALRYANEI ASDFSYPVIR FLEVILSWFW NKLYEGVKVN HIERVQDVAQ
310 320 330 340 350
GNEIVYVPCH RSHIDYLLLS YLLFRNGLTP PHIAAGINLN MPVIGSILRR
360 370 380 390 400
GGAFFMRRSF KGNQLYTAVF NEYLHTLFSR GFSTEYFVEG GRSRTGRMLH
410 420 430 440 450
PRTGMLAITL RSFLRDSRRP IVFVPVYIGY ERVLEGRTYL GELRGATKKK
460 470 480 490 500
ESIFDLFKVV GALKQRFGQV WVNFGEPIHL DQFLDRHQPD WQDQDLGPEY
510 520 530 540 550
RPDWLPQTTN LLAKDVARHL NDAAAINPVN LVALALLSTS RQALDESALA
560 570 580 590 600
RILDLYLALL RKVPYSPSAT LPDGDGQALI EYVKSMNLLA EQKDALGRIL
610 620 630 640 650
YLDEQNAVLA TYYRNNVLHV FALPALIASF FQSNSRISRE QLLRFARALY
660 670 680 690 700
PYLQAELFIR WSLDELDAVI DQWLAALVEQ GLLRQENDTF IRPAPSSRQY
710 720 730 740 750
VLLILLARSV TQTLQRFYMA IALLLNAGQN ALTAEELENL CTVMAQRLSI
760 770 780 790 800
LHGLNAPEFF DKSLFRHFIQ TLLDLRVLRK DEAGKLSYHE LLGELAEGAA
810 820 830
KRVLPAEIRL SIRQVALERP AEEAAAESND AAAN
Length:834
Mass (Da):94,785
Last modified:November 14, 2006 - v1
Checksum:iE7CFFEFC2E39431F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000438 Genomic DNA. Translation: ABJ12906.1.
RefSeqiWP_003092456.1. NC_008463.1.

Genome annotation databases

EnsemblBacteriaiABJ12906; ABJ12906; PA14_16860.
KEGGipau:PA14_16860.
PATRICi19848799. VBIPseAer79785_1371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000438 Genomic DNA. Translation: ABJ12906.1.
RefSeqiWP_003092456.1. NC_008463.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ12906; ABJ12906; PA14_16860.
KEGGipau:PA14_16860.
PATRICi19848799. VBIPseAer79785_1371.

Phylogenomic databases

HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.
BioCyciPAER208963:GI5K-1355-MONOMER.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans. 1 hit.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLSB_PSEAB
AccessioniPrimary (citable) accession number: Q02RE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: September 7, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.