Reviewed, UniProtKB/Swiss-Prot Q02QW0 (MAO1_PSEAB)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	PA14_19190

Organism

Pseudomonas aeruginosa (strain UCBPP-PA14) [Complete proteome] [HAMAP]

Taxonomic identifier

208963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	NAD-dependent malic enzyme		PRO_1000069534
Sites
	Active site	102	1	Proton donor By similarity
	Active site	173	1	Proton acceptor By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	245	1	Divalent metal cation By similarity
	Metal binding	268	1	Divalent metal cation By similarity
	Binding site	155	1	NAD By similarity
	Binding site	268	1	NAD By similarity
	Binding site	417	1	NAD By similarity
	Site	268	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02QW0-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: C11DD287882BA4AD	FASTA	564	62,448
	10 20 30 40 50 60 MTETAKRPLY VPHAGPSLLE MPLLNKGSAF STQERIDFNL QGLLPHNIET IEEQTERAYS 70 80 90 100 110 120 QYNLCNTDLD RHIFLRSIQD NNETLFFRLL EEHLEEMMPI IYTPTVGQAC QEFSKIYRTH 130 140 150 160 170 180 RGLFISYPDR ERIDDILRSA TKNNVKIVVV TDSERILGLG DQGIGGMGIP IGKLSLYTAC 190 200 210 220 230 240 GGISPAYTLP VVLDVGTNNP DLLNDPMYMG WRHERVSGAQ YEEFVDLFIQ AIKRRWPNVL 250 260 270 280 290 300 LQFEDFAQTN AMPLLERYKD ELCCFNDDIQ GTAAVAVGTL LAACKAKGEK LSEQTVTFVG 310 320 330 340 350 360 AGSAGCGIAE QIIAAMQLEG LDEAQARRRI FMVDRWGLLT DDMSNLLDFQ HRLAQKRADL 370 380 390 400 410 420 GAWGGQQGDD LALLEVIRNA RPTVLIGVSG QRGLFSEEVI RELHSHCKQP LVMPLSNPTS 430 440 450 460 470 480 RVEATPQEIL NWTDGQALVA TGSPFQPVQV GDKRIPIAQC NNAYIFPGIG LGVIAARANR 490 500 510 520 530 540 VTEGMLMAAA NALANCSPIV TRGEGAVLPA LGDIREVSKR IAVAVAKQAQ AEGKALHTSD 550 560 EVLNDAIEAN FWFPRYRAYR RTSF

References

[1]

"Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial." Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S., Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G., Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M. Genome Biol. 7:RESEARCH90.1-RESEARCH90.14(2006) [PubMed: 17038190] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000438 Genomic DNA. Translation: ABJ12726.1.
RefSeq	YP_789676.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4381436.
GenomeReviews	Gene locus PA14_19190 in contig CP000438_GR.
KEGG	pau:PA14_19190.
NMPDR	fig|208963.3.peg.1024.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_PSEAB

Accession

Primary (citable) accession number: Q02QW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 14, 2006
Last modified:	November 25, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)