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Q02QC9

- PPBH_PSEAB

UniProt

Q02QC9 - PPBH_PSEAB

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Protein

Alkaline phosphatase H

Gene

phoA

Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Has only phosphomonoesterase activity.By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg2+ ion per subunit
  • Zn2+Note: Binds 2 Zn2+ ions per subunit

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771MagnesiumBy similarity
Metal bindingi77 – 771Zinc 2By similarity
Active sitei128 – 1281Phosphoserine intermediate2 PublicationsPROSITE-ProRule annotation
Metal bindingi179 – 1791MagnesiumBy similarity
Metal bindingi181 – 1811MagnesiumBy similarity
Metal bindingi346 – 3461MagnesiumBy similarity
Metal bindingi353 – 3531Zinc 1By similarity
Metal bindingi357 – 3571Zinc 1By similarity
Metal bindingi395 – 3951Zinc 2By similarity
Metal bindingi396 – 3961Zinc 2By similarity
Metal bindingi438 – 4381Zinc 1By similarity

GO - Molecular functioni

  1. phosphatase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPAER208963:GI5K-1722-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase HPROSITE-ProRule annotation (EC:3.1.3.1PROSITE-ProRule annotation)
Alternative name(s):
High molecular weight phosphatase
Short name:
H-AP
Gene namesi
Name:phoA
Ordered Locus Names:PA14_21410
OrganismiPseudomonas aeruginosa (strain UCBPP-PA14)
Taxonomic identifieri208963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000653: Chromosome

Subcellular locationi

Secreted 1 Publication. Periplasm By similarity

Keywords - Cellular componenti

Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 476450Alkaline phosphatase HSequence AnalysisPRO_0000431467Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine2 Publications
Modified residuei206 – 2061Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi208963.PA14_21410.

Structurei

3D structure databases

ProteinModelPortaliQ02QC9.
SMRiQ02QC9. Positions 31-475.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099117.
KOiK01077.
OMAiCPSNALE.
OrthoDBiEOG661H4G.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02QC9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTPGYPLALS LAVSMAVLGS ALPAQARQDD PSLFNRQARG ELSEYGGARR
60 70 80 90 100
VEQDLTQALK QSLSKKKAKN VILLIGDGMG DSEITVARNY ARGAGGYFKG
110 120 130 140 150
IDALPLTGQY THYSLHKDSG LPDYVTDSAA SATAWTTGVK SYNGAIGVDI
160 170 180 190 200
HEQPHRNLLE LAKLNGKATG NVSTAELQDA TPAALLAHVT ARKCYGPEAT
210 220 230 240 250
SKQCPSNALE NGGAGSITEQ WLKTRPDVVL GGGAATFAET AKAGRYAGKT
260 270 280 290 300
LRAQAEARGY RIVENLDELK AVRRANQKQP LIGLFAPGNM PVRWLGPTAT
310 320 330 340 350
YHGNLNQPAV SCEANPKRTA DIPTLAQMTS KAIELLKDNP NGFFLQVEGA
360 370 380 390 400
SIDKQDHAAN PCGQIGETVD LDEAVQKALA FAKADGETLV IVTADHAHSS
410 420 430 440 450
QIIPPETAAP GLTQLLTTKD GAPLAISYGN SEEGSQEHTG TQLRIAAYGP
460 470
QAANVTGLTD QTDLFFTIRR ALNLRD
Length:476
Mass (Da):50,393
Last modified:November 14, 2006 - v1
Checksum:iCC4D5F2BCDADE688
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000438 Genomic DNA. Translation: ABJ12547.1.
RefSeqiYP_789857.1. NC_008463.1.

Genome annotation databases

EnsemblBacteriaiABJ12547; ABJ12547; PA14_21410.
GeneIDi4381315.
KEGGipau:PA14_21410.
PATRICi19849551. VBIPseAer79785_1746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000438 Genomic DNA. Translation: ABJ12547.1 .
RefSeqi YP_789857.1. NC_008463.1.

3D structure databases

ProteinModelPortali Q02QC9.
SMRi Q02QC9. Positions 31-475.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208963.PA14_21410.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ12547 ; ABJ12547 ; PA14_21410 .
GeneIDi 4381315.
KEGGi pau:PA14_21410.
PATRICi 19849551. VBIPseAer79785_1746.

Phylogenomic databases

eggNOGi COG1785.
HOGENOMi HOG000099117.
KOi K01077.
OMAi CPSNALE.
OrthoDBi EOG661H4G.

Enzyme and pathway databases

BioCyci PAER208963:GI5K-1722-MONOMER.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 2 hits.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UCBPP-PA14.
  2. "Potential of liquid-isoelectric-focusing protein fractionation to improve phosphoprotein characterization of Pseudomonas aeruginosa PA14."
    Ouidir T., Jarnier F., Cosette P., Jouenne T., Hardouin J.
    Anal. Bioanal. Chem. 406:6297-6309(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, PHOSPHORYLATION AT SER-128.
    Strain: UCBPP-PA14.
  3. "Extracellular Ser/Thr/Tyr phosphorylated proteins of Pseudomonas aeruginosa PA14 strain."
    Ouidir T., Jarnier F., Cosette P., Jouenne T., Hardouin J.
    Proteomics 14:2017-2030(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-128 AND SER-206.
    Strain: UCBPP-PA14.

Entry informationi

Entry nameiPPBH_PSEAB
AccessioniPrimary (citable) accession number: Q02QC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: November 14, 2006
Last modified: January 7, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 2 known alkaline phosphatase proteins in P.aeruginosa strain PAO1. The larger is well conserved in strain PA14 (this entry), but the other is not well conserved.Curated

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.