Reviewed, UniProtKB/Swiss-Prot Q02N70 (T23O_PSEAB)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	PA14_30750

Organism

Pseudomonas aeruginosa (strain UCBPP-PA14) [Complete proteome] [HAMAP]

Taxonomic identifier

208963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Tryptophan 2,3-dioxygenase		PRO_0000360123
Regions
	Region	32 – 36	5	Substrate binding By similarity
	Region	57 – 61	5	Substrate binding By similarity
Sites
	Metal binding	246	1	Iron (heme axial ligand) By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	130	1	Heme By similarity
	Binding site	260	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02N70-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 2CC7547E429C757F	FASTA	288	32,990
	10 20 30 40 50 60 MCPCPHSQAQ GETDGEAEWH NAALDFTHAM SYGDYLKLDK VLDAQFPLSP DHNEMLFIIQ 70 80 90 100 110 120 HQTSELWMKL MLHELRAARE HVKSGKLGPA LKMLARVSRI FDQLVHAWAV LATMTPTEYN 130 140 150 160 170 180 TIRPYLGQSS GFQSYQYREI EFILGNKNAT LLKPHAHRAE LLAALEQALH TPSLYDEAIR 190 200 210 220 230 240 LMAAQGLPVS QERLVRDAAA GTCYEASVEA AWRQVYQTPE RYWDLYQLAE KLIDLEDSFR 250 260 270 280 QWRFRHVTTV ERIIGFKPGT GGTEGVGYLR SMLDTILFPE LWRLRSNL

References

[1]

"Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial." Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S., Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G., Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M. Genome Biol. 7:R90.1-R90.14(2006) [PubMed: 17038190] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000438 Genomic DNA. Translation: ABJ11801.1.
RefSeq	YP_790616.1.
3D structure databases
SMR	Q02N70. Positions 30-288.
ModBase	Search...
Protein-protein interaction databases
STRING	Q02N70.
Genome annotation databases
GeneID	4380609.
GenomeReviews	Gene locus PA14_30750 in contig CP000438_GR.
KEGG	pau:PA14_30750.
NMPDR	fig|208963.3.peg.148.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3483.
HOGENOM	HBG647485.
OMA	QWSVLAT.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_PSEAB

Accession

Primary (citable) accession number: Q02N70

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	November 14, 2006
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)