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Protein

Kynureninase

Gene

kynU

Organism
Pseudomonas aeruginosa (strain UCBPP-PA14)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.UniRule annotation

Catalytic activityi

L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.UniRule annotation
L-kynurenine + H2O = anthranilate + L-alanine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • pyridoxal 5'-phosphateUniRule annotation
  • pyridoxal 5'-phosphateUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971Pyridoxal phosphate; via amide nitrogenUniRule annotation
Binding sitei98 – 981Pyridoxal phosphateUniRule annotation
Binding sitei201 – 2011Pyridoxal phosphateUniRule annotation
Binding sitei204 – 2041Pyridoxal phosphateUniRule annotation
Binding sitei226 – 2261Pyridoxal phosphateUniRule annotation
Binding sitei256 – 2561Pyridoxal phosphateUniRule annotation
Binding sitei282 – 2821Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

  1. kynureninase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-HAMAP
  2. anthranilate metabolic process Source: UniProtKB-HAMAP
  3. L-kynurenine catabolic process Source: UniProtKB-UniPathway
  4. quinolinate biosynthetic process Source: UniProtKB-HAMAP
  5. tryptophan catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation

Keywords - Biological processi

Pyridine nucleotide biosynthesisUniRule annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotation

Enzyme and pathway databases

BioCyciPAER208963:GI5K-3045-MONOMER.
UniPathwayiUPA00253; UER00329.

Names & Taxonomyi

Protein namesi
Recommended name:
KynureninaseUniRule annotation (EC:3.7.1.3UniRule annotation)
Alternative name(s):
L-kynurenine hydrolaseUniRule annotation
Gene namesi
Name:kynUUniRule annotation
Ordered Locus Names:PA14_37610Imported
OrganismiPseudomonas aeruginosa (strain UCBPP-PA14)Imported
Taxonomic identifieri208963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000653: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei227 – 2271N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi208963.PA14_37610.

Structurei

3D structure databases

ProteinModelPortaliQ02LM7.
SMRiQ02LM7. Positions 2-404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1324Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the kynureninase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3844.
HOGENOMiHOG000242437.
KOiK01556.
OMAiRFWQPLS.
OrthoDBiEOG6N67XP.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02LM7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTRDDCLAL DAGDPLADLR QLFALPDGVI YLDGNSLGAR PRAAVERAAE
60 70 80 90 100
VVAAEWGEGL IRSWNSADWR GLPERLGDKL APLIGARAGE VVITDTTSIN
110 120 130 140 150
LFKVLSAALR IQEEEAPGRK VIVSESSNFP TDLYIAEGLT DMLQRGYRLR
160 170 180 190 200
LVDGPEQLPA AIDADTAVVM LSHVNYKTGY LHDMREVTRL VHENGALAIW
210 220 230 240 250
DLAHSAGALP LDLHAAEADY AIGCTYKYLN GGPGSPAYVW VAPRLRERVW
260 270 280 290 300
QPLSGWFGHS RQFAMEPRYQ PGEGITRFLC GTQPITSLAL VECGLDIFAR
310 320 330 340 350
TDMQRLRDKS LALADLFIEL VESRCERFGL TLVTPREHAR RGSHVSFEHA
360 370 380 390 400
QGYAIVQALI DRGVIGDYRE PGILRFGFTP LYTRFVEVWD AVQALLEILQ
410
SEAWKEPRYQ VRHKVT
Length:416
Mass (Da):46,265
Last modified:November 14, 2006 - v1
Checksum:i105600F7FFCEC3D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000438 Genomic DNA. Translation: ABJ11263.1.
RefSeqiYP_791159.1. NC_008463.1.

Genome annotation databases

EnsemblBacteriaiABJ11263; ABJ11263; PA14_37610.
GeneIDi4380322.
KEGGipau:PA14_37610.
PATRICi19852254. VBIPseAer79785_3076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000438 Genomic DNA. Translation: ABJ11263.1.
RefSeqiYP_791159.1. NC_008463.1.

3D structure databases

ProteinModelPortaliQ02LM7.
SMRiQ02LM7. Positions 2-404.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208963.PA14_37610.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ11263; ABJ11263; PA14_37610.
GeneIDi4380322.
KEGGipau:PA14_37610.
PATRICi19852254. VBIPseAer79785_3076.

Phylogenomic databases

eggNOGiCOG3844.
HOGENOMiHOG000242437.
KOiK01556.
OMAiRFWQPLS.
OrthoDBiEOG6N67XP.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00329.
BioCyciPAER208963:GI5K-3045-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UCBPP-PA14Imported.

Entry informationi

Entry nameiQ02LM7_PSEAB
AccessioniPrimary (citable) accession number: Q02LM7
Entry historyi
Integrated into UniProtKB/TrEMBL: November 14, 2006
Last sequence update: November 14, 2006
Last modified: February 4, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.