Reviewed, UniProtKB/Swiss-Prot Q02LF6 (HGD_PSEAB)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	PA14_38510

Organism

Pseudomonas aeruginosa (strain UCBPP-PA14) [Complete proteome] [HAMAP]

Taxonomic identifier

208963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O(2) = 4-maleylacetoacetate.
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Homogentisate 1,2-dioxygenase		PRO_1000019533
Sites
	Metal binding	330	1	Iron By similarity
	Metal binding	336	1	Iron By similarity
	Metal binding	366	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02LF6-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 674D2DDAD42A149C	FASTA	432	47,851
	10 20 30 40 50 60 MNLDSTALAY QSGFGNEFSS EALPGALPVG QNSPQKAPYG LYAELLSGTA FTMARSEARR 70 80 90 100 110 120 TWLYRITPSA KHPPFRRLER QIAGAELDAP TPNRLRWDPL ALPEQPTDFL DGLLRMAANA 130 140 150 160 170 180 PGDKPAGVSI YQYLANRSME RCFYDADGEL LLVPQLGRLR LCTELGALQV EPLEIAVIPR 190 200 210 220 230 240 GMKFRVELLD GEARGYIAEN HGAPLRLPDL GPIGSNGLAN PRDFLAPVAR YEDSRQPLQL 250 260 270 280 290 300 VQKYLGELWA CELDHSPLDV VAWHGNNVPY KYDLRRFNTI GTVSFDHPDP SIFTVLTSPT 310 320 330 340 350 360 SVHGLANIDF VIFPPRWMVA ENTFRPPWFH RNLMNEFMGL IQGAYDAKAG GFVPGGASLH 370 380 390 400 410 420 SCMSAHGPDA ESCDKAIAAD LKPHRIDQTM AFMFETSQVL RPSRAALETP ALQNDYDACW 430 ASLVSTFNPQ RR

References

[1]

"Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial." Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S., Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G., Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M. Genome Biol. 7:RESEARCH90.1-RESEARCH90.14(2006) [PubMed: 17038190] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000438 Genomic DNA. Translation: ABJ11196.1.
RefSeq	YP_791230.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4380294.
GenomeReviews	Gene locus PA14_38510 in contig CP000438_GR.
KEGG	pau:PA14_38510.
NMPDR	fig|208963.3.peg.3613.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEAB

Accession

Primary (citable) accession number: Q02LF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	November 4, 2008
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)