Reviewed, UniProtKB/Swiss-Prot Q02JM4 (ACEK_PSEAB)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name(s)=IDH kinase/phosphatase, IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	PA14_46450

Organism

Pseudomonas aeruginosa (strain UCBPP-PA14) [Complete proteome] [HAMAP]

Taxonomic identifier

208963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP(+))] = ADP + [isocitrate dehydrogenase (NADP(+))] phosphate.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 577	577	Isocitrate dehydrogenase kinase/phosphatase
Regions
	Nucleotide binding	318 – 324	7	ATP By similarity
Sites
	Active site	374	1	By similarity
	Binding site	339	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02JM4-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: DA387F6FC2C1F84B	FASTA	577	66,761
	10 20 30 40 50 60 MVQSAPASEI AALILRGFDD YREQFREITD GARARFEQAQ WQEAQRASTQ RINLYEEKVA 70 80 90 100 110 120 ETVAGLRVGL ADSELLDVER WPIIKSAYIA QIDLRLDDEL AETWFNSIFC GLFSHDNISD 130 140 150 160 170 180 GTMFVHTTRP SLRAHARAPY TRTYRPGGDL RQALEKIFDD YRFDVPYDDR ERDLERIDAL 190 200 210 220 230 240 LHSNLPDWVC KDPDLAIELI GSVFYRNKGA YLVGRLFTPD EQWPLVFPLL HREGHGIQFD 250 260 270 280 290 300 TVITDEAEVS IIFSFTRSYF MVDVPVPAEL VAFLKRLLPG KHLAELYTSI GFYKQGKSEF 310 320 330 340 350 360 YRALINHLAT TDDRFVMAPG VRGMVMSVFT LPGFNTVFKI IKDRFNPSKS VDHATVIQKY 370 380 390 400 410 420 QLVKNHDRVG RLADTQQFAD FRFPVSKFEP ECLAELLEVA PSTVVMEGDV VLIRHCWTER 430 440 450 460 470 480 RMTPLNIYLE NASEAQTREA LNDYGLAIKQ LAAANIFPGD MLLKNFGVTR HGRVVFYDYD 490 500 510 520 530 540 EICYLTEVNF RYIPEPRFPE DEMSSEPWYS VGPNDVFPEE FPRFLFVDLN QRRLFAKLHG 550 560 570 NLYDAKYWQG LQEQIREGKV IDVFPYRRQE TPEQLLG

References

[1]

"Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial." Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S., Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G., Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M. Genome Biol. 7:RESEARCH90.1-RESEARCH90.14(2006) [PubMed: 17038190] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000438 Genomic DNA. Translation: ABJ10571.1.
RefSeq	YP_791862.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4382320.
GenomeReviews	Gene locus PA14_46450 in contig CP000438_GR.
KEGG	pau:PA14_46450.
NMPDR	fig|208963.3.peg.3009.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. IsoCit_DHase_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	Q02JM4. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACEK_PSEAB

Accession

Primary (citable) accession number: Q02JM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	November 14, 2006
Last modified:	July 22, 2008
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)