Reviewed, UniProtKB/Swiss-Prot Q02G92 (RTCA_PSEAB)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	PA14_60670

Organism

Pseudomonas aeruginosa (strain UCBPP-PA14) [Complete proteome] [HAMAP]

Taxonomic identifier

208963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_1000012113
Sites
	Active site	308	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02G92-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: E7277E130981D24F	FASTA	341	36,637
	10 20 30 40 50 60 MRKDLIELDG SEGGGQILRS ALSLSMTSGQ PLRIRNIRGR RSRPGLLRQH LTAVRAAAEI 70 80 90 100 110 120 CAAEVEGAEL GSRELAFRPG AIRAGDYAFA IGSAGSCSLV LQTLLPALLA ANGESRVRIS 130 140 150 160 170 180 GGTHNPLAPP ADFLRDSWLP LLQRMGAEVD LELLRHGFVP AGGGELLARV RPARWRPLQL 190 200 210 220 230 240 EHPGAALRRQ ARALLAGIPG HVGERELERV RQRLGWSDEE RQLEFLAEDQ GPGNALLLRI 250 260 270 280 290 300 DCEHICATFC AFGQAGVSAE RVAEQVATQA IGWMESGCAA DEHLADQLLL PMALAGAGSF 310 320 330 340 TTPRLSAHLQ SNRRVIERFL PVRIGDQALD GGGHRIVITS A

References

[1]

"Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial." Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S., Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G., Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M. Genome Biol. 7:R90.1-R90.14(2006) [PubMed: 17038190] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000438 Genomic DNA. Translation: ABJ13963.1.
RefSeq	YP_793044.1.
3D structure databases
SMR	Q02G92. Positions 5-332.
ModBase	Search...
Protein-protein interaction databases
STRING	Q02G92.
Genome annotation databases
GeneID	4385717.
GenomeReviews	Gene locus PA14_60670 in contig CP000438_GR.
KEGG	pau:PA14_60670.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0430.
HOGENOM	HBG683539.
OMA	EIDGSYG.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR000228. RNA3'_term_phos_cycl-like. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR020723. RNA3'_term_phos_cycl_prd. IPR017770. RNA3'_term_phos_cycl_sub. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_PSEAB

Accession

Primary (citable) accession number: Q02G92

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	February 9, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)