Reviewed, UniProtKB/Swiss-Prot Q02C42 (ALLB_SOLUE)

Names and origin

Protein names

Recommended name:
    Allantoinase
    EC=3.5.2.5
Alternative name(s):
    Allantoin-utilizing enzyme

Gene names

Name:	allB
Ordered Locus Names:	Acid_0362

Organism

Solibacter usitatus (strain Ellin6076) [Complete proteome] [HAMAP]

Taxonomic identifier

234267 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Solibacteres › Solibacterales › Solibacteraceae › Candidatus Solibacter

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring By similarity.
Catalytic activity	(S)-allantoin + H2O = allantoate. HAMAP MF_01645
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Nitrogen metabolism; (S)-allantoin degradation; allantoate from (S)-allantoin: step 1/1. HAMAP MF_01645
Subunit structure	Homotetramer By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two zinc ions By similarity.
Sequence similarities	Belongs to the DHOase family. Allantoinase subfamily.

Ontologies

Keywords
Biological process	Purine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	allantoin catabolic process Inferred from electronic annotation. Source: HAMAP purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	allantoinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Allantoinase HAMAP MF_01645		PRO_0000317686
Sites
	Metal binding	58	1	Zinc 1 By similarity
	Metal binding	60	1	Zinc 1 By similarity
	Metal binding	145	1	Zinc 1; via carbamate group By similarity
	Metal binding	145	1	Zinc 2; via carbamate group By similarity
	Metal binding	181	1	Zinc 2 By similarity
	Metal binding	237	1	Zinc 2 By similarity
	Metal binding	310	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	145	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02C42-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 1ECA27B484510AA9	FASTA	457	48,807
	10 20 30 40 50 60 MSECVVRGGQ VVQAGGVVSA DVAIEDGRIS AIGPELPGAK REINARGLTV FPGVIDDHLH 70 80 90 100 110 120 FNEPGRTEWE GAATGSRALA AGGGTAFFDM PLNSTPCTVD RAAFAGKRAA LERASIADFA 130 140 150 160 170 180 LWGGIVPGNL SELAPLAESG VIGFKAFLSD SGLPEFPRAD DLTLYEGMRE AARLGLPVAV 190 200 210 220 230 240 HAESEEITSR LTRRARDAGR TSARDYLDSR PVIAEVEAIH RAALLAREAG CKLHIVHISS 250 260 270 280 290 300 GRGVAAALEA RTMGTDIAIE TCAHYLFFTE DDLLRLGAIA KCAPPLRDRQ EFDRLWAHVL 310 320 330 340 350 360 GGIVDVVASD HSPAPPAMKT GDDFFAIWGG IAGVQSTLAV LLEAGHFQRG LRLERIAELT 370 380 390 400 410 420 AGYPARRFAL HNKGSIAVGN DADLTLVDMD GQENLEPESL WQKHPVNPYT GNSFRGSIRR 430 440 450 TMLRGTTIFN HDEITVKSGG QLLRPKANTY ATSGIHP

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000473 Genomic DNA. Translation: ABJ81374.1.
RefSeq	YP_821659.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q02C42.
Genome annotation databases
GeneID	4431577.
GenomeReviews	Gene locus Acid_0362 in contig CP000473_GR.
KEGG	sus:Acid_0362.
NMPDR	fig|234267.9.peg.343.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FEAWGGI.
Family and domain databases
HAMAP	MF_01645. [Tree]
InterPro	IPR017593. Allantoinase. IPR006680. Amidohydro_1. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03178. allantoinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALLB_SOLUE

Accession

Primary (citable) accession number: Q02C42

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 14, 2006
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)