Q02A20 (KATG_SOLUE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Acid_1112

Organism

Solibacter usitatus (strain Ellin6076) [Complete proteome] [HAMAP]

Taxonomic identifier

234267 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Solibacteres › Solibacterales › Solibacteraceae › Candidatus Solibacter

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	Catalase-peroxidase HAMAP MF_01961		PRO_0000354936
Sites
	Active site	106	1	Proton acceptor By similarity
	Metal binding	268	1	Iron (heme axial ligand) By similarity
	Site	102	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	105 ↔ 227		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-253) By similarity
	Cross-link	227 ↔ 253		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-105) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02A20 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 15497A51D56B3C8B	FASTA	743	81,479
	10 20 30 40 50 60 MEKQSNDAAV AGAPNDHGAA KCPVAHTAHG RRNRDWWPDA LDISTLHRNS SLSDPMGSGF 70 80 90 100 110 120 DYAMEFESLD LQAVIKDLHA LMTDSQDWWP ADFGHYGGLM IRMAWHSAGT YRITDGRGGA 130 140 150 160 170 180 GAGQQRFAPL NSWPDNANLD KARRLLWPVK QKYGDKISWA DLMVLAGNVA LESMGFKTFG 190 200 210 220 230 240 FAGGRSDVWE PEELYWGPEG TWLGDERYSG ERQLSEPLGA VQMGLIYVNP EGPNGNPDPV 250 260 270 280 290 300 AAAKDIRETF FRMAMNDEET VALIAGGHTF GKTHGAGDPS LVGPDPESGA LEDQGLGWKS 310 320 330 340 350 360 KFGTGFGADA ITGGPEVTWS QTPTQWSNSF FKNLFENEWE LTNSPAGAKQ WRAKNGQATI 370 380 390 400 410 420 PDPFDKSKKR VPTMLTTDLS LRFDPAYEKI SRRFYENPDQ FADAFARAWF KLTHRDMGPI 430 440 450 460 470 480 ARYLGPLVPK ETLLWQDPIP PVDHPLIDEN DASALKAKIL ASGLSTSDLV STAWASASTF 490 500 510 520 530 540 RGSDKRGGAN GARIRLAPQK DWEVNQPKQL AKVLEKLETI GKEFGKKVSL ADLIVLGGDA 550 560 570 580 590 600 AIEKAAKDAG VEVKIPFTPG RMDASQEQTD VASFAPLEPK ADGFRNYIGR KTQFLQPEEA 610 620 630 640 650 660 LVDRAQLLRL TGPEMTVLVG GLRVLGANTA GSKHGVFTAT PGVLTNDFFV NLLDMRTHWQ 670 680 690 700 710 720 PAGGEEGVYE GRDRKTNALK WTGTRVDLIF GSHSQLRAFA EVYAWAGSKE KFVKDFAAAW 730 740 NKVMNLDRYD VARTGETKKA AVV

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ellin6076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000473 Genomic DNA. Translation: ABJ82106.1.
RefSeq	YP_822391.1. NC_008536.1.
3D structure databases
ProteinModelPortal	Q02A20.
SMR	Q02A20. Positions 29-732.
ModBase	Search...
Protein-protein interaction databases
STRING	Q02A20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4430727.
GenomeReviews	Gene locus Acid_1112 in contig CP000473_GR.
KEGG	sus:Acid_1112.
NMPDR	fig|234267.9.peg.1050.
PATRIC	32004345. VBICanSol30224_1162.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	GPEVIWT.
PhylomeDB	Q02A20.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_SOLUE

Accession

Primary (citable) accession number: Q02A20

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program