Q029M3 (Q029M3_SOLUE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191
EC=1.17.1.2 HAMAP MF_00191

Gene names

Name:	ispH HAMAP MF_00191
Ordered Locus Names:	Acid_1259

Organism

Solibacter usitatus (strain Ellin6076) [Complete proteome] [HAMAP]

Taxonomic identifier

234267 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Solibacteres › Solibacterales › Solibacteraceae › Candidatus Solibacter

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191 SAAS SAAS003451
Catalytic activity	Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 SAAS SAAS003451 Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 SAAS SAAS003451
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 SAAS SAAS003451 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191 SAAS SAAS003451
Sequence similarities	Belongs to the IspH family. HAMAP MF_00191

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00191 SAAS SAAS003451
Ligand	3Fe-4S HAMAP MF_00191 SAAS SAAS003451 Iron Iron-sulfur Metal-binding NADP HAMAP MF_00191 SAAS SAAS003451
Molecular function	Oxidoreductase HAMAP MF_00191 SAAS SAAS003451 EMBL ABJ82253.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q029M3 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 4CDD91945D5B8DAB	FASTA	323	35,767
	10 20 30 40 50 60 MPLKPFAPNF GAEKKIFLLK PRGFCAGVVR AIDVVKIALD LYGPPVYVRK EIVHNKHVVE 70 80 90 100 110 120 ELREAGAVFV EELDEVPSGA RAIFSAHGVS PAVRAQAKER NLEIIDATCP LVTKVHLEAV 130 140 150 160 170 180 RFARDGFSIV LIGHKDHDEV IGTLGEVPDR SYLVETTDDV DNLVLPDPTR VRYLTQTTLS 190 200 210 220 230 240 LDETRDIVDR LKQRFPMIEG PHSQDICYAT ENRQMAVKAI STNVDLLLVV GSENSSNSKR 250 260 270 280 290 300 LVEVGDNFGV RSHLVNDRGD VDASWLDGVK NVGVTAGASA PEHLVQELIE FLREWGFEGL 310 320 EEIELVDEDV RFSLPAELTI SIQ

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000473 Genomic DNA. Translation: ABJ82253.1.
RefSeq	YP_822538.1. NC_008536.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q029M3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4430579.
GenomeReviews	Gene locus Acid_1259 in contig CP000473_GR.
KEGG	sus:Acid_1259.
NMPDR	fig|234267.9.peg.1200.
PATRIC	32004665. VBICanSol30224_1321.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	AMAPECD.
PhylomeDB	Q029M3.
ProtClustDB	CLSK774625.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q029M3_SOLUE

Accession

Primary (citable) accession number: Q029M3

Entry history

Integrated into UniProtKB/TrEMBL:	November 14, 2006
Last sequence update:	November 14, 2006
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)