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Protein

Ketohexokinase

Gene

Khk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.By similarity

Catalytic activityi

ATP + D-fructose = ADP + D-fructose 1-phosphate.

Enzyme regulationi

Requires potassium. Inhibition by ADP.

Pathwayi: fructose metabolism

This protein is involved in the pathway fructose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway fructose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15D-fructoseBy similarity1
Binding sitei41D-fructose; via amide nitrogenBy similarity1
Binding sitei42D-fructoseBy similarity1
Binding sitei45D-fructoseBy similarity1
Binding sitei108ATPBy similarity1
Binding sitei258D-fructoseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi255 – 258ATPBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ketohexokinase activity Source: RGD

GO - Biological processi

  • carbohydrate catabolic process Source: RGD
  • fructose metabolic process Source: RGD
  • response to fructose Source: RGD
  • response to glucose Source: RGD
  • response to insulin Source: RGD
  • response to sucrose Source: RGD
  • response to zinc ion Source: RGD

Keywordsi

Molecular functionKinase, Transferase
Biological processCarbohydrate metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-70350. Fructose catabolism.
SABIO-RKiQ02974.
UniPathwayiUPA00202.

Names & Taxonomyi

Protein namesi
Recommended name:
KetohexokinaseImported (EC:2.7.1.3)
Alternative name(s):
Hepatic fructokinase
Gene namesi
Name:KhkImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi2966. Khk.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000800911 – 298KetohexokinaseAdd BLAST298

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PRIDEiQ02974.

Expressioni

Tissue specificityi

Isoform C is most abundant in liver, kidney, gut, spleen and pancreas. Low levels of isoform A found in adrenal, spleen and brain.1 Publication

Gene expression databases

BgeeiENSRNOG00000008047.
GenevisibleiQ02974. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

MINTiMINT-4578661.

Structurei

3D structure databases

ProteinModelPortaliQ02974.
SMRiQ02974.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000007458.
HOGENOMiHOG000212926.
HOVERGENiHBG003335.
InParanoidiQ02974.
KOiK00846.
OMAiCNKDYPQ.
OrthoDBiEOG091G0EV7.
PhylomeDBiQ02974.
TreeFamiTF323942.

Family and domain databases

CDDicd01939. Ketohexokinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
InterProiView protein in InterPro
IPR034093. KHK.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
PfamiView protein in Pfam
PF00294. PfkB. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform C1 Publication (identifier: Q02974-1) [UniParc]FASTAAdd to basket
Also known as: Central, hepatic/renal/intestinal1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEKQILCVG LVVLDIINVV DKYPEEDTDR RCLSQRWQRG GNASNSCTVL
60 70 80 90 100
SLLGARCAFM GSLAHGHVAD FLVADFRRRG VDVSQVAWQS QGDTPCSCCI
110 120 130 140 150
VNNSNGSRTI ILYDTNLPDV SAKDFEKVDL TRFKWIHIEG RNASEQVKML
160 170 180 190 200
QRIEQYNATQ PLQQKVRVSV EIEKPREELF QLFGYGEVVF VSKDVAKHLG
210 220 230 240 250
FRSAGEALKG LYSRVKKGAT LICAWAEEGA DALGPDGQLL HSDAFPPPRV
260 270 280 290
VDTLGAGDTF NASVIFSLSK GNSMQEALRF GCQVAGKKCG LQGFDGIV
Length:298
Mass (Da):32,749
Last modified:July 1, 1993 - v1
Checksum:iF85C787FF047DF70
GO
Isoform A1 Publication (identifier: Q02974-2) [UniParc]FASTAAdd to basket
Also known as: Peripheral1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     72-114: LVADFRRRGV...NGSRTIILYD → VLDDLRRHSV...SGSRTILHAY

Show »
Length:298
Mass (Da):32,758
Checksum:i35884AE0EBF90754
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232A → T in CAA70517 (PubMed:9799106).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00467072 – 114LVADF…IILYD → VLDDLRRHSVDLRYAVLQTE GSIPTSTVIVNEASGSRTIL HAY in isoform A. CuratedAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63658 mRNA. Translation: CAA45198.1.
Y09337 Genomic DNA. Translation: CAA70517.1.
Y09338 Genomic DNA. Translation: CAA70518.1.
Y09338 Genomic DNA. Translation: CAA70519.1.
Y09339 Genomic DNA. Translation: CAA70520.1.
BC078752 mRNA. Translation: AAH78752.1.
PIRiS32426.
RefSeqiNP_114061.1. NM_031855.3. [Q02974-1]
UniGeneiRn.33324.

Genome annotation databases

EnsembliENSRNOT00000043572; ENSRNOP00000046296; ENSRNOG00000008047. [Q02974-1]
GeneIDi25659.
KEGGirno:25659.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKHK_RAT
AccessioniPrimary (citable) accession number: Q02974
Secondary accession number(s): P97550, P97551
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: August 30, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families