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Protein

Ketohexokinase

Gene

Khk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.By similarity

Catalytic activityi

ATP + D-fructose = ADP + D-fructose 1-phosphate.

Enzyme regulationi

Requires potassium. Inhibition by ADP.

Pathwayi: fructose metabolism

This protein is involved in the pathway fructose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway fructose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151D-fructoseBy similarity
Binding sitei41 – 411D-fructose; via amide nitrogenBy similarity
Binding sitei42 – 421D-fructoseBy similarity
Binding sitei45 – 451D-fructoseBy similarity
Binding sitei108 – 1081ATPBy similarity
Binding sitei258 – 2581D-fructoseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi255 – 2584ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ketohexokinase activity Source: RGD

GO - Biological processi

  • carbohydrate catabolic process Source: RGD
  • fructose metabolic process Source: RGD
  • response to fructose Source: RGD
  • response to glucose Source: RGD
  • response to insulin Source: RGD
  • response to sucrose Source: RGD
  • response to zinc ion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-70350. Fructose catabolism.
SABIO-RKQ02974.
UniPathwayiUPA00202.

Names & Taxonomyi

Protein namesi
Recommended name:
KetohexokinaseImported (EC:2.7.1.3)
Alternative name(s):
Hepatic fructokinase
Gene namesi
Name:KhkImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi2966. Khk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298KetohexokinasePRO_0000080091Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PRIDEiQ02974.

Expressioni

Tissue specificityi

Isoform C is most abundant in liver, kidney, gut, spleen and pancreas. Low levels of isoform A found in adrenal, spleen and brain.1 Publication

Gene expression databases

ExpressionAtlasiQ02974. baseline and differential.
GenevisibleiQ02974. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

MINTiMINT-4578661.

Structurei

3D structure databases

ProteinModelPortaliQ02974.
SMRiQ02974. Positions 3-298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000007458.
HOGENOMiHOG000212926.
HOVERGENiHBG003335.
InParanoidiQ02974.
KOiK00846.
PhylomeDBiQ02974.
TreeFamiTF323942.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform C1 Publication (identifier: Q02974-1) [UniParc]FASTAAdd to basket

Also known as: Central, hepatic/renal/intestinal1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEKQILCVG LVVLDIINVV DKYPEEDTDR RCLSQRWQRG GNASNSCTVL
60 70 80 90 100
SLLGARCAFM GSLAHGHVAD FLVADFRRRG VDVSQVAWQS QGDTPCSCCI
110 120 130 140 150
VNNSNGSRTI ILYDTNLPDV SAKDFEKVDL TRFKWIHIEG RNASEQVKML
160 170 180 190 200
QRIEQYNATQ PLQQKVRVSV EIEKPREELF QLFGYGEVVF VSKDVAKHLG
210 220 230 240 250
FRSAGEALKG LYSRVKKGAT LICAWAEEGA DALGPDGQLL HSDAFPPPRV
260 270 280 290
VDTLGAGDTF NASVIFSLSK GNSMQEALRF GCQVAGKKCG LQGFDGIV
Length:298
Mass (Da):32,749
Last modified:July 1, 1993 - v1
Checksum:iF85C787FF047DF70
GO
Isoform A1 Publication (identifier: Q02974-2) [UniParc]FASTAAdd to basket

Also known as: Peripheral1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     72-114: LVADFRRRGV...NGSRTIILYD → VLDDLRRHSV...SGSRTILHAY

Show »
Length:298
Mass (Da):32,758
Checksum:i35884AE0EBF90754
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti232 – 2321A → T in CAA70517 (PubMed:9799106).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei72 – 11443LVADF…IILYD → VLDDLRRHSVDLRYAVLQTE GSIPTSTVIVNEASGSRTIL HAY in isoform A. CuratedVSP_004670Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63658 mRNA. Translation: CAA45198.1.
Y09337 Genomic DNA. Translation: CAA70517.1.
Y09338 Genomic DNA. Translation: CAA70518.1.
Y09338 Genomic DNA. Translation: CAA70519.1.
Y09339 Genomic DNA. Translation: CAA70520.1.
BC078752 mRNA. Translation: AAH78752.1.
PIRiS32426.
RefSeqiNP_114061.1. NM_031855.3. [Q02974-1]
UniGeneiRn.33324.

Genome annotation databases

EnsembliENSRNOT00000043572; ENSRNOP00000046296; ENSRNOG00000008047. [Q02974-1]
GeneIDi25659.
KEGGirno:25659.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63658 mRNA. Translation: CAA45198.1.
Y09337 Genomic DNA. Translation: CAA70517.1.
Y09338 Genomic DNA. Translation: CAA70518.1.
Y09338 Genomic DNA. Translation: CAA70519.1.
Y09339 Genomic DNA. Translation: CAA70520.1.
BC078752 mRNA. Translation: AAH78752.1.
PIRiS32426.
RefSeqiNP_114061.1. NM_031855.3. [Q02974-1]
UniGeneiRn.33324.

3D structure databases

ProteinModelPortaliQ02974.
SMRiQ02974. Positions 3-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4578661.

Proteomic databases

PRIDEiQ02974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000043572; ENSRNOP00000046296; ENSRNOG00000008047. [Q02974-1]
GeneIDi25659.
KEGGirno:25659.

Organism-specific databases

CTDi3795.
RGDi2966. Khk.

Phylogenomic databases

GeneTreeiENSGT00390000007458.
HOGENOMiHOG000212926.
HOVERGENiHBG003335.
InParanoidiQ02974.
KOiK00846.
PhylomeDBiQ02974.
TreeFamiTF323942.

Enzyme and pathway databases

UniPathwayiUPA00202.
ReactomeiR-RNO-70350. Fructose catabolism.
SABIO-RKQ02974.

Miscellaneous databases

NextBioi607557.
PROiQ02974.

Gene expression databases

ExpressionAtlasiQ02974. baseline and differential.
GenevisibleiQ02974. RN.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of rat liver ketohexokinase in yeast results in fructose intolerance."
    Donaldson I.A., Doyle T.C., Matas N.
    Biochem. J. 291:179-186(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Structure and alternative splicing of the ketohexokinase gene."
    Hayward B.E., Bonthron D.T.
    Eur. J. Biochem. 257:85-91(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
    Tissue: Kidney.

Entry informationi

Entry nameiKHK_RAT
AccessioniPrimary (citable) accession number: Q02974
Secondary accession number(s): P97550, P97551
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 11, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.