Reviewed, UniProtKB/Swiss-Prot Q02974 (KHK_RAT)

Names and origin

Protein names

Recommended name:
    Ketohexokinase
    EC=2.7.1.3
Alternative name(s):
    Hepatic fructokinase

Gene names

Name:

Khk

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose = ADP + D-fructose 1-phosphate.
Enzyme regulation	Requires potassium. Inhibition by ADP.
Pathway	Carbohydrate metabolism; fructose metabolism.
Subunit structure	Homodimer By similarity.
Tissue specificity	Most abundant in the liver.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate catabolic process Ref.2 Traceable author statement. Source: RGD fructose metabolic process Ref.1 Inferred from direct assay. Source: RGD response to fructose stimulus Inferred from expression pattern. Source: RGD response to glucose stimulus Inferred from direct assay. Source: RGD response to insulin stimulus Inferred from direct assay. Source: RGD response to sucrose stimulus Inferred from direct assay. Source: RGD response to zinc ion Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ketohexokinase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform C (identifier: Q02974-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: Q02974-2)

The sequence of this isoform differs from the canonical sequence as follows:
     72-114: LVADFRRRGV...NGSRTIILYD → VLDDLRRHSV...SGSRTILHAY

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Ketohexokinase		PRO_0000080091
Regions
	Nucleotide binding	255 – 258	4	ATP By similarity
Sites
	Binding site	15	1	D-fructose By similarity
	Binding site	41	1	D-fructose; via amide nitrogen By similarity
	Binding site	42	1	D-fructose By similarity
	Binding site	45	1	D-fructose By similarity
	Binding site	108	1	ATP By similarity
	Binding site	258	1	D-fructose By similarity
Amino acid modifications
	Modified residue	169	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	72 – 114	43	LVADF…IILYD → VLDDLRRHSVDLRYAVLQTE GSIPTSTVIVNEASGSRTIL HAY in isoform A.		VSP_004670
Experimental info
	Sequence conflict	232	1	A → T in CAA70517. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform C [UniParc]. Last modified July 1, 1993. Version 1. Checksum: F85C787FF047DF70	FASTA	298	32,749
	10 20 30 40 50 60 MEEKQILCVG LVVLDIINVV DKYPEEDTDR RCLSQRWQRG GNASNSCTVL SLLGARCAFM 70 80 90 100 110 120 GSLAHGHVAD FLVADFRRRG VDVSQVAWQS QGDTPCSCCI VNNSNGSRTI ILYDTNLPDV 130 140 150 160 170 180 SAKDFEKVDL TRFKWIHIEG RNASEQVKML QRIEQYNATQ PLQQKVRVSV EIEKPREELF 190 200 210 220 230 240 QLFGYGEVVF VSKDVAKHLG FRSAGEALKG LYSRVKKGAT LICAWAEEGA DALGPDGQLL 250 260 270 280 290 HSDAFPPPRV VDTLGAGDTF NASVIFSLSK GNSMQEALRF GCQVAGKKCG LQGFDGIV
	Isoform A. Checksum: 35884AE0EBF90754	FASTA	298	32,758
	10 20 30 40 50 60 MEEKQILCVG LVVLDIINVV DKYPEEDTDR RCLSQRWQRG GNASNSCTVL SLLGARCAFM 70 80 90 100 110 120 GSLAHGHVAD FVLDDLRRHS VDLRYAVLQT EGSIPTSTVI VNEASGSRTI LHAYTNLPDV 130 140 150 160 170 180 SAKDFEKVDL TRFKWIHIEG RNASEQVKML QRIEQYNATQ PLQQKVRVSV EIEKPREELF 190 200 210 220 230 240 QLFGYGEVVF VSKDVAKHLG FRSAGEALKG LYSRVKKGAT LICAWAEEGA DALGPDGQLL 250 260 270 280 290 HSDAFPPPRV VDTLGAGDTF NASVIFSLSK GNSMQEALRF GCQVAGKKCG LQGFDGIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of rat liver ketohexokinase in yeast results in fructose intolerance." Donaldson I.A., Doyle T.C., Matas N. Biochem. J. 291:179-186(1993) [PubMed: 8471037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Structure and alternative splicing of the ketohexokinase gene." Hayward B.E., Bonthron D.T. Eur. J. Biochem. 257:85-91(1998) [PubMed: 9799106] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63658 mRNA. Translation: CAA45198.1. Y09337 Genomic DNA. Translation: CAA70517.1. Y09338 Genomic DNA. Translation: CAA70518.1. Y09338 Genomic DNA. Translation: CAA70519.1. Y09339 Genomic DNA. Translation: CAA70520.1. BC078752 mRNA. Translation: AAH78752.1.
IPI	IPI00211989. IPI00231910.
PIR	S32426.
RefSeq	NP_114061.1.
UniGene	Rn.33324
3D structure databases
SMR	Q02974. Positions 3-298.
ModBase	Search...
Protein-protein interaction databases
STRING	Q02974.
Proteomic databases
PRIDE	Q02974.
Genome annotation databases
Ensembl	ENSRNOT00000010867; ENSRNOP00000010867; ENSRNOG00000008047; Rattus norvegicus. [Genome view]
GeneID	25659.
KEGG	rno:25659.
UCSC	NM_031855. rat.
Organism-specific databases
CTD	25659.
RGD	2966. Khk.
Phylogenomic databases
eggNOG	roNOG16678.
HOVERGEN	Q02974.
InParanoid	Q02974.
Enzyme and pathway databases
BRENDA	2.7.1.3. 248.
Gene expression databases
ArrayExpress	Q02974.
Genevestigator	Q02974.
GermOnline	ENSRNOG00000008047. Rattus norvegicus.
Family and domain databases
InterPro	IPR011611. Carb/pur_kinase. [Graphical view]
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
PROSITE	PS00583. PFKB_KINASES_1. False negative. PS00584. PFKB_KINASES_2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	607557.

Entry information

Entry name

KHK_RAT

Accession

Primary (citable) accession number: Q02974
Secondary accession number(s): P97550, P97551

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	January 19, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)