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Protein

Cinnamyl alcohol dehydrogenase 8

Gene

CAD8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in lignin biosynthesis. Catalyzes the final step specific for the production of lignin monomers. Catalyzes the NADPH-dependent reduction of coniferaldehyde, 5-hydroxyconiferaldehyde, sinapaldehyde, 4-coumaraldehyde and caffeyl aldehyde to their respective alcohols.1 Publication

Catalytic activityi

Cinnamyl alcohol + NADP+ = cinnamaldehyde + NADPH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Kineticsi

  1. KM=302 µM for 4-coumaraldehyde (at pH 6.25 and 42 degrees Celsius)1 Publication
  2. KM=683 µM for caffeyl aldehyde (at pH 6.0 and 35 degrees Celsius)1 Publication
  3. KM=141 µM for coniferaldehyde (at pH 6.25 and 42 degrees Celsius)1 Publication
  4. KM=457 µM for 5-hydroxyconiferaldehyde (at pH 6.25 and 42 degrees Celsius)1 Publication
  5. KM=898 µM for sinapaldehyde (at pH 6.25-6.5 and 25 degrees Celsius)1 Publication
  1. Vmax=20.4 pmol/sec/µg enzyme with 4-coumaraldehyde as substrate (at pH 6.25 and 42 degrees Celsius)1 Publication
  2. Vmax=7.0 pmol/sec/µg enzyme with caffeyl aldehyde as substrate (at pH 6.0 and 35 degrees Celsius)1 Publication
  3. Vmax=5.6 pmol/sec/µg enzyme with coniferaldehyde as substrate (at pH 6.25 and 42 degrees Celsius)1 Publication
  4. Vmax=10.4 pmol/sec/µg enzyme with 5-hydroxyconiferaldehyde as substrate (at pH 6.25 and 42 degrees Celsius)1 Publication
  5. Vmax=28.9 pmol/sec/µg enzyme with sinapaldehyde as substrate (at pH 6.25-6.5 and 25 degrees Celsius)1 Publication

Pathwayi: phenylpropanoid biosynthesis

This protein is involved in the pathway phenylpropanoid biosynthesis, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenylpropanoid biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi46Zinc 1; catalyticBy similarity1
Binding sitei48NADPBy similarity1
Metal bindingi68Zinc 1; catalyticBy similarity1
Metal bindingi69Zinc 1; catalyticBy similarity1
Metal bindingi99Zinc 2By similarity1
Metal bindingi102Zinc 2By similarity1
Metal bindingi105Zinc 2By similarity1
Metal bindingi113Zinc 2By similarity1
Metal bindingi162Zinc 1; catalyticBy similarity1
Binding sitei166NADPBy similarity1
Binding sitei250NADPBy similarity1
Binding sitei274NADP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi187 – 192NADPBy similarity6
Nucleotide bindingi210 – 215NADPBy similarity6
Nucleotide bindingi297 – 299NADPBy similarity3

GO - Molecular functioni

  • aryl-alcohol dehydrogenase (NADP+) activity Source: TAIR
  • cinnamyl-alcohol dehydrogenase activity Source: UniProtKB
  • sinapyl alcohol dehydrogenase activity Source: UniProtKB-EC
  • zinc ion binding Source: InterPro

GO - Biological processi

  • lignin biosynthetic process Source: UniProtKB
  • response to bacterium Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processLignin biosynthesis
LigandMetal-binding, NADP, Zinc

Enzyme and pathway databases

BioCyciARA:AT4G37990-MONOMER
SABIO-RKQ02972
UniPathwayiUPA00711

Names & Taxonomyi

Protein namesi
Recommended name:
Cinnamyl alcohol dehydrogenase 8 (EC:1.1.1.195)
Short name:
AtCAD8
Alternative name(s):
NAD-dependent mannitol dehydrogenase 2
Gene namesi
Name:CAD8
Synonyms:BAD, CAD5, CADB2, ELI3-2
Ordered Locus Names:At4g37990
ORF Names:F20D10.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G37990
TAIRilocus:2005528 AT4G37990

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001608091 – 359Cinnamyl alcohol dehydrogenase 8Add BLAST359

Proteomic databases

PaxDbiQ02972
PRIDEiQ02972

Expressioni

Tissue specificityi

Expressed in the differentiation and elongation zones of primary and lateral roots. Expressed in the hypocotyl, cotyledon veins, vasculature of the first rosette leaves, hydathodes and trichomes. In stems, expressed in the vascular cambium and developing xylem tissues. Expressed in the style, anthers, stamen filaments, stigmatic regions in flowers, and abscission and style regions of siliques.2 Publications

Gene expression databases

ExpressionAtlasiQ02972 baseline and differential
GenevisibleiQ02972 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G37990.1

Structurei

3D structure databases

ProteinModelPortaliQ02972
SMRiQ02972
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0023 Eukaryota
COG1064 LUCA
HOGENOMiHOG000294667
InParanoidiQ02972
KOiK00083
OMAiYRFSIDM
OrthoDBiEOG09360F7A
PhylomeDBiQ02972

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequencei

Sequence statusi: Complete.

Q02972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKVLQKEAF GLAAKDNSGV LSPFSFTRRE TGEKDVRFKV LFCGICHSDL
60 70 80 90 100
HMVKNEWGMS TYPLVPGHEI VGVVTEVGAK VTKFKTGEKV GVGCLVSSCG
110 120 130 140 150
SCDSCTEGME NYCPKSIQTY GFPYYDNTIT YGGYSDHMVC EEGFVIRIPD
160 170 180 190 200
NLPLDAAAPL LCAGITVYSP MKYHGLDKPG MHIGVVGLGG LGHVGVKFAK
210 220 230 240 250
AMGTKVTVIS TSEKKRDEAI NRLGADAFLV SRDPKQIKDA MGTMDGIIDT
260 270 280 290 300
VSATHSLLPL LGLLKHKGKL VMVGAPEKPL ELPVMPLIFE RKMVMGSMIG
310 320 330 340 350
GIKETQEMID MAGKHNITAD IELISADYVN TAMERLEKAD VRYRFVIDVA

NTLKPNPNL
Length:359
Mass (Da):38,942
Last modified:November 1, 1995 - v1
Checksum:iB691F9B8AD4842A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67815 mRNA Translation: CAA48026.1
AY302080 mRNA Translation: AAP59433.1
AL035538 Genomic DNA Translation: CAB37539.1
AL161592 Genomic DNA Translation: CAB80464.1
CP002687 Genomic DNA Translation: AEE86861.1
AF361859 mRNA Translation: AAK32871.1
AY129478 mRNA Translation: AAM91064.1
PIRiS28043
RefSeqiNP_195512.1, NM_119960.3
UniGeneiAt.41

Genome annotation databases

EnsemblPlantsiAT4G37990.1; AT4G37990.1; AT4G37990
GeneIDi829955
GrameneiAT4G37990.1; AT4G37990.1; AT4G37990
KEGGiath:AT4G37990

Similar proteinsi

Entry informationi

Entry nameiCADH8_ARATH
AccessioniPrimary (citable) accession number: Q02972
Secondary accession number(s): Q53ZN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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