Q02969 (PEX25_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal membrane protein PEX25

Alternative name(s):
Peroxin-25

Gene names

Name:	PEX25
Ordered Locus Names:	YPL112C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for regulation of peroxisome size and maintenance. Has a role in the import of peroxisomal matrix proteins. Imports RHO1 into the peroxisome. Also promotes peroxisome division and biogenesis. Ref.4 Ref.5 Ref.6 Ref.9 Ref.13
Subunit structure	Homooligomer. Interacts with PEX27 and PEX34. Ref.4 Ref.6 Ref.13
Subcellular location	Peroxisome membrane; Single-pass membrane protein Ref.4 Ref.5 Ref.7 Ref.9.
Miscellaneous	Present with 2420 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Peroxisome biogenesis
Cellular component	Membrane Peroxisome
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	peroxisome fission Inferred from direct assay PubMed 20826455. Source: UniProtKB protein import into peroxisome matrix Inferred from mutant phenotype Ref.5. Source: SGD regulation of peroxisome size Inferred from direct assay PubMed 20826455. Source: UniProtKB
Cellular_component	integral to peroxisomal membrane Inferred from electronic annotation. Source: InterPro peroxisomal membrane Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Peroxisomal membrane protein PEX25		PRO_0000270568
Regions
	Topological domain	1 – 366	366	Cytoplasmic Potential
	Transmembrane	367 – 383	17	Helical; Potential
	Topological domain	384 – 394	11	Lumenal Potential
Amino acid modifications
	Modified residue	58	1	Phosphoserine Ref.10
	Modified residue	63	1	Phosphoserine Ref.10
	Modified residue	289	1	Phosphoserine Ref.12
	Modified residue	304	1	Phosphoserine Ref.10 Ref.11 Ref.12
Experimental info
	Sequence conflict	8	1	D → N in AAT92840. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q02969 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A453AF4B5E41DB68	FASTA	394	44,911
	10 20 30 40 50 60 MSQFGTTDIV SGSETPPYSG ASYQDAQDDN THPHSSDAGA EKFSAGSGSE SHTESSRSDD 70 80 90 100 110 120 EDSQAKTKMV DNITILKYIL DSLSGRDKLA KIIKYALDIL KLFIEKSKRN LTVLDPSVLT 130 140 150 160 170 180 YYTKILKNLT VKVALRHPIT VIKVLLLSLL RNFDKKIDFI SQQLSTFRYI LRFGGTPFRV 190 200 210 220 230 240 CSFLGKFNKT RKCNFQIDQI KKIWFNEASL REFLDLYYGI FDELDLLYKL KIWTNKSFYS 250 260 270 280 290 300 FVSRQESLAW QYDILLSLKD HWLNLQSLQK RQLELEVQLK VQNNALLLSP ILMHQAHKDD 310 320 330 340 350 360 GSQSPIRKQL LNDLNVNNDA EVLIHKQLKA IKDEKTLVYL DIARLSFDCM ANTSDILNLK 370 380 390 TPKGTYAVLS LGSGLTGLVK LWITTKRSLC SSKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Conserved function of pex11p and the novel pex25p and pex27p in peroxisome biogenesis." Rottensteiner H., Stein K., Sonnenhol E., Erdmann R. Mol. Biol. Cell 14:4316-4328(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 56-70 AND 337-354, FUNCTION, SUBUNIT, INTERACTION WITH PEX27, SUBCELLULAR LOCATION. Strain: ATCC 201389 / BY4742.
[5]	"Transcriptome profiling to identify genes involved in peroxisome assembly and function." Smith J.J., Marelli M., Christmas R.H., Vizeacoumar F.J., Dilworth D.J., Ideker T., Galitski T., Dimitrov K., Rachubinski R.A., Aitchison J.D. J. Cell Biol. 158:259-271(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Pex11-related proteins in peroxisome dynamics: a role for the novel peroxin Pex27p in controlling peroxisome size and number in Saccharomyces cerevisiae." Tam Y.Y.C., Torres-Guzman J.C., Vizeacoumar F.J., Smith J.J., Marelli M., Aitchison J.D., Rachubinski R.A. Mol. Biol. Cell 14:4089-4102(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH PEX27.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane." Marelli M., Smith J.J., Jung S., Yi E., Nesvizhskii A.I., Christmas R.H., Saleem R.A., Tam Y.Y.C., Fagarasanu A., Goodlett D.R., Aebersold R., Rachubinski R.A., Aitchison J.D. J. Cell Biol. 167:1099-1112(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58; SER-63 AND SER-304, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289 AND SER-304, MASS SPECTROMETRY.
[13]	"The peroxin Pex34p functions with the Pex11 family of peroxisomal divisional proteins to regulate the peroxisome population in yeast." Tower R.J., Fagarasanu A., Aitchison J.D., Rachubinski R.A. Mol. Biol. Cell 22:1727-1738(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PEX34.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U43503 Genomic DNA. Translation: AAB68249.1. AY692821 Genomic DNA. Translation: AAT92840.1. BK006949 Genomic DNA. Translation: DAA11321.1.
PIR	S62009.
RefSeq	NP_015213.1. NM_001183926.1.
3D structure databases
ProteinModelPortal	Q02969.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5292N.
IntAct	Q02969. 5 interactions.
MINT	MINT-504153.
STRING	4932.YPL112C.
Proteomic databases
PaxDb	Q02969.
PeptideAtlas	Q02969.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL112C; YPL112C; YPL112C.
GeneID	855991.
KEGG	sce:YPL112C.
Organism-specific databases
CYGD	YPL112c.
SGD	S000006033. PEX25.
Phylogenomic databases
eggNOG	NOG40921.
GeneTree	ENSGT00530000068471.
HOGENOM	HOG000066022.
OMA	ESILLYK.
OrthoDB	EOG4V46J4.
Gene expression databases
Genevestigator	Q02969.
Family and domain databases
InterPro	IPR008733. PEX11. [Graphical view]
Pfam	PF05648. PEX11. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980843.

Entry information

Entry name

PEX25_YEAST

Accession

Primary (citable) accession number: Q02969
Secondary accession number(s): D6W3Q5, Q6B2A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program