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Q02969

- PEX25_YEAST

UniProt

Q02969 - PEX25_YEAST

Protein

Peroxisomal membrane protein PEX25

Gene

PEX25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Required for regulation of peroxisome size and maintenance. Has a role in the import of peroxisomal matrix proteins. Imports RHO1 into the peroxisome. Also promotes peroxisome division and biogenesis.5 Publications

    GO - Biological processi

    1. peroxisome fission Source: UniProtKB
    2. peroxisome organization Source: SGD
    3. protein import into peroxisome matrix Source: SGD
    4. regulation of peroxisome size Source: UniProtKB

    Keywords - Biological processi

    Peroxisome biogenesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34013-MONOMER.

    Protein family/group databases

    TCDBi3.A.20.1.5. the peroxisomal protein importer (ppi) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal membrane protein PEX25
    Alternative name(s):
    Peroxin-25
    Gene namesi
    Name:PEX25
    Ordered Locus Names:YPL112C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL112c.
    SGDiS000006033. PEX25.

    Subcellular locationi

    Peroxisome membrane 4 Publications; Single-pass membrane protein 4 Publications

    GO - Cellular componenti

    1. integral component of peroxisomal membrane Source: InterPro
    2. peroxisomal membrane Source: SGD
    3. peroxisome Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 394394Peroxisomal membrane protein PEX25PRO_0000270568Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei58 – 581Phosphoserine1 Publication
    Modified residuei63 – 631Phosphoserine1 Publication
    Modified residuei289 – 2891Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ02969.
    PaxDbiQ02969.
    PeptideAtlasiQ02969.

    Expressioni

    Gene expression databases

    GenevestigatoriQ02969.

    Interactioni

    Subunit structurei

    Homooligomer. Interacts with PEX27 and PEX34.3 Publications

    Protein-protein interaction databases

    BioGridi36068. 25 interactions.
    DIPiDIP-5292N.
    IntActiQ02969. 5 interactions.
    MINTiMINT-504153.
    STRINGi4932.YPL112C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02969.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 366366CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini384 – 39411LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei367 – 38317HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG40921.
    HOGENOMiHOG000066022.
    OMAiYGIMDEL.
    OrthoDBiEOG7PCJS4.

    Family and domain databases

    InterProiIPR008733. PEX11.
    [Graphical view]
    PfamiPF05648. PEX11. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q02969-1 [UniParc]FASTAAdd to Basket

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    MSQFGTTDIV SGSETPPYSG ASYQDAQDDN THPHSSDAGA EKFSAGSGSE    50
    SHTESSRSDD EDSQAKTKMV DNITILKYIL DSLSGRDKLA KIIKYALDIL 100
    KLFIEKSKRN LTVLDPSVLT YYTKILKNLT VKVALRHPIT VIKVLLLSLL 150
    RNFDKKIDFI SQQLSTFRYI LRFGGTPFRV CSFLGKFNKT RKCNFQIDQI 200
    KKIWFNEASL REFLDLYYGI FDELDLLYKL KIWTNKSFYS FVSRQESLAW 250
    QYDILLSLKD HWLNLQSLQK RQLELEVQLK VQNNALLLSP ILMHQAHKDD 300
    GSQSPIRKQL LNDLNVNNDA EVLIHKQLKA IKDEKTLVYL DIARLSFDCM 350
    ANTSDILNLK TPKGTYAVLS LGSGLTGLVK LWITTKRSLC SSKD 394
    Length:394
    Mass (Da):44,911
    Last modified:November 1, 1996 - v1
    Checksum:iA453AF4B5E41DB68
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti8 – 81D → N in AAT92840. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43503 Genomic DNA. Translation: AAB68249.1.
    AY692821 Genomic DNA. Translation: AAT92840.1.
    BK006949 Genomic DNA. Translation: DAA11321.1.
    PIRiS62009.
    RefSeqiNP_015213.1. NM_001183926.1.

    Genome annotation databases

    EnsemblFungiiYPL112C; YPL112C; YPL112C.
    GeneIDi855991.
    KEGGisce:YPL112C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43503 Genomic DNA. Translation: AAB68249.1 .
    AY692821 Genomic DNA. Translation: AAT92840.1 .
    BK006949 Genomic DNA. Translation: DAA11321.1 .
    PIRi S62009.
    RefSeqi NP_015213.1. NM_001183926.1.

    3D structure databases

    ProteinModelPortali Q02969.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36068. 25 interactions.
    DIPi DIP-5292N.
    IntActi Q02969. 5 interactions.
    MINTi MINT-504153.
    STRINGi 4932.YPL112C.

    Protein family/group databases

    TCDBi 3.A.20.1.5. the peroxisomal protein importer (ppi) family.

    Proteomic databases

    MaxQBi Q02969.
    PaxDbi Q02969.
    PeptideAtlasi Q02969.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL112C ; YPL112C ; YPL112C .
    GeneIDi 855991.
    KEGGi sce:YPL112C.

    Organism-specific databases

    CYGDi YPL112c.
    SGDi S000006033. PEX25.

    Phylogenomic databases

    eggNOGi NOG40921.
    HOGENOMi HOG000066022.
    OMAi YGIMDEL.
    OrthoDBi EOG7PCJS4.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34013-MONOMER.

    Miscellaneous databases

    NextBioi 980843.

    Gene expression databases

    Genevestigatori Q02969.

    Family and domain databases

    InterProi IPR008733. PEX11.
    [Graphical view ]
    Pfami PF05648. PEX11. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Conserved function of pex11p and the novel pex25p and pex27p in peroxisome biogenesis."
      Rottensteiner H., Stein K., Sonnenhol E., Erdmann R.
      Mol. Biol. Cell 14:4316-4328(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 56-70 AND 337-354, FUNCTION, SUBUNIT, INTERACTION WITH PEX27, SUBCELLULAR LOCATION.
      Strain: ATCC 201389 / BY4742.
    5. Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Pex11-related proteins in peroxisome dynamics: a role for the novel peroxin Pex27p in controlling peroxisome size and number in Saccharomyces cerevisiae."
      Tam Y.Y.C., Torres-Guzman J.C., Vizeacoumar F.J., Smith J.J., Marelli M., Aitchison J.D., Rachubinski R.A.
      Mol. Biol. Cell 14:4089-4102(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INTERACTION WITH PEX27.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane."
      Marelli M., Smith J.J., Jung S., Yi E., Nesvizhskii A.I., Christmas R.H., Saleem R.A., Tam Y.Y.C., Fagarasanu A., Goodlett D.R., Aebersold R., Rachubinski R.A., Aitchison J.D.
      J. Cell Biol. 167:1099-1112(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58 AND SER-63, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "The peroxin Pex34p functions with the Pex11 family of peroxisomal divisional proteins to regulate the peroxisome population in yeast."
      Tower R.J., Fagarasanu A., Aitchison J.D., Rachubinski R.A.
      Mol. Biol. Cell 22:1727-1738(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PEX34.

    Entry informationi

    Entry nameiPEX25_YEAST
    AccessioniPrimary (citable) accession number: Q02969
    Secondary accession number(s): D6W3Q5, Q6B2A9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2420 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3