Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q02960

- MIF_CHICK

UniProt

Q02960 - MIF_CHICK

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Macrophage migration inhibitory factor

Gene

MIF

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).By similarity

Catalytic activityi

Keto-phenylpyruvate = enol-phenylpyruvate.
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogenBy similarity
Binding sitei33 – 331SubstrateBy similarity
Binding sitei65 – 651Substrate; via amide nitrogenBy similarity
Binding sitei98 – 981SubstrateBy similarity

GO - Molecular functioni

  1. chemoattractant activity Source: Ensembl
  2. dopachrome isomerase activity Source: UniProtKB-EC
  3. phenylpyruvate tautomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell aging Source: Ensembl
  2. cell proliferation Source: Ensembl
  3. cell surface receptor signaling pathway Source: Ensembl
  4. DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  5. inflammatory response Source: UniProtKB-KW
  6. innate immune response Source: UniProtKB-KW
  7. negative regulation of cell aging Source: Ensembl
  8. negative regulation of cell cycle arrest Source: Ensembl
  9. negative regulation of cellular protein metabolic process Source: Ensembl
  10. negative regulation of DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  11. negative regulation of gene expression Source: Ensembl
  12. negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: Ensembl
  13. negative regulation of mature B cell apoptotic process Source: Ensembl
  14. negative regulation of myeloid cell apoptotic process Source: Ensembl
  15. positive regulation of arachidonic acid secretion Source: Ensembl
  16. positive regulation of B cell proliferation Source: Ensembl
  17. positive regulation of chemokine (C-X-C motif) ligand 2 production Source: Ensembl
  18. positive regulation of cytokine secretion Source: Ensembl
  19. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
  20. positive regulation of fibroblast proliferation Source: Ensembl
  21. positive regulation of lipopolysaccharide-mediated signaling pathway Source: Ensembl
  22. positive regulation of MAP kinase activity Source: Ensembl
  23. positive regulation of myeloid leukocyte cytokine production involved in immune response Source: Ensembl
  24. positive regulation of peptidyl-serine phosphorylation Source: Ensembl
  25. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  26. positive regulation of prostaglandin secretion involved in immune response Source: Ensembl
  27. positive regulation of protein kinase A signaling Source: Ensembl
  28. prostaglandin biosynthetic process Source: Ensembl
  29. protein homotrimerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
Gene namesi
Name:MIF
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 15

Subcellular locationi

Secreted By similarity. Cytoplasm By similarity
Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial antigens (By similarity).By similarity

GO - Cellular componenti

  1. cell surface Source: Ensembl
  2. cytoplasm Source: UniProtKB-KW
  3. extracellular space Source: UniProtKB-KW
  4. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 115114Macrophage migration inhibitory factorPRO_0000158069Add
BLAST

Proteomic databases

PaxDbiQ02960.
PRIDEiQ02960.

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000010214.

Structurei

3D structure databases

ProteinModelPortaliQ02960.
SMRiQ02960. Positions 1-115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.Curated

Phylogenomic databases

eggNOGiNOG08790.
GeneTreeiENSGT00730000111101.
HOGENOMiHOG000112325.
HOVERGENiHBG003240.
InParanoidiQ02960.
KOiK07253.
OMAiKPERYVM.
OrthoDBiEOG7GXPDN.
PhylomeDBiQ02960.
TreeFamiTF313853.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
PROSITEiPS01158. MIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02960-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPMFTIHTNV CKDAVPDSLL GELTQQLAKA TGKPAQYIAV HIVPDQMMSF
60 70 80 90 100
GGSTDPCALC SLYSIGKIGG QQNKTYTKLL CDMIAKHLHV SADRVYINYF
110
DINAANVGWN GSTFA
Length:115
Mass (Da):12,484
Last modified:January 23, 2007 - v3
Checksum:iB85216F4E500B314
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M95776 mRNA. Translation: AAA48939.1.
PIRiC47274.
RefSeqiXP_003642282.1. XM_003642234.2.
UniGeneiGga.3585.

Genome annotation databases

EnsembliENSGALT00000010228; ENSGALP00000010214; ENSGALG00000006326.
GeneIDi100857237.
KEGGigga:100857237.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M95776 mRNA. Translation: AAA48939.1 .
PIRi C47274.
RefSeqi XP_003642282.1. XM_003642234.2.
UniGenei Gga.3585.

3D structure databases

ProteinModelPortali Q02960.
SMRi Q02960. Positions 1-115.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000010214.

Proteomic databases

PaxDbi Q02960.
PRIDEi Q02960.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000010228 ; ENSGALP00000010214 ; ENSGALG00000006326 .
GeneIDi 100857237.
KEGGi gga:100857237.

Organism-specific databases

CTDi 4282.

Phylogenomic databases

eggNOGi NOG08790.
GeneTreei ENSGT00730000111101.
HOGENOMi HOG000112325.
HOVERGENi HBG003240.
InParanoidi Q02960.
KOi K07253.
OMAi KPERYVM.
OrthoDBi EOG7GXPDN.
PhylomeDBi Q02960.
TreeFami TF313853.

Miscellaneous databases

PROi Q02960.

Family and domain databases

InterProi IPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view ]
PANTHERi PTHR11954. PTHR11954. 1 hit.
Pfami PF01187. MIF. 1 hit.
[Graphical view ]
ProDomi PD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF55331. SSF55331. 1 hit.
PROSITEi PS01158. MIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A macrophage migration inhibitory factor is expressed in the differentiating cells of the eye lens."
    Wistow G.J., Shaughnessy M., Lee D.C., Hodin J., Zelenka P.S.
    Proc. Natl. Acad. Sci. U.S.A. 90:1272-1275(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lens.

Entry informationi

Entry nameiMIF_CHICK
AccessioniPrimary (citable) accession number: Q02960
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3