Q02952 (AKA12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
A-kinase anchor protein 12
Short name=AKAP-12

Alternative name(s):
A-kinase anchor protein 250 kDa
Short name=AKAP 250
Gravin
Myasthenia gravis autoantigen

Gene names

Name:	AKAP12
Synonyms:	AKAP250

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1782 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Subunit structure	Binds to dimeric RII-alpha regulatory subunit of PKC.
Subcellular location	Cytoplasm › cell cortex Probable. Cytoplasm › cytoskeleton Probable. Note: May be part of the cortical cytoskeleton.
Tissue specificity	Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
Induction	Activated by lysophosphatidylcholine (lysoPC).
Domain	Polybasic regions located between residues 266 and 557 are involved in binding PKC.
Miscellaneous	Antibodies against the C-terminal of gravin can be produced by patients with myasthenia gravis (MG).
Sequence similarities	Contains 3 AKAP domains.
Sequence caution	The sequence BAE06085.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Traceable author statement PubMed 10858453. Source: ProtInc positive regulation of cAMP biosynthetic process Inferred from mutant phenotype PubMed 17081159. Source: UniProtKB positive regulation of protein kinase A signaling cascade Inferred from mutant phenotype PubMed 17081159. Source: UniProtKB protein targeting Inferred from electronic annotation. Source: InterPro
Cellular_component	cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from direct assay. Source: HPA cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	protein kinase A binding Traceable author statement PubMed 10858453. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EGFR	P00533	2	EBI-2562430,EBI-297353

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q02952-1)

Also known as: Alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q02952-2)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-98: Missing.
     99-106: EEEVIVTE → MLGTITIT

Isoform 3 (identifier: Q02952-3)

Also known as: Gamma;

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.
     106-106: E → M

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1782	1782	A-kinase anchor protein 12		PRO_0000064519
Regions
	Domain	604 – 634	31	AKAP 1
	Domain	753 – 783	31	AKAP 2
	Domain	798 – 828	31	AKAP 3
	Region	266 – 557	292	Involved in PKC-binding Probable
	Region	1541 – 1554	14	RII-binding Probable
	Compositional bias	98 – 101	4	Poly-Glu
Amino acid modifications
	Modified residue	19	1	Phosphoserine By similarity
	Modified residue	75	1	Phosphoserine Ref.14
	Modified residue	96	1	Phosphoserine Ref.14
	Modified residue	219	1	Phosphoserine Ref.9
	Modified residue	371	1	Phosphoserine Ref.14
	Modified residue	374	1	Phosphotyrosine By similarity
	Modified residue	381	1	Phosphoserine Ref.14
	Modified residue	483	1	Phosphoserine Ref.14
	Modified residue	505	1	Phosphoserine Ref.10
	Modified residue	557	1	Phosphoserine Ref.14
	Modified residue	598	1	Phosphoserine Ref.14
	Modified residue	612	1	Phosphoserine Ref.14
	Modified residue	627	1	Phosphoserine Ref.14
	Modified residue	629	1	Phosphoserine By similarity
	Modified residue	645	1	Phosphoserine Ref.14
	Modified residue	646	1	Phosphothreonine By similarity
	Modified residue	648	1	Phosphoserine By similarity
	Modified residue	696	1	Phosphoserine By similarity
	Modified residue	697	1	Phosphoserine By similarity
	Modified residue	698	1	Phosphoserine By similarity
	Modified residue	1331	1	Phosphoserine Ref.14
	Modified residue	1395	1	Phosphoserine Ref.11
	Modified residue	1587	1	Phosphoserine Ref.14
	Lipidation	2	1	N-myristoyl glycine Ref.12
Natural variations
	Alternative sequence	1 – 105	105	Missing in isoform 3.		VSP_028133
	Alternative sequence	1 – 98	98	Missing in isoform 2.		VSP_004110
	Alternative sequence	99 – 106	8	EEEVIVTE → MLGTITIT in isoform 2.		VSP_004111
	Alternative sequence	106	1	E → M in isoform 3.		VSP_028134
	Natural variant	117	1	K → E. Ref.1 Ref.3 Ref.6 Corresponds to variant rs10872670 [ dbSNP | Ensembl ].		VAR_035115
	Natural variant	216	1	K → Q. Ref.1 Ref.3 Ref.4 Ref.6 Corresponds to variant rs3734799 [ dbSNP | Ensembl ].		VAR_035116
	Natural variant	240	1	E → K in a colorectal cancer sample; somatic mutation. Ref.15		VAR_035780
	Natural variant	920	1	E → G. Ref.4 Corresponds to variant rs13212161 [ dbSNP | Ensembl ].		VAR_035117
	Natural variant	987	1	A → S. Ref.1 Corresponds to variant rs1042069 [ dbSNP | Ensembl ].		VAR_056731
	Natural variant	1096	1	V → I. Corresponds to variant rs3734797 [ dbSNP | Ensembl ].		VAR_035118
	Natural variant	1296	1	R → L. Corresponds to variant rs9478198 [ dbSNP | Ensembl ].		VAR_035119
	Natural variant	1355	1	E → K. Ref.4 Corresponds to variant rs12201388 [ dbSNP | Ensembl ].		VAR_035120
	Natural variant	1600	1	E → D. Ref.3 Corresponds to variant rs3823310 [ dbSNP | Ensembl ].		VAR_035121
	Natural variant	1689	1	E → D. Corresponds to variant rs3734795 [ dbSNP | Ensembl ].		VAR_035122
Experimental info
	Sequence conflict	142 – 145	4	TPEI → NRN in AAC51366. Ref.1
	Sequence conflict	423	1	T → I in BAE06085. Ref.3
	Sequence conflict	449	1	E → G in AAC51366. Ref.1
	Sequence conflict	695	1	G → R in AAC51366. Ref.1
	Sequence conflict	868	1	S → G in AAC51366. Ref.1
	Sequence conflict	946	1	E → G in CAH18338. Ref.4
	Sequence conflict	1182	1	A → T in CAH18338. Ref.4
	Sequence conflict	1531	1	E → EE in CAH18338. Ref.4
	Sequence conflict	1531	1	E → EE in BAE06085. Ref.3
	Sequence conflict	1531	1	E → EE in AAB58938. Ref.6
	Sequence conflict	1531	1	E → EE in AAA35931. Ref.7
	Sequence conflict	1582	1	V → M in CAH18338. Ref.4
	Sequence conflict	1582	1	V → M in AAA35931. Ref.7
	Sequence conflict	1602	1	Q → L in BAA19927. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Alpha) [UniParc]. Last modified January 11, 2011. Version 4. Checksum: DB9DBA4647E6DDD5	FASTA	1,782	191,482
	10 20 30 40 50 60 MGAGSSTEQR SPEQPPEGSS TPAEPEPSGG GPSAEAAPDT TADPAIAASD PATKLLQKNG 70 80 90 100 110 120 QLSTINGVAE QDELSLQEGD LNGQKGALNG QGALNSQEEE EVIVTEVGQR DSEDVSKRDS 130 140 150 160 170 180 DKEMATKSAV VHDITDDGQE ETPEIIEQIP SSESNLEELT QPTESQANDI GFKKVFKFVG 190 200 210 220 230 240 FKFTVKKDKT EKPDTVQLLT VKKDEGEGAA GAGDHKDPSL GAGEAASKES EPKQSTEKPE 250 260 270 280 290 300 ETLKREQSHA EISPPAESGQ AVEECKEEGE EKQEKEPSKS AESPTSPVTS ETGSTFKKFF 310 320 330 340 350 360 TQGWAGWRKK TSFRKPKEDE VEASEKKKEQ EPEKVDTEED GKAEVASEKL TASEQAHPQE 370 380 390 400 410 420 PAESAHEPRL SAEYEKVELP SEEQVSGSQG PSEEKPAPLA TEVFDEKIEV HQEEVVAEVH 430 440 450 460 470 480 VSTVEERTEE QKTEVEETAG SVPAEELVEM DAEPQEAEPA KELVKLKETC VSGEDPTQGA 490 500 510 520 530 540 DLSPDEKVLS KPPEGVVSEV EMLSSQERMK VQGSPLKKLF TSTGLKKLSG KKQKGKRGGG 550 560 570 580 590 600 DEESGEHTQV PADSPDSQEE QKGESSASSP EEPEEITCLE KGLAEVQQDG EAEEGATSDG 610 620 630 640 650 660 EKKREGVTPW ASFKKMVTPK KRVRRPSESD KEDELDKVKS ATLSSTESTA SEMQEEMKGS 670 680 690 700 710 720 VEEPKPEEPK RKVDTSVSWE ALICVGSSKK RARRGSSSDE EGGPKAMGGD HQKADEAGKD 730 740 750 760 770 780 KETGTDGILA GSQEHDPGQG SSSPEQAGSP TEGEGVSTWE SFKRLVTPRK KSKSKLEEKS 790 800 810 820 830 840 EDSIAGSGVE HSTPDTEPGK EESWVSIKKF IPGRRKKRPD GKQEQAPVED AGPTGANEDD 850 860 870 880 890 900 SDVPAVVPLS EYDAVEREKM EAQQAQKSAE QPEQKAATEV SKELSESQVH MMAAAVADGT 910 920 930 940 950 960 RAATIIEERS PSWISASVTE PLEQVEAEAA LLTEEVLERE VIAEEEPPTV TEPLPENREA 970 980 990 1000 1010 1020 RGDTVVSEAE LTPEAVTAAE TAGPLGAEEG TEASAAEETT EMVSAVSQLT DSPDTTEEAT 1030 1040 1050 1060 1070 1080 PVQEVEGGVP DIEEQERRTQ EVLQAVAEKV KEESQLPGTG GPEDVLQPVQ RAEAERPEEQ 1090 1100 1110 1120 1130 1140 AEASGLKKET DVVLKVDAQE AKTEPFTQGK VVGQTTPESF EKAPQVTESI ESSELVTTCQ 1150 1160 1170 1180 1190 1200 AETLAGVKSQ EMVMEQAIPP DSVETPTDSE TDGSTPVADF DAPGTTQKDE IVEIHEENEV 1210 1220 1230 1240 1250 1260 ASGTQSGGTE AEAVPAQKER PPAPSSFVFQ EETKEQSKME DTLEHTDKEV SVETVSILSK 1270 1280 1290 1300 1310 1320 TEGTQEADQY ADEKTKDVPF FEGLEGSIDT GITVSREKVT EVALKGEGTE EAECKKDDAL 1330 1340 1350 1360 1370 1380 ELQSHAKSPP SPVEREMVVQ VEREKTEAEP THVNEEKLEH ETAVTVSEEV SKQLLQTVNV 1390 1400 1410 1420 1430 1440 PIIDGAKEVS SLEGSPPPCL GQEEAVCTKI QVQSSEASFT LTAAAEEEKV LGETANILET 1450 1460 1470 1480 1490 1500 GETLEPAGAH LVLEEKSSEK NEDFAAHPGE DAVPTGPDCQ AKSTPVIVSA TTKKGLSSDL 1510 1520 1530 1540 1550 1560 EGEKTTSLKW KSDEVDEQVA CQEVKVSVAI EDLEPENGIL ELETKSSKLV QNIIQTAVDQ 1570 1580 1590 1600 1610 1620 FVRTEETATE MLTSELQTQA HVIKADSQDA GQETEKEGEE PQASAQDETP ITSAKEESES 1630 1640 1650 1660 1670 1680 TAVGQAHSDI SKDMSEASEK TMTVEVEGST VNDQQLEEVV LPSEEEGGGA GTKSVPEDDG 1690 1700 1710 1720 1730 1740 HALLAERIEK SLVEPKEDEK GDDVDDPENQ NSALADTDAS GGLTKESPDT NGPKQKEKED 1750 1760 1770 1780 AQEVELQEGK VHSESDKAIT PQAQEELQKQ ERESAKSELT ES
	Isoform 2 (Beta) [UniParc]. Checksum: 384AEDA06CC83D93	FASTA	1,684	181,690
	10 20 30 40 50 60 MLGTITITVG QRDSEDVSKR DSDKEMATKS AVVHDITDDG QEETPEIIEQ IPSSESNLEE 70 80 90 100 110 120 LTQPTESQAN DIGFKKVFKF VGFKFTVKKD KTEKPDTVQL LTVKKDEGEG AAGAGDHKDP 130 140 150 160 170 180 SLGAGEAASK ESEPKQSTEK PEETLKREQS HAEISPPAES GQAVEECKEE GEEKQEKEPS 190 200 210 220 230 240 KSAESPTSPV TSETGSTFKK FFTQGWAGWR KKTSFRKPKE DEVEASEKKK EQEPEKVDTE 250 260 270 280 290 300 EDGKAEVASE KLTASEQAHP QEPAESAHEP RLSAEYEKVE LPSEEQVSGS QGPSEEKPAP 310 320 330 340 350 360 LATEVFDEKI EVHQEEVVAE VHVSTVEERT EEQKTEVEET AGSVPAEELV EMDAEPQEAE 370 380 390 400 410 420 PAKELVKLKE TCVSGEDPTQ GADLSPDEKV LSKPPEGVVS EVEMLSSQER MKVQGSPLKK 430 440 450 460 470 480 LFTSTGLKKL SGKKQKGKRG GGDEESGEHT QVPADSPDSQ EEQKGESSAS SPEEPEEITC 490 500 510 520 530 540 LEKGLAEVQQ DGEAEEGATS DGEKKREGVT PWASFKKMVT PKKRVRRPSE SDKEDELDKV 550 560 570 580 590 600 KSATLSSTES TASEMQEEMK GSVEEPKPEE PKRKVDTSVS WEALICVGSS KKRARRGSSS 610 620 630 640 650 660 DEEGGPKAMG GDHQKADEAG KDKETGTDGI LAGSQEHDPG QGSSSPEQAG SPTEGEGVST 670 680 690 700 710 720 WESFKRLVTP RKKSKSKLEE KSEDSIAGSG VEHSTPDTEP GKEESWVSIK KFIPGRRKKR 730 740 750 760 770 780 PDGKQEQAPV EDAGPTGANE DDSDVPAVVP LSEYDAVERE KMEAQQAQKS AEQPEQKAAT 790 800 810 820 830 840 EVSKELSESQ VHMMAAAVAD GTRAATIIEE RSPSWISASV TEPLEQVEAE AALLTEEVLE 850 860 870 880 890 900 REVIAEEEPP TVTEPLPENR EARGDTVVSE AELTPEAVTA AETAGPLGAE EGTEASAAEE 910 920 930 940 950 960 TTEMVSAVSQ LTDSPDTTEE ATPVQEVEGG VPDIEEQERR TQEVLQAVAE KVKEESQLPG 970 980 990 1000 1010 1020 TGGPEDVLQP VQRAEAERPE EQAEASGLKK ETDVVLKVDA QEAKTEPFTQ GKVVGQTTPE 1030 1040 1050 1060 1070 1080 SFEKAPQVTE SIESSELVTT CQAETLAGVK SQEMVMEQAI PPDSVETPTD SETDGSTPVA 1090 1100 1110 1120 1130 1140 DFDAPGTTQK DEIVEIHEEN EVASGTQSGG TEAEAVPAQK ERPPAPSSFV FQEETKEQSK 1150 1160 1170 1180 1190 1200 MEDTLEHTDK EVSVETVSIL SKTEGTQEAD QYADEKTKDV PFFEGLEGSI DTGITVSREK 1210 1220 1230 1240 1250 1260 VTEVALKGEG TEEAECKKDD ALELQSHAKS PPSPVEREMV VQVEREKTEA EPTHVNEEKL 1270 1280 1290 1300 1310 1320 EHETAVTVSE EVSKQLLQTV NVPIIDGAKE VSSLEGSPPP CLGQEEAVCT KIQVQSSEAS 1330 1340 1350 1360 1370 1380 FTLTAAAEEE KVLGETANIL ETGETLEPAG AHLVLEEKSS EKNEDFAAHP GEDAVPTGPD 1390 1400 1410 1420 1430 1440 CQAKSTPVIV SATTKKGLSS DLEGEKTTSL KWKSDEVDEQ VACQEVKVSV AIEDLEPENG 1450 1460 1470 1480 1490 1500 ILELETKSSK LVQNIIQTAV DQFVRTEETA TEMLTSELQT QAHVIKADSQ DAGQETEKEG 1510 1520 1530 1540 1550 1560 EEPQASAQDE TPITSAKEES ESTAVGQAHS DISKDMSEAS EKTMTVEVEG STVNDQQLEE 1570 1580 1590 1600 1610 1620 VVLPSEEEGG GAGTKSVPED DGHALLAERI EKSLVEPKED EKGDDVDDPE NQNSALADTD 1630 1640 1650 1660 1670 1680 ASGGLTKESP DTNGPKQKEK EDAQEVELQE GKVHSESDKA ITPQAQEELQ KQERESAKSE LTES
	Isoform 3 (Gamma) [UniParc]. Checksum: 8533672E99E8A1D6	FASTA	1,677	180,990
	10 20 30 40 50 60 MVGQRDSEDV SKRDSDKEMA TKSAVVHDIT DDGQEETPEI IEQIPSSESN LEELTQPTES 70 80 90 100 110 120 QANDIGFKKV FKFVGFKFTV KKDKTEKPDT VQLLTVKKDE GEGAAGAGDH KDPSLGAGEA 130 140 150 160 170 180 ASKESEPKQS TEKPEETLKR EQSHAEISPP AESGQAVEEC KEEGEEKQEK EPSKSAESPT 190 200 210 220 230 240 SPVTSETGST FKKFFTQGWA GWRKKTSFRK PKEDEVEASE KKKEQEPEKV DTEEDGKAEV 250 260 270 280 290 300 ASEKLTASEQ AHPQEPAESA HEPRLSAEYE KVELPSEEQV SGSQGPSEEK PAPLATEVFD 310 320 330 340 350 360 EKIEVHQEEV VAEVHVSTVE ERTEEQKTEV EETAGSVPAE ELVEMDAEPQ EAEPAKELVK 370 380 390 400 410 420 LKETCVSGED PTQGADLSPD EKVLSKPPEG VVSEVEMLSS QERMKVQGSP LKKLFTSTGL 430 440 450 460 470 480 KKLSGKKQKG KRGGGDEESG EHTQVPADSP DSQEEQKGES SASSPEEPEE ITCLEKGLAE 490 500 510 520 530 540 VQQDGEAEEG ATSDGEKKRE GVTPWASFKK MVTPKKRVRR PSESDKEDEL DKVKSATLSS 550 560 570 580 590 600 TESTASEMQE EMKGSVEEPK PEEPKRKVDT SVSWEALICV GSSKKRARRG SSSDEEGGPK 610 620 630 640 650 660 AMGGDHQKAD EAGKDKETGT DGILAGSQEH DPGQGSSSPE QAGSPTEGEG VSTWESFKRL 670 680 690 700 710 720 VTPRKKSKSK LEEKSEDSIA GSGVEHSTPD TEPGKEESWV SIKKFIPGRR KKRPDGKQEQ 730 740 750 760 770 780 APVEDAGPTG ANEDDSDVPA VVPLSEYDAV EREKMEAQQA QKSAEQPEQK AATEVSKELS 790 800 810 820 830 840 ESQVHMMAAA VADGTRAATI IEERSPSWIS ASVTEPLEQV EAEAALLTEE VLEREVIAEE 850 860 870 880 890 900 EPPTVTEPLP ENREARGDTV VSEAELTPEA VTAAETAGPL GAEEGTEASA AEETTEMVSA 910 920 930 940 950 960 VSQLTDSPDT TEEATPVQEV EGGVPDIEEQ ERRTQEVLQA VAEKVKEESQ LPGTGGPEDV 970 980 990 1000 1010 1020 LQPVQRAEAE RPEEQAEASG LKKETDVVLK VDAQEAKTEP FTQGKVVGQT TPESFEKAPQ 1030 1040 1050 1060 1070 1080 VTESIESSEL VTTCQAETLA GVKSQEMVME QAIPPDSVET PTDSETDGST PVADFDAPGT 1090 1100 1110 1120 1130 1140 TQKDEIVEIH EENEVASGTQ SGGTEAEAVP AQKERPPAPS SFVFQEETKE QSKMEDTLEH 1150 1160 1170 1180 1190 1200 TDKEVSVETV SILSKTEGTQ EADQYADEKT KDVPFFEGLE GSIDTGITVS REKVTEVALK 1210 1220 1230 1240 1250 1260 GEGTEEAECK KDDALELQSH AKSPPSPVER EMVVQVEREK TEAEPTHVNE EKLEHETAVT 1270 1280 1290 1300 1310 1320 VSEEVSKQLL QTVNVPIIDG AKEVSSLEGS PPPCLGQEEA VCTKIQVQSS EASFTLTAAA 1330 1340 1350 1360 1370 1380 EEEKVLGETA NILETGETLE PAGAHLVLEE KSSEKNEDFA AHPGEDAVPT GPDCQAKSTP 1390 1400 1410 1420 1430 1440 VIVSATTKKG LSSDLEGEKT TSLKWKSDEV DEQVACQEVK VSVAIEDLEP ENGILELETK 1450 1460 1470 1480 1490 1500 SSKLVQNIIQ TAVDQFVRTE ETATEMLTSE LQTQAHVIKA DSQDAGQETE KEGEEPQASA 1510 1520 1530 1540 1550 1560 QDETPITSAK EESESTAVGQ AHSDISKDMS EASEKTMTVE VEGSTVNDQQ LEEVVLPSEE 1570 1580 1590 1600 1610 1620 EGGGAGTKSV PEDDGHALLA ERIEKSLVEP KEDEKGDDVD DPENQNSALA DTDASGGLTK 1630 1640 1650 1660 1670 ESPDTNGPKQ KEKEDAQEVE LQEGKVHSES DKAITPQAQE ELQKQERESA KSELTES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein." Nauert J.B., Klauck T.M., Langeberg L.K., Scott J.D. Curr. Biol. 7:52-62(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS GLU-117; GLN-216 AND SER-987. Tissue: Heart.
[2]	"Changes of gene expression by lysophosphatidylcholine in vascular endothelial cells: 12 up-regulated distinct genes including 5 cell growth-related, 3 thrombosis-related, and 4 others." Sato N., Kokame K., Shimokado K., Kato H., Miyata T. J. Biochem. 123:1119-1126(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Umbilical vein endothelial cell.
[3]	"Preparation of a set of expression-ready clones of mammalian long cDNAs encoding large proteins by the ORF trap cloning method." Nakajima D., Saito K., Yamakawa H., Kikuno R.F., Nakayama M., Ohara R., Okazaki N., Koga H., Nagase T., Ohara O. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS GLU-117; GLN-216 AND ASP-1600. Tissue: Brain.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANTS GLN-216; GLY-920 AND LYS-1355. Tissue: Testis.
[5]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Sequence of gravin cDNA isolated from a human umbilical vein endothelial cell library." Bowditch R.D., Ginsberg M.H. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 43-1782, VARIANTS GLU-117 AND GLN-216. Tissue: Umbilical vein endothelial cell.
[7]	"Molecular cloning and preliminary characterization of a novel cytoplasmic antigen recognized by myasthenia gravis sera." Gordon T., Grove B., Loftus J.C., O'Toole T., McMillan R., Lindstrom J., Ginsberg M.H. J. Clin. Invest. 90:992-999(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1478-1782. Tissue: Umbilical vein endothelial cell.
[8]	"Multiple promoters direct expression of three AKAP12 isoforms with distinct subcellular and tissue distribution profiles." Streb J.W., Kitchen C.M., Gelman I.H., Miano J.M. J. Biol. Chem. 279:56014-56023(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-219, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[11]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1395, MASS SPECTROMETRY.
[12]	"Strategy for comprehensive identification of human N-myristoylated proteins using an insect cell-free protein synthesis system." Suzuki T., Moriya K., Nagatoshi K., Ota Y., Ezure T., Ando E., Tsunasawa S., Utsumi T. Proteomics 10:1780-1793(2010) [PubMed] [Europe PMC] [Abstract] Cited for: MYRISTOYLATION AT GLY-2.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75; SER-96; SER-371; SER-381; SER-483; SER-557; SER-598; SER-612; SER-627; SER-645; SER-1331 AND SER-1587, MASS SPECTROMETRY.
[15]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-240.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	U81607 mRNA. Translation: AAC51366.1. AB003476 mRNA. Translation: BAA19927.1. AB210003 mRNA. Translation: BAE06085.1. Different initiation. CR749527 mRNA. Translation: CAH18338.1. AL590413, AL033392, AL356535 Genomic DNA. Translation: CAI13590.1. AL356535, AL033392, AL590413 Genomic DNA. Translation: CAI16151.1. AL033392, AL356535, AL590413 Genomic DNA. Translation: CAI20467.1. AL590413, AL033392 Genomic DNA. Translation: CAI13591.1. AL033392, AL590413 Genomic DNA. Translation: CAI20468.1. AF001504 mRNA. Translation: AAB58938.1. M96322 mRNA. Translation: AAA35931.1.
IPI	IPI00237884. IPI00867627. IPI00940222.
PIR	A43922. JW0057.
RefSeq	NP_005091.2. NM_005100.3. NP_653080.1. NM_144497.2.
UniGene	Hs.371240.
3D structure databases
ProteinModelPortal	Q02952.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q02952. 5 interactions.
MINT	MINT-2411728.
STRING	9606.ENSP00000253332.
PTM databases
PhosphoSite	Q02952.
Polymorphism databases
DMDM	158931155.
Proteomic databases
PaxDb	Q02952.
PRIDE	Q02952.
Protocols and materials databases
DNASU	9590.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000253332; ENSP00000253332; ENSG00000131016. ENST00000354675; ENSP00000346702; ENSG00000131016. ENST00000359755; ENSP00000352794; ENSG00000131016. ENST00000402676; ENSP00000384537; ENSG00000131016.
GeneID	9590.
KEGG	hsa:9590.
UCSC	uc003qoe.3. human. uc003qof.3. human. uc003qog.3. human.
Organism-specific databases
CTD	9590.
GeneCards	GC06P151561.
H-InvDB	HIX0025002.
HGNC	HGNC:370. AKAP12.
HPA	HPA006344.
MIM	604698. gene.
neXtProt	NX_Q02952.
PharmGKB	PA24664.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG12793.
HOVERGEN	HBG050472.
InParanoid	Q02952.
KO	K16528.
OMA	EQAGSPT.
OrthoDB	EOG408N80.
PhylomeDB	Q02952.
Gene expression databases
Bgee	Q02952.
CleanEx	HS_AKAP12.
Genevestigator	Q02952.
GermOnline	ENSG00000131016. Homo sapiens.
Family and domain databases
InterPro	IPR001573. Pkinase-A_anch_WSK-motif. IPR018459. RII_binding_1. [Graphical view]
Pfam	PF10522. RII_binding_1. 1 hit. PF03832. WSK. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	AKAP12. human.
GenomeRNAi	9590.
NextBio	35991.
SOURCE	Search...

Entry information

Entry name

AKA12_HUMAN

Accession

Primary (citable) accession number: Q02952
Secondary accession number(s): O00310 , O00498, Q4LE68, Q5SZ80, Q5TGN1, Q68D82, Q99970

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 120 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.