Vacuolar protein sorting-associated protein 30 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q02948 (VPS30_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 30

Alternative name(s):
Autophagy-related protein 6

Gene names

Name:	VPS30
Synonyms:	APG6, ATG6, VPT30
Ordered Locus Names:	YPL120W
ORF Names:	LPH7

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Ref.1 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8
Subunit structure	Component of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34 and ATG14; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38.
Subcellular location	Endosome membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein Ref.1 Ref.5 Ref.7 Ref.9.
Miscellaneous	Present with 2490 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the beclin family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Endosome Membrane Vacuole
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	CVT pathway Inferred from mutant phenotype Ref.5 Ref.1. Source: SGD ER-associated protein catabolic process Inferred from mutant phenotype. Source: SGD late endosome to vacuole transport Inferred from mutant phenotype Ref.7. Source: SGD macroautophagy Inferred from mutant phenotype Ref.7. Source: SGD peroxisome degradation Inferred from mutant phenotype. Source: SGD phosphatidylinositol biosynthetic process Inferred from mutant phenotype Ref.8. Source: SGD piecemeal microautophagy of nucleus Inferred from mutant phenotype. Source: SGD retrograde transport, endosome to Golgi Inferred from mutant phenotype Ref.8. Source: SGD
Cellular component	endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell peripheral to membrane of membrane fraction Inferred from direct assay Ref.5. Source: SGD phosphatidylinositol 3-kinase complex I Inferred from direct assay Ref.7. Source: SGD phosphatidylinositol 3-kinase complex II Inferred from direct assay Ref.7. Source: SGD vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG14	P38270	7	EBI-2670,EBI-2699
VPS38	Q05919	8	EBI-2670,EBI-29972

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 557

557

Vacuolar protein sorting-associated protein 30

PRO_0000218559

Regions

Coiled coil

189 – 322

134

Potential

Amino acid modifications

Modified residue

142

Phosphothreonine Ref.11

Modified residue

544

Phosphoserine Ref.11

Sequences

Sequence

Length

Mass (Da)

Tools

Q02948 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4BA74B614CABE1C0
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FASTA

557

63,261

        10         20         30         40         50         60 
MKCQTCHLPL QLDPSLEGLS LTQRNLLLSN NSIITATNEN VISNKGIEAA DNCGPQIPKE 

        70         80         90        100        110        120 
RLRRLGEIQN IKDLNLKDDK LITDSFVFLN HDDDDNANIT SNSREDQRYG NANGNDNKKA 

       130        140        150        160        170        180 
NSDTSDGTST FRDHDEEEQE ATDEDENQQI QLNSKTLSTQ VNAMTNVFNI LSSQTNIDFP 

       190        200        210        220        230        240 
ICQDCCNILI NRLKSEYDDA IKERDTYAQF LSKLESQNKE ISESNKEKQY SHNLSEKENL 

       250        260        270        280        290        300 
KKEEERLLDQ LLRLEMTDDD LDGELVRLQE KKVQLENEKL QKLSDQNLMD LNNIQFNKNL 

       310        320        330        340        350        360 
QSLKLQYELS LNQLDKLRKI NIFNATFKIS HSGPFATING LRLGSIPESV VPWKEINAAL 

       370        380        390        400        410        420 
GQLILLLATI NKNLKINLVD YELQPMGSFS KIKKRMVNSV EYNNSTTNAP GDWLILPVYY 

       430        440        450        460        470        480 
DENFNLGRIF RKETKFDKSL ETTLEIISEI TRQLSTIASS YSSQTLTTSQ DESSMNNAND 

       490        500        510        520        530        540 
VENSTSILEL PYIMNKDKIN GLSVKLHGSS PNLEWTTAMK FLLTNVKWLL AFSSNLLSKS 

       550 
ITLSPTVNYN DKTISGN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae." Kametaka S., Okano T., Ohsumi M., Ohsumi Y. J. Biol. Chem. 273:22284-22291(1998) [PubMed: 9712845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG14.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae." Tsukada M., Ohsumi Y. FEBS Lett. 333:169-174(1993) [PubMed: 8224160] [Abstract] Cited for: FUNCTION.
[5]	"Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products." Seaman M.N.J., Marcusson E.G., Cereghino J.L., Emr S.D. J. Cell Biol. 137:79-92(1997) [PubMed: 9105038] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation." Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y. EMBO J. 20:5971-5981(2001) [PubMed: 11689437] [Abstract] Cited for: FUNCTION.
[7]	"Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae." Kihara A., Noda T., Ishihara N., Ohsumi Y. J. Cell Biol. 152:519-530(2001) [PubMed: 11157979] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS15; VPS34; VPS38 AND ATG14.
[8]	"Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase." Burda P., Padilla S.M., Sarkar S., Emr S.D. J. Cell Sci. 115:3889-3900(2002) [PubMed: 12244127] [Abstract] Cited for: FUNCTION.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-142 AND SER-544, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011072 Genomic DNA. Translation: BAA32104.1. U43503 Genomic DNA. Translation: AAB68242.1. BK006949 Genomic DNA. Translation: DAA11313.1.
PIR	S62002.
RefSeq	NP_015205.1. NM_001183934.1.
3D structure databases
ProteinModelPortal	Q02948.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2961N.
IntAct	Q02948. 10 interactions.
MINT	MINT-533816.
STRING	Q02948.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL120W; YPL120W; YPL120W.
GeneID	855983.
KEGG	sce:YPL120W.
NMPDR	fig\|4932.3.peg.6336.
Organism-specific databases
CYGD	YPL120w.
SGD	S000006041. VPS30.
Phylogenomic databases
eggNOG	fuNOG05957.
GeneTree	EFGT00050000006761.
HOGENOM	HBG397104.
OMA	NLEWTTA.
OrthoDB	EOG4Z39P5.
Gene expression databases
ArrayExpress	Q02948.
Genevestigator	Q02948.
GermOnline	YPL120W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007243. Autophagy-rel_prot_6. [Graphical view]
KO	K08334.
PANTHER	PTHR12768. APG6. 1 hit.
Pfam	PF04111. APG6. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980822.

Entry information

Entry name

VPS30_YEAST

Accession

Primary (citable) accession number: Q02948
Secondary accession number(s): D6W3P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents