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Protein

General transcription and DNA repair factor IIH subunit TFB2

Gene

TFB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.3 Publications

Miscellaneous

Present with 8900 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • nucleotide-excision repair Source: SGD
  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • transcription by RNA polymerase II Source: SGD

Keywordsi

Biological processDNA damage, DNA excision, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34021-MONOMER
ReactomeiR-SCE-113418 Formation of the Early Elongation Complex
R-SCE-5696395 Formation of Incision Complex in GG-NER
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6781823 Formation of TC-NER Pre-Incision Complex
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SCE-72086 mRNA Capping
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SCE-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription and DNA repair factor IIH subunit TFB2
Short name:
TFIIH subunit TFB2
Alternative name(s):
RNA polymerase II transcription factor B 52 kDa subunit
RNA polymerase II transcription factor B p52 subunit
RNA polymerase II transcription factor B subunit 2
Gene namesi
Name:TFB2
Ordered Locus Names:YPL122C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL122C
SGDiS000006043 TFB2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192681 – 513General transcription and DNA repair factor IIH subunit TFB2Add BLAST513

Proteomic databases

MaxQBiQ02939
PaxDbiQ02939
PRIDEiQ02939

PTM databases

iPTMnetiQ02939

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription (PubMed:7961739, PubMed:14500720, PubMed:9235928). Interacts with TFB5 (PubMed:19172752).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TFB5Q3E7C16EBI-2345544,EBI-2095127

Protein-protein interaction databases

BioGridi36059, 162 interactors
ComplexPortaliCPX-1659 General transcription factor complex TFIIH
DIPiDIP-5783N
IntActiQ02939, 13 interactors
MINTiQ02939
STRINGi4932.YPL122C

Structurei

Secondary structure

1513
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi448 – 450Combined sources3
Beta strandi452 – 460Combined sources9
Helixi465 – 477Combined sources13
Beta strandi481 – 485Combined sources5
Turni486 – 489Combined sources4
Beta strandi490 – 494Combined sources5
Helixi495 – 497Combined sources3
Helixi498 – 505Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DGPX-ray1.80A435-513[»]
3DOMX-ray2.60A/C412-513[»]
5FMFelectron microscopy6.00W447-508[»]
5OQJelectron microscopy4.7021-513[»]
5OQMelectron microscopy5.8021-513[»]
5SVAelectron microscopy15.30a1-513[»]
ProteinModelPortaliQ02939
SMRiQ02939
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02939

Family & Domainsi

Sequence similaritiesi

Belongs to the TFB2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014159
HOGENOMiHOG000170529
InParanoidiQ02939
KOiK03144
OMAiYLEQYAH
OrthoDBiEOG092C3703

Family and domain databases

InterProiView protein in InterPro
IPR004598 TFIIH_p52/Tfb2
PANTHERiPTHR13152 PTHR13152, 1 hit
PfamiView protein in Pfam
PF03849 Tfb2, 1 hit
TIGRFAMsiTIGR00625 tfb2, 1 hit

Sequencei

Sequence statusi: Complete.

Q02939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYSLKHSV TQYLEEIPQQ VQNRLYTSPA TCLAIYRILP PLAKFFIMAM
60 70 80 90 100
VFNENEVPLL DLDKWVNSNG KLQFQNAIKS MKSLHLLIPN KSSGTLMINL
110 120 130 140 150
NPTFKISLRN ALTGGEVQNS FGVVVEENVV SLDLLDEYSA NKWETILHFM
160 170 180 190 200
VGTPLAKIPS EKVLNLLKHS KLMEEVNSTG EFKITNEGFQ FLLQEINSQL
210 220 230 240 250
WTLLLQYLKM IETSKMDLVD VLHFIFMLGA LEVGKAYKID ALSETQRIML
260 270 280 290 300
QDMRDYGLVF QKHSNDSIFY PTKLALMLTS DTKTIRSASN AMDSVLRQNR
310 320 330 340 350
EEPSVNEDGA NGKSTTDITT SDDLNKAGLK NQDIPDGSLI VETNFKIYSY
360 370 380 390 400
SNSPLQIAVL SLFVHLKARF VNMVLGQITR ESIRRALTNG ITADQIIAYL
410 420 430 440 450
ETHAHPQMRR LAEEKLEKKL ELDPNCKEPL QVLPPTVVDQ IRLWQLELDR
460 470 480 490 500
VITYEGSLYS DFETSQEYNL LSKYAQDIGV LLWKDDKKKK FFISKEGNSQ
510
VLDFAKRKLK KKQ
Length:513
Mass (Da):58,535
Last modified:November 1, 1996 - v1
Checksum:i9E440B566396A95A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62804 Genomic DNA Translation: AAB40628.1
U43503 Genomic DNA Translation: AAB68240.1
BK006949 Genomic DNA Translation: DAA11311.1
PIRiS62000
RefSeqiNP_015203.1, NM_001183936.1

Genome annotation databases

EnsemblFungiiYPL122C; YPL122C; YPL122C
GeneIDi855981
KEGGisce:YPL122C

Similar proteinsi

Entry informationi

Entry nameiTFB2_YEAST
AccessioniPrimary (citable) accession number: Q02939
Secondary accession number(s): D6W3P5, P87331
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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