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Protein

RNA polymerase II transcription factor B subunit 2

Gene

TFB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the general transcription and DNA repair factor IIH (TFIIH) core, which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro.3 Publications

GO - Molecular functioni

GO - Biological processi

  • nucleotide-excision repair Source: SGD
  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34021-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-72086. mRNA Capping.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II transcription factor B subunit 2
Alternative name(s):
General transcription and DNA repair factor IIH subunit TFB2
Short name:
TFIIH subunit TFB2
RNA polymerase II transcription factor B 52 kDa subunit
RNA polymerase II transcription factor B p52 subunit
Gene namesi
Name:TFB2
Ordered Locus Names:YPL122C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL122C.
SGDiS000006043. TFB2.

Subcellular locationi

GO - Cellular componenti

  • core TFIIH complex Source: SGD
  • holo TFIIH complex Source: SGD
  • nucleotide-excision repair factor 3 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 513513RNA polymerase II transcription factor B subunit 2PRO_0000119268Add
BLAST

Proteomic databases

MaxQBiQ02939.

PTM databases

iPTMnetiQ02939.

Interactioni

Subunit structurei

Component of the TFIIH core complex, which is composed of RAD3, SSL1, SSL2, TFB1, TFB2, TFB4 and TFB5.2 Publications

Protein-protein interaction databases

BioGridi36059. 40 interactions.
DIPiDIP-5783N.
IntActiQ02939. 9 interactions.
MINTiMINT-2785444.

Structurei

Secondary structure

1
513
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi448 – 4503Combined sources
Beta strandi452 – 4609Combined sources
Helixi465 – 47713Combined sources
Beta strandi481 – 4855Combined sources
Turni486 – 4894Combined sources
Beta strandi490 – 4945Combined sources
Helixi495 – 4973Combined sources
Helixi498 – 5058Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DGPX-ray1.80A435-513[»]
3DOMX-ray2.60A/C412-513[»]
5FMFelectron microscopy6.00W447-508[»]
ProteinModelPortaliQ02939.
SMRiQ02939. Positions 431-507.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02939.

Family & Domainsi

Sequence similaritiesi

Belongs to the TFB2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014159.
HOGENOMiHOG000170529.
InParanoidiQ02939.
KOiK03144.
OMAiHAREQWE.
OrthoDBiEOG092C3703.

Family and domain databases

InterProiIPR004598. TFIIH_p52/Tfb2.
[Graphical view]
PANTHERiPTHR13152. PTHR13152. 3 hits.
PfamiPF03849. Tfb2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00625. tfb2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYSLKHSV TQYLEEIPQQ VQNRLYTSPA TCLAIYRILP PLAKFFIMAM
60 70 80 90 100
VFNENEVPLL DLDKWVNSNG KLQFQNAIKS MKSLHLLIPN KSSGTLMINL
110 120 130 140 150
NPTFKISLRN ALTGGEVQNS FGVVVEENVV SLDLLDEYSA NKWETILHFM
160 170 180 190 200
VGTPLAKIPS EKVLNLLKHS KLMEEVNSTG EFKITNEGFQ FLLQEINSQL
210 220 230 240 250
WTLLLQYLKM IETSKMDLVD VLHFIFMLGA LEVGKAYKID ALSETQRIML
260 270 280 290 300
QDMRDYGLVF QKHSNDSIFY PTKLALMLTS DTKTIRSASN AMDSVLRQNR
310 320 330 340 350
EEPSVNEDGA NGKSTTDITT SDDLNKAGLK NQDIPDGSLI VETNFKIYSY
360 370 380 390 400
SNSPLQIAVL SLFVHLKARF VNMVLGQITR ESIRRALTNG ITADQIIAYL
410 420 430 440 450
ETHAHPQMRR LAEEKLEKKL ELDPNCKEPL QVLPPTVVDQ IRLWQLELDR
460 470 480 490 500
VITYEGSLYS DFETSQEYNL LSKYAQDIGV LLWKDDKKKK FFISKEGNSQ
510
VLDFAKRKLK KKQ
Length:513
Mass (Da):58,535
Last modified:November 1, 1996 - v1
Checksum:i9E440B566396A95A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62804 Genomic DNA. Translation: AAB40628.1.
U43503 Genomic DNA. Translation: AAB68240.1.
BK006949 Genomic DNA. Translation: DAA11311.1.
PIRiS62000.
RefSeqiNP_015203.1. NM_001183936.1.

Genome annotation databases

EnsemblFungiiYPL122C; YPL122C; YPL122C.
GeneIDi855981.
KEGGisce:YPL122C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62804 Genomic DNA. Translation: AAB40628.1.
U43503 Genomic DNA. Translation: AAB68240.1.
BK006949 Genomic DNA. Translation: DAA11311.1.
PIRiS62000.
RefSeqiNP_015203.1. NM_001183936.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DGPX-ray1.80A435-513[»]
3DOMX-ray2.60A/C412-513[»]
5FMFelectron microscopy6.00W447-508[»]
ProteinModelPortaliQ02939.
SMRiQ02939. Positions 431-507.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36059. 40 interactions.
DIPiDIP-5783N.
IntActiQ02939. 9 interactions.
MINTiMINT-2785444.

PTM databases

iPTMnetiQ02939.

Proteomic databases

MaxQBiQ02939.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL122C; YPL122C; YPL122C.
GeneIDi855981.
KEGGisce:YPL122C.

Organism-specific databases

EuPathDBiFungiDB:YPL122C.
SGDiS000006043. TFB2.

Phylogenomic databases

GeneTreeiENSGT00390000014159.
HOGENOMiHOG000170529.
InParanoidiQ02939.
KOiK03144.
OMAiHAREQWE.
OrthoDBiEOG092C3703.

Enzyme and pathway databases

BioCyciYEAST:G3O-34021-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-72086. mRNA Capping.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

EvolutionaryTraceiQ02939.
PROiQ02939.

Family and domain databases

InterProiIPR004598. TFIIH_p52/Tfb2.
[Graphical view]
PANTHERiPTHR13152. PTHR13152. 3 hits.
PfamiPF03849. Tfb2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00625. tfb2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTFB2_YEAST
AccessioniPrimary (citable) accession number: Q02939
Secondary accession number(s): D6W3P5, P87331
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.