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Q02934

- GUNI_CLOTH

UniProt

Q02934 - GUNI_CLOTH

Protein

Endoglucanase 1

Gene

celI

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (17 Apr 2007)
      Previous versions | rss
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    Functioni

    This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. Principally active against barley beta-glucan.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei448 – 4481By similarity
    Active sitei486 – 4861By similarity
    Active sitei495 – 4951By similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC
    2. cellulose binding Source: InterPro
    3. identical protein binding Source: IntAct

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCTHE203119:GIW8-39-MONOMER.
    UniPathwayiUPA00696.

    Protein family/group databases

    CAZyiCBM3. Carbohydrate-Binding Module Family 3.
    GH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase 1 (EC:3.2.1.4)
    Alternative name(s):
    Cellulase I
    Endo-1,4-beta-glucanase
    Endoglucanase I
    Short name:
    EGI
    Gene namesi
    Name:celI
    Ordered Locus Names:Cthe_0040
    OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
    Taxonomic identifieri203119 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
    ProteomesiUP000002145: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 55551 PublicationAdd
    BLAST
    Chaini56 – 887832Endoglucanase 1PRO_0000007951Add
    BLAST

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-8601842,EBI-8601842

    Protein-protein interaction databases

    MINTiMINT-6946608.
    STRINGi203119.Cthe_0040.

    Structurei

    Secondary structure

    1
    887
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi78 – 9114
    Turni99 – 1013
    Beta strandi103 – 1064
    Turni112 – 1154
    Helixi116 – 1183
    Beta strandi129 – 1313
    Helixi136 – 15217
    Helixi154 – 1596
    Helixi163 – 17917
    Beta strandi186 – 1927
    Helixi194 – 1985
    Helixi204 – 2063
    Beta strandi213 – 2208
    Helixi223 – 23917
    Turni240 – 2434
    Helixi245 – 26521
    Turni273 – 2775
    Helixi285 – 29915
    Helixi302 – 3109
    Turni311 – 3144
    Beta strandi321 – 3244
    Helixi336 – 34712
    Helixi352 – 36413
    Beta strandi385 – 3873
    Helixi388 – 40316
    Helixi410 – 42819
    Turni429 – 4313
    Beta strandi439 – 4424
    Helixi450 – 4534
    Beta strandi456 – 4583
    Beta strandi462 – 4654
    Helixi491 – 4944
    Helixi498 – 51518
    Beta strandi533 – 54412
    Beta strandi547 – 55610
    Beta strandi565 – 57511
    Helixi577 – 5804
    Turni581 – 5833
    Helixi586 – 5883
    Beta strandi590 – 5923
    Beta strandi605 – 6084
    Beta strandi611 – 6177
    Beta strandi625 – 6273
    Turni628 – 6303
    Beta strandi631 – 64010
    Helixi649 – 6513
    Helixi653 – 6553
    Beta strandi671 – 6733
    Beta strandi676 – 6805

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2XFGX-ray1.68A54-516[»]
    B517-683[»]
    ProteinModelPortaliQ02934.
    SMRiQ02934. Positions 76-683.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ02934.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini529 – 684156CBM3 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini736 – 887152CBM3 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni56 – 518463CatalyticAdd
    BLAST

    Sequence similaritiesi

    Contains 2 CBM3 (carbohydrate binding type-3) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG2730.
    HOGENOMiHOG000021032.
    KOiK01179.
    K01225.
    OMAiRKEVQFR.
    OrthoDBiEOG6KQ6BP.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    2.60.40.710. 2 hits.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR001956. CBD_3.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view]
    PfamiPF00942. CBM_3. 2 hits.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SMARTiSM01067. CBM_3. 2 hits.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF49384. SSF49384. 2 hits.
    PROSITEiPS51172. CBM3. 2 hits.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q02934-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLVNSLGRR KILLILAVIV AFSTVLLFAK LWGRKTSSTL DEVGSKTHGD    50
    LTAENKNGGY LPEEEIPDQP PATGAFNYGE ALQKAIFFYE CQRSGKLDPS 100
    TLRLNWRGDS GLDDGKDAGI DLTGGWYDAG DHVKFNLPMS YSAAMLGWAV 150
    YEYEDAFKQS GQYNHILNNI KWACDYFIKC HPEKDVYYYQ VGDGHADHAW 200
    WGPAEVMPME RPSYKVDRSS PGSTVVAETS AALAIASIIF KKVDGEYSKE 250
    CLKHAKELFE FADTTKSDDG YTAANGFYNS WSGFYDELSW AAVWLYLATN 300
    DSSYLDKAES YSDKWGYEPQ TNIPKYKWAQ CWDDVTYGTY LLLARIKNDN 350
    GKYKEAIERH LDWWTTGYNG ERITYTPKGL AWLDQWGSLR YATTTAFLAC 400
    VYSDWENGDK EKAKTYLEFA RSQADYALGS TGRSFVVGFG ENPPKRPHHR 450
    TAHGSWADSQ MEPPEHRHVL YGALVGGPDS TDNYTDDISN YTCNEVACDY 500
    NAGFVGLLAK MYKLYGGSPD PKFNGIEEVP EDEIFVEAGV NASGNNFIEI 550
    KAIVNNKSGW PARVCENLSF RYFINIEEIV NAGKSASDLQ VSSSYNQGAK 600
    LSDVKHYKDN IYYVEVDLSG TKIYPGGQSA YKKEVQFRIS APEGTVFNPE 650
    NDYSYQGLSA GTVVKSEYIP VYDAGVLVFG REPGSASKST SKDNGLSKAT 700
    PTVKTESQPT AKHTQNPASD FKTPANQNSV KKDQGIKGEV VLQYANGNAG 750
    ATSNSINPRF KIINNGTKAI NLSDVKIRYY YTKEGGASQN FWCDWSSAGN 800
    SNVTGNFFNL SSPKEGADTC LEVGFGSGAG TLDPGGSVEV QIRFSKEDWS 850
    NYNQSNDYSF NPSASDYTDW NRVTLYISNK LVYGKEP 887
    Length:887
    Mass (Da):98,531
    Last modified:April 17, 2007 - v2
    Checksum:iE587626445BA7A95
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti861 – 88727NPSAS…YGKEP → KQACLRQRTLIYLYATWLR in AAA20892. (PubMed:8436949)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04735 Genomic DNA. Translation: AAA20892.1.
    CP000568 Genomic DNA. Translation: ABN51281.1.
    PIRiA47704.
    RefSeqiYP_001036474.1. NC_009012.1.

    Genome annotation databases

    EnsemblBacteriaiABN51281; ABN51281; Cthe_0040.
    GeneIDi4808805.
    KEGGicth:Cthe_0040.
    PATRICi19513685. VBICloThe47081_0045.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04735 Genomic DNA. Translation: AAA20892.1 .
    CP000568 Genomic DNA. Translation: ABN51281.1 .
    PIRi A47704.
    RefSeqi YP_001036474.1. NC_009012.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2XFG X-ray 1.68 A 54-516 [» ]
    B 517-683 [» ]
    ProteinModelPortali Q02934.
    SMRi Q02934. Positions 76-683.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-6946608.
    STRINGi 203119.Cthe_0040.

    Protein family/group databases

    CAZyi CBM3. Carbohydrate-Binding Module Family 3.
    GH9. Glycoside Hydrolase Family 9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN51281 ; ABN51281 ; Cthe_0040 .
    GeneIDi 4808805.
    KEGGi cth:Cthe_0040.
    PATRICi 19513685. VBICloThe47081_0045.

    Phylogenomic databases

    eggNOGi COG2730.
    HOGENOMi HOG000021032.
    KOi K01179.
    K01225.
    OMAi RKEVQFR.
    OrthoDBi EOG6KQ6BP.

    Enzyme and pathway databases

    UniPathwayi UPA00696 .
    BioCyci CTHE203119:GIW8-39-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q02934.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    2.60.40.710. 2 hits.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR001956. CBD_3.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view ]
    Pfami PF00942. CBM_3. 2 hits.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SMARTi SM01067. CBM_3. 2 hits.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF49384. SSF49384. 2 hits.
    PROSITEi PS51172. CBM3. 2 hits.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene sequence and properties of CelI, a family E endoglucanase from Clostridium thermocellum."
      Hazlewood G.P., Davidson K., Laurie J.I., Huskisson N.S., Gilbert H.J.
      J. Gen. Microbiol. 139:307-316(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 56-69.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

    Entry informationi

    Entry nameiGUNI_CLOTH
    AccessioniPrimary (citable) accession number: Q02934
    Secondary accession number(s): A3DBF2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3