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Q02934 (GUNI_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 1

EC=3.2.1.4
Alternative name(s):
Cellulase I
Endo-1,4-beta-glucanase
Endoglucanase I
Short name=EGI
Gene names
Name:celI
Ordered Locus Names:Cthe_0040
OrganismClostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]
Taxonomic identifier203119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length887 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. Principally active against barley beta-glucan.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathway

Glycan metabolism; cellulose degradation.

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Contains 2 CBM3 (carbohydrate binding type-3) domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself4EBI-8601842,EBI-8601842

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5555 Ref.1
Chain56 – 887832Endoglucanase 1
PRO_0000007951

Regions

Domain529 – 684156CBM3 1
Domain736 – 887152CBM3 2
Region56 – 518463Catalytic

Sites

Active site4481 By similarity
Active site4861 By similarity
Active site4951 By similarity

Experimental info

Sequence conflict861 – 88727NPSAS…YGKEP → KQACLRQRTLIYLYATWLR in AAA20892. Ref.1

Secondary structure

........................................................................................... 887
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q02934 [UniParc].

Last modified April 17, 2007. Version 2.
Checksum: E587626445BA7A95

FASTA88798,531
        10         20         30         40         50         60 
MRLVNSLGRR KILLILAVIV AFSTVLLFAK LWGRKTSSTL DEVGSKTHGD LTAENKNGGY 

        70         80         90        100        110        120 
LPEEEIPDQP PATGAFNYGE ALQKAIFFYE CQRSGKLDPS TLRLNWRGDS GLDDGKDAGI 

       130        140        150        160        170        180 
DLTGGWYDAG DHVKFNLPMS YSAAMLGWAV YEYEDAFKQS GQYNHILNNI KWACDYFIKC 

       190        200        210        220        230        240 
HPEKDVYYYQ VGDGHADHAW WGPAEVMPME RPSYKVDRSS PGSTVVAETS AALAIASIIF 

       250        260        270        280        290        300 
KKVDGEYSKE CLKHAKELFE FADTTKSDDG YTAANGFYNS WSGFYDELSW AAVWLYLATN 

       310        320        330        340        350        360 
DSSYLDKAES YSDKWGYEPQ TNIPKYKWAQ CWDDVTYGTY LLLARIKNDN GKYKEAIERH 

       370        380        390        400        410        420 
LDWWTTGYNG ERITYTPKGL AWLDQWGSLR YATTTAFLAC VYSDWENGDK EKAKTYLEFA 

       430        440        450        460        470        480 
RSQADYALGS TGRSFVVGFG ENPPKRPHHR TAHGSWADSQ MEPPEHRHVL YGALVGGPDS 

       490        500        510        520        530        540 
TDNYTDDISN YTCNEVACDY NAGFVGLLAK MYKLYGGSPD PKFNGIEEVP EDEIFVEAGV 

       550        560        570        580        590        600 
NASGNNFIEI KAIVNNKSGW PARVCENLSF RYFINIEEIV NAGKSASDLQ VSSSYNQGAK 

       610        620        630        640        650        660 
LSDVKHYKDN IYYVEVDLSG TKIYPGGQSA YKKEVQFRIS APEGTVFNPE NDYSYQGLSA 

       670        680        690        700        710        720 
GTVVKSEYIP VYDAGVLVFG REPGSASKST SKDNGLSKAT PTVKTESQPT AKHTQNPASD 

       730        740        750        760        770        780 
FKTPANQNSV KKDQGIKGEV VLQYANGNAG ATSNSINPRF KIINNGTKAI NLSDVKIRYY 

       790        800        810        820        830        840 
YTKEGGASQN FWCDWSSAGN SNVTGNFFNL SSPKEGADTC LEVGFGSGAG TLDPGGSVEV 

       850        860        870        880 
QIRFSKEDWS NYNQSNDYSF NPSASDYTDW NRVTLYISNK LVYGKEP 

« Hide

References

« Hide 'large scale' references
[1]"Gene sequence and properties of CelI, a family E endoglucanase from Clostridium thermocellum."
Hazlewood G.P., Davidson K., Laurie J.I., Huskisson N.S., Gilbert H.J.
J. Gen. Microbiol. 139:307-316(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 56-69.
[2]"Complete sequence of Clostridium thermocellum ATCC 27405."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27405 / DSM 1237.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L04735 Genomic DNA. Translation: AAA20892.1.
CP000568 Genomic DNA. Translation: ABN51281.1.
PIRA47704.
RefSeqYP_001036474.1. NC_009012.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2XFGX-ray1.68A54-516[»]
B517-683[»]
ProteinModelPortalQ02934.
SMRQ02934. Positions 76-683.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-6946608.
STRING203119.Cthe_0040.

Protein family/group databases

CAZyCBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN51281; ABN51281; Cthe_0040.
GeneID4808805.
KEGGcth:Cthe_0040.
PATRIC19513685. VBICloThe47081_0045.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2730.
HOGENOMHOG000021032.
KOK01179.
K01225.
OMAFAVRQIN.
OrthoDBEOG6KQ6BP.

Enzyme and pathway databases

BioCycCTHE203119:GIW8-39-MONOMER.
UniPathwayUPA00696.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
2.60.40.710. 2 hits.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF00942. CBM_3. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTSM01067. CBM_3. 2 hits.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 2 hits.
PROSITEPS51172. CBM3. 2 hits.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ02934.

Entry information

Entry nameGUNI_CLOTH
AccessionPrimary (citable) accession number: Q02934
Secondary accession number(s): A3DBF2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: April 17, 2007
Last modified: April 16, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries