Reviewed, UniProtKB/Swiss-Prot Q02934 (GUNI_CLOTH)

Names and origin

Protein names

Recommended name:
    Endoglucanase 1
    EC=3.2.1.4
Alternative name(s):
    Endoglucanase I
      Short name=EGI
    Endo-1,4-beta-glucanase
    Cellulase I

Gene names

Name:	celI
Ordered Locus Names:	Cthe_0040

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. Principally active against barley beta-glucan.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Pathway	Glycan metabolism; cellulose degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family. Contains 2 CBM3 (carbohydrate binding type-3) domains.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 55	55	Ref.1
	Chain	56 – 887	832	Endoglucanase 1		PRO_0000007951
Regions
	Domain	529 – 684	156	CBM3 1
	Domain	736 – 887	152	CBM3 2
	Region	56 – 518	463	Catalytic
Sites
	Active site	448	1	By similarity
	Active site	486	1	By similarity
	Active site	495	1	By similarity
Experimental info
	Sequence conflict	861 – 887	27	NPSAS…YGKEP → KQACLRQRTLIYLYATWLR in AAA20892. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q02934-1 [UniParc]. Last modified April 17, 2007. Version 2. Checksum: E587626445BA7A95	FASTA	887	98,531
	10 20 30 40 50 60 MRLVNSLGRR KILLILAVIV AFSTVLLFAK LWGRKTSSTL DEVGSKTHGD LTAENKNGGY 70 80 90 100 110 120 LPEEEIPDQP PATGAFNYGE ALQKAIFFYE CQRSGKLDPS TLRLNWRGDS GLDDGKDAGI 130 140 150 160 170 180 DLTGGWYDAG DHVKFNLPMS YSAAMLGWAV YEYEDAFKQS GQYNHILNNI KWACDYFIKC 190 200 210 220 230 240 HPEKDVYYYQ VGDGHADHAW WGPAEVMPME RPSYKVDRSS PGSTVVAETS AALAIASIIF 250 260 270 280 290 300 KKVDGEYSKE CLKHAKELFE FADTTKSDDG YTAANGFYNS WSGFYDELSW AAVWLYLATN 310 320 330 340 350 360 DSSYLDKAES YSDKWGYEPQ TNIPKYKWAQ CWDDVTYGTY LLLARIKNDN GKYKEAIERH 370 380 390 400 410 420 LDWWTTGYNG ERITYTPKGL AWLDQWGSLR YATTTAFLAC VYSDWENGDK EKAKTYLEFA 430 440 450 460 470 480 RSQADYALGS TGRSFVVGFG ENPPKRPHHR TAHGSWADSQ MEPPEHRHVL YGALVGGPDS 490 500 510 520 530 540 TDNYTDDISN YTCNEVACDY NAGFVGLLAK MYKLYGGSPD PKFNGIEEVP EDEIFVEAGV 550 560 570 580 590 600 NASGNNFIEI KAIVNNKSGW PARVCENLSF RYFINIEEIV NAGKSASDLQ VSSSYNQGAK 610 620 630 640 650 660 LSDVKHYKDN IYYVEVDLSG TKIYPGGQSA YKKEVQFRIS APEGTVFNPE NDYSYQGLSA 670 680 690 700 710 720 GTVVKSEYIP VYDAGVLVFG REPGSASKST SKDNGLSKAT PTVKTESQPT AKHTQNPASD 730 740 750 760 770 780 FKTPANQNSV KKDQGIKGEV VLQYANGNAG ATSNSINPRF KIINNGTKAI NLSDVKIRYY 790 800 810 820 830 840 YTKEGGASQN FWCDWSSAGN SNVTGNFFNL SSPKEGADTC LEVGFGSGAG TLDPGGSVEV 850 860 870 880 QIRFSKEDWS NYNQSNDYSF NPSASDYTDW NRVTLYISNK LVYGKEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene sequence and properties of CelI, a family E endoglucanase from Clostridium thermocellum." Hazlewood G.P., Davidson K., Laurie J.I., Huskisson N.S., Gilbert H.J. J. Gen. Microbiol. 139:307-316(1993) [PubMed: 8436949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 56-69.
[2]	"Complete sequence of Clostridium thermocellum ATCC 27405." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L04735 Genomic DNA. Translation: AAA20892.1. CP000568 Genomic DNA. Translation: ABN51281.1.
PIR	A47704.
RefSeq	YP_001036474.1.
3D structure databases
HSSP	HSSP built from PDB template 1G87 based on UniProtKB P37700.
ModBase	Search...
Protein family/group databases
CAZy	CBM3. Carbohydrate-Binding Module Family 3. GH9. Glycoside Hydrolase Family 9.
Genome annotation databases
GeneID	4808805.
GenomeReviews	Gene locus Cthe_0040 in contig CP000568_GR.
KEGG	cth:Cthe_0040.
NMPDR	fig|203119.1.peg.878.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q02934. GGQSAYK.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001956. CBD_3. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:2.60.40.710. CBD_3. 2 hits. G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00942. CBM_3. 2 hits. PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
ProDom	PD001947. CBD_3. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51172. CBM3. 2 hits. PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNI_CLOTH

Accession

Primary (citable) accession number: Q02934
Secondary accession number(s): A3DBF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	April 17, 2007
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)