Reviewed, UniProtKB/Swiss-Prot Q02932 (KA120_YEAST)

Names and origin

Protein names

Recommended name:
    Importin beta-like protein KAP120
Alternative name(s):
    Karyopherin-120

Gene names

Name:	KAP120
Ordered Locus Names:	YPL125W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1032 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, RAN-dependent mechanism. Required for nuclear import of Ho endonuclease and RFP1, and involved in rRNA-processing and assembly or export of 60S ribosomal subunits. Ref.3 Ref.4 Ref.7 Ref.8
Subunit structure	Interacts with GTP-bound GSP1 and RFP1. Associates with the nuclear pore complex. Ref.8
Subcellular location	Cytoplasm. Nucleus.
Miscellaneous	Present with 6300 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the importin beta family. Contains 1 importin N-terminal domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein import into nucleus Ref.2 Inferred from physical interaction. Source: SGD
Cellular component	cytoplasm Ref.2 Inferred from direct assay. Source: SGD nuclear pore Ref.2 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein transporter activity Inferred from electronic annotation. Source: InterPro structural constituent of nuclear pore Ref.2 Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-33816,EBI-24597
SAM2	P19358	1	EBI-33816,EBI-10795

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1032	1032	Importin beta-like protein KAP120		PRO_0000255954
Regions
	Domain	31 – 103	73	Importin N-terminal

Sequences

	Sequence		Length	Mass (Da)
	Q02932-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 493EDA2B43298F10	FASTA	1,032	119,623
	10 20 30 40 50 60 MASSLNELNL VQVLEQASNP QHIRSDVQKL AEQQLRQWET QAGFHYLLQS IYLNLSNSLQ 70 80 90 100 110 120 IRWLAVIQFK NGVDKYWRST RINAIPKDEK ASIRGRLFEM IDEQNNQLCI QNAQASARIA 130 140 150 160 170 180 RLDFPVEWPT LFEDLENLLN DEIIRKDSVK IYNILMHINQ IVKVLGTARI GRCRPAMQSK 190 200 210 220 230 240 VPLILPLIVR IYLQSFEEWT TSSNLNYEDL SSLQVSYLAL KVLRRIICEG YDRPQTDQSV 250 260 270 280 290 300 CDFIKLSVSH FEMLISNHEN FKKFDIYEKF IKCLGKLYFN LVTGSPANFI LLPCSTQILI 310 320 330 340 350 360 TYTRLIFDKA PKVYRENSDV TGDFWEQTAI RGLLILKRVI NFIHKKGAIT LKARSDKLTI 370 380 390 400 410 420 DASINKINTE FLNENLITRL VDTLMEWYLR LRPTELENWF MDPEEWINEQ MATSYEYQIR 430 440 450 460 470 480 PCAENVFQDL MNTFSELLVP YLLKKIENDA SKLSNSLDDF LRKDAIYASF QLSASAVSEM 490 500 510 520 530 540 VDFDRLLIQV FLPEATNTNI SGDELRIIRR RVALIINEWS TVKCSEESKS LCYKLFTNFL 550 560 570 580 590 600 TDEDDKVVLL TTVQTVRTMV DDWNFNKDTF QPFLTENVHL LLRKILPSVS LTETRLYVLN 610 620 630 640 650 660 TLSDIIIQTK PLISRDLLVE ILQIIPNLWE IATNNASEAI LANALLRLLR NLVSSLGSQS 670 680 690 700 710 720 HLTWDIAIPV VALACDPSSM QYQLLSEDGY ELWGMLLQNF SSHDQEFDDK FVELVPFLKY 730 740 750 760 770 780 GIETHTEILP TLLEIIKSYA LILNPVDFFS NNTFQDIFKQ MSKYLLKLRE DSFQLVLEIW 790 800 810 820 830 840 EILILSNESD YENLLLQKFY ETGVLSALFD AIFLEEAPSS YLCSQIIQII ARISYVNPDA 850 860 870 880 890 900 LMTFLATYHD NLPTSNENAR MPESIRKIVS KDQTYDSVVN KLLTGWIVCF RDIFDPKFKK 910 920 930 940 950 960 VHILGISSLL RTGLVPILTE FSSIASLWIE MLEEINETNR GDCEKYHLND IVTEQSIAFH 970 980 990 1000 1010 1020 PLTAEQLRYH QLCKNNDPVH NISLKDFISQ SMEYLESHLG VERYQEFLKT INPSLLENLQ 1030 MFLSIQPQEA RP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The yeast nuclear pore complex: composition, architecture, and transport mechanism." Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T. J. Cell Biol. 148:635-651(2000) [PubMed: 10684247] [Abstract] Cited for: ASSOCIATION WITH THE NUCLEAR PORE COMPLEX, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[3]	"Factors affecting nuclear export of the 60S ribosomal subunit in vivo." Stage-Zimmermann T., Schmidt U., Silver P.A. Mol. Biol. Cell 11:3777-3789(2000) [PubMed: 11071906] [Abstract] Cited for: FUNCTION.
[4]	"The budding index of Saccharomyces cerevisiae deletion strains identifies genes important for cell cycle progression." Zettel M.F., Garza L.R., Cass A.M., Myhre R.A., Haizlip L.A., Osadebe S.N., Sudimack D.W., Pathak R., Stone T.L., Polymenis M. FEMS Microbiol. Lett. 223:253-258(2003) [PubMed: 12829295] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Nuclear import of Ho endonuclease utilizes two nuclear localization signals and four importins of the ribosomal import system." Bakhrat A., Baranes K., Krichevsky O., Rom I., Schlenstedt G., Pietrokovski S., Raveh D. J. Biol. Chem. 281:12218-12226(2006) [PubMed: 16507575] [Abstract] Cited for: FUNCTION.
[8]	"Kap120 functions as a nuclear import receptor for ribosome assembly factor Rpf1 in yeast." Caesar S., Greiner M., Schlenstedt G. Mol. Cell. Biol. 26:3170-3180(2006) [PubMed: 16581791] [Abstract] Cited for: FUNCTION, INTERACTION WITH GSP1 AND RFP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U43503 Genomic DNA. Translation: AAB68237.1.
PIR	S61997.
RefSeq	NP_015200.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4000N.
IntAct	Q02932. 15 interactions.
STRING	Q02932.
Proteomic databases
PeptideAtlas	Q02932.
Genome annotation databases
Ensembl	YPL125W; YPL125W; YPL125W; Saccharomyces cerevisiae. [Genome view]
GeneID	855978.
KEGG	sce:YPL125W.
NMPDR	fig|4932.3.peg.6331.
Organism-specific databases
CYGD	YPL125w.
SGD	S000006046. KAP120.
Phylogenomic databases
HOGENOM	Q02932.
OMA	TESKLYI
OrthoDB	EOG9X6DRW
Gene expression databases
ArrayExpress	Q02932.
Genevestigator	Q02932.
GermOnline	YPL125W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR001494. Importin-b_N. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF03810. IBN_N. 1 hit. [Graphical view]
PROSITE	PS50166. IMPORTIN_B_NT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	980807.

Entry information

Entry name

KA120_YEAST

Accession

Primary (citable) accession number: Q02932

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)