ID   UTP17_YEAST             Reviewed;         896 AA.
AC   Q02931; D6W3P1;
DT   01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 182.
DE   RecName: Full=NET1-associated nuclear protein 1;
DE   AltName: Full=U three protein 17;
DE            Short=t-17;
DE   AltName: Full=U3 protein 17 required for transcription;
DE   AltName: Full=U3 small nucleolar RNA-associated protein 17;
DE            Short=U3 snoRNA-associated protein 17;
GN   Name=NAN1; Synonyms=UTP17; OrderedLocusNames=YPL126W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169875;
RA   Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA   Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA   Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA   Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA   DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA   Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA   Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA   Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA   Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA   Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA   Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA   Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA   Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA   Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA   Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA   Vo D.H., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL   Nature 387:103-105(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU
RP   PROCESSOME BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX   PubMed=12068309; DOI=10.1038/nature00769;
RA   Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M.,
RA   Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J.,
RA   Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J.;
RT   "A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA
RT   biogenesis.";
RL   Nature 417:967-970(2002).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=15489292; DOI=10.1101/gad.1226604;
RA   Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y.,
RA   Beyer A.L., Baserga S.J.;
RT   "RNA polymerase I transcription and pre-rRNA processing are linked by
RT   specific SSU processome components.";
RL   Genes Dev. 18:2506-2517(2004).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA.
CC       Required for optimal pre-ribosomal RNA transcription by RNA polymerase
CC       I together with a subset of U3 proteins required for transcription (t-
CC       UTPs). {ECO:0000269|PubMed:12068309, ECO:0000269|PubMed:15489292}.
CC   -!- SUBUNIT: Interacts with snoRNA U3. Interacts with MPP10. Component of
CC       the ribosomal small subunit (SSU) processome composed of at least 40
CC       protein subunits and snoRNA U3. In the absence of snoRNA3, forms a
CC       complex with other t-UTPs. This complex can associate with pre-18S
CC       ribosomal RNAs. {ECO:0000269|PubMed:12068309,
CC       ECO:0000269|PubMed:15489292}.
CC   -!- INTERACTION:
CC       Q02931; P42945: UTP10; NbExp=7; IntAct=EBI-37773, EBI-1884;
CC       Q02931; P53276: UTP8; NbExp=5; IntAct=EBI-37773, EBI-23301;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12068309,
CC       ECO:0000269|PubMed:15489292}. Note=Associated with ribosomal chromatin,
CC       even in the absence of transcription.
CC   -!- MISCELLANEOUS: Present with 6560 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; U43503; AAB68236.1; -; Genomic_DNA.
DR   EMBL; BK006949; DAA11307.1; -; Genomic_DNA.
DR   PIR; S61996; S61996.
DR   RefSeq; NP_015199.1; NM_001183940.1.
DR   PDB; 5WLC; EM; 3.80 A; LH=1-896.
DR   PDB; 6KE6; EM; 3.40 A; AG=1-896.
DR   PDB; 6LQP; EM; 3.20 A; AG=1-896.
DR   PDB; 6LQQ; EM; 4.10 A; AG=1-896.
DR   PDB; 6LQR; EM; 8.60 A; AG=1-896.
DR   PDB; 6LQS; EM; 3.80 A; AG=1-896.
DR   PDB; 6LQT; EM; 4.90 A; AG=1-896.
DR   PDB; 6LQU; EM; 3.70 A; AG=1-896.
DR   PDB; 6LQV; EM; 4.80 A; AG=1-896.
DR   PDB; 6ND4; EM; 4.30 A; H=1-896.
DR   PDB; 6ZQA; EM; 4.40 A; UQ=1-896.
DR   PDB; 6ZQB; EM; 3.90 A; UQ=1-896.
DR   PDB; 6ZQC; EM; 3.80 A; UQ=1-896.
DR   PDB; 6ZQD; EM; 3.80 A; UQ=1-896.
DR   PDB; 6ZQE; EM; 7.10 A; UQ=1-896.
DR   PDB; 7AJT; EM; 4.60 A; UQ=1-896.
DR   PDB; 7AJU; EM; 3.80 A; UQ=1-896.
DR   PDB; 7D4I; EM; 4.00 A; AG=1-896.
DR   PDB; 7D5S; EM; 4.60 A; AG=1-896.
DR   PDB; 7D5T; EM; 6.00 A; AG=1-896.
DR   PDB; 7D63; EM; 12.30 A; AG=1-896.
DR   PDB; 7SUK; EM; 3.99 A; LH=1-896.
DR   PDBsum; 5WLC; -.
DR   PDBsum; 6KE6; -.
DR   PDBsum; 6LQP; -.
DR   PDBsum; 6LQQ; -.
DR   PDBsum; 6LQR; -.
DR   PDBsum; 6LQS; -.
DR   PDBsum; 6LQT; -.
DR   PDBsum; 6LQU; -.
DR   PDBsum; 6LQV; -.
DR   PDBsum; 6ND4; -.
DR   PDBsum; 6ZQA; -.
DR   PDBsum; 6ZQB; -.
DR   PDBsum; 6ZQC; -.
DR   PDBsum; 6ZQD; -.
DR   PDBsum; 6ZQE; -.
DR   PDBsum; 7AJT; -.
DR   PDBsum; 7AJU; -.
DR   PDBsum; 7D4I; -.
DR   PDBsum; 7D5S; -.
DR   PDBsum; 7D5T; -.
DR   PDBsum; 7D63; -.
DR   PDBsum; 7SUK; -.
DR   AlphaFoldDB; Q02931; -.
DR   EMDB; EMD-0949; -.
DR   EMDB; EMD-0950; -.
DR   EMDB; EMD-0951; -.
DR   EMDB; EMD-0952; -.
DR   EMDB; EMD-0953; -.
DR   EMDB; EMD-0954; -.
DR   EMDB; EMD-0955; -.
DR   EMDB; EMD-11357; -.
DR   EMDB; EMD-11358; -.
DR   EMDB; EMD-11359; -.
DR   EMDB; EMD-11360; -.
DR   EMDB; EMD-11361; -.
DR   EMDB; EMD-11807; -.
DR   EMDB; EMD-11808; -.
DR   EMDB; EMD-30574; -.
DR   EMDB; EMD-30584; -.
DR   EMDB; EMD-30585; -.
DR   EMDB; EMD-30588; -.
DR   EMDB; EMD-9964; -.
DR   SMR; Q02931; -.
DR   BioGRID; 36055; 139.
DR   ComplexPortal; CPX-1409; UTP-A complex.
DR   DIP; DIP-3999N; -.
DR   IntAct; Q02931; 47.
DR   MINT; Q02931; -.
DR   STRING; 4932.YPL126W; -.
DR   iPTMnet; Q02931; -.
DR   MaxQB; Q02931; -.
DR   PaxDb; 4932-YPL126W; -.
DR   PeptideAtlas; Q02931; -.
DR   EnsemblFungi; YPL126W_mRNA; YPL126W; YPL126W.
DR   GeneID; 855977; -.
DR   KEGG; sce:YPL126W; -.
DR   AGR; SGD:S000006047; -.
DR   SGD; S000006047; NAN1.
DR   VEuPathDB; FungiDB:YPL126W; -.
DR   eggNOG; KOG1963; Eukaryota.
DR   HOGENOM; CLU_348179_0_0_1; -.
DR   InParanoid; Q02931; -.
DR   OMA; KWHIDSV; -.
DR   OrthoDB; 927410at2759; -.
DR   BioCyc; YEAST:G3O-34025-MONOMER; -.
DR   Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR   BioGRID-ORCS; 855977; 1 hit in 10 CRISPR screens.
DR   PRO; PR:Q02931; -.
DR   Proteomes; UP000002311; Chromosome XVI.
DR   RNAct; Q02931; Protein.
DR   GO; GO:0030686; C:90S preribosome; HDA:SGD.
DR   GO; GO:0005730; C:nucleolus; IDA:SGD.
DR   GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR   GO; GO:0033553; C:rDNA heterochromatin; IDA:SGD.
DR   GO; GO:0032040; C:small-subunit processome; IDA:SGD.
DR   GO; GO:0034455; C:t-UTP complex; IDA:SGD.
DR   GO; GO:0003729; F:mRNA binding; HDA:SGD.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0034511; F:U3 snoRNA binding; IDA:SGD.
DR   GO; GO:0030490; P:maturation of SSU-rRNA; NAS:ComplexPortal.
DR   GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR   GO; GO:2000234; P:positive regulation of rRNA processing; IBA:GO_Central.
DR   GO; GO:0045943; P:positive regulation of transcription by RNA polymerase I; IMP:SGD.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 2.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR001680; WD40_rpt.
DR   PANTHER; PTHR44215; WD REPEAT-CONTAINING PROTEIN 75; 1.
DR   PANTHER; PTHR44215:SF1; WD REPEAT-CONTAINING PROTEIN 75; 1.
DR   Pfam; PF00400; WD40; 1.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50993; Peptidase/esterase 'gauge' domain; 1.
DR   SUPFAM; SSF50978; WD40 repeat-like; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Nucleus; Reference proteome; Repeat; Ribonucleoprotein;
KW   Ribosome biogenesis; rRNA processing; Transcription; WD repeat.
FT   CHAIN           1..896
FT                   /note="NET1-associated nuclear protein 1"
FT                   /id="PRO_0000051088"
FT   REPEAT          295..334
FT                   /note="WD 1"
FT   REPEAT          490..542
FT                   /note="WD 2"
FT   REPEAT          552..595
FT                   /note="WD 3"
FT   REPEAT          605..645
FT                   /note="WD 4"
SQ   SEQUENCE   896 AA;  101237 MW;  F4E3687BD328230E CRC64;
     MTQSLGIEQY KLSVVSGGKP ALNNLSSVTG NKNIARLSQD QRNYIIPFNN QIKVYSVETR
     QCVKTLKFAN NSLLSGIFLQ EEENNESIVK ILLGDITVPQ QEDAHLITVF TNNGHVIVLN
     YKGKLVESPK HFKISLADEK LANVFHSEGN YRILTTFKDP SQKAHNSLQS YRLYALTFDD
     AKKQFEVAHQ AEWHNVILSN ISSNGKLLAH MCKDVSTKDH EHKSISVVSL FDDSVNLSFP
     LGSILSSQTQ SLSYNTRYVS SMAIDNMGQQ LAVGFASGVI SIVSLADLQI RLLKWHIDSV
     LSLSFSHDGS YLLSGGWEKV MSLWQLETNS QQFLPRLNGI IIDCQVLGPQ GNYYSLILQM
     TENNSNSDYQ FLLLNASDLT SKLSINGPLP VFNSTIKHIQ QPISAMNTKN SNSITSLNHS
     KKKQSRKLIK SRRQDFTTNV EINPINKNLY FPHISAVQIF DFYKNEQVNY QYLTSGVNNS
     MGKVRFELNL QDPIITDLKF TKDGQWMITY EIEYPPNDLL SSKDLTHILK FWTKNDNETN
     WNLKTKVINP HGISVPITKI LPSPRSVNNS QGCLTADNNG GLKFWSFDSH ESNWCLKKIS
     LPNFNHFSNS VSLAWSQDGS LIFHGFDDKL QILDFDTFKK FESLENTKTV SEFTLDSEIQ
     TVKLINDTNL IVATRTTLNA INLLRGQVIN SFDLYPFVNG VYKNGHMDRL ITCDERTGNI
     ALVINQQLTD LDGVPTINYK SRIIIFDSDL STKLGNFTHH EYISWIGWNY DTDFIFLDIE
     STLGVVGTTV NTQLSDEVNN EGILDGLVSN TITTSASNSD IFAEQLHKLS SRGKKSDTRD
     KNTNDNDEDE EDIALEFING EKKDKLVNMN SFTSMFDNIQ NVQMDTFFDR VMKVLT
//