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Protein

Elongator complex protein 3

Gene

ELP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as catalytic subunit of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. ELP3 is required for the complex integrity and for the association of the complex with nascent RNA transcript. Independently, ELP3 may be involved in polarized exocytosis. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.9 Publications

Miscellaneous

Present with 4760 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Metal bindingi118Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Metal bindingi121Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1

GO - Molecular functioni

  • histone acetyltransferase activity Source: SGD
  • iron-sulfur cluster binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • DNA mediated transformation Source: EnsemblPlants
  • protein transport Source: UniProtKB-KW
  • regulation of auxin mediated signaling pathway Source: EnsemblPlants
  • regulation of leaf formation Source: EnsemblPlants
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • tRNA wobble uridine modification Source: SGD

Keywordsi

Molecular functionAcyltransferase, Chromatin regulator, Transferase
Biological processProtein transport, Transcription, Transcription regulation, Transport
LigandIron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-33992-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongator complex protein 3 (EC:2.3.1.48)
Alternative name(s):
Gamma-toxin target 3
Gene namesi
Name:ELP3
Synonyms:HPA1, TOT3
Ordered Locus Names:YPL086C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL086C.
SGDiS000006007. ELP3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi108C → S: Eliminates iron contents; when associated with S-118 and S-121. 1 Publication1
Mutagenesisi118C → S: Eliminates iron contents; when associated with S-108 and S-121. 1 Publication1
Mutagenesisi121C → S: Eliminates iron contents; when associated with S-108 and S-118. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002358111 – 557Elongator complex protein 3Add BLAST557

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki453Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ02908.
PRIDEiQ02908.

PTM databases

iPTMnetiQ02908.

Interactioni

Subunit structurei

Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. IKI3, ELP2, and ELP3 form the Elongator core complex. ELP3 interacts with KTI12.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36095. 501 interactors.
DIPiDIP-2385N.
IntActiQ02908. 21 interactors.
MINTiMINT-645857.
STRINGi4932.YPL086C.

Structurei

3D structure databases

ProteinModelPortaliQ02908.
SMRiQ02908.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini405 – 557N-acetyltransferasePROSITE-ProRule annotationAdd BLAST153

Sequence similaritiesi

Belongs to the ELP3 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000013141.
HOGENOMiHOG000227514.
InParanoidiQ02908.
KOiK07739.
OMAiKIVAHMM.
OrthoDBiEOG092C1F4W.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR000182. GNAT_dom.
IPR005910. Hist_AcTrfase_ELP3.
IPR032432. Radical_SAM_C.
IPR007197. rSAM.
PfamiView protein in Pfam
PF00583. Acetyltransf_1. 1 hit.
PF04055. Radical_SAM. 1 hit.
PF16199. Radical_SAM_C. 1 hit.
PIRSFiPIRSF005669. Hist_AcTrfase_ELP3. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR01211. ELP3. 1 hit.
PROSITEiView protein in PROSITE
PS51186. GNAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02908-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARHGKGPKT NKKKLAPEKE RFIQCCADIT LELTDSLTSG TTREINLNGL
60 70 80 90 100
ITKYSKKYKL KQQPRLTDII NSIPDQYKKY LLPKLKAKPV RTASGIAVVA
110 120 130 140 150
VMCKPHRCPH IAYTGNICVY CPGGPDSDFE YSTQSYTGYE PTSMRAIRAR
160 170 180 190 200
YDPYEQARGR VEQLKQLGHS IDKVEYVLMG GTFMSLPKEY REDFIVKLHN
210 220 230 240 250
ALSGFNGNDI DEAILYSQQS LTKCVGITIE TRPDYCTQTH LDDMLKYGCT
260 270 280 290 300
RLEIGVQSLY EDVARDTNRG HTVRSVCETF AVSKDAGYKV VSHMMPDLPN
310 320 330 340 350
VGMERDIEQF KEYFENPDFR TDGLKIYPTL VIRGTGLYEL WKTGRYKSYS
360 370 380 390 400
ANALVDLVAR ILALVPPWTR IYRVQRDIPM PLVTSGVDNG NLRELALARM
410 420 430 440 450
KDLGTTCRDV RTREVGIQEV HHKVQPDQVE LIRRDYYANG GWETFLSYED
460 470 480 490 500
PKKDILIGLL RLRKASKKYT YRKEFTSQRT SIVRELHVYG SVVPLHSRDP
510 520 530 540 550
RKFQHQGFGT LLMEEAERIA KEEHGSEKIS VISGVGVRNY YGKLGYELDG

PYMSKRI
Length:557
Mass (Da):63,657
Last modified:November 1, 1996 - v1
Checksum:i64EF8B42D3C3102E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43281 Genomic DNA. Translation: AAB68213.1.
BK006949 Genomic DNA. Translation: DAA11347.1.
PIRiS61980.
RefSeqiNP_015239.1. NM_001183900.1.

Genome annotation databases

EnsemblFungiiYPL086C; YPL086C; YPL086C.
GeneIDi856019.
KEGGisce:YPL086C.

Similar proteinsi

Entry informationi

Entry nameiELP3_YEAST
AccessioniPrimary (citable) accession number: Q02908
Secondary accession number(s): D6W3T1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names